• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.25 no.4
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• pp.89-98
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• 2012

Objective : Photodynamic therapy (PDT) using topical aminolevulinic acid (ALA) has increasingly been used for the treatment of acne vulgaris and several studies have shown its clinical efficacy. However, ALA-PDT needs a relatively long incubation period and is frequently associated with adverse effects. Triptophan and Riboflavin has been introduced as a new photosensitizer for the treatment of acne recently. Method : 11 patients with acne vulgaris were received PDT with tryptophan and riboflavin four to six times over two to three months. The photos of each patient were taken before and after treatment. Result and conclusion : PDT with tryptophan and riboflavin may be an effective and safe treatment for the Acne vulgaris patients.

• Bulletin of the Korean Chemical Society
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• v.31 no.9
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• pp.2715-2716
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• 2010

• CELLMED
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• v.10 no.2
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• pp.15.1-15.9
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• 2020

Introduction: Buthūr Labaniyya (Acne vulgaris) is a multifactorial disorder of the pilosebaceous units characterized by non-inflammatory and inflammatory clinical lesions. Several Unani medications have been used for centuries to treat acne. Objectives: Evaluation of safety and efficacy of Tila-i Muhāsā in patients with acne vulgaris Materials and Methods: This clinical study was conducted in patients with acne vulgaris. Patients applied Tila-i Muhāsā or 5% Benzoyl Peroxide (BPO) once daily for 6 weeks. Assessment of efficacy was carried out by Global Acne Grading System (GAGS) and Patient Global Assessment (PGA) scores. In addition, overall severity of acne was evaluated on a 5-point Cook's acne grading scale using photographic standards. Assessment of safety was performed through adverse drug reactions, local dermal tolerability, vital signs, and routine laboratory investigations. Results: A total of 60 patients (30 in each group) completed 6 weeks of treatment. The mean percentage reduction in GAGS score at 6 weeks from baseline in Unani group (66.97%) and BPO group (59.09%) was statistically significant (P<0.0001). After 6 weeks of therapy, the mean percentage reduction in PGA score compared to baseline in Unani group (57.44%) and BPO group (50.23%) was statistically significant (P<0.0001). No serious adverse events were reported in both groups; however, mild adverse events occurred more frequently in BPO group (30%) compared to Unani group (10%). Conclusion: Tila-i Muhāsā was found to be effective and safe in the treatment of acne vulgaris. However, further clinical studies with larger sample size and longer duration of therapy need to be conducted.

• Environmental Engineering Research
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• v.25 no.2
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• pp.135-146
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• 2020

This study was aimed to investigate the possibility of using raw and anaerobically-digested piggery wastewater as culture media for a green microalga Chlorella vulgaris (C. vulgaris). Due to high concentration of ammonia and dark color, the microalga did not grow well in this wastewater. In order to solve this problem, air stripping and NaOCl-treatment were applied to reduce the concentration of NH₃-N and the color intensity from the wastewater. Algal growth was monitored in terms of specific growth rate, biomass productivity, and nutrient removal efficiency. As a result, C. vulgaris grew without any sign of inhibition in air-stripped and 10-folds diluted anaerobically-digested piggery wastewater with enhanced biomass productivity of 0.57 g/L·d and nutrient removal of 98.7-99.8% for NH₃-N and 41.0-62.5% for total phosphorus. However, NaOCl-treatment showed no significant effect on growth of C. vulgaris, although dark color was removed greatly. Interestingly, despite that the soluble organic concentration after air stripping was still high, the biomass productivity was 4.4 times higher than BG-11. Moreover, air stripping was identically effective for raw piggery wastewater as for anaerobic digestate. Therefore, it was concluded that air stripping was a very effective method for culturing microalgae and removing nutrients from raw and anaerobically-digested piggery wastewaters.

• Environmental Engineering Research
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• v.18 no.4
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• pp.235-239
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• 2013

