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$^{31}p$ Nuclear Magnetic Resonance Studies of Acetic Acid Inhibition of Ethanol Production by Strains of Zymomonas mobilis

  • Kim, In-Seop;Barrow, Kevin D.;Rogers, Peter L.
    • Journal of Microbiology and Biotechnology
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    • v.13 no.1
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    • pp.90-98
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    • 2003
  • In vivo $^31p$ Nuclear Magnetic Resonance ($^31p$NMR) and metabolic studies were carried out on an acetic acid tolerant mutant, Zymomonas mobilis $ZM4/Ac^R$, and compared to those of the parent strain, Z. mobilis ZM4, to evaluate possible mechanisms of acetic acid resistance. This investigation was initiated to determine whether or not the mutant strain might be used as a suitable recombinant host far ethanol production from lignocellulose hydrolysates containing various inhibitory compounds. $ZM4/Ac^R$ showed multiple resistance to other lignocellulosic toxic compounds such as syringaldehyde, furfural, hydroxymethyl furfural, vanillin, and vanillic acid. The mutant strain was resistant to higher concentrations of ethanol or lower pH in the presence of sodium acetate, compared to ZM4 which showed more additive inhibition. in vivo $^31p$ NMR studies revealed that intracellular acidification and de-energization were two mechanisms by which acetic acid exerted its inhibitory effect. For $ZM4/Ac^R$, the internal pH and the energy status were less affected by sodium acetate compared to the parent strain. This resistance to pH change and de-energization caused by acetic acid is a possible explanation for the development of resistance by this strain.

Consumer Perceptions and Intentions Towards Malaysian Mobile Marketing

  • Chee, Sua Wui;Yee, Woo Kuan;Saudi, Mohd Haizam Mohd
    • Asian Journal of Innovation and Policy
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    • v.7 no.2
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    • pp.338-363
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    • 2018
  • Mobile marketing is a brand new phenomenon. In Malaysia, marketeers do not seem to understand well enough the perceptions of consumers according to mobile marketing, especially to the mobile service users. This study analyses and evaluates any significant relationship between consumer perceptions and intentions with respect to mobile marketing, and seeks to determine the expectations, preferences, pattern and usage of the consumers of Mobile Marketing Product and Service (MMP&S). The data was obtained by convenience sampling in the big cities of Malaysia. A total of 500 questionnaires were distributed and a sample of 112 usable questionnaires was selected. The result of this research applying the Technology Acceptance Model (TAM) point to the fact that perceived usefulness, perceived ease of use, perceived innovative, social influence have a direct positive relationship with the intention to use mobile marketing. This, in turn, can shed light on the main factors determining consumer intentions to use mobile marketing that may control consumer adoption of mobile marketing.

The Effects of Mobile Learning Factors and Training Transfer on the Effective Organisational Learning in Malaysian Oil and Gas Industry

  • Chee, Sua Wui;Saudi, Mohd Haizam Mohd;Lee, Chong Aik
    • Asian Journal of Innovation and Policy
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    • v.7 no.2
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    • pp.310-337
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    • 2018
  • Adoption of mobile learning (m-learning) is not new in Malaysian oil and gas industry, with heavy investment into research and development to train the workers. Nevertheless, the low application of learnt skills on the job remains an emergent research area where there is a missing link on the effects of m-learning and effective organisational learning and implication on its training transfer. The result of this quantitative research revealed that all variables in m-learning were found to have a positive relationship with the effective organisational learning, and there is evidence of training transfer as a mediator of the relationship between self-directed learning, training design, work environment and effective organisational learning. However, there were some discrepancies in the extend of training transfer between trainee characteristics and organisational learning. As such, some important issues emerged which challenge the importance of evaluating workers' readiness and transfer for a successful implementation of m-learning towards developing effective organisational learning.

