• 한국버섯학회지
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• 제19권3호
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• pp.126-133
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• 2021

In this study, we investigated the effect of solid culture medium on the production of cordycepin in Cordyceps militaris. The regression equation was expressed as follows: Y₁ = 755.3-58.6625X₁+4.79432E-14X₂-46.6625X₃-5.66269E-14X₁X₂-0.025X₁X₃+1.62475E-14X₂X₃-160.6625X₁²+0.0125X₂²-206.9625X₃², where, Y represents the value of cordycepin content (㎍/g), X₁ corresponds to the weight of M. alternatus in solid culture medium (g/bottle), X₂ to the water content of the solid culture medium (%), and X₃ to the culture period (day). The solid culture medium was optimized using the response surface methodology, and the optimal medium composition was as follows: the weight of M. alternatus in solid culture medium and water content were 16.2% and 100.7% (20.14 mL water/20 g solid culture medium), respectively, with a culture period of 39 days. Under these conditions, the cordycepin content of the fruiting bodies reached 150.0 ㎍/g (actual value). The supplementation of M. alternatus in solid culture for improved cordycepin content of C. militaris seems to be a promising alternative to wild and solid cultivation.

• 한국유기농업학회지
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• 제8권1호
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• pp.79-88
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• 1999

In order top investigate the growth of water dropwort grown by sandculture irrigated with recirculated tilapia aquaculture water, these experiments were carried out. Fish(tilapia) production and biofiltration provided by sand cultured water dropwort(Oenanthe stolonifera DC.) were linked in a closed system of recirculation water. Water dropwort was irrigated with water drawn from the tilapia tank and drainage from sand beds was returned to the fish tank. The temperature, pH and EC of tilapia culture water were stable. The growth of water dropwort grown by sandculture with aquaculture water was normal. Microbial activity of the biofilterbed irrigated by tilapia rearing water was higher than that of biofilterbed irrigated by tapwater. The feasibility of an integrated, recirculatory system for concurrent production of water dropwort and fish with no additional fertilization application was demonstrated.

• 한국식품영양과학회지
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• 제33권2호
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• pp.255-261
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• 2004

뽕나무가루 첨가 배지에서 배양한 느타리버섯균사체 배양물의 조추출물이 자유라다칼로 유도한 linloeic acid의 산화를 대조구에 비해 75.9% 감소시켰고, mouse liver microsome산화에서도 NADPH/Fe$^{++}$ system에서 64.3% 감소시켰다. 이와 같은 효과는 조추출물로부터 용매분획한 분획물의 단독 효과보다 우수하였다. 상황 및 동충하초버섯균사체 배양물의 조추출물도 느타리 버섯균사체 배양물의 조추출물과 효과가 유사하였지만 다소 낮았다.

• 원예과학기술지
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• 제31권4호
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• pp.423-428
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• 2013

Although closed soilless culture is useful for saving water and fertilizers with minimizing environmental pollution, adequate management of nutrient solutions is still not stabilized in greenhouse cultivation. In order to investigate the problems occurred in closed soilless culture of Paprika (Capsicum annuum L., cv. Fiesta), we compared ion balance, fruit yield, and the water and fertilizer use efficiencies in the closed system with those in the open system. The plants were grown in rockwool culture with a nutrient solution of EC $2.5dS{\cdot}m^{-1}$. After 4 weeks of treatment, individual ratio of $NO{_3}^-$, $K^+$, $Ca^{2+}$, and $Mg^{2+}$ to total ion concentrations ($meq{\cdot}L^{-1}$) decreased from the initial value, especially the biggest decrement was observed in $K^+$, and on the other hand, $SO{_4}^{2-}$, $Cl^-$, and $Na^+$ were accumulated in the closed system. Yields after four-time harvests were 19% higher in the open system than in the closed system. Total volume of water used per unit area ($m^2$) in the open system was 20% higher, but the total water use per fruit was not significantly different between the two systems, while t total fertilizer use per fruit was 78% higher in the closed system. Amount of marketable fruits was not significantly different between the two systems. We concluded that the increase in $K^+$ supply and the replenishment of recycled nutrient solution every four weeks were required for preventing the imbalance or depletion of nutrients in the close soilless culture of paprika plants to get more balanced nutrient composition during whole cultivation period.

