• 제목/요약/키워드: WATER STRESS

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Isolation an Identification of the Active Compounds from Green Mustard Leaves against Oxidative Stress in Bovine Brain Tissue (청겨자채 추출물의 뇌조직 내 Oxidative Stree 억제활성물질의 동정)

  • 이근종;김미리;심재석;황재관
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.943-948
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    • 2001
  • Green mustard leves were found to effectively prevent lipid peroxidation of bovine-brain tissue by ascor-bate/Fe system, The 50% methanol extracts mustard leaves were separated into four solvent faction using n-hexane,. EtOAc, n-BiOH and water. Then n-BiOH fraction exclusively exhibited the antioxidative activities at concentration above 100 $\mu\textrm{g}$/mL/ The n-BuOH fraction was further isolated to a single compound using TLC analysis and silica gel chromatography. The active antiodidative compounds were identified as sinapic acid methyl ester and ferulic acid methyl ester by $^{1}$H-NMR and $^{13}$ C-NMR, The sinapic acid methyl ester and ferulic acid methyl ester were prepared by methylating of sinapic acid and ferulic acid with diazomethane. The results strongly suggested that sinapic acid and ferulic acid could be emplyed as a potential antioxiative agents for preventing the bovine brain lipid peroxidation. lipid peroxidation.

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Development of Numerical Method for Large Deformation of Soil Using Particle Method (입자법을 이용한 토사의 대변형 해석법 개발)

  • Park, Sung-Sik;Lee, Do-Hyun;Kwon, Min-Ho
    • Journal of the Korean Geotechnical Society
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    • v.29 no.12
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    • pp.35-44
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    • 2013
  • In this study, a particle method without using grid was applied for analysing large deformation problems in soil flows instead of using ordinary finite element or finite difference methods. In the particle method, a continuum equation was discretized by various particle interaction models corresponding to differential operators such as gradient, divergence, and Laplacian. Soil behavior changes from solid to liquid state with increasing water content or external load. The Mohr-Coulomb failure criterion was incorporated into the particle method to analyze such three-dimensional soil behavior. The yielding and hardening behavior of soil before failure was analyzed by treating soil as a viscous liquid. First of all, a sand column test without confining pressure and strength was carried out and then a self-standing clay column test with cohesion was carried out. Large deformation from such column tests due to soil yielding or failure was used for verifying the developed particle method. The developed particle method was able to simulate the three-dimensional plastic deformation of soils due to yielding before failure and calculate the variation of normal and shear stresses both in sand and clay columns.

Ginsenoside Contents and Antioxidative Activities from Red Ginseng Treated with High Hydrostatic Pressure (초고압 처리한 홍삼의 ginsenoside 함량 및 항산화 활성)

  • Shin, Chang-Sik;Lee, Do-Hyun;Kim, Sung-Han;Shin, Min-Ho;Jeong, Chang-Ho;Shim, Ki-Hwan
    • Journal of agriculture & life science
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    • v.44 no.6
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    • pp.133-140
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    • 2010
  • This study was carried out to investigate the changes of ginsenoside contents and antioxidative activities from red ginseng after treated with high hydrostatic pressure (RGHHP). Crude saponin content in traditional red ginseng (TRG) and RGHHP were 21.93 and 27.29 mg/g, respectively. The contents of total phenolics, crude saponin and ginsenoside increased after treated with high hydrostatic pressure. TRG and RGHHP showed an increase 25.60% the highest content of Rb1 (14.10 mg/g and 17.71 mg/g). Also, Rg3 content compared with TRG and RGHHP increased 10.46%. The radical scavenging activity of hot water extract from red ginseng against the DPPH and ABTS radicals increased with the increasing amount of extract and RGHHP higher than TRG. The reducing power and ferric reducing antioxidant power (FRAP) assays of the red ginseng were increased in a dose dependent manner. The FRAP of TRG and RGHHP were 0.30 and 0.36 absorbance, respectively at a concentration of 10 mg/mL. The present results suggest that RGHHP would have the protective potential from oxidative stress induced by free radicals.

A Study on Surface Properties of Mechanical Interfacial Behavior of DGEBA/PMR-15 Blends (DGEBA/PMR-15 블렌드계의 표면특성 변화가 기계적 계면특성에 미지는 영향)

  • Park, Soo-Jin;Lee, Hwa-Young;Han, Mijeong;Hong, Sung-Kwon
    • Journal of Adhesion and Interface
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    • v.4 no.1
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    • pp.1-8
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    • 2003
  • In this work, the effect of PMR-15 content on the variation of surface free energy of the DGEBA/PMR-15 blend system was investigated in terms of contact angles and mechanical interfacial tests. Based on FT-IR result of the blend system. C=O (1,772, $1,778cm^{-1}$) and C-N ($1,372cm^{-1}$) peaks appeared with imidization of PMR-15 and -OH ($3,500cm^{-1}$) peak showed broadly at 10 phr of PMR-15 by ring-opening of epoxy. Contact angle measurements were performed by using deionized water and diiodomethane as testing liquids. As a result, the surface free energy of the blends gave a maximum value at 10 phr of PMR-15, due to the significant increasing of specific component. The mechanical interfacial properties measured from the critical stress intensity factor ($K_{IC}$) and the critical strain energy release rate ($G_{IC}$) showed a similar behavior with the results of surface energetics. This behavior was probably attributed to The improving of the interfacial adhesion between intermolecules, resulting from increasing the hydrogen bondings of the blends.

