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Studies on Takju Brewing with Potatoes (감자를 이용(利用)한 탁주제조(濁酒製造)에 관(關)한 연구(硏究))

  • Kim, S.Y.;Oh, M.J.;Kim, C.J.
    • Applied Biological Chemistry
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    • v.17 no.2
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    • pp.81-92
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    • 1974
  • In order to prepare the mashing materials for 'Takju', Korean wine, with potatoes, theywere steamed, dryed, and pulverized, and their chemical components were analyzed. As a brewing method of Takju with potatoes, general 2nd stage process with Ipkuk and Bunkuk (enzyme sources), commonly used now, was carried out and the effects of preparing conditions of Ipkuk(koji) with potato flour, mashing materials and brewing conditions on the contents of Takju mash, and of storing time on the contents of Takju, were investigated and the results obtained were summarized as follows, 1. Chemical components of steamed potatoes and potato flour were Moisture; 76.2, 10.8%, Total sugar; 16.1, 69.8%, Reducing sugar; 3.45, 13.4%, Crude protein; 2.1, 11.3%, Total acid; 0.012, 0.023% and Volatile acid; 0.0012, 0.0025% respectively. 2. The most effective preparing conditions of Ipkuk with potato flour were to incubate the potato flour added 40-50% of water for 48 hours by general preparing process of Koji, and liquefying and saccharogenic amylase activities of Ipkuk incubated at above conditions were $D40^{\circ}$ 30' 128 W.V. and 13.2 A.U.. 3. The effects of various brewing conditions on the contents of Takju mashes were as follows; 1) Optimum ratio of mashing water and materials for Takju brewing with potato flour was 140ml of water to 60g of flour in 1st stage and 260ml to 140g in 2nd stage. 2) Optimum fermentating times and temperatures for Takju brewing were at $25^{\circ}C$ for 48 hours in 1st stage and at $30^{\circ}C$ for 48 hours in 2nd stage. 3) Optimum amounts of enzyme sources for Takju brewing were 20-30% of Ipkuk and 0.5% of Bunkuk in 1st stage and 1.0% of Bunkuk in 2nd stage. 4) Methanol content of the Takju mash brewed with raw potato flour was much more than that with steamed potato flour. 5) Alcohol, fusel oil and Formol nitrogen contents of the Takju mash brewed with potato flour were less than that with wheat flour, on the contrary, methanol contents and total acidifies of them were showed conversely above. 4. The changes of chemical components and microflora in the mashes during the brewing potato flour Takju were as follows; 1) The accumulation of ethanol followed rapidly in early stage, being the highest at 72 hours (11.9%). 2) Total sugar content of the mash was decreased considerably within 48-72 hours, being 2.62% at 72 hours, and thereafter slowly. 3) Reducing sugar of the mash had a tendency of decreasing, being 0.29% at 48 hours. 4) Total acidity, volatile acidity and Formol nitrogen content of the mash were increased slowly, being 7.30, 0.20, 2.55 at 48 hours. 5) Total cells of yeast appeared the highest in 72 hours ($2.1{\times}10^8$) and thereafter decreased slowly. 6) Total cells of bacteria appeared the highest in 48 hours ($2.4{\times}10^8$) and thereafter decreased or increased slightly. 5. Takju was made from the fermented mash mixed with water to be 6% of alcohol content, and the change of alcohol content, total acidity, total cells of yeast and bacteria during the storing at $30^{\circ}C$ were as follows; 1) Alcohol content of Takju was increased slightly at 24 hours (6.2%), and thereafter decreased slowly. 2) Total acidity of Takju was increased gradually, being 6.1 at 72 hours 3) Total cells of yeast and bacteria appeared the highest at 48 hours ($2.3{\times}10^8,\;1.5{\times}10^8$), and thereafter decreased slowly. 6. Alcohol content, total acidity and Formol nitrogen content of the Takju brewed with potato flour Ipkuk or wheat flour Ipkuk and steamed potatoes(1:5) were 9.8-11.3%, 5.8-7.4, 2.5-3.3 respectively, and the color of the Takju. was similar to commercial Takju. 7. The results of sensory test for various experimental Takju, showed that the Takjues brewed with the materials combined with wheat flour and steamed potatoes(4:5 or 3.5:7.5) were not significantly different in color, taste and flavor from commercial Takju, However, those with potato flour and wheat flour (1:1 or 7:3) were significantly different from commercial Takju.

