• Journal of Korean Society of Forest Science
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• v.80 no.4
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• pp.416-419
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• 1991

Effective micropropagation was achieved by axillary bud culture from stool shoots of 15-year-old Betula platyphylla var. japonica. Shoot development and proliferation from the explants were successful on WPM supplemented with 0.5 or 1.0mg/l BAP. All the regenerated shoots rooted when transfered to GD medium containing 0.2mg/l IBA. After transplaning to soil more than 95% of the plantlets survived and showed normal growth. The results demonstrate that masspropagation of selected mature trees is feasible using tissue culture technique.

• Korean Journal of Agricultural Science
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• v.14 no.2
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• pp.205-212
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• 1987

To examine the morphogenetic response, stem segments of 8 Populus spp. and 3 different explants of P. nigra var. italica were cultured on MS (Murashige and Skoog 1962) and WPM (Woody Plant Medium) medium containing various phytohormones. The results obtained were as follows: 1. Shoot regeneration and development from stem segment of 8 Populus spp. showed a quite difference according to the section and the species. All of the species of Leuce and Tacamahaca section did not form adventitious buds, while most of explants showed axillary or dormant bud elongation after 4 weeks. But P. nigra var. italica of Aigeiros section showed a successful adventitious bud formation (mean 5.4 buds per explant). 2. Leaf, petiole, and internode segment of P. nigra var. italica showed a quite differences according to media and ex plants upon the morphogenetic response. Adventitious bud formation from leaf was more abundant and readily initiated on the abaxial side than on the adaxial side. Mean number of 103 adventitious buds per explant was obtained from abaxial side of leaf segment cultured on WPM medium containing $0.2mg/{\ell}$ BAP for 5 weeks. 3. 2,4-D (2,4-dichlorophenoxy acetic acid) supplemented to media appeared to be negative upon the adventitious bud formation of P. nigra var. italica, while it promoted callus formation from all explants. Especially, NAA (${\alpha}$-naphtalene acetic acid) or NAA combination with BAP (6-benzylaminopurine) promoted root regeneration from the all explant of P. nigra var. italica in this study.

• Journal of Korean Society of Forest Science
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• v.96 no.1
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• pp.83-88
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• 2007

Micropropagation of Ulmus davidiana Planch was established via adventitious shoot formation from the segments of adventitious roots. Adventitious roots were produced directly from root segments of seedlings on a 1/2 SH medium plus various concentrations of IBA. The maximum growth of adventitious roots was observed in the presence of 2.0 mg/L IBA. After the segments of adventitious roots were cultured on various cytokinins (zeatin, 2-iP, BA, kinetin) and cytokinins plus auxin (IBA), formation of adventitious shoot was investigated. Among cytokinin treated, kinetin was the most effective on both adventitious shoot induction and number of shoots. Especially, 2.0 mg/L kinetin was the best to increase adventitious shoot induction (95.8%) and a number of shoots (8.4). Adventitious shoots were rooted on 1/2 WPM medium and the plantlets were acclimated 100% on composed soil (peatmoss : vermiculite 1 : 1).

• Broadcasting and Media Magazine
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• v.11 no.1
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• pp.86-92
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• 2006

점차 사용 가능한 대역이 포화됨에 따라 이미 할당된 스펙트럼 자원을 효율적으로 공유하고자 하는 무선인지(cognitive radio) 기술이 대역포화 문제를 해결할 수 있는 주요한 기술로 등장하고있다. 이 원고에서는 광대역 무선인지 기술에 필요한 물리계층에서의 필요조건을 살펴보고 이에 적합한 변조방식에 대해 살펴본다. 특히 광대역 무선인지 기술에 필요한 유연성을 제공할 수 있고 인접채널 간섭을 크게 감소시킬 수 있는 웨이블릿 패킷 변조(wavelet packet modulation: WPM) 기술에 대해 소개한다.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.380-387
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• 2015

Aronia (Aronia melanocarpa, Black chokeberry) is an important cash crop in domestic agriculture. We investigated the effects of plant growth regulators on shoot proliferation and rooting using in vitro tissue culture. The most effective shoot multiplication was observed on WPM (woody plant medium) supplemented with 1.0 mg/L zeatin ($8.3{\pm}1.0$ shoots/explant), while the highest rooting rate was obtained from half-strength WPM with 3.0 mg/L IBA (8.8 roots/explant). The rooted plantlets all survived in the artificial soil mixture (with a mixture of peat moss : perlite : vermiculite, 1:1:1, v/v/v) and grew up relatively uniform, ranging from 14 to 16 leaves, 8 to 10 cm in stem height, and 2.3 to 2.8 mm in stem diameter. While experimenting with 5 different varieties of Aronia, we found out that each variety had different characteristics of shoot proliferation and rooting. The total numbers of proliferated shoots per variety is as follows: $17.4{\pm}0.8$ for Nero, 14 to 15 for Purple and Mackenzie, and 10 for both Viking and Odamamachiko. Rooting rates were also various depending on the variety: 88% of Odamamachiko, 80% of Viking and Purple, and 76% of Nero and 60% of Mackenzie shoots rooted. The survival rate of the rooted plantlets was from 92% to 100%, varying by type. Further growth appeared to be better in auxin-treated plantlets, compared to untreated ones. Our results showed the possibility of establishing an effective in vitro micropropagation system for Aronia melanocarpa.

