• 한국약용작물학회지
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• 제10권1호
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• pp.24-28
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• 2002

작약의 양질약재 생산을 위한 기초 자료를 얻고자 건조과정중 박피시간별 절단과 무절단 그리고 열풍건조 온도를 달리하여 시험한 결과를 요약하면 다음과 같다. 박피시간이 길어질수록 건조시간은 통뿌리보다 절단한 것이 건조온도가 단축되었으며, $30^{\circ}C$에서 $60^{\circ}C$까지의 건조온도에서 건조온도가 상승할수록 온도상승의 효과는 감소하였다. 작약의 주 약용성분인 paeoniflorin 함량은 무박피 절단형태로 열풍온도 $30^{\circ}C$에서 건조한 작약이 3.48%로 가장 높은 함량을 유지하였고, 120분 박피후 통뿌리로 $60^{\circ}C$에 열풍건조한 작약은 2.29%로 건조과정중 많은 양이 손실되어 가장 낮게 나타났다. 건조작약의 외부색택은 통뿌리로 $60^{\circ}C$에서 열풍건조했을 때 흑갈색으로 변하는 정도가 가장 심했다. 따라서 작약 수확후 건조과정 중 건조시간 단축과 약효성분 그리고 색택면에서 건조전의 처리 방법은 무박피 절단형태가 장시간 박피 원형형태보다 우수한 것으로 판단된다.

• 한국식품저장유통학회지
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• 제20권6호
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• pp.871-876
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• 2013

오미자의 위생과 저장성에 관여하는 미생물(총 호기성세균, 효모 및 곰팡이균)의 저감을 위해서 세척수 종류(물, $ClO_2$, $H_2O_2$) 및 농도별 이산화염소수($ClO_2$ 10 ppm, $ClO_2$ 15 ppm, $ClO_2$ 20 ppm, $ClO_2$ 25 ppm, $ClO_2$ 30 ppm)처리에 따른 세척시간(30~90초) 및 세척배수(${\times}1{\sim}{\times}4$)을 달리 처리하여 오미자 표면에 존재하는 총 호기성 세균수, 효모 및 곰팡이균수를 측정하였다. 오미자의 세척수 종류에 따른 미생물 균수 측정 결과, 세척하지 않은 구(control) 및 물세척 처리구보다 이산화염소수 처리구 및 과산화수고 처리구가 총세균, 효모 및 곰팡이 균수가 감소하였음을 확인하였다. 이산화염소($ClO_2$)수를 이용한 오미자의 세척시간에 따른 미생물 균수 측정 결과 총 세균과 효모 및 곰팡이는 이산화염소 농도 30 ppm 에서는 30초, 15 ppm에서는 60초, 10 ppm에서는 90초 처리시 검출되지 않았다. 또한 세척배수에 따른 미생물 사멸 효과는 이산화염소 농도 30 ppm 에서는 ${\times}1$, 20 ppm에서는 ${\times}2$, 15 ppm에서는 ${\times}4$로 처리시 총 세균과 효모 및 곰팡이가 측정되지 않았다. 이런 결과로 미루어 보아 이산화염소수 처리구는 이산화염소수의 농도가 증가하고 세척 배수가 증가함에 따라 미생물 저감화 효과를 확인할 수 있었다.

• 한국학교ㆍ지역보건교육학회지
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• 제3권
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• pp.79-110
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• 2002

