• Title/Summary/Keyword: W/Mo

Search Result 760, Processing Time 0.032 seconds

Efficient Production of ε-Poly-L-Lysine by Streptomyces ahygroscopicus Using One-Stage pH Control Fed-Batch Fermentation Coupled with Nutrient Feeding

  • Liu, Sheng-Rong;Wu, Qing-Ping;Zhang, Ju-Mei;Mo, Shu-Ping
    • Journal of Microbiology and Biotechnology
    • /
    • v.25 no.3
    • /
    • pp.358-365
    • /
    • 2015
  • ε-Poly-L-lysine (ε-PL) is a homopolymer of L-lysine molecules connected between the epsilon amino and alpha carboxyl groups. This polymer is currently used as a natural preservative in food. Insufficient biomass is a major problem in ε-PL fermentation. Here, to improve cell growth and ε-PL productivity, various nitrogen-rich nutrients were supplemented into flask cultures after 16 h cultivation, marking the onset of ε-PL biosynthesis. Yeast extract, soybean powder, corn powder, and beef extract significantly improved cell growth. In terms of ε-PL productivity, yeast extract at 0.5% (w/v) gave the maximum yield (2.24 g/l), 115.4% higher than the control (1.04 g/l), followed by soybean powder (1.86 g/l) at 1% (w/v) and corn powder (1.72 g/l) at 1% (w/v). However, supplementation with beef extract inhibited ε-PL production. The optimal time for supplementation for all nutrients examined was at 16 h cultivation. The kinetics of yeast-extract-supplemented cultures showed enhanced cell growth and production duration. Thus, the most commonly used two-stage pH control fed-batch fermentation method was modified by omitting the pH 5.0-controlled period, and coupling the procedure with nutrient feeding in the pH 3.9-controlled phase. Using this process, by continuously feeding 0.5 g/h of yeast extract, soybean powder, or corn powder into cultures in a 30 L fermenter, the final ε-PL titer reached 28.2 g/l, 23.7 g/l, and 21.4 g/l, respectively, 91.8%, 61.2%, and 45.6% higher than that of the control (14.7 g/l). This describes a promising option for the mass production of ε-PL.

Development of Creep Properties Evaluation Technique for Steel Weldment of Power Plant (발전설비 강 용접부의 크리프 특성 평가 기술 개발)

  • Lee, Dong-Hwan;Jeoung, Young-Hun;Baek, Seung-Se;Ha, Jeong-Soo;Song, Gee-Hook;Lee, Song-In;Yu, Hyo-Sun
    • Proceedings of the KSME Conference
    • /
    • 2001.11a
    • /
    • pp.180-185
    • /
    • 2001
  • In the life assessment for plant structural component, the research on deterioration of toughness and material properties occurred in weldments has been considered as very important problems. In general, the microstructures composed in weldments are hugely classified with weld metal(W.M), fusion line(F.L), heat affected zone(HAZ), and base metal(B.M). It has been reported that the creep characteristics on weldments having variable microstructures could be unpredictably changed. Furthermore, it is also known that HAZ adjacent to F.L exhibits the decreased creep strength compared to those in base or weld metals, and promotes the occurrence of Type III and Type IV cracking due to the growth of grains and the coarsening carbides precipitated in ferritic matrix by welding and PWHT processes. However, the lots of works reported up to date on creep damage in power plant components have been mostly conducted on B.M and the creep properties on a localized microstructures in weldments have not as yet been throughly investigated. In this paper, for various microstructures such as coarse grain HAZ(CGHAZ), W.M and B.M in X20CrMoV121 steel weldment, the small punch-creep(SP-Creep) test using miniaturized specimen(t=0.5mm, 0.25mm) is performed to investigate a possibility for creep characteristics evaluation.

  • PDF

A New Potato Cultivar "Early Valley", with High Yield and Early Maturity

  • Lim, H.T.;Dhital, S.P.;Khu, D.M.;Choi, S.P.;Kang, C.W.;Kim, T.J.;Mo, H.S.;Hwang, W.N.;Lee, W.J.
    • Korean Journal of Breeding Science
    • /
    • v.41 no.1
    • /
    • pp.61-67
    • /
    • 2009
  • "Early Valley", is an early maturing potato cultivar with high yield potential. "Early Valley" is a clonal selection resulting from the cross between 'Suncrisp' and 'A87109-10'. It has medium plant height and light green foliage. "Early Valley" has medium flowering habit and white flowers. Tubers are smooth, yellow skin, light yellow flesh, round tuber shape, medium eye depth, and medium dormancy and good keeping quality. It has stable yield under wide range of climatic conditions. "Early Valley" is resistance to late blight, but moderately susceptible to common scab and hollow heart. This cultivar is also resistant to potato rotting at harvesting during the raining season. "Early Valley" has high level of antioxidant activity (about three times higher) and vitamin C (higher by 40%) than the 'Superior'. This cultivar has high level of tuber uniformity and capable of yielding 36.56 t/ha which is 17.07% higher than the control potato cultivar 'Superior' under optimum agronomical practices.

