• Annual Conference of KIPS
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• 2000.10b
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• pp.1513-1516
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• 2000

인터넷 사용자의 비약적인 증가와 발전에 따라 교육공학에 있어서도 구성주의를 기반으로 한 웹 기반 학습의 역할이 매우 중요한 하나의 교육 분야로서 인식되고 있다. 따라서 웹을 기반으로 한 수많은 학습 사이트가 등장하고 있으며, 이를 이론적으로 정립하기 위한 연구도 계속되고 있다. 본 논문에서는 구성주의 및 웹 기반 학습 이론에 충실하면서, 구조적이고 학습 효과를 높일 수 있는 웹 기반 학습[WBL] 환경을 구현하기 위하여, 기존의 웹 기반 학습 사이트에 대한 분석을 바탕으로 각 모듈 사이에 유기적인 관계를 가지고 학습자의 학습을 관리해 줌으로써, 학습 효과를 최대한 높일 수 있는 웹 기반 학습 환경을 구현하였다. 또한 UML 방법론을 사용하여 재사용 가능한 메시징 컴포넌트를 구현 및 이용함으로써, 웹 환경에서 교사-학습자간, 학습자-학습자간의 적극적인 상호작용을 할 수 있고, 다른 웹 기반 학습 사이트에서도 재사용 할 수 있도록 하였다.

• Microbiology and Biotechnology Letters
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• v.40 no.1
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• pp.10-16
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• 2012

The Streptomyces peucetius OIM (Overproducing Industrial Mutant) strain is a recursively-mutated and optimally-screened strain used for the industrial production of polyketide antibiotics, such as doxorubicin (DXR). Using the S. peucetius OIM mutant strain as a surrogate host, a model minimal polyketide pathway for aloesaponarin II, an actinorhodin shunt product, was cloned in a high-copy conjugative plasmid, followed by functional pathway expression and quantitative metabolite analysis. The level of aloesaponarin II production was noted as being significantly higher in the OIM strain than in the wild-type S. peucetius, as well as in the regulatory network-stimulated S. coelicolor mutant strain. Moreover, the aloesaponarin II production level was seen to be even higher in a down-regulator $wblA_{spe}$-deleted S. peucetius OIM strain, implying that the rationally-engineered S. peucetius OIM mutant strain could be used as an efficient surrogate host for the high expression of foreign polyketide pathways.

• Journal of Microbiology and Biotechnology
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• v.33 no.11
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• pp.1531-1541
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• 2023

Lipase is a well-known and highly in-demand enzyme. During the last decade, several lipase optimization studies have been reported. However, production costs have always been a bottleneck for commercial-scale microbial enzyme production. This research aimed to optimize the conditions for lipase production by Limtongozyma siamensis DMKU-WBL1-3 via a One-Factor-At-a-Time (OFAT) approach combined with statistical methods while using a low-cost substrate. Results suggest that low-cost substrates can be substituted for all media components. An optimal medium was found, using response surface methodology (RSM) and central composite design (CCD), to consist of 0.50% (w/v) sweet whey, 0.40% (w/v) yeast extract (food grade), and 2.50% (v/v) palm oil with the medium pH adjusted to 4 under shaking flask cultivation. From an economic point of view, this work was successful in reducing production costs while increasing lipase productivity. The medium costs were reduced by 87.5% of the original cost while lipase activity was increased by nearly 6-fold. Moreover, lipase production was further studied in a 2-L stirred-tank fermentor. Its activity was 1,055.6 ± 0.0 U/ml when aeration and agitation rates were adjusted to 1 vvm and 170 rpm, respectively. Interestingly, under this optimal lipase production, the yeast showed accumulated lipids inside the cells. The primary fatty acid is a monounsaturated fatty acid (MUFA) that is typically linked to health benefits. This study hence reveals promising lipase production and lipid accumulation by L. siamensis DMKU-WBL1-3 that are worthy of further study.

• Proceedings of the KSR Conference
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• 2004.06a
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• pp.767-772
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• 2004

A dynamic simulation program of a catenary-pantograph system including tunnel section and transient section is developed in this study. The simulation program can accommodate for the pantograph of two panheads and three d.o.f model. Using the developed program, the dynamic characteristics with a SCHUNK'S WBL 85-PANTOGRAPH is analyzed at the conventional TAEBAEK line and its tunnel section when the catenary system is supported by a tunnel bracket. The simulation results show that the variation of contact force and uplift displacement is allowable in general section and the entrance and exit of a tunnel, but the uplift displacement and the separation ratio within tunnel section is difficult to allow.

• Proceedings of the KAIS Fall Conference
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• 2010.05b
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• pp.981-984
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• 2010

생산성 증가 및 비용 절감을 위해 반도체 공정 기술을 단순화 시키는 것이 필요하다. WBL(Wafer Backside Lamination) 기술을 이용해 필름(film) 형태로 얇은 다이접착제를 웨이퍼(wafer)에 접착하여 반도체 칩과 PCB를 붙이는 방법과 직접 PCB에 다이접착제를 붙이는 방법을 사용하면 획기적으로 공정을 단순화 시킬 수 있다. 하지만 Lamination 기법은 고온을 이용하여 모듈화된 PCB에 접착하므로 전도와 복사에 의해 주변 접착제 필름이 녹아 버리는 문제점이 발생한다. 본 연구에서는 고온으로 인한 필름 융해 현상을 방지하기 위하여 배크라이트를 설치하였으며 CFD 해석을 통해 PCB와 반도체 칩을 접착시킬 때 열이 PCB에 미치는 영향을 살펴보았다.