The purpose of this study was to provide basic data necessary for creating a more successful health enhancement program, health education and health policy which can enhance health management abilities and enable a healthy school life by examining relations between high school students' health perception, who are in early adolescence, and their health promotion behavior. The subjects in this study were 813 students from 8 high schools on Jeju Island. The collected data were analyzed with t-test and one-way ANOVA through questionnaires by researcher on the basis of advance research. The findings of this study were as belows : 1. When general characteristics were considered, health perception was more significantly affected by those who were boys, whose family income was on a higher level, who scored better in school, whose parents were more concerned about their children's health, and who had experiences of being hospitalized. Especially, the more parents were concerned about their children's health, the higher the child's self-confidence on health(p<.05), anxiety on health(p<.05), practicality on disease prevention(p<.05), self-confidence on future health(p<.01), health care ability(p<.01), and family roles on health(p<.05). The less parents were concerned about their children's health, the higher the child's anxiety on disease(p<.01) and uncertainty on future health(p<.01). 2. When students' health promotion behavior was concerned, in the area of Eating Habits, having regular meals(72.9%), moderate consumption of meat(89.7%) and a balanced diet(64%) were common, whereas having a regular breakfast(37.4%) and eating vegetables and fruits(43.6%) were rare. In the area of Exercise, all subareas such as exercising 3 or 4 times a week(76.7%), more than one hour of exercise at a time(74.9%), a short distance walk(51.8%), exercise within one hour after meals(87%), and whether wanning up or not(88.6%) were above average. In the area of Personal Hygiene, all subareas such as drinking boiled water(57.3%), washing hands after using the bathroom(71%), brushing teeth after eating(42.4%), washing up after going out(50.3%), and washing hair and taking a frequent bath(77.2%) were rare. In the area of Control of Personal Relations, consulting personal problems with family(78.7%) had a positive response, whereas meeting someone new(59.8%), maintaining sound relations with the opposite sex(47.3%), having respect for one's opinion(51.5%), and maintaining peaceful relations with people(58.4%) had rare negative results. In the area of Stress Management, not being competitive(69.4%), releasing problems(62.4%) and sleeping soundly(66.2%) were common, whereas having your own ways to relieve stress(46.8%) and having your own dreams and hopes(58.5%) were rare. 3. When general characteristics were considered, as far as relations of health promotion behavior were concerned, the boys were significantly different in the area of Eating Habits(p<0.01) and Exercise(p<0.01), and the girls were in the area of Personal Hygiene. As family economic status was high and parents were concerned about their children's health, the entire areas of health promotion behavior were significantly different. Whether or not they were hospitalized made no significant difference in statistics. 4. When their regions were considered, it comparing city to town, there was no statistically significant difference in health perception and relations of health promotion behavior according to general characteristics. As seen in the results above, high school students' level of health perception and level of practicing health promotion behavior were generally low. In conclusion, by enhancing health perception, a plan for activating health education, which can implement positive health promotion behavior, should be made.

• 복식
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• 제14권
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• pp.145-165
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• 1990

This study examine closely conditions of costume between the Modernized period and Liberation with newspaper materials. Because newspaper generally appear society conditions in those days on rapid and across-the boad basis. The Modernized period is extremely change among history of costum (ordinance prohibiting top knots, allowance of foreign clothes putting on). Because this change have been spontaneously not by internal desired but Western input by the strong nation of imperialism to enclose Chosun, they was accepted by the general public later under the rule of Japaneses Imperialism. Consequently, study of costume play an important part periods between the Japanese annexation of Korea and Liberation. This study apply to newspaper characteric for costume, and closely examine an important costum condition of those days next time, and present costume material in those days that composed the account catalog appeared periods between the first publication(1920) of the Dong A II Bo, and in the year 1945, it is as follows. 1. Foreign clothes of men generally accept the general public on look at from form change, in the 1920's had come short Jackets and narrow throusers into fashion, in the 1930's had come trousers of generous waist band with broads shoulder and long Jackets. Catalog of Major clothes is as follows; Spring coat, Jacket, Vest, Shirt, etc. While pants had come trousers into fashion 2. Functional characteric of Foreign clothes was the possible acceptance of women's foreign clothes. It relate with much discussion to improve Korean development in those days and substitute foreign clothes for Korean clothes because of institence in those days to improve functional clothes life. 3. An improvement women's Korean clothes generally take aim at women's nipple liberation, substitute vest waist for skirt waist, appear seamless one-piece skirt of shade length, and long dress length of Jacket. 4. Children's clothes give an account of functional and sanitary conditions, handling method, washing method. 5. Clothes materials give account of foreign clothes material, artificial silk, furs, cotton fabrics, and etc. 6. Clothes management give an account of washing, keeping method, washing method of foreign clothes, and keeping of furs. 7. The hair generaly had come short hair into fashion in men's case, while accounts on long hair fashion of foreign nation effect in case of women. 8. Describing on beauty care manage primary beauty care, reform, plastic operation, and shade beauty care. Ideal beauty care deal with natural and dignified buauty care. 9. Accesaries (hat, handbag, handkerchief, gloves) change with fashion of clothes, it rapid more than clothes fashion. 10. On encouragement of abolition of white clothes and putting on dyeing clothes, because of economic defect of white clothes, psychology and beauty consequently, white clothes is on the rise abolition. In national level almost substitute dyeing clothes for control and improvement of people of all social standings consequently, dress and its ornaments conditions in those days analyzed account of Dong-A II Bo accept the foreign clothes that introduced internal country of the whole century, and substitute dyeing for white clothes. Costume condition in those days appear the mixed conditions of Korean clothes and Foreign clothes. In the 1920's is the first consideration dress and its ornaments form of Korean clothes. As later goes on foreign is given much weight in the whole clothes life. Account of foreign clothes managemental ways appear in the 1920's, while those facts prove the point that appeared the account that always dealed with concrete content of foreign fashion in the 1930's.