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Studies on Takju Brewing with Potatoes (감자를 이용(利用)한 탁주제조(濁酒製造)에 관(關)한 연구(硏究))

  • Kim, S.Y.;Oh, M.J.;Kim, C.J.
    • Korean Journal of Agricultural Science
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    • v.1 no.1
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    • pp.67-81
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    • 1974
  • In order to prepare the mashing materials for "Takju", Korean wine, with potatoes they were steamed, dryed, and pulverized, and their chemical components were analyzed. As a brewing method of Takju with potatoes, general 2nd stage process with Ipkuk and Bunkuk (enzyme sources), commonly used now, was carried out and the effects of preparing conditions of Ipkuk(koji) with potato flour, mashing materials and brewing conditions on the contents of Takju mash and of storing time on the contents of Takju, were investigated and the results obtained were summarized as follows. 1. Chemical components of steamed potatoes and potato flour were Moisture; 76.2, 10.8%, Total sugar; 16.1, 69.8%, Reducing sugar; 3.45, 13.4%, Crude protein; 2.1, 11.3%, Total acid; 0.012, 0.023% and Volatile acid; 0.0012, 0.0025% respectively 2. The most effective preparing conditions of Ipkuk with potato flour were to incubate the potato flour added 40-50% of water for 48 hours by general preparing process of Koji, and liquefying and saccharogenic amylase activities of Ipkuk incubated at above conditions were $D_{40^{\circ}}{^{30{\prime}}}$ 128 W.V. and 13.2 A. U. 3. The effects of various brewing conditions on the contents of Takju mashes wereas follows; 1) Optimum ratio of mashing water and materials for Takju brewing with potato flour was 140ml of water to 60g of flour in 1st stage and 260ml to 140g in 2nd stage. 2) Optimum fermentating times and temperatures for Takju brewing were at $25^{\circ}C$ for 48 hours in 1st stage and at $30^{\circ}C$ for 48 hours in 2nd stage. 3) Optimum amounts of enzyme sources for Takju brewing 20-30% of Ipkuk and 0.5% of Bunkuk in 1st stage and 1.0% of Bunkuk in 2nd stage. 4) Methanol content of the Takju mash brewed with raw potato flour was much more than that with steamed potato flour. 5) Alcohol fusel oil and Formal nitrogen contents of the Takju mash brewed with potato flour were less than that with wheat flour, on the contrary, methanol contents and total acidities of them were showed conversely above. 4. The changes of chemical components and microflora in the mashes during the brewing potato flour Takju were as follows; 1) The accumulation of ethanol followed rapidly in early stage, being the highest at 72 hours (11.9%) 2) Total sugar content of the mash was decreased considerably within 48-72 hours, being 2.62% at 72 hours, and thereafter slowly. 3) Reducing sugar of the mash had a tendency of decreasing, being 0.29% at 48 hours. 4) Total acidity, volatile acidity and Formal nitrogen content of the mash were increased slowly, being 7.30, 0.20, 2.55 at 48 hours. 5) Total cells of yeast appeared the highest in 72 hours ($2.1{\times}10^8$) and thereafter decreased slowly. 6) Total cells of bacteria appeared the highest in 48 hours ($2.4{\times}10^8$) and thereafter decreased or increased slightly. 5. Takju was made from the fermented mash mixed with water to be 6% of alcohol content, and the change of alcohol content, total acidity, total cells of yeast and bateria during the storing at $30^{\circ}C$ were as follows; 1) Alcohol content of Takju was increased slightly at 24 hours (6.2%), and thereafter decreased slowly. 2) Total acidity of Takju was increased gradually, being 6.1 at 72 hours 3) Total cells of yeast and bacteria appeared the highest at 48 hours ($2.3{\times}10^8$, $1.5{\times}10^8$) and thereafter decreased slowly. 6. Alcohol content, total acidity and Formol nitrogen content of the Takju brewed with potato flour Ipkuk or wheat flour Ipkuk and steamed potatoes(1:5) were 9.8-11.3%, 5.8-7.4, 2.5-3.3 respectively, and the color of the Takju was similar to commercial Takju. 7. The results of sensory test for various experimental Takju, showed that the Takjues brewed with the materials combined with wheat flour and steamed potatoes (4:5 or 3.5:7.5) were not significantly different in color, taste and flavor from commercial Takju, However, those with potato flour and wheat flour (1:1 or 7:3) were significantly different from commercial Takju.