• Journal of the Korean Society for Railway
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• v.8 no.1
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• pp.51-56
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• 2005

A dynamic simulation program of a catenary-pantograph system including tunnel section and transient section is developed in this study. The simulation program can accommodate for the pantograph of two panheads and three d.o.f model. Using the developed program, the dynamic characteristics with a SCHUNK'S WBL 85-PANTOGRAPH are analyzed at the conventional TAEBAEK line and its tunnel section when the catenary system is supported by a tunnel bracket. The simulation results show that the variation of contact force md uplift displacement is allowable in general section and the entrance and exit of a tunnel, but the uplift displacement and the separation ratio within tunnel section is difficult to allow.

• Korean Journal of Food Science and Technology
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• v.50 no.6
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• pp.608-613
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• 2018

For increasing both the viscosity and intake of calories and nutrients, four cereal powders: Goami (GR), waxy barley (WBL), germinated brown rice (GBR), and oat (OT) were added to rice porridge, and the properties of the porridge were compared with that containing a commercial thickener. Among the samples, the porridge with WBL showed the highest viscosity, whereas that with GR showed the lowest. With regards to color properties, the porridge with GR showed the highest whiteness, while those with OT or GBR had the highest yellowness, affecting the color of the rice porridge. The viscosity level of porridges with cereal powders did not reach that of the porridge with the commercial thickener at $90-95^{\circ}C$; however, at $55-60^{\circ}C$, the viscosity of the porridge with cereals was enough to replace the commercial thickener. With regards to organoleptic tests, the overall acceptability was mainly attributed to color, and there were no significant differences in other properties. From these results, it seems that the cereal powders could be suitable substitutes for commercial thickeners.

• Child Health Nursing Research
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• v.11 no.1
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• pp.117-124
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• 2005

Purpose: The purpose of this study was to investigate the effects of sensory stimulation conducted by primiparas on mother-infant interaction, maternal self-esteem and infant temperament. Method: A non-equivalent control group post-test study design was used. The data were collected from December 1, 2001 to March 30, 2003. The participants were 39 primiparas with normal birth weight infants. They were divided into two groups, 20 mothers in the intervention group and 19 in the control group. The mothers in the intervention group were given instruction on sensory stimulation by the researcher, and the mothers, in turn, used this technique on their infants twice a day during the first 2 months of life. Both groups were measured for maternal self-esteem and infant temperament using the maternal self-report inventory (MSRI) and What My Baby Is Like (WBL) and a film was made of the feeding situation when each infant was between 8 and 10 weeks of age. Results: Compared to the control group, the experimental group had significantly higher scores in mother-infant interaction, maternal self-concept and infant temperament. Conclusion: This suggests that sensory stimulation conducted by primiparas may improve the mother-infant interaction, maternal self-esteem and infant temperament.

• The Plant Pathology Journal
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• v.36 no.2
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• pp.185-191
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• 2020

Streptomyces griseus S4-7, a well-characterized keystone taxon among strawberry microbial communities, shows exceptional disease-preventing ability. The whole-genome sequence, functional genes, and bioactive secondary metabolites of the strain have been described in previous studies. However, proteomics studies of not only the S4-7 strain, but also the Streptomyces genus as a whole, remain limited to date. Therefore, in the present study, we created a proteomics reference map for S. griseus S4-7. Additionally, analysis of differentially expressed proteins was performed against a wblE2 mutant, which was deficient in spore chain development and did not express an antifungal activity-regulatory transcription factor. We believe that our data provide a foundation for further in-depth studies of functional keystone taxa of the phytobiome and elucidation of the mechanisms underlying plant-microbe interactions, especially those involving the Streptomyces genus.

• Water for future
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• v.10 no.1
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• pp.79-94
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• 1977

This study is aimed at the establishment and examination of stochastic model to simulate Run-length and Run-sum of daily rainfall and streamflow. In the analysis, daily rainfall records in major cities (Seoul, Kangnung, Taegu, Kwangju, Busan, and Cheju) and daily streamflow records of Major rivers (Han, Nakdong and Geum River) were used. Also, the fitness of daily rainfall and streamflow to Weibull and one parameter exponential distribution was tested by Chi-square and Kolmogorov-Smirnov test, from which it was found that daily rainfall and streamflow generally fit well to exponential type distribution function. The Run-length and Run-sum were simulated by the Weibull Model (WBL Model), one parameter exponential model (EXP-1 Model) based on the Nonte Carlo technique. In this result, Run-length of rainfall was fitted for one parameter exponential model and Run-length of streamflow was fitted for Weibull model. And Run-sum of rainfall and streamflow were fit comparatively for regression model. Hereby, statistical charactristics of Simulation data were sinilar to historical data.