• 한국수정란이식학회지
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• 제9권1호
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• pp.7-14
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• 1994

The present experiments on cryopreservation were designed to examine the effects of solution toxicity, equilibration time and cell stages on the post-thaw survival of bovine IVF embryos. The oocytes were matured in vitro(IVM) for 24 hrs. in TCM-199 supplemented with 35 $\mu$g /ml FSH, 10 $\mu$g /ml LH, 1 $\mu$g /ml estradiol-17$\beta$ and granulosa cells at 39$^{\circ}C$ under 5% $CO_2$ in air. They were fertilized in vitro(IVF) by epididymal spermatozoa treated with heparin for 24 hrs., and then the zygotes were co-cultured in vitro(IVC) with bovine oviductal epithelial cells for 7 to 9 days. The bovine IVF embryos were exposed to the EFS solution in one step at room temperature, kept in the EFS solution during different period for toxicity test, vitrified in liquid nitrogen, and thawed rapidly. 1. after the bovine blastocysts were exposed to EFS solution for 2 min. at room temperature and then they were washed in 0.5 M sucrose solution and TCM-199, they were cultured to examined cryoprotectant induced injury during exposure, Most of the embryos(95.0%) developed to reexpanded blastocoels. However, when the exposure time was extended to 5 and 10 min, these development rates dropped dramatically in 5 min. (69.5%) and 10 min. (47.4%), respectively, 2. When the bovine IVF embryos were vitrified in EFS solution after the equilibration for 1 and 2 min. exposure, The embryos to have reexpanded blastocoels following thawing, washing and culture processes were found to he 82.6 and 73.9%, respectively. However, when the exposure time was extended to 3 min, this survival rate dropped to 18.2%. The optimal time for equilibration of bovine IVF blastocysts in EFS solution seemed to he 1~2 min. 3. When the bovine IVF embryos were equilibrated for 1 min. the significantly (P<0. 05) higher post-thaw survival rates were obtained from the embryos of blastocyst stage(81.3%) than morulae stage(5. 1%). The optimal cell stage for viterification with EFS solution proven to he blastocyst stage in bovine IVF embryos. 4. The number of blastomeres of blastocyst stage was examined with nuclear staining with Hoechst 33342 during 7 to 9 days post-insemination. The cell counts of frozen bovine IVF embryos were found significantly(P$\geq$7.5 and those of the fresh embryos 76.6$\geq$7. 1, which were cultured in the sarne period and conditions as frozen embryos.

