• Journal of Korean Physical Therapy Science
• /
• v.1 no.2
• /
• pp.289-299
• /
• 1994

This study was researched the general characteristics, the elbow-knee joint form, the varus and valgus angle and the both knee joint distance of the 215 persons form July 1, 1994 to July 15, 1994. The results were as follows : 1. The form of elbow joint was significant statistically in the sex group(p<0.05). 2. The angle of elbow joint was very significant statistically in the age group and sex. B.M.I. group(p<0.001, p<0.01, p<0.01), and the average angle was $9^{\circ}.51{\pm}4^{\circ}.79$(n=215). 3. The angle of Cubitus valgus was very significant statistically in the age group(p<0.001), and the average angle of Cubitus valgus was $10^{\circ}.85{\pm}3^{\circ}.76$(n=187). 4. The angle of Knee joint was significant statistically in the age group(p<0.05), and the its average angle was $7^{\circ}.63{\pm}4^{\circ}.52$(n=215). 5. The angle of Genu varus was significant statistically in the age and sex group(p<0.05, p<0.05), and the its average was $6^{\circ}.92{\pm}2^{\circ}.36$(n=27). 6. The angle of Genu valgus was very significant statistically in the age group(p<0.001), and the its average angle was $9^{\circ}.75{\pm}2^{\circ}.94$(n=149). 7. The distance of both Knee joint was significant statistically in the age group and B. M. I. group (p<0.001, p<0.05), and the its average distance was $2.55{\pm}1.45cm$(n=135).

• Microbiology and Biotechnology Letters
• /
• v.23 no.2
• /
• pp.164-169
• /
• 1995

An endogenous cryptic plasmid, pBL1, which has been used to construct plasmid vectors for coryneform bacteria producing amino acids, was eliminated from Brevibacterium lactofermentum. The pBL1 was partially digested with Sau3AI and the resulting DNA fragments were subcloned into a suicide vector pEM1 which contains a kanamycin-resistant (km$^{r}$) gene. KM$^{r}$ B. lactofermentum transconjugants were obtained by conjugal transfer of the pEM1 derivatives containing pBL1 DNA fragments from Escherichia coli into B. lactofermentum. A km$^{r}$ transconjugant was analyzed to contain a plasmid pEB14, which occurred in vivo by homologous recombination between pBL1 and the conjugal-transferred plasmid. The pEB14 including the pEM1-derived km$^{r}$ gene was found to be lost concomitantly with km$^{r}$ phenotype, resulting in the construction of a pBL1-free strain of B lactofermentum. Based on transformation efficiencies and plasmid stability, the resultant pBL1- free strain is more useful than wild strain as a host cell for genetic manipulation. It could be concluded that foreign plasmid DNAs are efficiently isolated and analyzed from the pBL1-free strain because of the absence of endogenous pBL1 plasmid.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.21 no.11
• /
• pp.1010-1018
• /
• 2008

In this report, we describe the use of gold nanoparticles (AuNPs) immobilized on pH. responsive, cross-linked poly(4-vinylpyridine) (P4VP) thin films, as a potential application for pH nanosensors. The methodology is based on the variation in surface plasmon resonance of immobilized AuNPs with changing the interparticle distances, caused by the swelling/deswelling of the pH responsive P4VP polymer films. The change in optical properties of the immobilized AuNPs in response to the pH of surrounding media was investigated by a simple yet powerful tool; UV-vis absorption spectroscopy. The swelling of the P4VP chains at pH 2 causes an increase in the interparticle distances of immobilized AUNPS ($\sim20nm$) and hence leads to a blue shift of 48 nm in their surface plasmon resonance band peak. On the other hand, when the surrounding media was altered from pH 2 to 10, a red shift of absorption maxima was observed. The changes were rapid, and the effect was reversible. This system could prove to be useful in fabricating nanosensors for detecting the pH or pH changes of surrounding aqueous medium.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2004.05a
• /
• pp.65-67
• /
• 2004

