• Journal of Pharmaceutical Investigation
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• v.35 no.1
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• pp.51-55
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• 2005

A high-performance liquid chromatographic method was employed for the determination of bumetanide in human plasma. After addition of internal standard (IS, naproxen) and acidification of the plasma with 1 M hydrochloric acid, the drug and IS were extracted into dichloromethane. The organic phase was back-extracted into 1 M sodium bicarbonate solution and 50 ${\mu}l$ of the aqueous phase was injected onto a reversed-phase C18 column with a mobile phase consisting of methanol: water: glacial acetic acid = 65 : 35 : 1. The samples were detected utilizing a fluorescence detector (excitation wavelength 235 nm, emission wavelength 405 nm). The method was specific and validated with a lower limit of 5 ng/mL. Intra- and inter-day precision and accuracy were acceptable for all quality control samples including the lower limit of quantification. The applicability of the method was demonstrated by analysis of plasma after oral administration of a single 2 mg dose to 24 healthy subjects. From the plasma bumetanide concentration vs. time curves, the mean AUC was $246.5{\pm}73.8\;ng{\cdot}hr/mL$ and $C_{max}$ of $132.1{\pm}40.9$ ng/mL reached 1.2 hr after administration. The mean biological half-life of burnet ani de was $1.1{\pm}0.2$hr. Based on the results, this simple and validated assay method could readily be used in any pharmacokinetic or bioequivalence studies using humans.

• Journal of the Korean Applied Science and Technology
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• v.35 no.3
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• pp.857-864
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• 2018

Demand for developing artificial green pearl that meets the needs of modern people has been increasing. In this paper, eco-friendly inorganic pearlescent pigment was used instead of organic pigment and urethane resin was substituted for nitrocellulose which has been used as main materials in previous preparation method, increasing gloss from 73.4% to 86.7%. Urethane was coated on substrate before finishing with CVD, resulting high gloss of 96%. Colorimeteric analysis shows that a* and b* of CIE value was changed from +37.7 and +24.5 to +31.9 and +14.2 respectively because of CVD finishing, obtaining colorful, high gloss and durable artificial pearl. Quality and toxicity of samples was established by chemical resistance, glossiness, colorimeter, surface roughness, wear resistance, content of heavy-metal, and salt water test.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.4
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• pp.437-444
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• 2013

Eight fecal sterols were analyzed in surface sediments collected from shellfish farming areas in Yeoja Bay and Gangjin Bay, Korea, to evaluate sewage-derived fecal pollution. The concentrations of coprostanol, a good marker of sewage-derived organic contamination, in sediments were in the range of 10-530 ng/g-dry in Yeoja Bay, and 10-190 ng/g-dry in Gangjin Bay. Coprostanol levels were markedly higher in the inner bay than in the outer bay. These levels were lower than those reported in urbanized bays in Korea, however, they were comparable to levels in other shellfish farming areas including Gamak Bay. A multivariate analysis of the ratios of other sterols suggested that the sterols originated from sewage and plankton/benthos. Sewage was the dominant source at stations located close to the river mouth and wastewater treatment plant (WWTP) outfalls, and plankton/benthos was the primary source in the outer bay. These results suggest that management of point sources, e.g., WWTP as well as non-point sources, e.g., riverine inputs is important for improving the water quality in Yeoja Bay and Gangjin Bay.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.6
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• pp.491-497
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• 2015

Traumatic brain injury (TBI) is a major cause of mortality and long-term disability, which can decrease quality of life. In spite of numerous studies suggesting that Epigallocatechin-3- gallate (EGCG) has been used as a therapeutic agent for a broad range of disorders, the effect of EGCG on TBI remains unknown. In this study, a weight drop model was established to evaluate the therapeutic potential of EGCG on TBI. Rats were administered with 100 mg/kg EGCG or PBS intraperitoneally. At different times following trauma, rats were sacrificed for analysis. It was found that EGCG (100 mg/kg, i.p.) treatment significantly reduced brain water content and vascular permeability at 12, 24, 48, 72 hour after TBI. Real-time PCR results revealed that EGCG inhibited TBI-induced IL-$1{\beta}$ and TNF-${\alpha}$ mRNA expression. Importantly, CD68 mRNA expression decreasing in the brain suggested that EGCG inhibited microglia activation. Western blotting and immunohistochemistry results showed that administering of EGCG significantly inhibited the levels of aquaporin-4 (AQP4) and glial fibrillary acidic protein (GFAP) expression. TBI-induced oxidative stress was remarkably impaired by EGCG treatment, which elevated the activities of SOD and GSH-PX. Conversely, EGCG significantly reduced the contents of MDA after TBI. In addition, EGCG decreased TBI-induced NADPH oxidase activation through inhibition of $p47^{phox}$ translocation from cytoplasm to plasma membrane. These data demonstrate that EGCG treatment may be an effective therapeutic strategy for TBI and the underlying mechanism involves inhibition of oxidative stress.

• Journal of Navigation and Port Research
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• v.31 no.6
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• pp.555-562
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• 2007

The environs of Korea in winter season are influenced by the distribution of atmospheric pressure, namely, the typical east-low and west-high pattern that is formed from both the Siberian continental high pressure and the Aleutian oceanic low pressure. In this reason, the violent West or North-West monsoon, the billows with the strong wind, and the tremendous heavy snowfall are encountered very frequently in the West Sea. In this study, the trajectories of the extratropical cyclone are analysed to choose the safe refuge areas of National Fishery Supervision Vessel using the surface analysis weather chart for 11 years from 1994 to 2004. The safe refuge areas according to the trajectories of the extratropical cyclone in the West Sea are decided using data that contain the topographical properties of island, the depth of water, the state of low quality, the influence of tidal current, and the distribution of fishing-net.