• 한국식품위생안전성학회지
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• 제16권3호
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• pp.168-177
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• 2001

본 연구에서는 서울 소재 초등학교와 고등학교 급식의 위생관리 실태를 분석하므로 학교급식의 안전성을 확보하여 식중독 사고예방 및 급식품질 개선을 이루고자 하였다. 이를 위해 HACCP에 기준한 위생관리 평가도구를 설문지로 개발하여 관리자들이 자가 평가하게 하고,그 결과를 분석하여 위생관리 실태를 파악하고 취약한 부분을 규명하였다. 위생관리 평가도구는 온도 소요시간, 개인위생 및 기기 설비위생의 3영역으로 구분 하여 33문항, 5문항, 15문항씩 총 53문항으로 구성하였다. 평가문항에 대해서는 5점척도를 이용하여 표시하도록 하였다. 조사된 학교는 초등학교 98.4%(253개교), 고등학교 1.6%(19개교)였다. 세 영역중 개인위생에 관한 수행수준은 평균 4.06$\pm$0.57로 나타나 가장 잘 수행되는 것으로 조사되었다. 기기.설비위생의 수행수준은 평균 3.84$\pm$0.53로 나타났고, 온도.소요시간은 평균 3.45$\pm$0.46으로 나타나 보통정도의 수행수준으로 조사되었다. 특히 전체 문항중 수행수준이 가장 낮은 것으로 조사된 ‘조리 후 보관(2.03$\pm$0.94)’의 경우는 현행 학교급식업체들이 조리가 끝난 식품을 취급할 열장 또는 보온 기기, 냉장고를 거의 구비하지 못하고 있으며, 조리된 음식이나 차게 배식하는 음식의 적정 온도유지를 위한 온도계 사용이 전혀 이루어지지 않고 있기 때문인 것으로 사료 된다. 기기 설비위생영역에서 가장 낮은 수행정도를 보인 ‘싱크대의 용도별로 분리사용 여부(3.03$\pm$1.10)’와 ‘손 씻는 시설의 적절한 장소 위치 여부(3.07$\pm$1.13)’의 수행수준을 향상시키려면 적절한 개수의 싱크대를 구비해야 하며, 조리실내에 손 씻는 시설을 갖추어야 할 것이다. 결론적으로 학교급식 안전성 확보를 위한 위생관리 업무 향상을 위해서는 본 연구에서 가장 취약한 부분으로 드러난 시설 및 기기들을 우선 보완해야 할 것으로 사료된다.

• 핵의학기술
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• 제26권2호
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• pp.15-19
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• 2022

전립선암 환자의 전이성 질환 진단을 위해 사용되는 양전자 방출 단층촬영용 [⁶⁸Ga]PSMA-11 주사액은 자동화 생산 방법을 통해 높은 재현성과 우수한 방사화학적 수율 및 순도를 얻을 수 있으며 제조 시 작업자의 방사선 피폭을 최소화할 수 있다. 이를 위해 적용한 비 카세트 방식과 카세트 방식의 자동 합성 방법을 소개하고 비교하고자 한다. [⁶⁸Ga]PSMA-11 주사액의 자동화 생산을 위해 ⁶⁸Ge/⁶⁸Ga generator 50 mCi(iThemba LABS, Johannesburg, South Africa), 주사기 펌프 NE-1000(New Era Pump System, New York, USA)을 사용하였으며, 자동 합성 장치는 비 카세트 방식의 TRACERlab FXN pro(GE Healthcare, Liege, Belgium)와 카세트 방식의 BIKBox(BIK THERAPEUTICS Inc., Seongnam-si, Republic of Korea)를 사용하였다. 0.6 N 염산 용액 6 mL의 주사기가 장착된 실린지 펌프를 ⁶⁸Ge/⁶⁸Ga generator의 inlet-line과 연결하고 outlet-line은 자동 합성 장치와 연결한 후 자동 합성장치와 동시에 작동하였으며, 2 mL/min의 속도로 ⁶⁸Ga을 용출하였다. 초기 약 1.7 mL은 waste vial로 용출 시켰고, 그 후 2.5 mL은 반응용기로 용출하여, 방사능 농도가 높은 2.5 mL의 용액만 표지 과정에 이용하였다. 반응 용액의 pH를 HEPES buffer 용액으로 조절한 후 전구체와 95 ℃에서 15분간 반응하였으며, C18 light 카트리지를 이용, 분리·정제 하였다. 50% 에탄올/생리식염수 희석액으로 최종 화합물을 용출하고 생리식염수를 추가한 후 멸균 필터 함으로써 제조를 완료하였다. 각각의 자동 합성 장치에서 제조된 [⁶⁸Ga] PSMA-11 주사액의 품질관리를 시행하고 장단점을 비교하였다. 총 합성 시간은 각각 25±3분(비 카세트 방식), 23±3분(카세트 방식)이 소요되었으며, 방사화학적 수율은 멸균 필터 후 비 카세트 방식이 65.5±5.7%(n=45, non-decay corrected), 카세트 방식이 61.6±4.8%(n=98, non-decay corrected)였다. 비 카세트 방식은 장비 세척과 시약 준비 시간으로 인해 합성 전 준비 시간이 약 120분 소요되었고, 카세트 방식은 세척과 시약 준비 과정이 없어 합성 전 준비 시간은 약 20분 소요되었다. 비 카세트 방식 자동 합성 방법은 방사화학적 수율과 비용적 측면에서 카세트 방식 대비 높은 장점을 가지나, 제조 준비 단계에서의 편의성과 장비 유지 보수 측면에서는 카세트 방식이 장점을 가진다.