O-GlcNAcylation of p53 has been already identified and reported, but the function of O-GlcNAc on p53 has not been studied well. In this report, the general function of O-GlcNAc modification on p53 has been investigated using mouse fibroblast cell, L929. When streptozotocin (STZ), a non-competitive O-GlcNAcase inhibitor was treated to L929, O-GlcNAc modification level was dramatically increased on nucleocytoplasmic proteins, including p53. Because it has been already reported that $TNF\alpha$ induced the production of p53 in L929, $TNF\alpha$ was treated to obtain more p53. Approximately two times more amount of p53 was found from the cells treated STZ and $TNF\alpha$ simultaneously compared to the cell treated $TNF\alpha$ alone. The p53 increment in the presence of STZ was not caused by the induction of p53 gene expression. When new production of p53 induced by the $TNF\alpha$ was inhibited by the treatment of cycloheximide, O-GlcNAc modification decreased and phosphorylation increased on pre-existing p53 after $TNF\alpha$ treatment. But in the presence of STZ and $TNF\alpha$ at the same time, more O-GlcNAcylation occurred on p53, The level of ubiquitination on p53 was also reduced in the presence of STZ. Approximately three times less amount of Mdm2 bound to this hyperglycosylated p53. From this result it might be concluded that treatment of STZ to inhibit O-GlcNAcase increased O-GlcNAc modification level on p53 and the increment of O-GlcNAc modification stabilized p53 from ubiquitin proteolysis system.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.13 no.1
• /
• pp.1-9
• /
• 2011

This study was conducted to investigate the influence of different polyethylene film (P.E. film) for mulching on the changes in soil temperature and the production of good feathered apple nursery trees. M.9 rootstocks with stem diameter of 9.1~11.0 mm were planted in plots covered with different P.E. film (i.e., transparent P.E. film, black P.E. film, and bare soil as control). Three weeks after planting, the rootstocks were veneer grafted with 'Sinano Sweet' apple cultivar. In the middle of June, BA was sprayed to nursery for inducing feathering during the growing season. The soil temperature of the control was higher than air temperature by about $0.7^{\circ}C$ from mid April to early October, and that of P.E. film mulching was about $1{\sim}5^{\circ}C$ higher than that of the control. The soil temperature under transparent P.E. film was about $2{\sim}3^{\circ}C$ higher than that under black P.E. film. The diurnal range of soil temperature under the black P. E. film was lowest among all treatments. The P.E. film mulching induced better tree growth and feathering than bare soil. Percentage of good feathering apple nursery of black P.E. film was highest among all treatments because the soil temperature unuder black P.E. film in the early growing season was higher than that of the control and the number of days when the maximum soil temperature was over $35^{\circ}C$ in the summer was lower than that under the transparent P.E. film.

• Journal of Korean Academy of Fundamentals of Nursing
• /
• v.8 no.1
• /
• pp.51-68
• /
• 2001

The purpose of this study was to explain the relationship between smoking status, smoking behavior and hardiness in university students in Gangnung City. The subjects were a convenience sample of 315 students. The data were collected by a questionnaire given to the students between May 22 to June 2, 2000. An instrument developed by Akers & Gang(1996) and translated by Sohn, Jung-Nam(1999) was used in this study to measure definition of smoking, differential reinforcement of smoking and smoking behavior. The differential peer association scale developed Krohn et al.(1982) and translated by Sohn, Jung-Nam(1999), and the hardiness scale developed by Pollock(1984) and translated by Suh, Mun-Sa(1988) were also used. The data were analyzed using the SAS/PC+ Program and included descriptive statistics, t-test, ANOVA, and Spearman correlation coefficients. The results of this study are as follows : 1. The smoking rate for university students was 50.5% of which 44.7% started smoking in high school. 2. The mean score for level of hardiness was $3.14{\pm}0.43$ 3. The mean score for smoking behavior was as follows : 1) The mean score for the neutralizing definition was $2.16{\pm}0.57$ 2) The mean score for the negative definition was $2.37{\pm}0.71$ 3) The mean score for the positive differential reinforcement was $1.89{\pm}0.63$ 4) The mean score for the negative differential reinforcement was $2.96{\pm}0.64$ 5) The mean score for the differential peer association was $2.67{\pm}1.05$ 4. The data showed positive correlations between hardiness and the neutralizing definition(r=.1951, P<.001), between hardiness and the positive differential reinforcement(r=.1128, P<.05), between hardiness and the amount of smoking per day(r=.1452, P<.05) between the neutralizing definition and positive differential reinforcement(r=.4212, P<.0001), between the neutralizing definition and differential peer association(r=.1856, P<.001), between the neutralizing definition and age at smoking initiation(r=.1582, P<.05), between the negative definition and negative differential reinforcement(r=.2985, P<.0001), between the positive differential reinforcement and differential peer association(r=.3451, P<.0001), between positive differential reinforcement and the amount of smoking per day(r=.4431, P<.0001), between differential peer association and the duration of smoking(r=.2789, P<.0001), between differential peer association and the amount of smoking per day(r=.5410, P<.0001), between the duration of smoking and the amount of smoking per day(r=.4245, P<.0001). The data showed negative correlations between the neutralizing definition and the negative definition(r=-.2065, P<.001) between the neutralizing definition and negative differential reinforcement(r=-.3943, P<.0001) between the neutralizing definition and duration of smoking(r=-.1957, P<.05), between the negative definition and positive differential reinforcement(r=-.2093, P<.001), between the negative definition and the amount of smoking per day(r=-.2282, P<.001), between positive differential reinforcement and negative differential reinforcement(r=-.5555, P<.0001) between negative differential reinforcement and differential peer association(r=-.3653, P<.0001), between negative differential reinforcement and the amount of smoking per day(r=-.4570, P<.0001), between the age at smoking initiation and the duration of smoking(r=-.4594, P<.0001).