Effect of Compression Treatment on Characteristics of Solidified Cow Manure Fuel (우분 압착 처리가 우분 고체연료 특성에 미치는 영향)

  • Jeong, Kwang-Hwa;Kim, Jung-Kon;Lee, Dong-Jun;Cho, Won-Mo
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.24 no.2
    • /
    • pp.67-74
    • /
    • 2016
  • This study was purposed to evaluate the characteristic changes of the solidified livestock manure fuel(SLMF) through the application of compression treatment process. The compression process led to an increase of VS/TS ratio (Volatile solids/Total solids ratio) and moisture removal effect of SLMF. The amount of leachate withdrawn from dairy cattle manure and Hanwoo manure by compression were 21~26%(w/w) and 15~20%(w/w), respectively. The specific gravity of the leachate of dairy cattle manure and Hanwoo manure were 1.01 and 0.99, respectively. The dehydrated cow manure was easily processed into ball-shaped solidified fuel. The drying time of the SLMF was proportional to the diameter of the solidified fuel. The highest heating value was observed in diameter range of 10~15mm SLMF. It is concluded that the higher heating value of 10~15mm SLMF was related with the amount of fibrous matter contained in the SLMF.

Quality Properties of Baechu kimchi treated with Black Panax ginseng Extracts during Fermentation at Low Temperature (흑삼추출액을 첨가한 배추김치의 저온 저장 중의 품질 특성)

  • Mo, Eun-Kyoung;Kim, Seung-Mi;Yun, Beom-Sik;Yang, Sun-A;JeGal, Sung-A;Choi, Young-Sim;Ly, Sun-Yung;Sung, Chang-Keun
    • Food Science and Preservation
    • /
    • v.17 no.2
    • /
    • pp.182-189
    • /
    • 2010
  • To develop a new functional kimchi with cognition-enhancing properties, black Panax ginseng extract (0.5-5%, w/w) was added to a baechu kimchi preparation and the mixture stored at $4^{\circ}C$ for 30 days. Compared with control kimchi, the L values of ginseng-treated material were significantly decreased, but the a and b values were increased. The hardness value of ginseng-treated kimchi was significantly higher than that of control material from the $20^{th}$ day of storage. The edibility period of baechu kimchi treated with ginseng was prolonged by approximately 15 days compared with control kimchi. This resulted from decreases in the numbers of lactic acid bacteria and yeasts during the final stages of fermentation in ginseng-treated material. Inhibition of acetylcholinesterase activity by ginseng-treated kimchi was 2-fold higher than that of control material. A strong ginseng flavor and a bitter taste were evident in kimchi treated with 5% (w/w) ginseng, and sensory quality was thus decreased compared with control material. It was concluded that an appropriate concentration of black ginseng extract was 3% (w/w) in preparation of kimchi with a cognition-enhancing effect.

Development of Tree Stem Weight Equations for Larix kaempferi in Central Region of South Korea (중부지역 일본잎갈나무의 수간중량 추정식 개발)

  • Ko, Chi-Ung;Son, Yeong-Mo;Kang, Jin-Taek;Kim, Dong-Geun
    • Journal of Korean Society of Forest Science
    • /
    • v.107 no.2
    • /
    • pp.184-192
    • /
    • 2018
  • In this study was implemented to develop tree stem weight prediction equation of Larix kaempferi in central region by selecting a standard site, taking into account of diameter and position of the local trees. Fifty five sample trees were selected in total. By utilizing actual data of the sample trees, 11 models were compared and analyzed in order to estimate four different kinds of weights which include fresh weight, ovendry outside bark weight, ovendry inside bark weight and merchantable weight. As to estimate its weight, the study has classified its model according to three parameters: DBH, DBH and height, and volume. The optimal model was chosen by comparing the performance of model using the fit index and standard error of estimate and residual distribution. As a result, the formula utilizing DBH (Variable 1) is $W=a+bD+cD^2$ (3) and its fit index was 90~92%. The formula for DBH and height (Variable 2) is $W=aD^bH^C$ (8) and its fit index was 97~98%. In summation, Variable 2 model showed higher fitness than Variable 1 model. Moreover, fit index of formula for total volume and merchantable volume (W=aV) showed high rate of 98~99%, as well as resulting 7.7-17.5 with SEE and 8.0-10.0 with CV(%) which lead to predominately high fitness in conclusion. This study is expected to provide information on weights for single trees and furthermore, to be used as a basic study for weight of stand unit and biomass estimation equations.