• Journal of Animal Science and Technology
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• 제62권5호
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• pp.668-681
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• 2020

The purpose of this study was aimed to isolate a Salmonella Typhimurium-specific phage (KFS-ST) from washing water in a poultry processing facility and to investigate the feasibility of the KFS-ST as a novel bio-receptor for the magnetoelastic (ME) biosensor method. KFS-ST against S. Typhimurium was isolated, propagated, and purified using a CsCl-gradient ultracentrifugation. Morphological characteristics of KFS-ST were analyzed using transmission electron microscopy (TEM). Its specificity and efficiency of plating analysis were conducted against 39 foodborne pathogens. The temperature and pH stabilities of KFS-ST were investigated by the exposure of the phage to various temperatures (-70℃-70℃) and pHs (1-12) for 1 h. A one-step growth curve analysis was performed to determine the eclipse time, latent time and burst size of phage. The storage stability of KFS-ST was studied by exposing KFS-ST to various storage temperatures (-70℃, -20℃, 4℃, and 22℃) for 12 weeks. KFS-ST was isolated and purified with a high concentration of (11.47 ± 0.25) Log PFU/mL. It had an icosahedral head (56.91 ± 2.90 nm) and a non-contractile tail (225.49 ± 2.67 nm), which was classified into the family of Siphoviridae in the order of Caudovirales. KFS-ST exhibited an excellent specificity against only S. Typhimurium and S. Enteritidis, which are considered two of the most problematic Salmonella strains in the meat and poultry. However, KFS-ST did not exhibit any specificity against six other Salmonella and 27 non-Salmonella strains. KFS-ST was stable at temperature of 4℃ to 50℃ and at pH of 4 to 12. The eclipse time, latent time, and burst size of KFS-ST were determined to be 10 min, 25 min and 26 PFU/ infected cell, respectively. KFS-ST was relatively stable during the 12-week storage period at all tested temperatures. Therefore, this study demonstrated the feasibility of KFS-ST as a novel bio-receptor for the detection of S. Typhimurium and S. Enteritidis in meat and poultry products using the ME biosensor method.

• 대한한의학원전학회지
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• 제24권2호
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• pp.23-50
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• 2011

