• Journal of the korean academy of Pediatric Dentistry
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• v.24 no.1
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• pp.204-219
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• 1997

The use of fluoride is one of the most effective methods for caries prevention. Fluoridation of public water supply has been recognized, for many years, as an effective way to reduce dental caries. The fluoride supplement has been recommended when the natural fluoride was unavailable or below the optimal range. However the mechanism of caries prevention by fluoride has not yet been clarified and it is well known that an overdose of fluoride results inacute and chronic toxicity, especially dental fluorosis. Fluoride mouthrinsing solution is widely used in dentistry due to its effectiveness in carrying anticariogenic action. Understanding the effects of fluoride mouthrinsing solution on human gingival fibroblasts will provide the safety rationale for its use during the caries preventive therapy. The purpose of this study was to evaluate the cytotoxic effect of fluoride mouthrinsing solution on the human gingival fibroblast in vitro. The human gingival fibroblasts were cultured from healthy gingiva on the extracted deciduous teeth of children. Cells were inoculated into a 24-well plate with $1{\times}10^4cells/well$ of medium at $37^{\circ}C$, 100% humidity, 5% $CO_2$ incubator for 24 hours. And the cells were counted by using the hemocytometer at each designed study. Human gingival fibroblasts were cultured in growth medium after one minute application range of 0.02%-0.2% NaF solution and 0.1% $SnF_2$ solution. The cells used in this study were between fifth to eighth passage number. The cell morphology was examined by inverted microscope and cell proliferation was measured by incorporating $[^3H]$-thymidine into DNA. DNA synthesis by human gingival fibroblasts was assessed by $[^3H]$-thymidine uptake assays while the cell activity was measured by MTT assay. Each concentrated fluoride mouthrinsing solution was estimated for its biocompatability with fibroblasts by the tissue culture technique. The results of this study were as follows : 1. It was observed that at 0.05%, 0.2% NaF mouthrinsing solution the cytoplasmic processes became globular. When 0.1% $SnF_2$ mouthrinsing solution was applied, the cytoplasmic process and cell morphology were disappeared. 2. DNA synthetic activity was reduced regardless of the concentration of the fluoride mouthrinsing solution. However, the result is statistically insignificant except 0.1% $SnF_2$ mouthrinsing solution(p<0.05). 3. Our results indicate that 0.02%, 0.05% concentrations of NaF mouthrinsing solution caused minimal cytotoxicity. But 0.2% NaF and 0.1% $SnF_2$ concentration were a significant difference between the cell activity in the experimental group and control group (p<0.05). 4. After appling 0.05% & 0.02% NaF fluoride mouthrinsing solution, cell activity was restored to the control groups level according to incubating time. The results suggest that direct exposure to fluoride solution inhibits gingival fibroblast activity. Therefore, for the most effective use of fluoride use, lowering the concentration of fluoride mouthrinsing is advisable because it maintains biocompatability and free ion in the oral fluid.

• Journal of Korean society of Dental Hygiene
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• v.1 no.2
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• pp.201-211
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• 2001

Dental caries causes the majority of tooth loss among Koreans. Korea is experiencing an increase in dental caries. And it shows a high prevalence of caries in preschool children, which contrasted with the much-improved situation in developed countries. So, it is important to control caries from preschool periods in Korea. Recently, inspections of the teeth of preschool children for evidence of dental disease became a legislative duty in Korea following Oral Health Act(2000). But, standardized oral health programs in preschool children is not yet proposed. Therefore, it is necessary to develop an community based oral health program in preschool children. The purpose of this study was to obtain basic data for the development of community based oral health program in preschool children of Sungnam city. Oral health status in primary dentition were surveyed in 862 2-6 year-old preschool children of Sungnam city by WHO criteria. From the data, df rate, percentage of preschool child ren with active caries, dft index, dt rate, percentage of preschool children with pit and fissure sealant of each age were calculated, and discussed. The obtained results were as follows : 1. The experience of dental caries in primary dentition showed a tendency to increase quickly between 2 and 3 year old children. So, the oral health program in preschool children should be developed to prevent dental caries and control their oral health under three years of age. 2. In 5 year-old children, the percentage with active caries was 60.8% and dft index was 5.06. The dt rate showed a tendency to decrease as proportion to age, but even though 6 year-old children, the dt rate was 47.7%. Therefore, the annual screening dental examination and oral health education programs at the institution for preschool children should be developed to detect and treat dental caries in primary dentition at early stage. 3. The percentage of preschool children with pit and fissure sealant at six years of age was 10.2%. So, pit and fissure sealant and professional fluoride application programs in Public Health Center should be developed to prevent dental caries. 4. Also, it is necessary to establish oral health goals in preschool children and develop water fluoridation program 10 improve and preserve oral health of preschool children in Seongnam city, effectively.