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Evaluation of Soil and Fertilizer Management Techniques Applied by Farmers in Forcing and Semi-forcing Cucumber Cultivation Facilities (오이 촉성재배와 반촉성재배 농가들의 토양 및 시비관리기술 평가)

  • Lee, Ju-Young;Jang, Byoung-Choon;Sung, Jwa-Kyung;Lee, Su-Yeon;Kim, Rog-Young;Lee, Ye-Jin;Park, Yang-Ho;Kang, Sung-Soo;Hyun, Byung-Keun
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.6
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    • pp.983-991
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    • 2012
  • These days, agricultural products cultivated in facilities occupy the highest percentage of agricultural output price. Specifically cucumbers have been one of the crops that farmers prefer to growing, because their prices were high. However, cucumber crop is sensitive to the soil and environments and it requires the exact crop management. In order to establish cultivation techniques for cucumbers, the current situation of cucumber cultivation was surveyed from ten cucumber farmlands; five farmlands of cucumber cultivation in forcing and five of semi-forcing practicing systems, respectably. The soil conditions were alluvial or valley in soil topology, moderately or poorly drainage in soil drainage classes, coarse loamy in soil texture family. Soil was managed with deep plowing combined with application of basal fertilizers such as compost, rice straw, oil cake, wood chip and chemical fertilizer. The whole soil was prepared in uniformly with rotary. Three major nutrients ($N-P_2O_5-K_2O$)of basal application were 815-464-529 kg $ha^{-1}$ in forcing and 197-135-151 kg $ha^{-1}$ in semi-forcing cultivation. Top dressing of fertilizer was supplied in fertigation system of macro and micro elements in 2~3 day interval with water irrigation. The average yields of cucumbers were $168t\;ha^{-1}$ with 381,000 thousand won $ha^{-1}$ in average gross profit (AGP) in forcing cultivation and $115t\;ha^{-1}$ with 177,000 thousand won $ha^{-1}$ in AGP in semi-forcing cultivation. Cucumber production during the winter season was considered to increase the gross profit because cucumber price tends to stay in high level during this time. The accumulation of soil chemicals like EC, available $P_2O_5$ and exchangeable cations could be controlled by rice straw application. The rice straw application increased soil temperature during the winter season, in exchange of soil air, and in extension of plant roots. In addition, the rice straw application somewhat affected decrease of salts accumulation.

Effects of Supplementary Herbs and Plant Extracts on the Performance of Broiler Chicks (생약제(Herbs, Plant Extracts)의 급여가 육계의 생산성에 미치는 영향)

  • Woo, K.C.;Kim, C.H.;NamGung, Y.;Paik, I.K.
    • Korean Journal of Poultry Science
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    • v.34 no.1
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    • pp.43-52
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    • 2007
  • Experiments were conducted to investigate the effects of dietary botanicals (herbs and plant extracts) on the performance, nutrient metabolizability, small intestinal microflora, IgG level and blood parameters in broiler chickens. In Exp. 1, 1,000 (500 each sex) broiler chicks($Ross^{(R)}$) were divided into 20 groups of 50 chickens each(25 birds each sex). Four groups were assigned to each of five dietary treatments:control and diets containing antibiotics($Avillamix^{(R)}$, avillamycin-premix), Herb M(Herb $mix^{(R)}$), Plant extract B(BIOSTRONG $510^{(R)}$) and Plant extract A($APEX^{(R)}$). In Exp. 2, 240(120 each sex) broiler chicks($Ross^{(R)}$) were devided into six treatment groups:control and diets containing antibiotics($Avillamix^{(R)}$, avillamycin-premix), Plant extract D($Digestarom^{(R)}$), Plant extract P($Phellozyme^{(R)}$), Plant extract G($Galicin^{(R)}$) and Plant extract C(CRINA $POULTRY^{(R)}$). Each treatment consisted of four replicates of 10 birds each. In both experiments, birds had free access to diets and water for 5 wk on floor pens(Exp. 1) and cages(Exp. 2). In Exp.1, production index of groups fed diets supplemented with herbs and plant extracts was slightly higher than the control and those fed Herb M was highest. In Exp. 2, groups fed diets supplemented with herbs and plant extracts consumed more feed than the control during the period between 4 and 5 wk(P<0.05). Feed conversion(feed/gain) was lower in antibiotics group than other groups. The values of RBC, Hb and HCT were higher(P<0.05) in chicken fed diets supplemented with the additives than in the control in Exp. 1. BA value was lower(P<0.05) in groups fed diets supplemented with the additives than in the control in Exp. 2. Serum IgG were higher(P<0.05) in groups fed diets supplemented with the additives than in the control in both experiments. The cfu of intestinal microflora and metabolizability of nutrients were not significantly different among treatments in both experiments. It was concluded that the botanical supplements can be used as an alternative to antibiotics in broiler diets.