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A Study of Skin Biophysical Parameters and Biomarkers related to the Anatomical Site and Age in Korean Women (한국 여성의 피부 부위 및 연령에 따른 피부 측정 인자와 생물 인자 연구)

  • Cho, Seok-Cheol;Nam, Gaewon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.4
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    • pp.413-420
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    • 2015
  • The skin is one of the largest organs in our body and participates in many of the human organism's physiological and pathological events. Skin function were known for self-maintenance and self-repair, mechanical and chemical stress protection, protection against UV and environmental pathogenic micro-organisms, production of vitamin D, and social and psychological function through the physical aspect. The aim of this study was to evaluate the variation of biophysical parameters and to find relation with skin biomarkers in different anatomical site and age in Korean women. About 70 healthy volunteers in age range 20 to 49 were participated in this test. Test areas were the forearms and the cheek. Investigation to determine biophysical parameters on human skin, was carried out using various non-invasive methods. For analysis to skin biomarkers, we studied to examine various biomarkers for the quantitative determination of cortisol, fibronectin, keratin-1, 10, and 11, involucrin, and keratin-6 in human face and forearm. And we measured to skin biophysical parameters for skin anatomical site and age difference with non-invasive methods. As results of measuring site, some parameters were have following significant difference, stratum corneum hydration, trans epidermal water loss and skin color (L and a value). As results of age difference, skin colors were had only significant difference with age. For cortisol, keratin-6, fibronectin, keratin-1, 10, 11 contents, there were no significant difference in age and site. However, involucrin level in the cheeks were the highest for age group 30 ~ 39 compared to other age groups. These results suggest that in individual skin condition may explain detailed skin state variation.

Antimicrobial activity and protective effect of Geranium thunbergii against oxidative DNA damage via antioxidant effect (현초의 항산화 활성에 의한 산화적 DNA 손상 보호효과 및 항균활성)

  • Kwon, Tae-Hyung;Lee, Su-Jin;Park, Jae-Ho;Kim, Taewan;Park, Jung-Ja;Park, Nyun-Ho
    • Food Science and Preservation
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    • v.24 no.3
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    • pp.325-333
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    • 2017
  • This study aimed to investigate the various biological activities of Geranium thunbergii such as antimicrobial activity and protective effect against oxidative damage. To evaluate its antioxidant and antimicrobial activities, we first performed methanol extraction; this methanol extract was further partitioned using various solvents. And then, its antioxidant activity was measured using various assays including total phenolic content and protection against oxidative DNA damage, and antimicrobial activities were examined using minimum inhibiting concentration (MIC) test, and paper disc method. In addition, high-performance liquid chromatography was performed to analyze the major chemical components of ethyl acetate fraction. The G. thunbergii fraction with ethyl acetate exhibited higher antioxidant and antimicrobial activities than the other fractions. The results showed that G. thunbergii ethyl acetate fraction at $50{\mu}g/mL$ had strong DPPH and ABTS radical scavenging activities of 80.88% and 80.12%, respectively. In addition, the ethyl acetate fraction protected DNA from the oxidative damage induced by ferrous ion and hydroxyl radicals and showed high antimicrobial activity with diameter of inhibition zones ranging from 13.33 to 15.67 mm. High-performance liquid chromatography analysis revealed the major phenolic compounds of G. thunbergii to be ellagic acid and gallic acid. These results suggest that G. thunbergii might protect DNA against oxidative stress induced by reactive oxygen species and can be utilized as a natural source of antioxidant and antimicrobial agent in the food industry.

The Study of Efficient Estimation of GPS Photogrammetry (GPS 항공사진측량의 효율성 평가에 관한 연구)

  • Kim, Young-Suk;Shon, Ho-Woong
    • Journal of the Korean Geophysical Society
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    • v.9 no.2
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    • pp.121-128
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    • 2006
  • Recently, spillways are need to control stable water level for supporting main dams because of floods by unusual change of weather such as Typhoon Rusa. This study has been focused on the amount of leakage through the rock mass distributed fractures and joints under the opened emergency spillway. It is very important to evaluate the amount of leakage as these affect stability of spillway by interaction between effective stress and pore pressure. The commercial program MAFIC has been used for analyzing groundwater flow in fractured rock mass. The results showed that the values of range, average and deviation of leakage were 2.85∼ 3.79×10-1, 3.32×10-1 and 1.70×10-2 m3/day/m2 respectively. Secondary, we have estimated the effect of grouting after the transmissivity(Tf) of joint 1 as main pathway of leakage known from above results was changed from 1.78×10-7 to 1.59×10-9 m2/s. The results showed that the values of range, average and deviation of leakage were 7.80×10-4∼1.53×10-3, 1.18×10-3 and 1.32×10-4 m3/day/m2 respectively. As the result, the amount of leakage after grouting has been decreased by a ratio of 1 to 277.

