• Journal of Plant Biotechnology
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• 제49권3호
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• pp.171-177
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• 2022

The precise homeostatic regulation of local auxin accumulation in xylem precursors of cambium stem cell tissues is one of the most important mechanisms for plant vascular patterning and radial secondary growth. Walls are thin (WAT1), a novel intracellular auxin transporter, contributes directly to the auxin accumulation maxima in xylem precursors. According to recent research, the auxin signaling activated pathway-related gene network was significantly enriched during the secondary growth of Panax ginseng storage roots. These imply that during P. ginseng root secondary growth, specific signaling mechanisms for local auxin maxima in the vascular cambial cells are probably triggered. This study identified four WAT1-like genes, PgWAT1-1/-2 and PgWAT2-1/-2, in the P. ginseng genome. Their expression levels were greatly increased in nitratetreated storage roots stimulated for secondary root growth. PgWAT1-1 and PgWAT2-1 were similar to WAT1 from Arabidopsis and tomato plants in terms of their subcellular localization at a tonoplast and predicted transmembrane topology. We discovered that overexpression of PgWAT1-1 and PgWAT2-1 was sufficient to compensate for the secondary growth defects observed in slwat1-copi loss of function tomato mutants. This critical information from the PgWAT1-1 and PgWAT2-1 genes can potentially be used in future P. ginseng genetic engineering and breeding for increased crop yield.

• 식품과학과 산업
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• 제50권1호
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• pp.26-35
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• 2017

Obesity is a worldwide problem that is associated with metabolic disorders. Obesity is caused by the accumulation of an abnormal amount of body fat in adipose tissue. Adipose tissue is a major metabolic organ, and it has been classified as either white adipose tissue (WAT) or brown adipose tissue (BAT). WAT and BAT are characterized by different anatomical locations, morphological structures, functions, and gene expression patterns. WAT is mainly involved in the storage and mobilization of energy in the form of triglycerides. On the other hand, BAT specializes in dissipating energy as heat through uncoupling protein-1 (UCP-1)-mediated non-shivering thermogenesis. Novel type of brown-like adipocyte within WAT called beige/brite cells was recently discovered, and this transdifferentiation process is referred to as the "browning" or "britening" of WAT. Recently, Brown fat and/or browning of WAT have been highlights as a new therapeutic target for treatment of obesity and its related metabolic disorders. Here, we describe recent advances in the study of BAT and browning of WAT, focusing on proteomic approaches.

• 한국토양비료학회지
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• 제29권3호
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• pp.297-302
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• 1996

돈분 및 계분을 시비수준을 달리하여 표토(16cm)에 처리한후 1주일 후부터 토마토를 재배하면서 근권토양내의 미생물상의 변화를 조사한 결과는 다음과 같다. 1. 무처리구의 근권총세균 밀도는 시비구 보다 적었으며, 생육기인 5주째에 균밀도가 1주째보다 떨어졌으며 15주째에는 최고가 되었다. 희석영양배지(Diluted Nutrient Broth : NB)의 세균밀도는 영양배지(Nutrient Broth NB)에서 보다 1.5~2배 높게 나타났다. 2. 정식 1주일후의 근권 토양 세균 수는 돈분에서 NB와 DNB배지에서 퇴비량에 비례하여 증가하였으며, 계분에서는 NB는 증가하였으나 DNB는 시용량에 따라 무비구 보다 감소했다. 60, 120 Mg/ha처리구에서 무비구에 비해 NB에서 100배 이상 증가하였다. 15주후인 토마토 수확기에는 1주후의 경우와 반대로 DNB배지의 세균수가 NB배지에서 보다 높았다. 3. 사상균수는 유기질 비료 시용 1주후에 시비수준에 따라서는 큰 차이를 나타내지 않았지만, 계분시용구에서 무시구보다 감소하였다. 15주째에 계분은 2주째보다 2배가 높았으며 계분 30 Mg/ha에서 최고로 높았으나 돈분은 시용량에 따라 무비구보다 감소하였다. 4. 방선균의 밀도는 1주후 돈분구에서 차이가 없고 계분 시용구에서 무비구보다 감소하였고 15주째에 모든시비구에서 크게 증식하여 돈분 10 Mg/ha와 계분 30 Mg/ha구의 경우 1주후 토양에 비하여 4배 이상 증가하였다.