Morphology of Larvae, Egg Development and Spawning Behavior of the Blenniid Fish, Pictiblennius yatabei (Jordan et Snyder) (청베도라치, Pictiblennius yatabei의 산란습성(産卵習性), 난발생과정(卵發生過程)및 부화자어(孵化仔魚)의 형태(形態))

  • Kim, Yong-Uk;Myoung, Jung-Goo;Han, Kyeong-Ho;Kang, Chung-Bae
    • Korean Journal of Ichthyology
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    • v.4 no.2
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    • pp.44-54
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    • 1992
  • The blenniid fish, Pictiblennius yatabei(Jordan et Snyder) is ditributed in the central and southern Japan and southern Korea. The fish grows to a maximum size of 9cm in total length, and it seems to be matured in two years. Sex dimorphisms of the fish appear in the crest and the anal fin. Five egg masses of the fish were collected in Tongyoung Bay near Kyeong Nam on June 16 and 20, 1991. The eggs were laid on inner surface of empty shells of the oyster, Crassotrea gigas and blue mussel, Mytilus edulis. Each egg mass was guarded by the male parent. Numbers of eggs in each of masses were as about 450~1000.The eggs were spherical but somewhat oval in shape and ranged from 0.72 to 0.80mm in longer axis and from 0.55 to 0.65mm in shorter axis. Each egg was provided with an adhesive pedestal. Hatching took place in 105 hours after formation of embyo at the water temperature varying from 20.0 to $21.4^{\circ}C$. Melanophores appearing on the yolk remarkably changed in form during the embryonic development.The newly hatched larvae, having 32~34 somites, were from 2.71 to 3.35mm in total length. The larvae absorbed the yolk material and oil globule completely in 7 days after hatching and became postlarvae. Nine days after hatching, the larvae averaged 5.35mm in TL and the caudal notochord flex at $45^{\circ}$ Total lengths of the larvae reached 6.00 and 6.58mm in 11 and 13 days after the hatching, respectively.

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The Influence of Food Habits on Body Stature of Children (어린이의 식습관(食習慣)이 체위(體位)에 미치는 영향(影響)에 관한 연구(硏究))