• Bulletin of the Korean Chemical Society
• /
• v.7 no.2
• /
• pp.137-142
• /
• 1986

The luminescence spectra of $Ru(bpy)_3^{2+}$ in poly(methacrylic acid) (PMA) solutions varied sensitively with pH. At pH < 5.5, the emission intensity increased with pH up to 4 times, while it decreased with pH beyond the pH. The enhanced emission intensity was accompanied by blue-shift of the emission maxima as much as 15 nm. The enhancement of emission intensity was attributed to the restricted rotational mobility of ligand of the cation bound to densely coiled PMA molecules at pH < 5.5. The sharp decrease in emission intensity with increasing pH near pH 5.6 was accounted for conformational transition of the polymer to more extended structure, which was also revealed in viscosity measurement. The enhancement of emission intensity became higher as NaCl concentration of the solution increased. The binding constant of $Ru(bpy)_3^{2+}$ with two carboxylate groups of PMA was calculated as $2{\times}10^5\;M^{-1}$ in 0.1 M NaCl at pH 5.2. The pH dependence of luminescence quenching rate of $Ru(bpy)_3^{2+}$ by $Cu^{++}$ also showed maximum near pH 5, and the rate was more than $10^3$ times higher than that in water, whereas the maximum enhancement of quenching rate (about 20 times) in poly(acrylic acid) (PAA) solution occurred at pH 4.5. On the other hand, the pH dependence for neutral water soluble nitrobenzene (NB) exhibited opposite trend to that of $Cu^{++}$. The quenching constant vs pH curve for $MV^{++}$ was composite of those for $Cu^{++}$ and NB. The anomalous high quenching rate for $Cu^{++}$ in PMA solution at pH < 5.5 was attributed to the binding of $Ru(bpy)_3^{2+}$ and $Cu^{++}$ to the same region of PMA, when it conforms densely coiled structure in the pH range. The observation of mininium quenching rate for NB near pH 5.5 indicated that the $Ru(bpy)_3^{2+}$ bound to the densely coiled PMA is not accessible by NB, which is in bulk water phase. The composite nature of the pH dependence of quenching rate for $MV^{++}$ in PMA solution was attributed to the smaller binding affinity of the cation to PMA, compared to that of $Cu^{++}$. The sharp, cooperative conformational transition with pH observed in PMA was not revealed in PAA. But, the pH dependence of quenching rates in this polymer reflected increased charge density and, thus, binding of cations to the polymer, and expansion of the polymer chain with pH.

