• Membrane and Water Treatment
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• v.10 no.6
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• pp.395-404
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• 2019

The influence of chlorine on marine bacterial communities was examined in this study. A non-chlorine-adapted marine bacterial community (NCAM) and a chlorine-adapted bacterial community (CAM, bacterial community treated with $0.2mg-Cl_2/L$ chlorine) were cultivated for 1 month. A distinct difference was observed between the NCAM and CAM, which shared only eight operational taxonomic units (OTUs), corresponding to 13.1% of the total number of identified OTUs. This result suggested that chlorine was responsible for the changes in the marine bacterial communities. Kordiimonas aquimaris was found to be a chlorine-resistant marine bacterium. The effect of intermittent chlorination on the two marine biofilm communities formed on the reverse osmosis (RO) membrane surface was investigated using various chlorine concentrations (0, 0.2, 0.4, 0.6 and 0.8 mg $Cl_2/L$). Although the average number of adherent marine bacteria on the RO membrane over a period of 7 weeks decreased with increasing chlorine concentration, disinfection efficiencies showed substantial fluctuations throughout the experiment. This is due to chlorine depletion that occurs during intermittent chlorination. These results suggest that intermittent chlorination is not an effective disinfection strategy to control biofilm formation.

• Journal of Life Science
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• v.24 no.5
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• pp.522-531
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• 2014

Seasonal variations in the bacterial community of Gwangyang Bay seawater were analyzed using both isolation and cultivation-independent methods. Amplified rDNA restriction analysis was applied to 200 bacterial isolates. Bacterial isolates were composed of four phyla: Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes. Pyrosequencing was conducted, in addition to denaturing gradient gel electrophoresis (DGGE) of genomic DNA extracted directly from the water samples. The bacterial sequences obtained by pyrosequencing of 16S rRNA genes consisted of 24 phyla in the spring and summer, 39 in the fall, and 32 in the winter. The diversity index was high in the fall, whereas the dominancy index was high in the spring. In the spring, phylum Firmicutes was dominant, whereas phylum Proteobacteria dominated in the other three seasons. The second most dominant phyla were Proteobacteria in the spring, Firmicutes in the summer, and Bacteroidetes both in the fall and winter. Bacilliaceae was the most predominant family in the spring. Rhodobacteraceae and Bacilliaceae dominated in the summer, and Rhodobacteraceae dominated in the winter. Neither was dominant in the fall Twenty-seven bands purified from DGGE profiles were cloned and analyzed phylogenetically. In the spring, phylum Firmicutes dominated, followed by Proteobacteria. Proteobacteria dominated in all other seasons. Thus, two cultivation-independent methods for determination of seasonal variation patterns at the phylum level were in accordance with each other.

• Journal of the Korean Association of Geographic Information Studies
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• v.6 no.3
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• pp.117-128
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• 2003

The purpose of this study is to analyze the characteristics of the landscape type on Chunchon by CG(computer graphics) pictures and visibility analysis. The land use CG picture and the land cover CG picture are created by using Zoning area data and DEM(digital elevation model), and by using data of land cover classification and DEM. According to the analysis result of the land cover from 1989 to 2000, the city area has increased to $7.7km^2$, the green area has diminished to $12.7km^2$. The tendency of the city area increases and the green area decreases which appear in the city area, developmental restriction zone and green area on land use. The landscape is classified into three types by cluster analysis using the area rate of the element which constitutes the land use CG picture. Type 1 is a landscape characteristics of developmental restriction zone. Type 2 is a landscape characteristics of green land and type 3 is a landscape characteristics based on city area and water area. The increase of city area and decrease of green area are shown in all landscape types of land cover CG pictures. The same tendency is seen in the place where the scenery is of high importance as a result of visible analysis. The preservation and management of the scenery to the green area are requird in developmental restriction zone are required.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.2
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• pp.217-223
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• 2019

Objective: An experiment was conducted to investigate the effects of feed intake restriction during late pregnancy on the function, anti-oxidation capability and acute phase protein synthesis of ovine liver. Methods: Eighteen time-mated ewes with singleton fetuses were allocated to three groups: restricted group 1 (RG1, 0.18 MJ ME/kg $W^{0.75}$ d, n = 6), restricted group 2 (RG2, 0.33 MJ ME/kg $W^{0.75}$ d), n = 6) and a control group (CG, ad libitum, 0.67 MJ ME/kg $W^{0.75}$ d, n = 6). The feed restriction period was from 90 days to 140 days of pregnancy. Results: The ewe's body weight, liver weights, water, and protein content of liver in the restricted groups were reduced compared with the CG group (p<0.05), but the liver fat contents in the RG1 group were higher than those of the CG group (p<0.05). The increased hepatic collagen fibers and reticular fibers were observed in the restricted groups with the reduction of energy intake. The concentrations of nonesterified free fatty acids in the RG1 and RG2 groups were higher than those of the CG group with the reduction of energy intake (p<0.05), but there were decreased concentrations of lipoprotein lipase and hepatic lipase in both restricted groups compared with the CG group (p<0.05). In addition, the increased concentrations of ${\beta}$-hydroxybutyric acid, triglycerides, malondialdehyde, total antioxidant capacity and activities of superoxide dismutase activity and catalase were found in the RG1 group, and the concentrations of cholinesterase in the RG1 group were reduced compared with the CG group (p<0.05). For the concentrations of acute phase proteins, the C-reactive protein (CRP) in the RG1 group were reduced compared with the CG group, but there were no differences in haptoglobin relative to the controls (p>0.05). Conclusion: The fat accumulation, increased hepatic fibrosis, antioxidant imbalance and modified synthesis of acute phase proteins were induced in ewe's liver by maternal malnutrition during late pregnancy, which were detrimental for liver function to accommodate pregnancy.