  • Lee, Mi-Suk;Mo, Su-Mi
    • Journal of Nutrition and Health
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    • v.9 no.1
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    • pp.7-15
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    • 1976
  • The Purpose of this study was to determine every possible correlation between food habits and body statures of primary school children, aged ten years old. This study was conducted from July to October of 1975. In July, prepared questionaires concerning life style, anthropometry, food preference, and food behavior were distributed through school teachers to 425 children; 219 boys and 206 girls, in the 5th grade of three elementary schools in the city of Seoul. Then, in October, when subjects had been classified into underweight/obese by statistical analysis, mothers of obese or underweight children were interviewed by the authors to determine weaning history, daily food consumption of their children, and opinions of various snacks for children. Analysis of results in terms of correlation coefficient, chisquare test and percentage calculations, are as follows: 1. Physical growth and development Boys Girls Height (cm) $134.8{\pm}5.74\;134.4{\pm}5.97$ Weight (kg) $30.0{\pm}4.27\;29.5{\pm}5.16$ Chestgirth (cm) $64.1{\pm}3.59\;63.3{\pm}3.81$ Arm circumference (cm) $18.3{\pm}1.61\;18.2{\pm}1.70$ Triceps skinfold thickness (mm) $10.9{\pm}5.13\;12.7{\pm}4.86$ Various indices of nutrition such as relative weight, relative chestgirth, $R{\ddot{o}}hrer's$ index, Kaup index, Vervaeck index were determined. 2. Food habits 1) Food $preference{\cdots}{\cdots}A$ varying number of foods were selected from 60 items were accepted. It was found that the food which children liked best was fruit and snacks were popular one. Lowest ranking among LIKED foods were from strongly flavored vegetables and organ meat. In general, girls had more food dislikes than did boys. Selected as liked foods were fruits, rice noodle soup, biscuits, and peanuts. Disliked foods were liver, green onions, onions, green pepper, mushrooms, oysters, shellfish, and pork. Items which children never ate before were liver, mushrooms, fish cake, boiled rice mixed with sorghum, mayonnaise, and fresh water firsh. Reasons which children gave for dislike were undesirable flavor and odor. 2) Food $behavior{\cdots}{\cdots}It$ was found that boys liked sweet and salty flavors more than did the girls who more often liked sour flavor. The majority of children enjoyed evening meals more than lunch and breakfast. A number of children skipped breakfast because of lack of appetite or lack of time before going to school. Snacks were the most popular food, especially among girls. Their snacking habits were formed by the encouragement of the mother, and the relieve boredom. Educational backgrounds of mothers and family economical levels of children were remarkable correlated with mothers' attitudes toward feeding of children. There were several interesting findings relating body stature to some other responses; such as that the obese child has a small number of brothers, higher birth order, higher educated mothers and higher family cultural background. It was also discovered that food perference, except for fat and oil group foods was not related to body stature. Sweet taste was liked best and pepperly taste was mostly disliked. Sour taste was popular in the group of underweight. Underweight children were more finicky, disliked snacking, and didn't get much attention from their mothers. 3) Correlation between body stature and nutrition during their infancy. The majority of children, both from obese and underweight, were breast fed as infant. Twenty five per cent of obese children and 17.4 per cent of underwight children started weaning at $1{\sim}6$ months old. The most popular supplemental food of weaning was cereal gruel for the obese group, while boiled white rice and cereal gruel were most common for the underweight group. Highly significant relationships were found between stature of parents and their children. In the obese group 47.8 per cent of fathers and 45.9 per cent of mothers were overweight; however, none of the fathers and only one mother was found to be underweight. In daily food consumption patterns, meals consisting of bread or noodle were popular in the obese group but disliked by the underweight group. The study found clear contrast in that the obese group liked meat and fish while the underweight group liked fruits and vegetables, especially kimchee. The obese children desired to eat cereal foods, milk and milk products, and fat foods while the underweight group desired to eat fruits and vegetables. Frequency of snacks per day was much greater in the obese group. Foods which mothers believed to be good for the health were carrots, cucumbers, fruits, milk, potatoes, sweet potatoes, and breads, while sweet foods such as candies, drinks. chocolate were considered not good for the teeth. Watching television was not significantly related to statures of children. Most significant relationships were found beween frequencies of family guest meals/and eating-out, and children's statures. Nutritional problems which have been considered for the malnourished children in addition to those of children who have a tendency toward obesity, must be taken into consideration in the development of proper nutrition education through the channels of regular school teaching and teaching by parents in the homes. Korean standards of anthropometric measurements for children should be revised, current measurements show much higher figures than present Korean standards.

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A STUDY ON THE FOOD OF THE GOBY, SYNECHOGOBIUS HASTA (풀망둑 Synechogobius hasta (TEMMINCK et SCHLEGEL)의 먹이 조사)