• 미생물학회지
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• 제38권3호
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• pp.198-204
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• 2002

폴리오 바이러스는 전형적인 장관계 바이러스로 마비, 무균성 수막염, 뇌염 등을 유발한다. 폴리오 바이러스는 분변-구강 경로를 통해 전파되며, 오염된 물을 음용수로 사용할 시 공중 보건에 문제가 될 수 있으므로 먹는 물에서 폴리오 바이러스를 검출하는 것은 중요하다. 감염성이 있는 바이 러스와 불활성화(열처리와 자외선 처리)시킨 바이러스를 세포배양법, 역전사 중합효소 연쇄반응법(reverse transcription-polymerase chain reaction: RT-PCR)그리고 세포배양-중합효소 연쇄반응 통합법(integrated cell culture-PCR: ICC-PCR)으로 검출 실험을 했다. 감염성이 있는 폴리오 바이러스는 세 가지 방법으로 모두 검출이 되었으며, 이 중에서 바이러스를 검출하는데 ICC-PCR 방법이 가장 민감했다. 세포배양법은 적은 수의 바이러스를 검출하는데 약 2주의 긴 시간이 걸렸다. 열처리나 자외선 처리로 불활성화된 바이 러스는 세포배양과 ICC-PCR방법으로는 검출이 되지 않았다. 자외선 처리한 바이러스는 RT-PCR 방법으로 검출되지 않았으나 열처리한 바이러스는 검출되었다. RT-PCR 방법은 감염성 바이러스뿐 아니라 불활성화된 바이러스도 검출할 수 있으므로 감염성이 있는지 없는지를 구분할 수 없는 단점이 있다. 이와 같은 결과는 감염성 있는 바이러스를 가장 민감하고 효과적으로 검출하는 방법이 ICC-PCR 방법이라는 것을 제시하여 준다.

• ALGAE
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• 제21권1호
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• pp.133-139
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• 2006

Outdoor cultivation of cyanobacterium Spirulina platensis was carried out for 40 days in a batch mode. A half concentration of the SOT based on the underground water was used as culture medium. Working volume was 5.7 tons with 0.2 m depth. During cultivation, mean water temperature, DO and light intensity were all in proper conditions for the S. platensis growth. The adjustment of pH to over 10 with Na2CO3 and addition of the 1.5% natural salt were very effective to delete contaminant organisms, Chlamydomonas moewusii and Chlorella minutissima occurred one after the other in the culture. The mean productivity of the biomass based on the dry cell weight from 14 to 25 days, after the contaminants were deleted, was 7.8 g ·m–2· d–1, which was relatively high productivity in that a half concentration of the SOT was used for the culture. Underground water used in the culture minimized contaminants invasion and addition of the 1.5% natural salt was effective to delete contaminants as well as acted as mineral supplement in outdoor cultivation of S. platensis. Harvesting using the floating activity of S. platensis was effective from mass floating in day time after overnight without agitation and illumination.

• 상하수도학회지
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• 제12권4호
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• pp.78-85
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• 1998

Strains degrading and decolorizing reactive dyes, Procion blue HEGN and Procion red HE7B were isolated from water system, are named as RBK1 and RRK, the growth characteristics of which were investigated. Decolorization efficiencies after 42 hrs in batch culture were 95% and 77%, respectively. and the optimal culture condition of temperature and pH were $30^{\circ}C$, 7.0. Decolorization efficiencies in condition of aerobic shaking culture by strains RBK1 and RRK conspicuously increased, and culture by strain RBK1 was found as 95% after 42 hrs, while standing culture was 64%, Optimum nitrogen source was peptone, and It was found that decolorization efficiencies by strains RBK1 and RRK increased up to 4,000mg/l of peptone concentration as nitrogen source, but peptone concentration did' nt influence the decolorization efficiency in above 4,000mg/l. When the concentration of dyes were more than 800 mg/l and 400 mg/l respectively, the strains RBK1 and RRK, which degrade Procion bule HEGN and Procion red HE7B, showed a sharply decreased decolorization efficiencies; then the specific growth rate were $0.25hr^{-1}$ and $0.09hr^{-1}$.