• Journal of the Korean Society of Food Culture
• /
• v.28 no.4
• /
• pp.386-391
• /
• 2013

This study investigated the effects of freeze-dried sweet pumpkin powder on the baking of bread. Freeze-dried sweet pumpkin powder (0, 3, 6, 9, or 12%) was supplemented during the baking process and the bread volume, weight, specific volume, baking loss rate, texture, and sensory characteristics were measured. The results were following. Freeze-dried sweet pumpkin contained 4.88% water, 4.40% protein, and 4.02% ash. The L-values (brightness) of the bread were significantly lower as increasing amounts of sweet pumpkin powder were added (p<0.001). However, higher values for the a-value (redness) and b-value (yellowness) were observed (p<0.01 for both values). The volume and baking loss rate decreased when the sweet pumpkin powder was added. In contrast, the weight significantly increased as sweet pumpkin powder was added (p<0.01) and the intensity of dark brown color increased. The outer side of the bread was balanced overall, but the outer skin of the bread appeared darker as the amount of sweet pumpkin powder increased. The overall acceptability, hardness (p<0.001), elasticity (p<0.01), cohesiveness (p<0.01), gumminess (p<0.01), and brittleness (p<0.001) increased as sweet pumpkin powder increased as well. However, when sweet pumpkin powder was added at 12%, the overall acceptability value was fairly low. Sensory parameters, such as taste (p<0.001), color (p<0.001), and flavor (p<0.05) were observed when sweet pumpkin powder was used at 3% and these results were lower than the control. Overall acceptability (p<0.001) was best when sweet pumpkin powder was used at 9% and the texture was best when sweet pumpkin powder was used at 6%.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.31 no.1
• /
• pp.101-107
• /
• 2003

Focusing on native plants that have a high possibility of being introduced as extensive rooftop material, this study was conducted to realize extensive and easy-to-manage rooftop gardens and to raise the utilization of native plants by verifying their growing response to soil media and depth. Its result is as follows: 1) In the case of Chrysanthemum zawadskii, the top growth was better in sandy loam than in P$_1$V$_1$P$_2$, and P$_1$V$_1$P$_3$, but the mortality rate was high, making it unsuitable soil. Regarding soil depth the mortality rate was lower in 10cm than in 5cm, and it grew well in 10cm. When using it for rooftop gardens, it would be desirable to keep the minimum viable soil depth over loom. 2) In the case of Sedium middendorffianum the mortality rate was 0% regardless of soil media and depth making it very suitable material for rooftop garden. Although the flowering rate was somewhat lower in P$_1$V$_1$P$_2$, and P$_1$V$_1$P$_3$ than in sandy loam, the mortality rate was low and the root growth was good. Therefore, provided that fertilizing is managed well, it is a plant that can be highly utilized. 3) In the case of Allium senescens, the mortality rate was 0% regardless of soil or soil depth, making it a very suitable plant for extensive rooftop gardens. Although top growth was poorer in P$_1$V$_1$P$_2$, and P$_1$V$_1$P$_3$than in sandy loam the root growth was good. Therefore, provided that fertilizing is managed well, it is a plant that can be highly utilized. In conclusion the study revealed that suitable species for extensive rooftop gardens are Sedium middendorffianum and Allium senescens. However, Chrysanthemum zawadskii can be utilized greatly when fertilizing is managed regularly in artificial mixed soil over l0cm.

• Journal of Oral Medicine and Pain
• /
• v.40 no.3
• /
• pp.124-129
• /
• 2015

Purpose: Salivary pH is an easily measurable biochemical marker and related to various intraoral and systemic conditions. The aim of this study was to evaluate the reliability of the salivary pH measurement using pH paper. In addition, the normal values of salivary pH using pH paper were compared to those of pH meter to investigate the validity. Methods: Twenty healthy male participants attended this study (mean age, $24.5{\pm}1.47$ years). Unstimulated saliva and stimulated saliva were collected from each subject two times with the interval of a day and salivary pH was immediately measured by the two experienced examiners using pH paper and pH meter. The salivary pH was compared between the groups and inter- and intra-examiner reliability of pH paper was investigated. The intraclass correlation coefficient (ICC) was used to calculate variations. Results: All measurements had good to excellent inter-examiner (ICC 0.755 for unstimulated; 0.760 for stimulated saliva), intra-examiner (ICC 0.635 for unstimulated; 0.592 for stimulated saliva) reliability and two measurement methods using pH paper and pH paper also showed high reliability (ICC 0.852 for unstimulated; 0.640 for stimulated saliva). The values measured by pH paper were significantly lower than those measured by pH meter. Conclusions: pH paper showed adequate inter- and intra-examiner reliability and it presented the validity in terms of comparison with the pH meter as a standard in the salivary pH measurement.