• 제목/요약/키워드: Washing and disinfection

검색결과 77건 처리시간 0.023초

치과용 Ni-Cr합금 용출배지에 의한 인간 피부 섬유아세포 성장도 관찰 연구 (A Study on the Viability of Human Dermal Fibroblast Cell by Media for Ni-Cr alloy elution)

  • 김갑진;최성민;김치영
    • 대한치과기공학회지
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    • 제31권3호
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    • pp.21-26
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    • 2009
  • Purpose: Standards of alloy for porcelain fused to metal crown be classified by metallic factor and biological factor. Metallic factors consist of stability of alloy composition and mechanical strength and surface characteristics for chemical bond. Biological factors be considered properties of metallic elements and problems originated by toxicity and hypersensitive reaction. Alloys considered such controversial points are the most suitable alloy for dental instrument. Method: Alloys added Be and Nb using Ni-Cr alloy which has been widely used for dental instrument be selected and classified experimental group. Non-addition Be and Nb to Ni-Cr alloy classify control group and addition Be alloy is Be-experimental group, addition Nb alloy is Nb-experimental group. Specimens for cytotoxicity analysis gave effect to washing and sterilization. and then made an experiment on elution with cell medium after disinfection. It conducted specimens within cell medium with 24hours, 48hours, 72hours, respectively. It cultured human dermal fibroblast(HDF) using cell medium for cytotoxicity test and then investigated elution rate through spectroscopic analysis by MTT-assay. Result: As results of cytotoxicity test by MTT-assay, cultured cell rate of VII measured more low numerical value within elution medium for 24hours focused on control group. Also, cultured cell rate of K3 alloys observed low value for 48hours, 72hours than value of control group. Conclusion: According to final result that synthesize above results, Ni-Cr alloy added Be and Ni has little difference in Cytotoxicity by MTT-assay.

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Effect of Chlorine Dioxide Gas Application to Egg Surface: Microbial Reduction Effect, Quality of Eggs, and Hatchability

  • Chung, Hansung;Kim, Hyobi;Myeong, Donghoon;Kim, Seongjoon;Choe, Nong-Hoon
    • 한국축산식품학회지
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    • 제38권3호
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    • pp.487-497
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    • 2018
  • Controlling of microorganisms in the industrial process is important for production and distribution of hatching and table eggs. In the previous study, we reported that chlorine dioxide ($ClO_2$) gas of a proper concentration and humidity can significantly reduce the load of Salmonella spp. on eggshells. In this study, we compared microbial reduction efficacy on egg's surface using hatching eggs and table eggs, internal quality of table eggs, and hatchability after both the conventional method (washing and UV expose, fumigation with formalin) and $ClO_2$ gas disinfection. Application of 40 ppm $ClO_2$ gas to the table and hatching eggs, respectively, reduced the aerobic plate count (APC) with no statistical difference compared with the conventional methods. Additionally, we didn't observed that any significant difference in albumin height, Haugh unit (HU), and yolk color, this result confirms that 40 ppm $ClO_2$ had no effect on the internal quality of the table eggs, when comparing with the UV treatment method. The hatchability of hatching eggs was not statistical different between formaldehyde fumigation and 80 ppm $ClO_2$ gas treatment, though the value was decreased at high concentration of 160 ppm $ClO_2$ gas. From these results, we recommend that $ClO_2$ gas can be used as a safe disinfectant to effectively control egg surface microorganisms without affecting egg quality.

서울지역 대학병원의 방사선과 카세트 소독에 관한 연구 (A Survey of the Radiographic Cassettes Disinfection of University Hospitals in Seoul)

  • 권대철;김문선;김동성;박범
    • 대한방사선기술학회지:방사선기술과학
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    • 제24권2호
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    • pp.65-70
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    • 2001
  • The purpose of this study is to prevent nosocomial infection in patients through contact of radiographic cassettes. Data were collected from radiographers working in 29 university hospitals in Seoul in February and March 2001. Radiographic cassettes were disinfected daily in 5 hospitals, weekly in 4 hospitals, monthly in 5 hospitals, bimonthly in 1 hospital and once every three months in another hospital. 12 other hospitals do not practice regular disinfections of radiographic cassettes. Gauze soaked in disinfectant solution is used in 7 hospitals while 11 hospitals used cotton and cloth soaked in disinfectant solution to clean the radiographic cassettes. 26 hospitals used 99% alcohol based disinfectant solutions while 3 hospitals used 75% alcohol based disinfectant. 26 hospitals use of intercourse cassettes outpatients and in patients. In 26 hospitals, all patients shared the same set of radiographic cassettes used in the hospitals, or in 26 hospitals, separate sets of radiographic cassettes are used for outpatients and inpatients. Separate sets of cassettes are used for ICU and inpatients in 6 others hospitals. 23 hospitals used the same sets of radiographic cassettes for all their patients. radiographic cassettes are cleaned in wash area in the study room of the radiographic department in 17 hospitals. 12 other hospitals do not have designated cleaning areas for the cassettes. All radiographers practiced hands washing with soap. All 29 hospitals surveyed have infection control committee. However, only 9 out of the 29 hospitals surveyed provided Infection disinfections control education to radiographers. Only 3 hospitals have radiographers sitting in the infection control committee. Infection management education is conducted in 63 hospitals annually, twice a year in 1 hospital and once every 3 months in 2 hospitals.