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Drone Image based Time Series Analysis for the Range of Eradication of Clover in Lawn (드론 영상기반 잔디밭 내 클로버의 퇴치 범위에 대한 시계열 분석)

  • Lee, Yong Chang;Kang, Joon Oh;Oh, Seong Jong
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.39 no.4
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    • pp.211-221
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    • 2021
  • The Rabbit grass(Trifolium Repens, call it 'Clover') is a representative harmful plant of lawn, and it starts growing earlier than lawn, forming a water pipe on top of the lawn and hindering the photosynthesis and growth of the lawn. As a result, in competition between lawn and clover, clover territory spreads, but lawn is damaged and dried up. Damage to the affected lawn area will accelerate during the rainy season as well as during the plant's rear stage, spreading the area where soil is exposed. Therefore, the restoration of damaged lawn is causing psychological stress and a lot of economic burden. The purpose of this study is to distinguish clover which is a representative harmful plant on lawn, to identify the distribution of damaged areas due to the spread of clover, and to review of changes in vegetation before and after the eradication of clover. For this purpose, a time series analysis of three vegetation indices calculated based on images of convergence Drone with RGB(Red Green Blue) and BG-NIR(Near Infra Red)sensors was reviewed to identify the separation between lawn and clover for selective eradication, and the distribution of damaged lawn for recovery plan. In particular, examined timeseries changes in the ecology of clover before and after the weed-whacking by manual and brush cutter. And also, the method of distinguishing lawn from clover was explored during the mid-year period of growth of the two plants. This study shows that the time series analysis of the MGRVI(Modified Green-Red Vegetation Index), NDVI(Normalized Difference Vegetation Index), and MSAVI(Modified Soil Adjusted Vegetation Index) indices of drone-based RGB and BG-NIR images according to the growth characteristics between lawn and clover can confirm the availability of change trends after lawn damage and clover eradication.

Antioxidant, Antibacterial and Anti-inflammatory Effects of Stachys sieboldii Extract (초석잠 추출물의 항산화, 항균 및 항염 활성)

  • Lee, Jeong Ho
    • Korean Journal of Plant Resources
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    • v.34 no.5
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    • pp.420-432
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    • 2021
  • This study was conducted to evaluate antioxidant, antibacterial, anti-inflammatory, and digestive enzyme activity in water extract (SAW) and 60% ethanol extract (SAE) from Stachys sieboldii. As the treatment concentration of each extract S. sieboldii extract increased, antibacterial and antioxidant activity increased. The total polyphenol and total flavonoid contents of SAW were 106.25 ± 0.94 mgGAE/g, 24.4 ± 0.24 mgQE/g and SAE were 124.61 ± 1.11 mgGAE/g, 45.2 ± 3.52 mgQE/g, respectively. The 400 ㎍/mL of SAW and SAE performed more than 53% protective effects against oxidative stress in HepG2 cell lines. All extracts were not showed cytotoxicity to RAW 264.7 cell line at 100 ㎍/mL. NO production was reduced to 44.3 ± 1.4% for SAW and 45.1 ± 1.0% for SAE at a concentration of 100 ㎍/mL. The production of inflammatory cytokines each TNF-α, IL-1β, and IL-6 was inhibited in a concentration-dependent. S. sieboldii extract did not showed Caco-2 cells cytotoxicity and inhibited NO production in concentration-dependent. As the concentration of the S. sieboldii extract increased of α-amylase and protease enzymes activity, which are digestive enzyme. As a result of the experiment, it is judged that it can be used as basic data for the development of health food using S. sieboldii.

A Study on Correlation between Species Composition and Environmental Factors in Abies koreana Forest (구상나무림의 종조성과 환경인자간 상관관계)

  • Park, Byeong Joo;Kim, Ji Dong;Lee, Jun Woo;Cho, Seung wan;Heo, Tae Im;Lee, Dong Hyuk;Byeon, Jun Gi
    • Korean Journal of Plant Resources
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    • v.34 no.2
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    • pp.144-155
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    • 2021
  • This study was conducted to analyze community structure and species composition of Abies koreana forest. Field survey was carried out from June, 2019 to 2020, September. 121 plots (20×20 m) were set up, 5 herb layer plots (3×3 m) were constructed for each plot, and there, Diameter at Breast Height(DBH), height, environmental factor were measured. It was analyzed that water stress did not have a significant effect on the factors affecting the growth of A. koreana in Mt. Halla-san, and in the case of Mt. Deokyu-san, the more forest stands located at the ridge, the higher the tree density. It was found that the factors affecting the distribution of Abies koreana were not consistent according to the environment of study site.