A Study on the Dimensional Characteristics of the Weft Knitted Fabrics with Bamboo Knitted Yarn (Bamboo사 평 편조직 위편성물의 형태안정성에 관한 연구)

  • Choi, Jae-Woo;Jang, Bong-Sik;Lee, Eun-Woo;Kim, Dong-Yeub
    • Journal of the Korean Society of Industry Convergence
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    • v.15 no.3
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    • pp.79-82
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    • 2012
  • Aim of this study is to investigate the dimensional characteristics of the weft knitted fabrics with Bamboo knitted yarn. This Bamboo knitted yarn was made of the biodegradability fiber. The structure of weft knitted fabrics that was utilized for this study is the plain stitch, which is the most basic structure among all weft knitted fabrics. The loop density, courses density, and wales density are more increased as the loop length is shorter. The loop density increased as pre-treatment process and dyeing process progressed after dry treatment process, and on the contrary, the heat setting process made it decreasing. After the dyeing process proceed, the loop density and the course density were displayed the highest values. When the knitted fabrics were fully relaxed, the loop density was $2000/in^2$, the courses density was 52/in, the wales density was 39/in.

Research on Traditional Chinese Medicine harmonising two approaches

  • Chung, Leung Ping;Wai, Lau Tai;Sang, Woo Kam
    • Advances in Traditional Medicine
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    • v.8 no.1
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    • pp.17-23
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    • 2008
  • While full recognition of the practical value of Traditional Chinese Medicine is being endorsed, the current stand on the research methodology of this field should be worked out. Since modern medicine has already developed a logical system of research methodology basing on the principles of deduction, any research on any system of medicine need to take reference to what is most popularly used and commonly recommended. The best way to approach research on Chinese Medicine, therefore, would be one that would take full reference to the methodology being used in modern medicine, while at the same time respecting the traditional approach. This would enable traditional medicine to be elevated to the level of general modern recognition. Nevertheless, innate problems in traditional medicine are making its research difficult. The problems lie in difficulties to achieve uniform herb supply, principles of randomization and placebo arrangements, uncertain chemical structures and toxicology etc. A practical approach centered on carefully planned evidence-based clinical trials, with parallel studies on biological activities and herb authentication is being recommended.

Reinforced concrete beams under drop-weight impact loads

  • May, Ian M.;Chen, Yi;Owen, D. Roger J.;Feng, Y.T.;Thiele, Philip J.
    • Computers and Concrete
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    • v.3 no.2_3
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    • pp.79-90
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    • 2006
  • This paper describes the results of an investigation into high mass-low velocity impact behaviour of reinforced concrete beams. Tests have been conducted on fifteen 2.7 m or 1.5 m span beams under drop-weight loads. A high-speed video camera has been used at rates of up to 4,500 frames per second in order to record the crack formation, propagation, particle spallation and scabbing. In some tests the strain in the reinforcement has been recorded using "Durham" strain gauged bars, a technique developed by Scott and Marchand (2000) in which the strain gauges are embedded in the bars, so that the strains in the reinforcement can be recorded without affecting the bond between the concrete and the reinforcement. The impact force acting on the beams has been measured using a load cell placed within the impactor. A high-speed data logging system has been used to record the impact load, strains, accelerations, etc., so that time histories can be obtained. This research has led to the development of computational techniques based on combined continuum/discontinuum methods (finite/discrete element methods) to permit the simulation of impact loaded reinforced concrete beams. The implementation has been within the software package ELFEN (2004). Beams, similar to those tested, have been analysed using ELFEN a good agreement has been obtained for both the load-time histories and the crack patterns.