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치과위생사의 환자안전문화인식과 감염관리활동 (Patient Safety Culture Among Dental Hygienists and Perception of Infection Control Activities)

  • 정용주;이선미
    • 대한통합의학회지
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    • 제10권3호
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    • pp.161-172
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    • 2022
  • Purpose : The study was to promote patient safety by analyzing the effect of dental hygienist's perception of patient safety culture on infection control activities. Methods : The study is based on a survey of 210 dental hygienists in total working in dental settings. To find out infection control activities according to patient safety culture awareness, there were 6 general characteristics, 3 teamwork within the department, 2 infection control systems, 4 surface management, 9 equipment washing, disinfection, and laundry management, 4 infectious wastes, and 3 personal protection phrases.The data was analyzed using the SPSS version 20.0, and p<.05 was adopted to decide on significance. Results : The longer dental hygienists have worked n the dental settings, the more active they become in infection control activities. Among the different types of dental care settings, general (university) hospitals had the largest number of infection control activities, followed by dental clinics, and network dental clinics, in descending order. The dental settings possessing a higher number of dental hygienists were found to conduct more infection control activities than other dental settings. In addition, it was found that when a dental setting adopts a patient safety policy across all the units in the hospital, more systems and procedures for patient safety tend to be established, and that stricter management response to error leads to improvement of infection control activities. Conclusion :In order to enhance infection control activities, infection control activity programs should develop and implement periodic reinforcement of infection control education. regular monitoring of infection control activities.

로봇 착유시스템의 착유성능 및 착유량에 미치는 영향 (The Effect of the Milk Yield and Performance Analysis of Robot Milking System)

  • 김웅;이대원
    • 한국축산시설환경학회지
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    • 제15권1호
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    • pp.29-36
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    • 2009
  • 부족한 노동력 해소와 유질 및 유량의 향상을 위해서 5축 다관절 매니퓰레이터와 착유컵 착탈 시스템 및 영상처리 시스템을 이용한 로봇 착유시스템을 개발하였으며, 착유우 실험을 통해 시스템 성능분석 및 착유량에 미치는 영향을 알아보았다. 1. 착유컵 장착시간은 최소 17초, 최대 38초, 평균 27초가 소요되었으며, 이는 착유성능에 영향이 없을 것으로 판단되었다. 착유컵 탈락은 약 1초 내에 유두에서 탈락하는 것으로 나타나 착유작업에 지장을 주지 않을 것으로 판단되었다. 2. 로봇 착유시스템을 이용한 착유량은 평균 착유량은 7.5 kg으로 나타났으며, 주간 착유량의 표준편차는 0.7 kg으로 나타났다. 비유곡선을 이용한 착유량 분석도 로봇 착유시스템에 의한 착유 시 착유량에 큰 영향을 미치지 않은 것으로 판단되었다. 본 연구에서 개발된 로봇 착유시스템은 착유컵 착탈 성능과 착유량에 미치는 영향에 관한 것으로 착유실내의 모든 착유작업을 수행하기 위해서는 착유전 유두세척, 유두 마사지 및 전처리 공정과 착유우 후처리 및 소독 공정이 모두 필요한 사항이며, 앞으로 연구가 필요할 것으로 사료된다.