• Journal of Ginseng Research
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• 제40권2호
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• pp.141-150
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• 2016

Background: Adipocyte-macrophage communication plays a critical role regulating white adipose tissue (WAT) inflammatory gene expression. Because WAT inflammation contributes to the development of metabolic diseases, there is significant interest in understanding how exogenous compounds regulate the adipocyte-macrophage crosstalk. An aqueous (AQ) extract of North American (NA) ginseng (Panax quinquefolius) was previously shown to have strong inflammo-regulatory properties in adipocytes. This study examined whether different ginseng extracts influence adipocyte-macrophage crosstalk, as well as WAT inflammatory gene expression. Methods: The effects of AQ and ethanol (EtOH) ginseng extracts ($5{\mu}g/mL$) on adipocyte and macrophage inflammatory gene expression were studied in 3T3-L1 and RAW264.7 cells, respectively, using real-time reverse transcription polymerase chain reaction. Adipose tissue organ culture was also used to examine the effects of ginseng extracts on epididymal WAT (EWAT) and inguinal subcutaneous WAT (SWAT) inflammatory gene expression. Results: The AQ extract caused significant increases in the expression of common inflammatory genes (e.g., Mcp1, Ccl5, Tnf-${\alpha}$, Nos2) in both cell types. Culturing adipocytes in media from macrophages treated with the AQ extract, and vice versa, also induced inflammatory gene expression. Adipocyte Ppar-${\gamma}$ expression was reduced with the AQ extract. The AQ extract strongly induced inflammatory gene expression in EWAT, but not in SWAT. The EtOH extract had no effect on inflammatory gene expression in either both cell types or WAT. Conclusion: These findings provide important new insights into the inflammo-regulatory role of NA ginseng in WAT.

• Molecules and Cells
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• 제47권2호
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• pp.100030.1-100030.14
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• 2024

Both brown and white adipose tissues (BAT/WAT) are innervated by the peripheral nervous system, including efferent sympathetic nerves that communicate from the brain/central nervous system out to the tissue, and afferent sensory nerves that communicate from the tissue back to the brain and locally release neuropeptides to the tissue upon stimulation. This bidirectional neural communication is important for energy balance and metabolic control, as well as maintaining adipose tissue health through processes like browning (development of metabolically healthy brown adipocytes in WAT), thermogenesis, lipolysis, and adipogenesis. Decades of sensory nerve denervation studies have demonstrated the particular importance of adipose sensory nerves for brown adipose tissue and WAT functions, but far less is known about the tissue's sensory innervation compared to the better-studied sympathetic nerves and their neurotransmitter norepinephrine. In this review, we cover what is known and not yet known about sensory nerve activities in adipose, focusing on their effector neuropeptide actions in the tissue.

• 농업과학연구
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• 제41권4호
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• pp.291-298
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• 2014

Plastic film houses are directly associated with increases in plant growth and yield of vegetable crops through a year round cultivation, however, at the same time temperature stresses are one of fates which are difficult to avoid during crop growth. The objective of this study was to examine the translocation and distribution of minerals (N, P, K) and carbohydrates as well as seasonal fluctuation of mineral uptake and carbohydrate production in cucumber plant grown under moderately high temperature. The temperature treatments consisted of 2-layers film houses (optimal temp.) and 3-layers (high temp.). Shoot growth of cucumber plants were linearly increased until 14 weeks after transplanting (WAT) without any significant difference between both temperatures, and the slowdown was observed from 16 WAT. The level of soluble sugar and starch was slightly greater in optimal temperature compared to the high. Cumulative accumulation of soluble sugar was significantly different before and after 12 WAT in both treatments, whereas starch level represented a constant increase. Monthly production of soluble sugar reached the peak between 12 to 16 WAT, and starch peaked between 4 to 8 WAT and 12 to 16 WAT. Total uptake of N, P and K in optimal and high temperature conditions was $18.4g\;plant^{-1}$ and 17.6 for N, 4.7 and 5.1 for P, and 37.7 and 36.2 for K, respectively, and the pattern of monthly N uptake between optimal and high temperatures was greater in early growth stage, whereas was greater in mid growth stage in both P and K. Thus, this study suggests that moderately high temperature influences much greater to photosynthesis and carbohydrate production than plant biomass and mineral uptake. On the basis of the present result, it is required to indentify analysis of respiration rates from plant and soil by constantly increasing temperature conditions and field studies where elevated temperatures are monitored and manipulated.

• Journal of Advanced Marine Engineering and Technology
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• 제36권1호
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• pp.185-194
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• 2012

비교적 접근이 용이한 육상 및 천해에서 석유의 생산은 이미 완료되었거나 대부분이 진행 중이기에 석유 생산 지역이 점차 심해로 확장되고 있다. 심해와 같은 저온환경에서 석유생산이 이루어지면 석유의 부존환경과 다른 온도와 압력에 노출되어 생산시스템에 다양한 고형물이 집적되어 석유가 안정적으로 생산되지 못하게 하는 유동안정성 확보 문제가 발생할 수 있다. 고형물 중 왁스는 온도에 민감하기 때문에 왁스의 집적을 예측하고 제어하기 위해서는 오일 내 왁스가 석출되기 시작하는 온도인 왁스생성 온도의 정확한 측정이 요구되나 표준화된 측정법이 존재하지 않아 다양한 방법이 제안되고 있는 실정이다. 이 연구에서는 점도측정법, 시차주사열량법, 필터막힘점측정법, 압력필터측정법을 적용하여 불투명 오일의 왁스생성온도를 측정하고, 이를 검증하기 위해 고형물생성유도장치와 가스크로마토크래피를 이용하였다. 이 연구결과를 통해 오일특성에 따른 왁스생성온도 실험법을 체계화한다면 불투명 오일의 왁스생성온도 측정법 표준화하는데 주요자료로 이용할 수 있어 왁스에 의한 유동안정성 확보 문제를 예측하고 해결하는데 활용될 수 있을 것이다.