• YAKHAK HOEJI
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• v.52 no.1
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• pp.12-19
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• 2008

The organic acids (formic acid, acetic acid and propionic acid), phenol and benzopyrene in wood vinegar were determine by HPLC. An $Atlantis^{TM}dC_{18}$ column with a acetonitrile: 0.1% phosphoric acid (5: 95, organic acids), acetonitrile: water (10 : 90, phenol), 100% acetonitrile (benzopyrene) as a mobile phase was used. Retention time of acetic acid, formic acid, propionic acid, phenol and benzopyrene was 4.77, 3.73, 9.08, 30.97 and 6.10 min, respectively. The calibration curves of organic acids, phenol, benzopyrene were linear over the concentration range of $30{\sim}500,\;60{\sim}1000$, and $3{\sim}50\;{\mu}g/ml$ with correlation coefficient of above 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) of acetic acid, formic acid, propionic acid, phenol, and benzopyrene was 1.71 and 5.19, 1.11 and 3.35, 4.87 and 14.74, 6.45 and 19.55, 0.08 and $0.24\;{\mu}g/ml$, respectively. The coefficients of variation for intra- and inter-day assay were $0.21{\sim}4.14$ and $0.07{\sim}1.19%$, respectively and the precision was $95{\sim}115%$. From this study, we suggest that HPLC is suitable method for the determination of organics in wood vinegar and could be applied to quality control of wood vinegar.

• Journal of the Korean Geophysical Society
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• v.4 no.4
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• pp.267-285
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• 2001

This study has tried to develop the modified DRASTIC Model by supplying the parameters,such as structural lineament density and landuse, into conventional DRASTIC medal, and to predict the potential of groundwater contamination using GIS in Whanam 2 Area, Gyeonggi Province, Korea. Since the aquifers in Korea is generally through the joints of rock-mass in hydrogeological environment, lineament denisity affects to the behavior of goundwater and contaminated plumes directly, and land-use reflect the effect of point or non-point source of contamination indirectly. For the statistical analysis, lattice layers of each parameter were generated, and then level of confidence was assessed by analyzing each correlation coefficient. Composite contamination map was achieved as a final result by comparing modified DRASTIC potential and the amount of generation load of several contaminant sources logically. The result could suggest the predictability of the area of contamination potrntial in the respects of hydrogeological aspect and water quality.

• Journal of Korean Society of Rural Planning
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• v.1 no.2
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• pp.53-66
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• 1995

This study aims at analyzing of residents'attitudes and spatial structures in the rural villages reconstructed by the Rural Village Improvement Project(type A), and suggesting the guiding directions for planning rural villages. Six rural villages located in KyungGi province were selected for case study. The Major findings can be summarized as follows : 1) The ratio of type A project(newly planned village) in the total cases of the Rural Village Improvement Project has increased since 1990, In the majority(91.2%) of the type A project, the project area is below 2 ha and density of dwelling unit is below 40 households/ha. 2) The three spatial structures of newly planned rural village, i.e., Loop pattern, Cul-de-sac pattern, and Dendritic pattern, are identified. And the barns in the newly-build houses are categorized into the five types : the vertical addition type, the horizontal addition type, the vertical- horizontal addition type, the no- barn type, and the reused -barn type. 3) In the newly planned rural villages, the level of satisfactions for the quality of house and water & sewer system is remarkably higher than in the existing rural villages. These are the positive effects caused by the Rural Village Improvement Project. 4) The majority of the villagers think the improvement of the existing rural village, instead of redevelopment or newly planned village, is most desirable, which means the improvement of houses, roads, and sanitary facilities while preserving the spatial structures of the existing village.

• Journal of Wetlands Research
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• v.18 no.1
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• pp.94-99
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• 2016

Non-point source control has been issued and intensively studied for the control of water quality in streams and rivers. In this study, non-point source pollutants monitoring was conducted at the end of five small streams which were connected to a main stream in three stages including Phase I (one hr before the rainfall), Phase II (one hr after the rainfall), and Phase III (six hrs after the rainfall). For all five small streams, the concentrations and loading rates of non-point source pollutants significantly increased due to the rainfall. As a result of this, priority control streams and priority pollutants were suggested to prepare for future plans of the non-point source control.

• Journal of the Korean Association of Geographic Information Studies
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• v.9 no.2
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• pp.11-19
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• 2006

Sewerage is the system that improves the quality of human life and prevents many disasters such as floods. However the investigators in Korea only have been concerned about the sewer system, so the sewage treatment plant stays in the basic level like mapping. For example, only one attribute can be recognized in the linear object. Because of this limitation, it makes difficult to manage the linear attribute regarding to the sewage pipe plan. And it is impossible to control a partial (point type, line type) attribute changes of the linear object. We will therefore present the applicable method for the attribute changes of the linear object like the sewage pipe plans. For this reason, this paper is designed on the basis of Dynamic Segmentation(DS). DS has the advantage of giving the attribute value to the exact place in the linear object. As a result of using DS, the variety environment changes around the sewage pipes are applied to the building sewerage data. This also makes it possible to get a precise estimation for the maximum dirty water amount.