• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 1996년도 추계학술대회논문집; 한국과학기술회관, 8 Nov. 1996
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• pp.431-437
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• 1996

The natural frequencies of annular plates in contact with water were theoretically derived and its validity was checked by experiments. The experimental frequency response functions of the annular plates were obtained using the impact hammer method. Comparison of the FRF obtained using the impact hammer and the fRF using shaker attached showed that the former was better than the latter due to the mass effect and additional constraint from the instrumentation. It was found that the experimentally extracted natural frequencies of annular plates in contact with water were in good agreement with those theoretical values.

• Advances in nano research
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• 제10권3호
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• pp.295-312
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• 2021

A new ionic liquid functionalized magnetic silica nanoparticle was synthesized and characterized and tested as an adsorbent. The adsorbent was used for magnetic solid phase extraction on ICP-MS method. Simultaneous determination of precious metal Au has been addressed. The method is simple and fast and has been applied to standard water and surface water analysis. A new method for separation/analysis of trace precious metal Au by Magnetron Solid Phase Extraction (MSPE) combined with ICP-MS. The element to be tested is rapidly adsorbed on CoFe2O4@SiO2@[BMIM]PF6 composite nano-adsorbent and eluted with thiourea. The method has a preconcentration factor of 9.5-fold. This method has been successfully applied to the determination of gold in actual water samples. Hydrophobic Ionic Liquids (ILs) 1-butyl-3-methylimidazole hexafluorophosphate ([BMIM]PF6) coated CoFe2O4@SiO2 nanoparticles with core-shell structure to prepare magnetic solid phase extraction agent (CoFe2O4@SiO2@ILs) and establish a new method of MSPE coupled with inductively coupled plasma mass spectrometry for separation/analysis of trace gold. The results showed that trace gold was adsorbed rapidly by CoFe2O4@SiO2@[BMIM]PF6 and eluanted by thiourea. Under the optimal conditions, preconcentration factor of the proposed method was 9.5-fold. The linear range, detection limit, correlation coefficient (R) and relative standard deviation (RSD) were found to be 0.01~1000.00 ng·mL-1, 0.001 ng·mL-1, 0.9990 and 3.4% (n = 11, c = 4.5 ng·mL-1). The CoFe2O4@SiO2 nanoparticles could be used repeatedly for 8 times. This proposed method has been successfully applied to the determination of trace gold in water samples.

• 터널과지하공간
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• 제18권1호
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• pp.44-57
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• 2008

본 연구에서는 암반의 수리적 거동을 다공성매체로 간주하고 개별균열 연결망을 통해 구해진 암반의 등가수리전도도와 수리이방성을 입력 자료로 하여 유한요소법 프로그램을 사용한 2차원 수리해석을 수행하였다. 입력변수에 의한 결정론적인 해를 얻게 되는 연속체 해석의 단점과 입력자료의 불확실성을 보완하기 위하여 입력변수에 대한 민감도 분석을 실시하고, 터널과 단층파쇄대의 다양한 위치관계를 고려한 가상의 시나리오 단면에 대하여 해석을 수행하였다. 이때 암반의 포화 불포화 특성을 반영하기 위한 수리전도도함수와 체적함수율 함수를 구하여 해석에 반영하였다. 해석대상지역은 경부고속철도 13-3공구, 원효터널의 고산 습지(무제치 3늪, 대성뒷늪, 대성큰늪) 하부통과구간과 간천계곡하부 통과구간으로서 부정류 해석을 통하여 터널 굴착 후 10년 경과시까지의 터널 내 유입수량과 인접지역의 지하수위를 살펴봄으로써 수리학적 안정성과 습지 및 계곡에 미치는 영향을 검토하였다. 그라우팅을 시행하지 않은 경우에는 무제치늪과 대성뒷늪 주변의 지하수위가 강하하지만, 그라우팅을 통해 수리전도도를 1/50 이하로 감소시킬 경우 지하수위는 강하하지 않는 것으로 나타났다. 그리고 단층파쇄대가 터널에 인접하여 위치하더라도 터널을 관통하지 않는 경우에는 단층파쇄대가 터널내 유입수량에 미치는 영향은 크지 않은 것으로 나타났다.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2003년도 추계학술대회 논문집
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• pp.340-345
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• 2003