HOW DO MASSIVE STARS FORM? INFALL & OUTFLOW IN DENSE CORES IN THE MILKY WAY

  • AKHTER, SHAILA.;CUNNINGHAM, MARIA R.;HARVEY-SMITH, LISA;JONES, PAUL A.
    • Publications of The Korean Astronomical Society
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    • v.30 no.2
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    • pp.99-101
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    • 2015
  • Massive stars are some of the most influential objects in the Universe, shaping the evolution of galaxies, creating chemical elements and hence shaping the evolution of the Universe. However, the processes by which they form and how they shape their environment during their birth processes are not well understood. We use $NH_3$ data from "The $H_2O$ Southern Galactic Plane Survey" (HOPS) survey to define the positions of dense cores/clumps of gas in the southern Galactic plane that are likely to form stars. Then, using data from "The Millimetre Astronomy Legacy Team 90 GHz" (MALT90) survey, we search for the presence of infall and outflow associated with these cores. We subsequently use the "3D Molecular Line Radiative Transfer Code" (MOLLIE) to constrain properties of the infall and outflow, such as velocity and mass flow. The aim of the project is to determine how common infall and outflow are in star forming cores, and therefore to provide valuable constraints on the timescales and physical process involved in massive star formation. Preliminary results are presented here.

Improving Covariance Based Adaptive Estimation for GPS/INS Integration

  • Ding, Weidong;Wang, Jinling;Rizos, Chris
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • v.1
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    • pp.259-264
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    • 2006
  • It is well known that the uncertainty of the covariance parameters of the process noise (Q) and the observation errors (R) has a significant impact on Kalman filtering performance. Q and R influence the weight that the filter applies between the existing process information and the latest measurements. Errors in any of them may result in the filter being suboptimal or even cause it to diverge. The conventional way of determining Q and R requires good a priori knowledge of the process noises and measurement errors, which normally comes from intensive empirical analysis. Many adaptive methods have been developed to overcome the conventional Kalman filter's limitations. Starting from covariance matching principles, an innovative adaptive process noise scaling algorithm has been proposed in this paper. Without artificial or empirical parameters to be set, the proposed adaptive mechanism drives the filter autonomously to the optimal mode. The proposed algorithm has been tested using road test data, showing significant improvements to filtering performance.

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Comparison of evolutionary organic farming policies reflected on the first and second Wales and England organic action plans (OAPs) for Korean context - As a tool ensuring the sustainable development of Korean organic farming

  • Cho, Y.;Nicholas, P.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.78-81
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    • 2011
  • This study compares and contrasts the Wales and England Organic Action Plans (WOAP and EOAP) and a Korean regional Life-Food Development Plan (KLFDP) in order to facilitate the development of the organic sector in Korea. Early action plans, for example, the first WOAP (1999) focused support on developing the supply of organic products whereas later action plans focused on marketing and consumer. OAPs may not only provide specific issue-solving roles by proposing new policy measures but also perform a regulatory role as a controller for organic sector development as a whole. The current KLFDP seems to stick to the former role but hardly has the latter role such as setting priorities, harmonizing various conflicting policy measures and factors and performing evaluation process for further progress. To secure better harmonized and sustainable development of Korean organic farming sector, constructing comprehensive national-level organic action plan, which has policy developing, implementing, regulating, evaluating and evolving functions, might be the most efficient choice.

Optimization approach applied to nonlinear analysis of raft-pile foundations

  • Tandjiria, V.;Valliappan, S.;Khalili, N.
    • Structural Engineering and Mechanics
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    • v.7 no.6
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    • pp.533-550
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    • 1999
  • Optimal design of raft-pile foundations is examined by combining finite element technique and the optimization approach. The piles and soil medium are modeled by three dimensional solid elements while the raft is modelled by shell elements. Drucker-Prager criterion is adopted for the soil medium while the raft and the piles are assumed to be linear elastic. For the optimization process, the approximate semi-analytical method is used for calculating constraint sensitivities and a constraint approximation method which is a combination of the extended Bi-point approximation and Lagrangian polynomial approximation is used for predicting the behaviour of the constraints. The objective function of the problem is the volume of materials of the foundation while the design variables are raft thickness, pile length and pile spacing. The generalized reduced gradient algorithm is chosen for solving the optimization process. It is demonstrated that the method proposed in this study is promising for obtaining optimal design of raft-pile foundations without carrying out a large number of analyses. The results are also compared with those obtained from the previous study in which linear analysis was carried out.