  • PAIK Eui-In
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.2 no.1
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    • pp.47-62
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    • 1969
  • A goby, Synechogobius hasta (Temminck et Schlegel) was studied to investigate the food consumed and the biological change of the food organisms, and the fish were sampled from the closed tributary and the lower Part of the Naktong River, near Pusan, during the period from November of 1967 to December of 1968. The fish were sampled from four stations (Fig. 1), the total number of fish being 1,295 and they were grouped and analysed monthly. The content of the alimentary canal was analysed in three categories according to modified Nilsson's method (Dahl 1962) with a slight alteration: 1) The number of each item of stomach contents was counted and the percentage of each item in proportion to the total number of food organisms is indicated by the letter 'N' representing numerical percentage in Table 2. 2) The percentage of fish which contained any items of food organisms in proportion to the total number of fish caught in a given season is indicated by the letter 'O' representing frequency of occurrence. 3) Dominant groups of food items were selected and the percentage of the number of each dominant item in proportion to the number of the food organisms belonging to the dominant groups is indicated by the letter 'D' representing dominance. All food organisms were classified in 50 food item categories and then they were grouped in 13 main groups (Fig. 2-1), and they were further divided into 1) obligatory bottom animals, 2) organic drifts and 3) actively swimming forms; according to the conditions of the animal communities within the habitat. Since the majority of its food was composed of the obligatory bottom animals ($94.6\%$), the fish appeard to be a typical bottom feeder. And the dominant food organisms of the fish is generally determined by the local composition of the benthic fauna within the fish habitat. And their seasonal rhythm occurs among the food organisms in the stomach by the biological interaction. Locality variation in the population of the same food organism occurs due to the difference of food organisms in the habitat of the fish at Seonam and Garak, and at Seongsan and Hadan the condition of the niche for the fish in the both regions seems to be the same since the composition and the seasonal variation of the organisms were the same. The results may be summarized as follows: 1) The goby mainly feed on the animals of bottom fauna, and the food organisms are deter-mined by the food compositions within the habitat. 2) Seasonal variation of the stomach content shows the seasonal rhythm due to the biological variation of the population and their interaction. 3) The goby shows no preference on specific food, and the food is composed of a variety of animals. 4) Major food items of the goby are Polychaeta, Palaemon modestus, Isopoda, Gammaridea, Insecta (nymphs and larvae), Ilyoplax deschampsi, and Paratye compressa. 5) Logitudinal succession oil the population of the food organisms is apparently recognized within the community of Seongsan, Garak and Seonam. 6) The goby begins to descend toward the estuary and sea around April when the water temperature reaches $20^{\circ}C$, and they begin to return to river waters in September.

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Avifauna and Management of Breeding Season in Taeanhaean National Park (태안해안국립공원의 번식기 조류상과 관리)

  • Paik, In-Hwan;Jin, Seon-Deok;Yu, Jae-Pyoung;Paek, Woon-Kee
    • Korean Journal of Environment and Ecology
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    • v.24 no.2
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    • pp.139-146
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    • 2010
  • The survey was done in order to find what kinds of birds visit Taeanhaean National Park during breeding season, where we fixed up 10 coastal areas and islands within the National Park. Three groups concurrently performed the field research from 5th to 9th of July in 2009. Total 58 species and 7,323 individuals were recorded in Taeanhaean National Park. 48 species including 6,187 individuals were observed in coastal areas and 33 species including 1,136 individuals in island areas. The most dominant species in the National Park are Larus crassirostris which accounts for 60% of the birds inhabiting there, and they seem to have been bred in the islands near the National Park. The birds observed only around the coastal areas include Anas poecilorhyncha, Fulica atra, Egretta intermedia and the others which consist of 25 species and amount to 318 individuals, and the birds found exclusively in island areas include Phalacrocorax filamentosus, Apus pacificus¸ Locustella pleskei and other birds, which consist of 10 species and the number of those individuals observed was 308. The inhabited islands areas such as Gauido were characterized by high ratio of waterbird population, which seems to be correlated with the factors such as the extent of island, the richness of water resources, and the diversity of habitats. Based on the data collected during the research and other data from the previous observations, the kinds of dominant species remain nearly unchanged. And in spite of the oil spill accident in 2007, the increase in the number of waterbirds compared to 2004 may be the evidence that the area is recovering from the environmental pollution. At present, the tidal power plants are being built or scheduled to be built and large-scale reclamation is also under way. What is worse, those areas are seeing the increase of pension construction, which is likely to be the potential cause of damage and disturbance against some key habitats for the waterbirds. Therefore, it is a major priority that we build the bird information system to efficiently manage the knowledge-based asset collected from bird-watching groups and to better monitor the areas that need enhanced database through which the National Park can be appropriately administered.

