• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.171-177
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• 2022

The precise homeostatic regulation of local auxin accumulation in xylem precursors of cambium stem cell tissues is one of the most important mechanisms for plant vascular patterning and radial secondary growth. Walls are thin (WAT1), a novel intracellular auxin transporter, contributes directly to the auxin accumulation maxima in xylem precursors. According to recent research, the auxin signaling activated pathway-related gene network was significantly enriched during the secondary growth of Panax ginseng storage roots. These imply that during P. ginseng root secondary growth, specific signaling mechanisms for local auxin maxima in the vascular cambial cells are probably triggered. This study identified four WAT1-like genes, PgWAT1-1/-2 and PgWAT2-1/-2, in the P. ginseng genome. Their expression levels were greatly increased in nitratetreated storage roots stimulated for secondary root growth. PgWAT1-1 and PgWAT2-1 were similar to WAT1 from Arabidopsis and tomato plants in terms of their subcellular localization at a tonoplast and predicted transmembrane topology. We discovered that overexpression of PgWAT1-1 and PgWAT2-1 was sufficient to compensate for the secondary growth defects observed in slwat1-copi loss of function tomato mutants. This critical information from the PgWAT1-1 and PgWAT2-1 genes can potentially be used in future P. ginseng genetic engineering and breeding for increased crop yield.

• Food Science and Industry
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• v.50 no.1
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• pp.26-35
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• 2017

Obesity is a worldwide problem that is associated with metabolic disorders. Obesity is caused by the accumulation of an abnormal amount of body fat in adipose tissue. Adipose tissue is a major metabolic organ, and it has been classified as either white adipose tissue (WAT) or brown adipose tissue (BAT). WAT and BAT are characterized by different anatomical locations, morphological structures, functions, and gene expression patterns. WAT is mainly involved in the storage and mobilization of energy in the form of triglycerides. On the other hand, BAT specializes in dissipating energy as heat through uncoupling protein-1 (UCP-1)-mediated non-shivering thermogenesis. Novel type of brown-like adipocyte within WAT called beige/brite cells was recently discovered, and this transdifferentiation process is referred to as the "browning" or "britening" of WAT. Recently, Brown fat and/or browning of WAT have been highlights as a new therapeutic target for treatment of obesity and its related metabolic disorders. Here, we describe recent advances in the study of BAT and browning of WAT, focusing on proteomic approaches.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.3
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• pp.297-302
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• 1996

The effect of application rate of pig or chicken manure on the seasonal change of tomato(Minicarol) rhizosphere microflora was investigated by field experiment were surface soil(16cm) mixed with manures 1 weeks before transplanting. 1. Rhizobacteria population of control plot decreased 5 weeks after transplanting(WAT) than 1 WAT and 1.5~2 times higher colony counting was found in DNB(Diluted nutrient broth medium) than NB(Nutrient broth medium) at 15 WAT. 2. Rhizobacteria population at 1 WAT inereased in NB medium with the application rate of both manures but decreased in DNB with chicken manure. Colony counting in NB of 60 and 120 mg/ha treatment was 100 times higher than that of the control. However, rhizobacteria in DNB at 15 WAT(harvest stage) was much higher than that of NB. 3. Application rate did not affect fungi population 1 WAT in pig manure but decresed in chicken manure. At 15 WAT fungi population was 2 times higher than 1 WAT with chieken manure and highest in 30 mg/ha but with pig manure decreased with rate than the control. 4. Actinomycetes population at 1WAT was not different among pig manure rates and decreased with chicken manure than control. At 15 WAT population increased in all manure rates. especially in 10 mg/ha pig manure and 30 mg/ha chicken manure 4 times that 1 WAT.