This study, as a basic study for establishing a influence forecasting/estimating model when drain the deep sea water to the ocean after using it, carried out studies as follows; 1) estimating the amount of river discharge and pollutant loads inflowing into the developing region of deep sea water in East Sea, Korea 2) a field observation of tidal current, vertical distribution of water temperature and salinity, and 3-D numerical experiment of tidal current to analysis physical oceanographic status. The amount of river discharge flowing into the study area was estimated about $462.6{times}10^{3}m^{3}/day$ of daily mean in 2002 year. annual mean pollutant load of COD, TN and TP were estimated 7.02 ton-COD/day, 4.06 ton-TN/day and 0.39 ton/day, respectively. Field observation of tidal current results usually show about $20{\sim}40cm/sec$ of current velocity at the surface layer, it indicated a tendency that the current velocity decreases under 20cm/sec as the water depth increases. We could find a stratification within approximately the depth of 30m in field observation area, and the depth increases. We could find a stratification within approximately the depth of 30m in field observation area, and the differences of water temperature and salinity between the surface layer and bottom layer were about $18^{\circ}C$ and 0.8 psu, respectively. On the other hand, we found that there was a definite as the water mass of deep sea water about 34 psu of salinity.

• 대한지하수환경학회지
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• 제3권2호
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• pp.51-59
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• 1996

지하 LPG 저장시설과 같은 지하수에 의존하는 시설물 인접지역에 폐기물매립장이 설치될 경우, 침출수 누출로 인한 지하수오염 예측 및 오염방지 대책수립이 요구되고, 이를 위한 지하수유동 및 용질 이동(Mass Transport) 해석을 실시하게 된다. 본 연구에서는 현재 U 지역 석유화학공단내에서 발생된 상기 와 같은 내용의 문제점을 해결하기 위하여 해석학적 해석 및 수치해석에 의한 침출수이동 예측을 실시하였으며 침출수제어 공법을 검토하였다. 해석학적 해석은 입력요소를 이송(Advenction)요인과 분산 (Dispersion)요인으로 구분하여, Peclet 수로 부터 각 요인의 침출수이동에 미치는 영향을 분석 검토하였으며, 보존성용질 이송확산 방정식으로부터 입력요소 변화율과 침출수이동속도 변화율의 관계를 함수식으로 도출하여, 입력요소 변화에 따른 침출수 이동속도 변화를 간단히 예측할 수 있었다. 수치해석은 지하수유동 및 용질이동 해석용 FEM 프로그램인 AQUA2D를 이용하여 침출수이동 예측을 위한 수치모의를 실시하였다. 침출수제어공법 검토를 위해서 3D 불연속체모형을 모의하고 모의된 모형을 대상으로 지하수유동해석을 실시하여 암반내 열극(Fracture)의 상호 연결성 분석을 통한 수벽(Water Curtain) 시스템의 타당성을 분석하였다. 해석학적 해석 및 수치모의 결과, 폐기물매립장으로 부터 지하 LPG 저장기지로 침출수가 30년 이내로 유입되는 것으로 예측되었으며, 이를 방지하기 위한 침출수 제어공법으로는 수직수벽 시스템이 효율적인 것으로 예측되었다.

• 대한기계학회논문집A
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• 제37권6호
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• pp.739-745
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• 2013

본 연구에서는 급수 순환 시스템 중 동작 유체의 이송을 위해 사용하는 배관을 대상으로 관로 크기 및 고온 급수 환경에 따른 침식-부식 거동과 그 영향을 규명하고자 하였다. 이를 위해 일반적인 관로 소재인 철과 핵심 부식 인자인 산소의 화학반응식을 기반으로 Hayduk 과 Minhas 가 제안한 모델을 이용하여 침식-부식 해석을 실시하였다. 상용 유한요소해석 프로그램인 ABAQUS 를 사용하여 해석을 수행하였으며 배관의 직경 및 급수 온도를 변화시킴에 따른 침식-부식률을 평가할 수 있었다. 결과를 통해 급수 온도가 침식-부식률에 가장 큰 영향을 미치는 요인이 됨을 알 수 있었으며, 특히 $290^{\circ}C$ 급수에 노출된 스테인리스 316 강은 연간 $2.59{\mu}m$의 두께 손실이 발생할 것으로 예상되었다.