Reproductive Cycle of Small Filefish, Rudarius ercodes (그물코쥐치, Rudarius ercodes의 생식주기)

  • LEE Taek Yuil;HANYU Isao
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.5
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    • pp.423-435
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    • 1984
  • The reproductive cycle of the small filefish, Rudarius ercodes was investigated based on the annual variations of gonadosomatic index(GSI) and hepatosomatic index(HSI) by electronic and photic microscophy. The specimens used were collected at the coastal area of Benden island, Sizuokagen, Japan, from September 1982 to August 1983. GSI began to increase from March, starting season of longer daylength and higher water temperature, and reached the maximum value between June and August. It began to decrease from September with the lowest value appearing between November and February without any evident variation. The annual variations of HSI were not distinct in male filefish and were negatively related to GSI in female : HSI decreased in the summer season when the ovary was getting mature and reached the maximum in the winter season when the ovary was getting retrogressive. The ovary consisted of a pair of saccular structure with numerous ovarian sacs branched toward the median cavity. Oogonia divided and proliferated along the germinal epithelium of the ovarian sac. Young oocytes with basophile cytoplasm showed several scattering nucleoli along the nuclear membrane. when the oocytes growing to about 300 ${\mu}m$, nuclear membrane to disappear with nucleus migrating toward the animal pole. The regions of protoplasm were extremely confined within the animal hemisphere in which most of cytoplasms were filled with yolk materials and oil drops. After ovulation, residual follicles and growing oocytes remaining in the ovarian sacs degenerated. But perinucleatic young oocytes without follicles formed were not degenerated, and growing continuously still in the next year. Mitochondria and endoplasmic reticula in the cytoplasm remarkably increased with oocytes maturing and yolk accumulating. Those were considered to be functionally related to the yolk accumulation. Five or six layers of possible vitellogenin, oval-shaped disc structures with high electron density, appeared in the apex of follicular processes stretching to the microvilli pits of mature oocytes. Testis consisting of a pair of lobular structures in the right and left were united in the posterior seminal vesicle, Cortex of testis was composed of several seminiferous tubules, and medulla consisting of many sperm ducts connected with tubules. Steroid hormone-secreting cells with numerous endoplasmic reticula and large mitochondria of well developed cristae were recognized in the interstitial cells of the growing testis. Axial filament of spermatozoon invaginated deeply in the central cavity of the nucleus and the head formed U-shape with acrosome severely lacking, mitochondria formed large globular paranuclei at the posterior head, and microtubular axoneme of the tail represented 9+9+2 type. The annual reproductive cycles could be divided into five successive stages : growth(March to July), maturation(May to September), Spawning(mid May to early October) and resting stages(October to February). The spawning peak occurred from June to August.

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Changes of Physical Properties of Soils by Organic Material application (유기성 물질 시용에 따른 농경지 토양물리성 변화 연구)

  • Kim, Lee-Yul;Cho, Hyun-Jun;Han, Kyung-Hwa
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.5
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    • pp.304-314
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    • 2004
  • The objective of this study was to investigate the effect of organic materials (compost, straw, green manure, pig manure, seed production oil cake, and industrial by products including municipal sewage sludge, industrial sewage sludge, leather processing sludge, and alcohol fermentation processing sludge) on physical properties of soils in seven paddy and four upland fields with differential soil textures, sandy loam, loam, or clay loam, etc. The investigated physical parameters were bulk density (BD), air permeability (AP), macroporosity, hardness, shear resistance, frictional resistance, water stability aggregate (WSA), and Middleton's dispersion ratio. Except for coarse sandy loam field with weak structure, a decrease in BD and shear resistance, and an increase in macroporosity and AP in plots with applying organic materials compared to plots without applying organic materials appeared. In upland fields, the positive effect of organic materials on WSA, BD, and air permeability was higher than in paddy fields. The combined plot of NPK and compost had lower BD, hardness, and shear resistance, and higher macroporosity and WSA than plot with compost. Green manure had higher positive effect on physical properties of soils compared to other organic materials and the extent of positive effect had no significant correlation with soil organic matter content. Of industrial byproducts applied in coarse sandy loam soil under upland condition, municipal sewage sludge and pig manure compost had higher effect on increase of WSA than leather processing sludge and alcohol fermentation processing sludge. Unlike WSA, there were no significant differences between industrial byproduct types in other physical properties. in silty clay loam soil under the upland condition, straw had more positive effect on soil physical parameters than hairy vetch and pig manure. Therefore, different organic materials had differently active effect on physical parameters depending on types of soil and land use. Especially, it could be thought that well-decomposed organic materials have the advantage of an increase in organic matter content, while coarse organic materials of an increase in WSA.