A study on the Frequency Dependence of Dynamic Pyroelectric Properties for $Pb_{l-x}La_{x}Ti_{l-x/4}O_3$ (x=0.1) (PLT(10)) Ferroelectric Thin Film ($Pb_{l-x}La_{x}Ti_{l-x/4}O_3$ (x=0.1) (PLT(10)) 강유전체 박막에서 동적 초전특성의 주파수의존성에 관한 연구)

  • 차대은;장동훈;강성준;윤영섭
    • Journal of the Institute of Electronics Engineers of Korea SD
    • /
    • v.39 no.12
    • /
    • pp.1008-1015
    • /
    • 2002
  • The fabricated La-modified lead titanate (PLT) thin film without poling treatment was investigated for modulation frequency dependence of pyroelectric properties by the dynamic method. $Pb_{l-x}La_{x}Ti_{l-x/4}O_3$(x=0.1) (PLT(10)) thin film haying 10 mol% La content was deposited on a Pt/$TiO_{x}$/$SiO_2$/Si substrate by sol-gel method. The PLT(10) thin film exhibits a relatively excellent dielectric property. The pyroelectric coefficient (p) of the PLT(10) thin film is 6.6 x $10^{-9}C$$textrm{cm}^2$$.$K without frequency dependence. The figure of merits for the voltage responsivity and specific detectivity are 1.03 x $10^{-11}C$.cm/J and 1.46 x $10^{-10}C$.cm/J, respectively The PLT(10) thin film has voltage responsivity (RV) of 5.IS V/W at 8 Hz. Noise equivalent power (NEP) and specific detectivity ($D^{*}$) of the PLT(10) thin film are 9.93 x $10^{-8}$W/$Hz^{1/2}$ and 1.81 x $10^{6}$cm.$Hz^{1/2}$/W at the same frequency of 100 Hz,, respectively The results means that PLT thin film having 10 mol% La content is suitable for the sensing materials of pyroelectric IR sensors.

Designed of rPP/d2w®/ZnO Nanocomposite Flexible Film for Food Packaging and Characterization on Mechanical and Antimicrobial Properties (산화분해촉매를 함유한 rPP/ZnO 나노컴포지트 유연식품포장필름 제조 및 물성 특성 연구)

  • Lee, Jin-kyoung;Gil, Bo-min;Lee, Dong-jin;Lee, Ik-mo
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
    • /
    • v.24 no.1
    • /
    • pp.1-11
    • /
    • 2018
  • In this study, pro-oxidant($d2w^{(R)}$) and rPP/ZnO nanocomposite flexible films for food packaging were prepared, and their mechanical and antimicrobial properties were investigated. As a result, the carbonyl index and hydroxyl index increased with exposured time to heat and UV rays. Surface analysis showed that the addition of zinc oxide improved the dispersibility and compatibility of the polymer, so that the surface of the composite film was smooth and the zinc oxide particles were smaller than the compared film. And it kept the physical properties by heat and UV ray blocking effect, and it worked to reduce decomposition. In the antimicrobial activity test, the microbial reduction rate was 3 logs or more at the use concentration of zinc oxide. The tensile strength was increased and the elongation was decreased. Oxidative degradability of multi-layered film in UV exposured for 72 hours, the molecular weight of the film decreased by 75.6%, 1,294 g/mol Mn and 5,920 g/mol Mw. In the safety analysis of food packaging materials, we obtained that are in standard of polypropylene, a food contact material of domestic law.

Low voltage Low power OTAs using bulk driven in 0.35㎛ CMOS Process (0.35㎛ CMOS 공정에서 벌크 입력을 사용한 저전압 저전력 OTAs)

  • Kang, Seong-Ki;Jung, Min-Kyun;Han, Dae-Deok;Yang, Min-Jae;Yoon, Eun-Jung;Yu, Chong-Gun
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2015.10a
    • /
    • pp.451-454
    • /
    • 2015
  • This paper introduces 3 type of OTAs with $0.35-{\mu}m$ standard CMOS technology for Low-Power, Low-Voltage. The first type is a two-stage OTA designed to operate with a 1-V VDD and it has $1.774{\mu}W$ low power consumption. All transistors are operating in strong inversion. It takes Gm-Enhancement techniques to compensate gm, which is lowered by Bulk-Driven technique and has an Wide swing current mirror for low voltage operation and a Class-A output. The second type is a Two-stage OTA designed to operate with a 0.8-V VDD and It has 52nW low power consumption and 112dB high gain. The current mirror uses Composite Transistor binding Gates of two MOSFET to raise Rout which is similar with cascode structure. The third type is a Two-stage OTA designed to operate with a 0.6-V VDD and It has 160nW low power consumption and 72dB high gain. It takes Level Shift technique by Common Gate structure to amplify signals without additional bias voltage at second stage.

  • PDF

The Effect of Niobium on Wear and Friction Characteristics of High Speed Steel by Powder Metallurgy (분말고속도공구강의 마찰마모특성에 미치는 Nb의 영향)

  • 이한영;백금주;김용진;배종수;홍성현
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
    • /
    • 1999.06a
    • /
    • pp.14-19
    • /
    • 1999
  • In order to evaluate the effect of Nb on wear properties of high speed steel by powder metallurgy(PM-HSS), niobium-alloyed PM-HSS have been prepared by adding 0%, 1%, 3% and 5%Nb to PM-HSS of 6%W-5%Mo-4%Cr-5%V-5%Co presented in the previous paper. Sliding wear test have been conducted in various sliding speed conditions under the constant pressure using a pin-on-disc type machine. The results of this study shows that the wear resistance of PM-HSS has been increased by the addition of Nb in the range of experimental sliding speed. However, the amount of Nb shows to be unimportant parameter for the improvement of the wear resistance. It may be due to the thermal stability of carbide and high temperature properties of matrix by adding Nb comparing to the case of no addition.

  • PDF