• Journal of the korean academy of Pediatric Dentistry
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• v.26 no.2
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• pp.262-274
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• 1999

Several alternatives for increasing the fluoride concentration in the mouth, such as water fluoridation, ingestion of fluoride supplements, fluoride paste, fluoride mouthrinse, application of fluoride gel are available. There is an impressive body of evidence that the topically deliverd fluorides are clinically effective in inhibiting the progression of dental caries. Recent studies on the cariostatic action of fluoride have indicated the importance of fluoride in the fluid environment of the teeth. The fluoride levels in unstimulated whole saliva can be considered indicative of F in the aqueous phase available for interaction with the tooth surface at a given time. The retention of F in the mouth after topical fluoride treatment is considered to be an important factor in the clinical efficacy of F. The aim of this study was to determine the elevation and clearance of fluoride in whole saliv after the following topical flouride treatments using HMDS-diffusion technique and fluoride ion electrode. The obtained results were as follow: 1. Average salivary fluoride concentration in the unstimulated whole saliva was $0.0152ppm{\pm}0.0091ppm$. Unstimulated salivary flow rate was between 0.34-0.36ml/min and there was no statistically significant difference among the groups(p>0.05). 2. Except for the immediate time after treatment, fluoride levels followed as APF gel>neutral gel>F-rinse>F-paste. There was no statistical difference between the salivary F concentration of F-paste group and that of control group after 2 hours. In case of F-rinse group, after 3 hours the concentration had dropped to baseline value. But there was statistically significant difference among the F concentraion of F gel groups and that of control group(p<0.05). 3. The mean $AUC_{0-120min}$ values were followed as neutral gel>APF gel>F-rinse>F-paste, and the values of the two former groups were significantly higher than those of the two latter groups(p<0.05).

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.1
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• pp.30-37
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• 2009

Early enamel caries is commonly remineralized by the patient‘s improved oral hygiene or fluoridation, however the result is clinically unreliable. As an alternative, we tried to seal the lesions with low-viscosity light-curing resin. The aim of the present study was to search the proper methods of the adequate pretreatment prior to applying adhesive resin on natural proximal caries lesions. Thirty nine extracted deciduous molar teeth showing proximal early caries lesion were used for this study. They were divided into 5 groups : Group 1; only carefully cleaned with water, group 2; etched with 15% HCl for 15s, group 3; etched with 35% phosphoric acid for 15s, group 4; etched with 35% phosphoric acid for 30s, and group 5; cleaned with 0.5% NaOCl. Following results were obtained by evaluating with SEM and CLSM after applied with adhesive resin. 1. As a result of SEM evaluation, group 2 showed clearly removed surface layer, group 3,4 showed partially removed surface layer irregularly, group 5 showed slightly removed surface layer. 2. Group 2 showed the deepest infiltration depth, followed by group 4, group 3, group 5, group 1 and besides group 5, other groups showed significantly deep infiltration depth. (p < 0.01) In conclusion, the best methods of the adequate pretreatment on natural proximal caries lesion for deep infiltration of adhesive resin was to etch with 15% HCl for 15s.

• Journal of dental hygiene science
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• v.3 no.1
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• pp.45-50
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• 2003

In order to develop the program of oral hygiene education for elementary school senior-students, a questionnaire survey of elementary fourth, fifth and sixth students who are under continual control program of oral hygiene. (1) For tooth brushing time, after dinner is more than before. 73.7% of respondents brushed the teeth twice or more a day. (2) 62.1% of respondents took the cariogenic food twice or more a day. (3) 90.1% of respondents had visited a dental clinic. (4) Concerning fear for dental treatment, only 14.4% had a feeling of fear. (5) Regarding the experience with a preventive treatment, 39.7% had an experience with pit and fissure sealing, and 24.2% had an experience with the application of fluorides. (6) 46.2% of respondents had a preference for the fluoride mouth rinsing program, and 38.4% wanted that program to keep going on. As to the reason to favor the fluoride mouth rinsing program, 38.2% preferred it because of prevention of dental caries, and 43.0%, the largest percentage, didn't favor it because they found it too much trouble to do. (7) Comparing with the research result in 2000, we proved positively the educational effect of brushing frequency, positive understanding and preference to water school fluoridation program, and got the negative data of experience of visiting to oral clinic and preventive treatment.