• 한국토양비료학회지
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• 제49권6호
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• pp.699-704
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• 2016

An estimation of optimal requirement of additional N by cropping pattern and growth stage is very important for greenhouse watermelon. The objectives of this study were to estimate an amount of optimal additional N based on growth, N uptake and yield of watermelon. In order to achieve these goals, we performed the study at farmer's greenhouse with a fertigation system and watermelon was cultivated three times (spring, summer and autumn) in 2015. The levels of additional N were set up with x0.5, x0.75, x1.0 and x1.5 of the $NO_3$-N-based soil-testing N supply for watermelon cultivation. The trends of growth and N uptake of watermelon markedly differed from cropping pattern; spring (sigmoid), summer and autumn (linear). The yield of watermelon was the highest at summer season and followed by autumn and spring. Also, the x1.5N showed a significantly higher yield compared to other N treatments. On the basis of growth, N uptake and yield of watermelon, we estimated an optimal level of additional N by cropping pattern and growth stage as follows; 1) spring (transplanting ~ 6 WAT : 6 ~ 14 WAT : 14 ~ harvest = 5 : 90 : 5%), summer (transplanting ~ 4 WAT : 4 ~ 8 WAT : 8 ~ harvest = 25 : 50 : 25%) and autumn (transplanting ~ 4 WAT : 4 ~ harvesting : 50 : 50%). In conclusion, nutrient management, especially N, based on cropping pattern and growth stage was effective for favorable growth and yield of watermelon.

• 에너지공학
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• 제24권2호
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• pp.1-8
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• 2015

석유의 생산 과정에서 발생하는 왁스(wax)와 같은 고형물의 생성 및 집적은 유동안정성 확보(flow assurance) 문제를 발생시켜 안정적인 석유 생산을 방해할 수 있으며, 현장에서는 이를 해결하는데 많은 시간 소모와 경제적 손실이 발생할 수 있다. 왁스집적 문제는 왁스가 생성되기 시작하는 온도인 왁스생성온도 이하의 조건에서만 발생하므로 왁스집적 문제를 예측하고 제어하기 위해서는 오일의 왁스생성온도를 사전에 필수적으로 파악해야한다. 컨덴세이트와 같은 투명 오일의 왁스생성온도는 광학적 기술인 표준측정법이 적용되는 반면 대부분의 현장에서 생산되는 불투명 오일에는 적용이 어려운 한계점이 있다. 이 연구에서는 3가지의 투명 오일시료에 열유량 변화 분석, 점도 변화 분석, 밀도 변화 분석 기법을 적용하여 파악한 왁스생성온도와 표준기법으로 측정한 값과 비교하여 밀도 변화 분석 기법이 가장 신뢰도가 높은 것을 확인하였고 이 기법을 2종류의 불투명 오일시료에 적용하여 왁스생성온도를 결정하였다.

• 한국응용곤충학회지
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• 제47권4호
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• pp.401-405
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• 2008

배추에서 배추흰나비의 요방제수준을 설정하기 위해 온실 및 포장에서 실험을 수행하였다. 예비실험으로서 배추흰나비 2령충을 다섯 밀도 수준 접종하고 피해정도를 조사하였다. 배추 포기당 유충 1마리 접종에 의한 잎피해면적은 정식3주차 배추에서 섭식 1주일후 657.7 $mm^2$, 정식 6주차 배추에서 섭식 2주일후 2495.8 $mm^2$로 나타났다. 포장실험에서는 배추흰나비 유충 1-7마리를 시험구(배추 20주)에 접종하였다. 3주간 섭식후, 접종 유충 밀도(X)에 따른 수량감소율(Y)은 다음 두가지 식, 즉 정식 3주차 배추는 Y=1.7640X-0.3049 ($R^2$=0.9901), 정식 6주차 배추는 Y=1.0305X-0.2976 ($R^2$=0.9398)로 각각 추정되었다. 접종한 유충밀도와 배추 피해지수간의 상관성을 바탕으로, 수량 감소율(gain threshold, 수익역치) 5%로 전제한 배추흰나비 2령 유충의 요방제수준은 정식 3주차 배추에서 20주당 3.0마리, 정식 6주차 배추에서 20주당 5.1마리로 추정되었다.