• 생약학회지
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• 제44권4호
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• pp.350-361
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• 2013

Asarum sieboldii has been used for treatment of fever, pain, common cold, and chronic sinusitis in Korea. In this study, we performed quantification analysis of seven major constituents including aristolochic acid I, aristolochic acid II, ${\alpha}$-asarone, ${\beta}$-asarone, elemicin, methyl eugenol, and safrole in the 70% ethanol extract of Asarum sieboldii and its solvent fractions, n-hexane, ethylacetate, n-butanol, and water ones using a ultra-performance liquid chromatography-electrospray ionization-mass spectrometer(UPLC-ESI-MS) and gas chromatography-mass spectrometer(GC-MS). Regression equations of seven components were acquired with $r^2$ values >0.99. The values of limit of detection(LOD) and quantification(LOQ) were 0.1-3.9 ng/mL and 0.3-11.7 mg/mL, respectively. The amount of the seven compounds in Asarum sieboldii were not detected -143.66 mg/g. The established LC-MS/MS and GC-MS methods will be helpful to improve quality control of Asarum sieboldii.

• Natural Product Sciences
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• 제20권3호
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• pp.182-190
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• 2014

An ultra-performance liquid chromatography (UPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS) method was established for quantitative analysis of twelve components, allantoin (1), morroniside (2), 5-hydroxymethyl-2-furfural (5-HMF) (3), loganin (4), coumarin (5), cinnamic acid (6), mesaconitine (7), cinnamaldehyde (8), hypaconitine (9), aconitine (10), alisol B (11), and alisol B acetate (12) in a Palmijihwang-hwan decoction. The twelve constituents were separated on a UPLC BEH C18 column ($2.1{\times}100mm$, $1.7{\mu}m$) at a column temperature of $40^{\circ}C$ by gradient elution with 0.1% (v/v) formic acid in water and acetonitrile as the mobile phase. The flow rate was 0.3 mL/min and the injection volume was $2.0{\mu}L$. Calibration curves of all compounds were acquired with values of the correlation coefficient ${\geq}0.99$ within the test ranges. The limits of detection and quantification for all analytes were 0.01 - 4.53 ng/mL and 0.03 - 13.60 ng/mL, respectively. The concentrations of the compounds 1 - 9 and 12 were 72.83, 4389.00, 4859.00, 3155.17, 223.67, 33.50, 1.97, 518.00, 2.25, and $25.00{\mu}g/g$, respectively. However, compounds 10 and 11 were not detected.

• 생약학회지
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• 제49권3호
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• pp.278-284
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• 2018

Asimina triloba contains many kinds of annonaceous acetogenins known to have toxicity and/or activity. In this study, analysis of acetogenins was carried out on the extract and its solvent fractions of A. triloba leaves using a liquid chromatography mass spectroscopy(LC-MS). In the extract, the ethanolic extract was significantly higher acetogenin contents than hot water extract. In the analysis of solvent fraction from the ethanolic extract, n-hexane, ethylacetate, and aqueous fraction were contained 85.17~90.92%, 8.96~14.52% and 0.12~0.35% of total acetogenin in the extract. Based on these results, the analytical method is useful for comparing annonaceous acetogenins contents in plant samples. Moreover, when research or product development aiming at the physiological activity of acetogenin such as anticancer activity using A. triloba leaves is performed, a fractionation using a nonpolar organic solvents and alcohol extract may be useful methods. This method can also be used for the purpose of eliminating toxicity as well.

• Molecules and Cells
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• 제22권1호
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• pp.36-43
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• 2006

Mesenchymal stem cells (MSCs) are promising candidates for cell therapy and tissue engineering, but their application has been impeded by lack of knowledge of their core biological properties. In order to identify MSC-specific proteins, the hydrophobic protein fraction was individually prepared from two different umbilical cord blood (UCB)-derived MSC populations; these were then subjected to two-dimensional (2D) gel electrophoresis and peptide mass fingerprinting matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF)-mass spectrometry (MS). Although the 2D gel patterns differed somewhat between the two samples, computer-assisted image analysis identified shared protein spots. 35 spots were reliably identified corresponding to 32 different proteins, many of which were chaperones. Based on their primary sub-cellular locations the proteins could be grouped into 6 categories: extracellular, cell surface, endoplasmic reticular, mitochondrial, cytoplasmic and cytoskeletal proteins. This map of the water-insoluble proteome may provide valuable insights into the biology of the cell surface and other compartments of human MSCs.