• Journal of Korean Society of Forest Science
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• v.98 no.6
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• pp.719-725
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• 2009

This study was conducted to establish the optimal condition for acclimatization from somatic embryos of Eleutherococcus seoulensis. Torpedo-shaped embryos of Eleutherococcus seoulensis were cultured on 1/3 MS and WPM media supplemented with $GA_3$ (3.0, 5.0 mg/L) for 4 weeks. Plentlets were transferred to 1/2 SH solid medium with 1.0 mg/L $GA_3$ and 0.2% activated charcoal for shoot and root elongation and them elongated plantlets further developed on 1/2 SH medium for 4 weeks. Developed plantlets further elongated into well-shaped leaf and root system on 1/3 SH medium under ventilation condition for 4 weeks. Plantlets grew normally on 1/3 SH basal medium, were acclimated on various soil. Survival frequency of plantlets was influenced by soil type(peatmoss+perlite, perlite, soil on Nam mountain). The highest survival rate to soil was more than 70% when plantlets were 1/3 SH medium under ventilation condition in Nam mountain soil. These results indicate that the systematic procedure of plant production in Eleutherococcus seoulensis could be practically applicable for mass propagation.

• Korean Journal of Medicinal Crop Science
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• v.10 no.2
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• pp.88-92
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• 2002

The production of anthraquinone has been dectected in transformed roots of Rheum undulatum L. The effects of medium, initial pH, concentration of sucrose, light irradiation and elicitors on anthraquinone production in transformed roots of Rheum undulatum L. were investigated. The maximum production of anthaquinone was achieved in WPM medium (pH 5.7) supplemented with 6% sucrose, 0.5 mg/l $GA_3$, and 50 mg/l chitosan at 16h light $(16{\mu}mol\;m^{-2}s^{-1})$ condition. Under the optimum conditions, the production of anthraquinone reached to 0.18 mg/g(F.W.) after 8 weeks. The content was estimated about 1.3 times of the level of native roots.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.263-268
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• 1997

Effects of plant growth regulators and condition on shoot induction from internode explants of Actinidia arguta were investigated. Optimal condition for shoot induction was obtained when internode explants were cultured on MS medium containing 3.0 mg/L zeatin. The frequency showed about 62.5% under darks condition at 27$^{\circ}C$ after 6 weeks of culture. Regenerated shoots were rooted on WPM medium supplemented with 0.5 mg/L NAA and the regenerants were acclimated to an artificial soil with mixture (perlite : vermiculite = 1:1, v/v) for further growth and successfully transplanted to the soil in a highly humidified greenhouse. Histological observations revealed that meristemoids were formed from small and isodiametric cells, and then developed to shoot primordia.

• Journal of the Ergonomics Society of Korea
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• v.33 no.6
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• pp.499-513
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• 2014

Objective: The objective of this study was to examine frame ratio of text information and font size in the circle smartwatch. Background: Recently, electronic manufacturers try to develop the original metaphor of traditional wrist watch (circle) in terms of smartwatch. They endeavor to break the square display in order to improve emotional customer satisfaction. Method: The experiments examined twenty level of text information design, combinations of four frame ratios (1:1, 4:3, 16:9, 21:9) and five font sizes (6pt, 7pt, 8pt, 9pt, 10pt). Nineteen participants volunteered for the experiment. Dependent variables were WPM (Words per Minute), reading preference, design preference and total preference. Furthermore, small circle display was made by using circle display data (1.3inch), which was exhibited in IFA (International Funkausstellung) 2014. Results: As a result, ANOVA (Analysis of Variance) revealed that WPM, and task time preference affect the specific frame ratio and font size. Results of ANOVA for reading preference, design preference, total preference were grouped by post-analysis LSD (Least Significant Difference). Among users, display ratio (16:9, 21:9), and font size (9pt) were preferred. In conclusion, 16:9 display ratio and 9pt are adaptable for text information in 1.3inch circle display. Conclusion: From the study, it is shown that 16:9 display ratio and 9pt size are more adaptable for text information in 1.3inch circle display than others. It is mainly due to the fact that the order of frame ratio and font size may affect the usability of reading long text information in a small circle display. Therefore, when developers design a circle display, the square frame ratio and font size are required to be considered according to circle size. Application: The 16:9 display ratio and 9pt font size may be utilized as a text information frame in the circle display design guideline for smartwatch.

• Journal of Forest and Environmental Science
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• v.37 no.4
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• pp.323-330
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• 2021

Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA₃ on the growth of plants germinated from zygotic embryos through GA₃ treatment, the best growth was shown in 0.5 mg/l GA₃ treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.