• Title/Summary/Keyword: WATER STRESS

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A new culture system for in situ observation of the growth and development of Eucyclops serrulatus (Copepoda: Cyclopoida)

  • PARK Sung-Hee;CHANG Cheon-Young;SHIN Sung-Shik
    • Parasites, Hosts and Diseases
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    • v.43 no.4 s.136
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    • pp.141-147
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    • 2005
  • A practical and convenient method of rearing Eucyclops serrulatus in a microculture environment is described. A complete life cycle of E. serrulatus was maintained in a narrow space on a microscope slide glass on which a cover glass of $22{\times}40mm$ in size was mounted at a height of 0.8mm. The culture medium was constituted by bottled mineral water boiled with grains of Glycine max (soybean). Chilomonas paramecium, a free-living protozoan organism, was provided as live food. Growth of nauplii hatched from eggs to the first stage of copepodite took an average of 7.7 days, and the growth of copepodite 1 to the egg-bearing adult female took an average of 20.1 days in the microculture cell with an average life time of 44.7 days. Continuous passage of cope pods was successfully maintained as long as sufficient medium and food were provided. The microculture method enables an in situ microscopic observation on the growth and developmental process of helminth larvae experimentally infected to copepods as well as of copepod itself. Furthermore, it does not require anesthetization and, therefore, minimize the amount of stress exposed to cope pods during the handling process.

Cryopreservation and low-temperature storage of seeds of Phaius tankervilleae

  • Hirano, Tomonari;Godo, Toshinari;Miyoshi, Kazumitsu;Ishikawa, Keiko;Ishikawa, Masaya;Mii, Masahiro
    • Plant Biotechnology Reports
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    • v.3 no.1
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    • pp.103-109
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    • 2009
  • In this study we established reliable methods for conservation of seeds of Phaius tankervilleae as an orchid genetic resource. The seeds, which were dehydrated to 5% water content and preserved at $4^{\circ}C$, showed no decrease in viability and germinability after three months. After storage for six months, however, the seeds showed a drastic decrease in germinability, even though survival rate was high. For long-term preservation of seeds of P. tankervilleae, cryopreservation is applied to the freshly harvested seeds. When the seeds were cryopreserved by the vitrification method for up to 12 months there was no apparent deterioration effect of storage time. These results indicate that cryopreservation by the vitrification method is useful for long-term conservation of P. tankervilleae seeds, which are difficult to preserve for more than three months under dry and low-temperature conditions.

Fatigue Life Evaluation and Optimization for District Heating Pipes Considering Operating Temperature Transition Data (운전이력을 고려한 지역난방 열배관의 피로수명 평가 및 최적화)

  • Ahn M.Y.;Jung S.W.;Lee S.M.;Chang Y.S.;Choi J.B.;Kim Y.J.;Kim S.H.;Kim Y.H.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.581-584
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    • 2005
  • A district heating(DH) system supplies environmentally-friend heat and is appropriate for reduction of energy consumption and/or air pollutions. The objectives of this paper are to systematize data processing of transition temperature, investigate its effects on fatigue life of DH pipes and optimization for size of DH pipes. A relational database management system as well as reliable fatigue life evaluation procedures is established for Korean DH pipes. Also, since the prototypal evaluation results satisfied both cycle-based and stress-based fatigue criteria. Through the optimum design process, the cross section diminished 18.64% and the CUF diminished 23.35%. So, it can be used as useful information in the future for optimal design, operation and energy saving via setting of efficient condition and stabilization of water temperature.

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Development of Construction Simulation Apparatus on Centrifugal Experiment (원심모형실험을 위한 시공단계모사장비개발)

  • Kim, You-Seok;Kim, Kyoung-O;Lee, Jong-Pil;Park, Jin-Woo
    • Proceedings of the Korean Geotechical Society Conference
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    • 2010.09a
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    • pp.979-990
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    • 2010
  • Although a centrifuge model test is performed with scaled models, it has a lot of advantages compared with usual scale model tests, for the reproduction of stress levels equal to a full scale test is possible. At the beginning of the Daewoo Institute of Construction Technology, a servo-motor-driven single axis actuator was introduced and has been in use with a geo-centrifuge. However, for variety of experiments and construction stage simulation, various apparatuses have been developed, such as a vacuum generator, a lateral actuator for tidal power simulation, a gravel hopper and a sand drainer for filled-up ground, and a water level controller. The apparatuses have been manufactured with enough strength and durability to be operated under specific g levels. This paper presents the properties of the apparatuses and the results of the tests performed with those.