"LeiGongPaoZhiLun" is the first pharmaceutical book, and there are various opinion on when LeiXiao(雷斅), the author lived. From the aspect of several data, it is appropriate most that LeiXiao live in the period of Sui(隋) Dynasty, like SuSong(蘇頌) in the Sung Dynasty said, This book was not made at one time by one person. At the beginning, it was written by LeiXiao and the later generations enlarged to finish. The original of "LeiGongPaoZhiLun" disappeared already but a large amount of this book was quoted to "ZhengLeiBenCao(證類本草)" to be preserved. The contents of "LeiGongPaoZhiLun" are the first professional book about processing of medicinal, in which most of processing of medicinal that is generally used today, is included and some methods are unused. Besides, the regulations of this book are specific, the range is broad, and theoretical frame is established for the first time. This book has abundant contents, in which several processing of medicinal are written. There are 10 methods such as steaming(蒸法), boiling(煮法), baking(炮法), stir-frying(炒法), calcining(煅法), long time boiling(煉法), scorching(燒法), baking after wrapping(煨法), immersing(浸法), washing(洗法), flying(飛法), etc. How to remove some section that is not used for drug, how to separate according to medical use and region to be applied, how to smash, how to cut, how to dry, container for medicine, warning for making a prescription, time to make medicine and amount of medicine, how to distinguish superior and inferior and origin, how to make medicine partially, etc, are contained in this book. The contents of "LeiGongPaoZhiLun" can be mixture of processing of medicinal of medical scientists and taoists. In conclusion, however, the effect by the later medical generations of "LeiGongPaoZhiLun" was not great on processing of medicinal. It stemmed from the difference of processing of medicinal in this very taoistic book that realistic difficulties were disregarded by taoists for training and practical purpose, treatment that is the reason that later medical generations used processing of medicinal. Consequently, there is no great relation between the development of processing of medicinal in the Sung Dynasty and this book. "LeiGongYaoXingFu(雷公藥性賦)" that was abundant in medical market in Ming(明) and Qing(清) period has no relation with "LeiGongPaoZhiLun" is kind of a distribution-purposed book that was mnge in or after Ming Dynasty. However, since a book that is said to be written by 'LiGao(李杲)'PaoZhihis nof a dijust borrowed and focus on a brief summery, it is not desirable for beginners to learn medical knowledge. "LeiGongPaoZhiLun" is the first pharmaceutical book and is a model to show how a science in the history of the herb medicine generated and developed and how such a book is changed and modified to make a change of value.

• 한국버섯학회지
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• 제4권4호
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• pp.135-143
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• 2006

1995년이후부터 재배되어온 큰느타리버섯은 배양중 오염율 증가, 발이상태불량, 기형버섯의 발생, 수량 격감 등 이른바 연작장해로 불리어지는 재배상 문제점들에 대한 원인파악과 해결책이 요구되어, 전국의 큰느타리버섯 주요 재배농가에서의 종균관리, 배지제조, 배양 및 생육관리 등 전반적인 재배실태와 연작장해 발생정도를 조사한 결과와 균배양, 버섯발생, 자실체 생육과정에서 발생된 병원균을 분리하여 동정한 결과는 다음과 같다. 가. 재배실태조사 결과 재배사 청결관리가 미흡하고 수확 후 재배사 세척 및 소독이 소홀하였다. 나. 발이유기시 습도가 90%이상, 환기는 소량 또는 억제하여 관리한 농가들에서 발이 유기부위에서 세균 및 곰팡이에 의한 오염과 기형버섯 발생 등으로 인해 수량이 낮았다. 다. 자실체 생육시 발이개체수가 과다하였고 환기 및 습도관리 미숙으로 자실체 갈변, 환기장해 등으로 자실체 생육이 불량하였다. 라. 강제흡기-강제배기 방식보다 강제흡기-자연배기 방식이나 자연흡기-강제배기 방식의 농가가 많아 발이 유기시 많은 양의 환기를 필요로 하는 큰느타리버섯 재배에 부적합한 환기시스템을 갖추고 있었다. 마. 큰느타리버섯 재배농가에서 수집한 병원균은 총 28점으로 세균13점, 곰팡이15점이었고 채집부위별로는 배양병에서 8점, 발이유기부위에서 12점, 자실체에서 8점을 분리하였다. 바. 큰느타리버섯 재배시 문제가 되는 세균은 Pseudomonas sp. Erwinia sp 가 많았는데, 발이유기 배지표면 오염과 자실체 갈변 및 괴사와 관련된 병징을 유발하였고, 곰팡이는 대부분 Trichoderma sp. 이었으며 주로 배양병 및 발이유기부위에서 발병하였다.