• Journal of Ginseng Research
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• v.40 no.2
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• pp.141-150
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• 2016

Background: Adipocyte-macrophage communication plays a critical role regulating white adipose tissue (WAT) inflammatory gene expression. Because WAT inflammation contributes to the development of metabolic diseases, there is significant interest in understanding how exogenous compounds regulate the adipocyte-macrophage crosstalk. An aqueous (AQ) extract of North American (NA) ginseng (Panax quinquefolius) was previously shown to have strong inflammo-regulatory properties in adipocytes. This study examined whether different ginseng extracts influence adipocyte-macrophage crosstalk, as well as WAT inflammatory gene expression. Methods: The effects of AQ and ethanol (EtOH) ginseng extracts ($5{\mu}g/mL$) on adipocyte and macrophage inflammatory gene expression were studied in 3T3-L1 and RAW264.7 cells, respectively, using real-time reverse transcription polymerase chain reaction. Adipose tissue organ culture was also used to examine the effects of ginseng extracts on epididymal WAT (EWAT) and inguinal subcutaneous WAT (SWAT) inflammatory gene expression. Results: The AQ extract caused significant increases in the expression of common inflammatory genes (e.g., Mcp1, Ccl5, Tnf-${\alpha}$, Nos2) in both cell types. Culturing adipocytes in media from macrophages treated with the AQ extract, and vice versa, also induced inflammatory gene expression. Adipocyte Ppar-${\gamma}$ expression was reduced with the AQ extract. The AQ extract strongly induced inflammatory gene expression in EWAT, but not in SWAT. The EtOH extract had no effect on inflammatory gene expression in either both cell types or WAT. Conclusion: These findings provide important new insights into the inflammo-regulatory role of NA ginseng in WAT.

• Molecules and Cells
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• v.47 no.2
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• pp.100030.1-100030.14
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• 2024

Both brown and white adipose tissues (BAT/WAT) are innervated by the peripheral nervous system, including efferent sympathetic nerves that communicate from the brain/central nervous system out to the tissue, and afferent sensory nerves that communicate from the tissue back to the brain and locally release neuropeptides to the tissue upon stimulation. This bidirectional neural communication is important for energy balance and metabolic control, as well as maintaining adipose tissue health through processes like browning (development of metabolically healthy brown adipocytes in WAT), thermogenesis, lipolysis, and adipogenesis. Decades of sensory nerve denervation studies have demonstrated the particular importance of adipose sensory nerves for brown adipose tissue and WAT functions, but far less is known about the tissue's sensory innervation compared to the better-studied sympathetic nerves and their neurotransmitter norepinephrine. In this review, we cover what is known and not yet known about sensory nerve activities in adipose, focusing on their effector neuropeptide actions in the tissue.

• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.291-298
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• 2014

Plastic film houses are directly associated with increases in plant growth and yield of vegetable crops through a year round cultivation, however, at the same time temperature stresses are one of fates which are difficult to avoid during crop growth. The objective of this study was to examine the translocation and distribution of minerals (N, P, K) and carbohydrates as well as seasonal fluctuation of mineral uptake and carbohydrate production in cucumber plant grown under moderately high temperature. The temperature treatments consisted of 2-layers film houses (optimal temp.) and 3-layers (high temp.). Shoot growth of cucumber plants were linearly increased until 14 weeks after transplanting (WAT) without any significant difference between both temperatures, and the slowdown was observed from 16 WAT. The level of soluble sugar and starch was slightly greater in optimal temperature compared to the high. Cumulative accumulation of soluble sugar was significantly different before and after 12 WAT in both treatments, whereas starch level represented a constant increase. Monthly production of soluble sugar reached the peak between 12 to 16 WAT, and starch peaked between 4 to 8 WAT and 12 to 16 WAT. Total uptake of N, P and K in optimal and high temperature conditions was $18.4g\;plant^{-1}$ and 17.6 for N, 4.7 and 5.1 for P, and 37.7 and 36.2 for K, respectively, and the pattern of monthly N uptake between optimal and high temperatures was greater in early growth stage, whereas was greater in mid growth stage in both P and K. Thus, this study suggests that moderately high temperature influences much greater to photosynthesis and carbohydrate production than plant biomass and mineral uptake. On the basis of the present result, it is required to indentify analysis of respiration rates from plant and soil by constantly increasing temperature conditions and field studies where elevated temperatures are monitored and manipulated.