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Study on the Breakdown Performance of Synthetic Polypropylene Laminated Paper for Application of a HTS Cable (고온초전도 케이블의 응용을 위한 PPLP의 절연파괴 특성연구)

  • Kwag, Dong-Soon;Kim, Young-Seok;Kim, Hae-Jong;Kim, Sang-Hyun
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.05e
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    • pp.65-68
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    • 2003
  • In this paper, we researched breakdown characteristics of paper/liquid nitrogen composite insulation system for application of a high temperature superconducting cable. And, we have studied the AC breakdown performance of paper/ice composite insulating system immersed in liquid nitrogen. The electric strength in this paper/ice composite system is higher than that of paper/liquid nitrogen system. Furthermore this system shows a self-healing type breakdown behavior. Among the many kinds of liquid to be immersed and frozen to the ice, deionized water gives the highest electric breakdown strength. The paper/ice composite insulation system is thought to be one of good candidate for the electrical insulating system at cryogenic temperature.

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Inhibitory Activity of the Fruit Extract of Gardenia jasminoides on Monoamine Oxidase (치자추출물의 Monoamine Oxidase 저해활성)

  • Park, Tae-Kyu;Hwang, Keum-Hee
    • Korean Journal of Pharmacognosy
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    • v.38 no.2 s.149
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    • pp.108-112
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    • 2007
  • We examined the inhibitory activities against monoamine oxidase (MAO) of Gardenia jasminoides in vitro and in vivo methods. Methanolic extract and ethylacetate fraction of Gardenia jasminoides fruit showed a significant inhibitory activity on MAO-A and MAO-B in vitro. The IC$_{50}$ values of each fraction on MAO-A and MAO-B are as fo11owed; total methanol extracts 1.23 and 1.34 mg/ml, EtOAc fraction 0.72 and 0.77 mg/ml. Water-soluble fraction also showed IC$_{50}$ values of 0.81 mg/ml on MAO-B. MAO-A activity was increased by the oral administration of ethanolic extract of G. jasminoides, while MAO-B activity was decreased. The concentration of serotonin of brain tissue administrated of ethanolic extract of G. jasminoides is slightly increased in rat. This tendency is not different from the activity of deprenyl which is a well known MAO inhibitor was used as a positive control. Consequently, we suggest that G. jasminoides may have the effects on the inhibitory activity against MAO This activity of G. jasminoides is considerable for development of functional materials for treatment and control of depression, dementia, Parkinson' disease, stress and promoting exercise, etc.

Mechanism of Consolidation Displacement on Internal Behavior of Clay Ground Improved by Sand Drain (샌드 드레인으로 개량된 점토지반의 내부거동에 대한 압밀변형 메커니즘)

  • Baek, Won-Jin
    • Journal of The Korean Society of Agricultural Engineers
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    • v.48 no.6
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    • pp.69-77
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    • 2006
  • In this study, the large scaled model test improved by sand drain was carried out to clarify the internal behavior of the three-dimensional consolidation under different secondary consolidation periods. From the results of model test, the void ratio in the undrained side was lager than in the drained side. In addition, the unconfined compressive strength in the long-term consolidated specimen was larger than that in the short-term consolidated one. It was also found that the unconfined compressive strength was larger in the drained side than in the undrained side. These reasons are considered to be due to the large effective stress by quick pore water pressure dissipation by the short drainage distance in the drained side. Furthermore, in order to investigate the three-dimensional consolidation behavior of clay ground improved by the vertical drain method, the numerical analysis obtained from the three-dimensional elasto-viscous consolidation theory proposed by author (2006) were compared with the test results. It was found that during the three-dimensional consolidation process not only vertical displacement but also radial displacement occurs inside the specimen.