• 한국농공학회지
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• 제45권4호
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• pp.55-65
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• 2003

Pilot study was conducted to examine the effects of ponded-water depth and reclaimed wastewater irrigation on paddy rice culture. For the ponded-water depth effect, three treatments of shallow, traditional, and deep water depths were applied, and each treatment was triplicated. The irrigation water for the treatment pots was an effluent from constructed wetland system for sewage treatment, while the control pot was irrigated with tap water kept traditional ponded-water depth. Irrigation water quantity varied with ponded-water depth as expected and drainage water quantity also varied similarly, which implies that shallow irrigation might save irrigation water and also reduce environmental impacts on downstream water quality. Rice growth and production were not significantly affected by ponded-water depth within the experimental condition, instead there was an indication of increased production in shallow and deep ponded-water depths compared to the traditional practice. Raising drainage outlet to the adequate height in paddy dike might be beneficial to save water resources within the paddy field. There was no adverse effect observed in reclaimed wastewater irrigation on the rice production, and mean yield was even greater than the control pots with tap water irrigation although statistically not significant. Water-saving irrigation by shallow ponded-water depth, raising the outlet height in diked rice paddy fields, minimizing forced surface drainage by well-planned irrigation, and reclaimed wastewater irrigation are suggested to save water and protect water quality. However, deviation from traditional farming practices might affect rice growth in long term, and therefore, further investigations are recommended before full scale application.

• Journal of Microbiology and Biotechnology
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• 제3권4호
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• pp.285-291
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• 1993

Growth responses of some intestinal bacteria such as bifidobacteria and Clostridium perfringens to the extracts of certain foodstuffs were investigated in vitro. Among edible mountain herbs, the extracts of several chui-na-muls (Aster tataricus, Ligularia fischeri and Aster scaber) had an inhibitory activity against C. perfringens on the agar plate and the water extract of Aster scaber worked selectively on it among intestinal bacteria. The water extract showed growth-promoting effect toward bifidobacteria such as B. adolescentis, B. animalis, B. bifidum, B. infantis and B. thermophilum in the broth culture. When the faecal inoculum was incubated in the culture with the extract, the population of C. perfringens decreased, whereas that of bifidobacteria increased by $10^3$ scale.$\beta$-glucuronidase activity in the culture with the water extract of Aster scaber digested with pepsin and pancreatin was lower than that in the control culture.

• Toxicological Research
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• 제14권2호
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• pp.171-176
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• 1998

The biological effects of the water extracts of Rhus Verniciflua Stokes (RVS) were evaluated by protection against hydroxyl radicals. Antioxidative activities were measured using both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and thiocyanate method. Also we used the Glucose oxidase (GO) 20 mU/$\textrm{m}{\ell}$ hydroxyl radical generating system in mouse forebrain cell culture. Water was used for ex-traction from RVS as a solvent which has high polarity especially. In DPPH method, the antioxidative activities of the crude water extract were stronger than any other extracts of low polar-solvents. In the antioxidative effects of mouse forebrain culture using 20 mU/$\textrm{m}{\ell}$ GO, cell viabilities were evaluated 65.6%, 68.8% at 1 $\mu\textrm{g}$. 5 $\mu\textrm{g}$ addition of crude water extracts (30 mg/$\textrm{m}{\ell}$) respectively. 10 $\mu\textrm{g}$ addition of crude water extracts had more than 86.1% cell viabilities, P<0.0l significantly, compared with the group treated with GO alone. In comparison with the antioxidative activities of several commercial antioxidants (ascorbic acid, $\alpha$-tocopherol, catalase, serum), 273 $\mu\textrm{g}$/$\textrm{m}{\ell}$ addition of crude water extracts (300 $\mu\textrm{g}$/$\textrm{m}{\ell}$) showed equivalent antioxidative effect to 25 uM ascorbic acid.