• Journal of Advanced Marine Engineering and Technology
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• v.36 no.1
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• pp.185-194
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• 2012

Deepwater oil is becoming more attractive because most onshore and shallow water oil is developing or developed. With the on-going trend to deepwater oil developments, flow assurance problems which prevent oil flow from reservoir to processing facilities are becoming an issue because deposited material can be occurred in case oil is exposed to very different environment from reservoir. Wax deposition which is one of flow assurance problems can be a major technical and economic issue because it is very sensitive to temperature. In order to predict and mitigate wax problems, the precise measurement of wax appearance temperature (WAT) which is the starting temperature of wax precipitation is very important. Various methods have been suggested for WAT measurement of opaque oil because there is no standard method for opaque oil. In this study, the WAT of opaque oil samples was measured using viscosity measurement method, differential scanning calorimetry, filter plugging method, and pressurized filter plugging method. Wax deposition test and high temperature gas chromatography analysis were applied to verify measured WAT. As a result of study, the WAT of opaque oils was successfully measured and verified. If WAT measurement methods of opaque oil related to oil characteristics is systematized using the results of this study, it can be a valuable tool for WAT measurement of opaque oil and flow assurance related to wax deposition.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.699-704
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• 2016

An estimation of optimal requirement of additional N by cropping pattern and growth stage is very important for greenhouse watermelon. The objectives of this study were to estimate an amount of optimal additional N based on growth, N uptake and yield of watermelon. In order to achieve these goals, we performed the study at farmer's greenhouse with a fertigation system and watermelon was cultivated three times (spring, summer and autumn) in 2015. The levels of additional N were set up with x0.5, x0.75, x1.0 and x1.5 of the $NO_3$-N-based soil-testing N supply for watermelon cultivation. The trends of growth and N uptake of watermelon markedly differed from cropping pattern; spring (sigmoid), summer and autumn (linear). The yield of watermelon was the highest at summer season and followed by autumn and spring. Also, the x1.5N showed a significantly higher yield compared to other N treatments. On the basis of growth, N uptake and yield of watermelon, we estimated an optimal level of additional N by cropping pattern and growth stage as follows; 1) spring (transplanting ~ 6 WAT : 6 ~ 14 WAT : 14 ~ harvest = 5 : 90 : 5%), summer (transplanting ~ 4 WAT : 4 ~ 8 WAT : 8 ~ harvest = 25 : 50 : 25%) and autumn (transplanting ~ 4 WAT : 4 ~ harvesting : 50 : 50%). In conclusion, nutrient management, especially N, based on cropping pattern and growth stage was effective for favorable growth and yield of watermelon.

• Journal of Energy Engineering
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• v.24 no.2
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• pp.1-8
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• 2015

Wax deposition hinders oil flow assurance. Huge amount of money and time were required for mitigation of wax deposition in the oil field. For prediction and mitigation of wax deposition problem, Wax Appearance Temperature(WAT), which is the temperature at which the first wax crystals start to form, needs to be measured in advance. There is a standard method which is optical way to measure the WAT of transparent oil. However, standard method cannot be applied to opaque oil which is common produced oil in the field. In this study, WAT of three transparent oil samples were measured using heat flux variation analysis, viscosity variation analysis and density variation analysis, and compared with WAT measured by standard method. As a result, WAT measured by density variation analysis is the more reliable than heat flux variation analysis and viscosity variation analysis. WAT of two opaque oils were measured using density variation analysis.

• Korean journal of applied entomology
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• v.47 no.4
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• pp.401-405
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• 2008

This study was conducted to estimate the control thresholds (CTs) of imported cabbage worm, Artogeia rapae L., injuring Chinese cabbage. The second instar larvae of A. rapae were inoculated with five density levels on each Chinese cabbages transplanted three weeks earlier under greenhouse condition, and checked injury rates after allowing their feeding for one week and two weeks, respectively. The average leaf area consumed by single larvae was 657.7 $mm^2$ in plots inoculated at three weeks after transplanting (WAT) and 2495.8 $mm^2$ in plots at 6-WAT, respectively. In the field experiment, different numbers of A. rapae ranged from one to seven larvae were inoculated on 20 plants. The percent yield reduction (Y) of Chinese cabbage infested by different densities of A. rapae (X) for a three-week period was estimated by the following equation; (1) Y=1.764X-0.3049 ($R^2$=0.9901) in plots inoculated at 3-WAT; and (2) Y=1.0305X-0.2976 ($R^2$=0.9398) in plots inoculated at 6-WAT. Based on the relationships between the densities of A. rapae larvae and the yield index of Chinese cabbage, the number of second instar larvae which caused 5% loss of yield (gain threshold proposed by Japan), was estimated as 3.0 per 20 plants for the 3-WAT and 5.1 for the 6-WAT.