Effect of Temperature and Aging on the Relationship between Dynamic and Static Elastic Modulus of Concrete (온도와 재령이 콘크리트의 동탄성계수와 정탄성계수의 상관관계에 미치는 영향)

  • 한상훈;김진근
    • Proceedings of the Korea Concrete Institute Conference
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    • 2001.11a
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    • pp.445-450
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    • 2001
  • The paper investigates the relationships between dynamic elastic modulus and static elastic modulus or compressive strength according to curing temperature, aging, and cement type. Based on this investigation, the new model equations are proposed. Impact echo method estimates the resonant frequency of specimens and uniaxial compression test measures the static elastic modulus and compressive strength. Type I and V cement concretes, which have the water-cement ratios of 0.40 and 0.50, are cured under the isothermal curing temperature of 10, 23, and 50 $^{\circ}C$. Cement type and aging have no large influence on the relationship between dynamic and static elastic modulus, but the ratio of dynamic and static elastic modulus comes close to 1 as temperature increases. Initial chord elastic modulus, which is calculated at lower strain level of stress-strain curve, has the similar value to dynamic elastic modulus. The relationship between dynamic elastic modulus and compressive strength has the same tendency as the relationship between dynamic and static elastic modulus. The proposed relationship equations between dynamic elastic modulus and static elastic modulus or compressive strength properly estimates the variation of relationships according to cement type, temperature, and aging.

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Development of float off Operation Design for Mdlti Semi-submersible Barges with Symmetrical Stability Casings (반 잠수식 복수부선의 진수설계)

  • 양영태;최문길;이춘보;박병남;성석부
    • Journal of Ocean Engineering and Technology
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    • v.17 no.2
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    • pp.72-76
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    • 2003
  • This paper presents the design concept and operation results of float-off for FSO (340,000 DWT Class, ELF AMENAM KPONO Project) built on the ground, without dry dock facilities. It was the first attempt to build FSO, completely, on the ground and launch it using DBU (Double Barge Unit, which was connected by rigid frame structure.) The major characteristics of FSO, which are similar to general VLCC type hull, including topside structure, weigh 51,000 metric ton. In order to have sufficient stability during the deck immersion of DBU, while passing through a minimum water plane area zone, proper trim control was completed with LMC (Load Master Computer). The major features of the monitoring system include calculation for transverse bending moment, shear force, local strength check of each connector, based on component stress, and deformation check during the load-out and float-off. Another major concern during the operation was to avoid damages at the bottom and sides of FSO, due to motion & movement after free-floating; therefore, adequate clearances between DBU and FSO were to be provided, and guide posts were installed to prevent side damage of the DBU casings. This paper also presents various measures that indecate the connector bending moment, damage stability analysis, and mooring of DBU during float off.

Analysis of Structural Characteristics of HDPE Pipe for Manganese Lifting Test (근해역 양광시험을 위한 HDPE Pipe의 구조특성 연구)

  • Lee, Jae-Hwan;Yoon, Chi-Ho
    • Journal of Ocean Engineering and Technology
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    • v.25 no.6
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    • pp.86-90
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    • 2011
  • The mining of imitated manganese noodles in 1000 m of seawater is planned for 2012. Thus, it is necessary to prepare the lifting pipes to be used for the test. Because of storage and expense constraints, flexible and economic HDPE pipe is being considered, making it necessary to test the structural safety. Material, pressure-chamber tests and finite element analysis of HDPE pipe for the 1000-m depth were performed. The tangential stiffness of HDPE was obtained through tension and three-point bending material tests and used for a structural analysis. FEA results show that the current sample pipe segment is safe for 1000 m of water pressure, and the stress result is also within the safe value. From the current results, the HDPE pipe seems to be acceptable only for the currently suggested constraints. However, more numerical and pressure tests need to be considered by applying additional physical conditions such as gravitational and hydrodynamic loads, external and internal fluid pressure, axial force induced ship motion, and heavy pump pressure to determine future usage.