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ATP Bioluminescence와 간이미생물배지를 이용한 고등학교 급식시설의 위생상태 조사연구 (Evauation of Hygienic Status of High School Foodservice using ATP Bioluminescence Assay & Microorganism Test Strips)

  • 장재선;황성희
    • 한국식품영양학회지
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    • 제28권5호
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    • pp.918-925
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    • 2015
  • An investigation was conducted to evaluate the hygienic status of 33 high school foodservice systems in Yongin city by using hygiene management guide checklist, ATP bioluminescence assay and microbe inspection petrifilm (APC, coliform group, Staphylococcus aureus) of food utensils during use. The 22 hygiene management guide checklist items about facilities, personal hygiene, food control, distribution, washing and disinfection had good grade but there were some inadequate behaviors on observation. The inspection results showed their sanitary condition met the level B of the recommendation of Korea method, it means sanitary management system get settled but more practical CCP system was needed. ATP bioluminescence assay was conducted on surface of food facilities, ATP ranged 425~2,552 RLU on gloves, 541~70,251 RLU on apron, 1,596~88,490 RLU on working desk, 1,177~263,813 RLU on sterilizer grip, 715~32,814 RLU on sterilizer shelf, 114~619,725 RLU on refrigerator grip, 677~319,007 RLU on refrigerator shelf, 71~196,725 RLU on freezer grip, 1,535~233,375 RLU on freezer shelf. APC ranged $66.7{\pm}29.0CFU$ on freezer grip, $102.1{\pm}35.9CFU$ on refrigerator grip, $45.4{\pm}28.2CFU$ on heating cabinet grip, $58.8{\pm}40.4CFU$ on sterilizer grip, the number of coliform group ranged $5.6{\pm}4.9CFU$ on freezer grip, $9.1{\pm}8.7CFU$ on refrigerator grip, $1.2{\pm}1.1CFU$ on heating cabinet grip, $4.5{\pm}4.4CFU$ on sterilizer grip. S. aureus ranged $8.0{\pm}5.6CFU$ on freezer grip, $12.2{\pm}9.6CFU$ on refrigerator grip, $2.1{\pm}1.6CFU$ on heating cabinet grip, $11.6{\pm}6.4CFU$ on sterilizer grip.

White Spot Baculovirus(WSBV) 미감염 새우(Penaeus chinensis, Penaeus japonicus) 종묘 생산 (Production of Healthy Shrimp(Penaeus chinensis, Penaeus japonicus) Seedling Free from White Spot Baculovirus(WSBV))

  • 허문수;손상규;김종식
    • 한국어병학회지
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    • 제14권1호
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    • pp.11-15
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    • 2001
  • White Spot Baculovirus(WSBV) 미감염 새우 건강 종묘를 생산하기 위하여 난의 요오드 소독을 실시하였다. 유효 요오드농도 20,50,100,200 ppm에서 각 시험구의 대하(Penaeus chinensis) 수정난을 각각 30,60초 처리한 결과 각 농도의 30초 처리구에서는 50%이상의 상대부화율을 보였고, 60초 처리구에서는 20ppm 처리구에서만 약 50% 상대 부화율을 보인 반면 50 ppm이상에서는 부화율이 거의 없었다. 보리새우(Penaeus japoni cus)에서도 비슷한 결과를 보였다. 대하의 경우 세란만 실시한 대조구의 부화율은 33.3%이었지만, 보리새우는 55.2%로 상대적으로 높았다. 부화후 생존율을 보면 수정난의 소독으로 부화율은 낮아지지만 수정란 소독후 부화된 유생은 초기생육에는 문제점이 없었다. WSBV 미감염 건강종묘를 생산하기 위해서는 수정난을 세척하고 저농도의 요오드를 짧은 시간에 소독해야 한다.

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치과진료실에서의 직종별 감염방지 실천 정도에 관한 조사 연구 (A Study on Practice of Infection Control among Dental Staffs in Dental Office)

  • 이영애;조민정;배지영;박현숙
    • 치위생과학회지
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    • 제7권4호
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    • pp.263-269
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    • 2007
  • 치과진료실에서의 감염방지 실태를 분석하여 향후 치과진료실에서의 감염방지 수준을 향상시키기 위한 방안을 제시하고자 대구광역시 소재 치과 병 의원에 근무하는 치과의사 50명, 치과위생사 176명, 간호조무사 100명을 대상으로 감염방지 교육 및 감염성 관리 실태를 분석한 결과는 다음과 같다. 1. 건강검진 실시율은 치과의사가 가장 높았고, 예방접종 실시율은 치과위생사가 가장 높았다. 2. 감염방지 교육 경험률은 치과의사가 가장 높았고, 간호조무사가 가장 낮았다(P < 0.05). 3. 개인용 보호장비 착용률은 치과의사가 가장 높았고, 간호조무사가 가장 낮았다(P < 0.05). 4. 진료 후 손 세척 시행률은 치과의사가 가장 높게 나타났다(P < 0.05). 5. 기구의 멸균 및 소독 시행률은 치과위생사가 다른 두 집단에 비해 높았다. 6. 장비의 표면 소독은 진료용 선반 위주로 시행하고 있는 것으로 나타났으며, 치과위생사 의 실천률이 높았다.