• Proceedings of the Korea Concrete Institute Conference
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• 1999.10a
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• pp.345-348
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• 1999

Since the cement-water reaction is exothermic by nature, the temperature rise within a large concrete mass. Significant tensile stresses may develop from the volumn change associated with the increase and decrease of the temperature with the mass concrete. These thermal stresses will cause temperature-related cracking in mass concrete structures. These typical type of mass concrete include mat foundation, bridge piers, thich walls, box type walls, tunnel linings, etc. Crack control methods can be considered at such stages as designing, selecting the materials, and detailing the construction method. In this paper, the effect of placing of crack control joint or construction joint was analysed by a three dimensional finite element method. As a result, using this method, crack control can be easily performed for structures such as wall-type structures.

• Proceedings of the Korea Concrete Institute Conference
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• 1999.10a
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• pp.333-336
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• 1999

The crack of concrete induced by the heat of hydration is a serious problem, particularly in concrete structures such as mat-slab of nuclear reactor buildings, dams or large footings, foundations of high rise buildings, etc.. As a result of the temperature rise and restriction condition of foundation, the thermal stress which may induce the cracks can occur. Therefore the various techniques of the thermal stress control in massive concrete have been widely used. One of them is prediction of the thermal stress, besides low-heat cement which mitigates the temperature rise, pre-cooling which lowers the initial temperature of fresh concrete with ice flake, pipe cooling which cools the temperature of concrete with flowing water, design change which considers steel bar reinforcement, operation control and so on. The Aim of this paper is to verify the effect of low heat blended cement in reducing thermal stress in slab type's mass concrete such as container harbor structures.

• Journal of environmental and Sanitary engineering
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• v.14 no.4
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• pp.99-109
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• 1999

Enteropathogenic Yersina enterocolitica is an important cause of human and animal disease. Phenotypic and genotypic characteristics currently used to identify Yersinia enterocolitica are not necessarily sufficient to differentiate pathogenic from non-pathogenic strains or to analyze the epidemiology of yersiniae at a molecular level. To improve the characterization of Yersinia enterocolitica, A total of 65 isolates of Yersinia enterocolitica were examined with bioserotyping, antibiotic susceptibilities, PFGE, PCR-ribotyping. Genomic DNA pattern generated by PFGE are highly specific for different strains of an organism and have significant value in epidemiologic investigations. The PFGE analysis of Not I-digested chromosomal DNA of Y. enterocolitica were performed with a CHEF Mapper(Bio-Rad, USA). Not I generated 19 restriction endonuclease digestion profiles(REDP). PCR-ribotyping, performed with primers complementry to conserved regions of 16S and 23S rRNA gene, generated 13 ribotypes. PCR-ribotyping can be considered a good technich for subtyping strains of Y.enterocolitica.

• Journal of ILASS-Korea
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• v.15 no.3
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• pp.109-114
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• 2010

The implementation of gelled propellants systems offers high performance, energy management of liquid propulsion, storability, and high density impulse of solid propulsion. The present study focused on the macroscopic spray characteristics of liquid sheets formed by triplet impinging jets of non-Newtonian liquids which are mixed by Carbopol 941 0.5%wt. The results are compared to experiments conducted on spray images which formed by triplet impinging jets concerning with airassist effect at center orifice. When gel propellants are injected by doublet impinging jets at low pressure and high pressure, closed rim pattern shape appeared by polymeric effect from molecular force and showed inactive atomization characteristics, because of extensional viscosity related by restriction of atomization process and breakup time delay of turbulence transition. As increasing mass flow rate of the air(increasing GAR), spray breakup level is also increased.

• Journal of Ship and Ocean Technology
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• v.1 no.2
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• pp.1-10
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• 1997

The prediction of the total resistance of a ship is generally based on considering it to be a simple sum of the viscous resistance and the wave resistance. An experimental approach for predicting full-size ship resistance on this basis is practical but obviously has the deficiency that a model has to be built for each prototype of interest and the resulting tank tests are time consuming. On the other hand, purely theoretical calculations of the wave resistance, using, for example, the Michell theory, require relatively little computer time and give an excellent portrayal of the overall variation of the vessel resistance as a function of forward speed. Unfortunately, there are sufficient differences between this theory and the measured results to make this method impractical for design purposes. The proposal examined here is to use a data bank of experimental resistance results to modify the theoretical predictions. It is demonstrated that the technique will produce remarkably accurate resistance predictions and can take into account the effects of the water depth, any restriction of canal or river width, as well as the prismatic coefficient, and other geometric parameters.

• KSBB Journal
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• v.17 no.1
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• pp.106-109
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• 2002

Gel electrophoresis of DNA is a well known technique in molecular biology. This technique is simple, rapid to perform, and capable of adequately separating fragments of DNA. A number of mixtures of DNA fragments ("DNA size markers") are frequently employed in a purpose of extrapolating the sizes or the amount of DNA molecules during gel electrophoresis. DNA size markers are constructed by digesting plasmid DNA, bacteriophage DNA, or recombinant DNA molecules with one or more restriction enzymes. However, liquid suspension containing DNA size marker needs to be kept at a low temperature during storage and shipping. In an attempt to maintain the DNA samples at room temperature for extended period of time, lyophilization of DNA with addition of nuclease inhibitor was studied. Gel loading buffer was also added to the lyophilized DNA to provide additional convenience such that DNA size marker was the "ready-to-use" followed by simply reconstituting with distilled water.