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영양부추 생산농가의 소규모 수확후 처리시설 적용과 위생교육에 따른 미생물학적 안전성 향상 효과 (Effects of Small Scale Post-Harvest Facility and Hygiene Education on the Level of Microbial Safety in Korean Leeks Production)

  • 김세리;김진배;이효섭;이은선;김원일;류송희;하지형;김황용;류재기
    • 한국식품위생안전성학회지
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    • 제30권3호
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    • pp.249-257
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    • 2015
  • 위생적인 수확후처리를 통하여 안전한 농산물 생산을 유도하기 위하여 영양부추를 대상으로 수확후 처리시설 모델을 개발하였으며 개발된 수확후 처리시설 설치와 위생교육이 미생물 안전에 미치는 효과를 검증하고자 본 연구를 수행하였다. 이를 위하여 양주지역 영양부추 생산 농가의 수확 후 처리시설 환경과 영양부추에서 위생지표세균(일반세균수, 대장균군, E. coli)과 병원성미생물(Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus)을 조사하였다. 그 결과, 빗, 칼, 도마 등 수확후 처리시설에서 사용하는 작업도구의 일반세균수 오염수준은 수확후 처리시설 설치농가(A, B)에서 수확후 처리시설 비설치농가(C) 보다 1.44~2.33 log CFU / 100$100cm^2$ 정도 낮았다. 특히 도마의 경우 A농가에서 1.00 log CFU / 100$100cm^2$이하, B 농가에서 2.23 log CFU / $100cm^2$인데 반해 C 농가에서는 6.03 log CFU / $100cm^2$로 농가간의 위생 상태에 따라 B. cereus의 오염수준이 차이가 크게 나타났다. 또한 지하수에 침지 한 영양부추에서 침지 전 보다 대장균군이 0.57~1.89 log CFU/g이 증가하였다. E. coli는 영양부추, 침지한 후 지하수, 토양에서 검출되었으며, E. coli O157:H7, Salmonella spp., L. monocytogenes는 검출되지 않았다. 따라서 본 연구의 결과를 통하여 소규모 수확 후 처리시설 설치와 위생교육을 통하여 농가 내 수확후처리 환경의 위생을 개선하는데 효과적이라 판단된다. 이와 더불어 유해미생물에 의한 식중독사고를 사전에 예방하기 위해서는 수확 후에 오염된 유해미생물을 저감화 할 수 있는 세척, 소독 기술의 개발과 도입이 필요할 것으로 사료된다.

전북지역 초중고등학교 학교급식소의 내부시설 및 위생관리 실태 조사 (Survey on Internal Facilities and Sanitary Management of Elementary, Middle, and High School Foodservice in Chonbuk Area of Korea)

  • 국숙자;노정옥
    • 한국생활과학회지
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    • 제18권5호
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    • pp.1135-1145
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    • 2009
  • This study was conducted to investigate the situation of internal facilities and sanitary management of elementary, middle, and high school foodservice in Chonbuk area. Self administered questionnaires were collected from 252 nutrition teachers and school dietitians. Statistical data analysis was completed using a SPSS 11.5 program. The results are summarized as follows: Approximately 99.2% of the subjects were women and those who were married accounted for 76.6%. Those in their 30s to 40s accounted for over 87%, those with more than 11 years experience accounted for 56.8%, and those 69.0% were regular nutrition teachers. Among the 280 school foodservice systems, 51.6% of the schools were located in urban areas and 48.0% in rural areas. About 68% of the schools prepared meals the conventional way and 32.3% prepared them the commissary way. The number of employees accounted for in the schools was 37.7% with 1 to 3 employees, 27.8% with 4 to 6 employees, and 25.4% with 7 to 9 employees. About 54% of schools have an expectance of school meal service for more than 11 years. However 67.5% of their facilities had not been remodeled since the implementation of the foodservice. As a result of the conditions of the school foodservice facilities, there were significant differences between elementary and middle & high schools; preparation room (p<0.01), toilet (p<0.01), and boiler room (p<0.01). About 62% of the nutrition teachers responded that they were dissatisfied with the situation of the facilities (e.g. size and materials of the kitchen, heating and cooling systems). The washing and disinfection methods in elementary, middle and high schools were very different, because they had no sanitizers and disinfectant guidelines. Therefore the governmental regulating agencies must review and approve of the plans prior to new construction or extensive remodeling of the school foodservice facilities. In addition, we suggest that it is necessary to provide practical sanitizers and disinfectant guidelines and other useful education.