In the present investigation, the efficiency of Chlorella vulgaris (C. vulgaris) was evaluated for the removal of ammonia-nitrogen from wastewater. Eight different wastewater samples were prepared with varied amounts of $NH_4-N$ concentrations from 15.22 to 205.29 mg/L. Experiments were conducted at pH $7.5{\pm}0.3$, temperature $25^{\circ}C{\pm}1^{\circ}C$, light intensity $100{\mu}E/m^2/s$, and dark-light cycles of 8-16 hr continuously for 8 days. From the results, it was found that $NH_4-N$ was completely removed by C. vulgaris, when the initial concentration was between 5.22-25.24 mg/L. However, only 50% removal was obtained when the $NH_4-N$ concentration was 85.52 mg/L, which further decreased to less than 32% when the $NH_4-N$ concentration exceeded 105.43 mg/L. The further influence of nitrogen on chlorophyll was studied by various $NH_4-N$ concentrations. The maximal value of chlorophyll a (Chl a) content was found to be 19.21 mg/L for 65.79 mg/L $NH_4-N$ concentration, and the maximum specific $NH_4-N$ removal rate of 1.79 mg/mg Chl a/day was recorded at an $NH_4-N$ concentration of 85.52 mg/L. These findings demonstrate that C. vulgaris could potentially be employed for the removal of $NH_4-N$ from wastewater.

• The Korean Journal of Zoology
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• v.33 no.2
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• pp.222-230
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• 1990

The karyotypes of Korean Sciunis vulgaris coreas and Tamias sibiricus asiaticus were analyzed by conventional Giemsa staining and C-banding method. The diploid chromosome number (2n) of Sciunis vulgaris coreae 40 consisting of 6 metacentric, 8 submetacentric, 3 subtelocentric and 2 telocentric autosome pairs, submetacentric X and acrocentric or subtelocentric Y chromosome. The arm number (NF) of this species was obtained as 72, excluding the gonosomal arms. Tamias sibiricus asiaticus has a 2n of 38. The karyotype was represented by 3 metacentric, 4 submetacentric, 5 subtelocentric and 6 telocentric autosome paits and 2 sex chromosome. The X chromosome was submetacentric chromosome and the Y was the smallest chromosome with a median. The NF was 60. In S. vulgaris coreae constitutive heterochromatins were observed at the centromeres and telomeres. Constitutive heterochnomatins of T sibiricus asiaticus were primarily observed at the centromeres. These results suggested that non-Robensonian reanagenents and distribution of constitutive heterochromatin played an imporiant role in karyological differentiation of these species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.5
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• pp.728-733
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• 2023

The diet composition of octopus Octopus vulgaris was studied using 392 specimens collected from January to December 2020 in the coastal waters of Yeosu, Korea. The body weight ranged from 86.4 to 3,645.4 g. The most important prey item in the diet of O. vulgaris was decapods, which constituted 43.9% in ranking index (%RI). The second largest prey item was Pisces (%RI=27.9%), and cephalopods (%RI=21.8%) constituted the third largest prey item. The average trophic level of O. vulgaris was 3.97±0.59. Ontogenetic changes were significant among size classes (<400 g, 400-800 g, ≥800 g). The small size class (<400 g) mainly fed on decapods, whereas the medium (400-800 g) and large size (≥800 g) classes mainly fed on cephalopods and Pisces, respectively. The dietary composition varied significantly with season.

• The Journal of Dong Guk Oriental Medicine
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• v.6 no.1
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• pp.177-203
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• 1997

Various kinds of medicinal herbs and prescribed herb medicines which have usually been used for treatment of reducing fever, purging intense heat, and detoxication were secreened for antibacterial activity against Proteus vulgaris, S. aureus, and E. faecalis which causing puerperal infection. Minimal inhibitory concentrations of water and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) were determined and using thin-layer chromatography the extracts of Hwangyon and Omiza were separated. The results obtained were as follow : 1. The water-soluble extracts of Keumeunhwa(L. japonica), Chiza(G. jasminoides), Hwangyon(C. japonica), Dansam(S. miltiorrhiza), Omae(P. mume) and Omiza(S. chinensis) showed antibacterial activities against P. vulgaris ; among them, the extracts of Hwangyon, Omae, and Omiza showed high antibacterial activities. The water-soluble extracts of Keumeunhwa(L. japonica), Hwangyon(C. japonica), Hwangkeum(S. baicalensis), Hwangbaek(P. amurense), Dansam(S. miltiorrhiza), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), Gingseng(P. ginseng), and Gamcho(G. uralensis) showed antibacterial activities against S. aureus : among them, the extracts of Hwangyon and Omiza showed high antibacterial activities. The water-soluble extracts of Banggi(C. trilobus), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), and Gamcho(G. uralensis) inhibited the cell growth of E. faecalis : among them, Omae and Omiza showed high antibacterial activities. The extract of Hwangyon(C. Japonica) and Omae(P. mume) showed no inhibition against E. coli. However, the extract of Omiza(S. chinensis) inhibited cell growth of both E. coli and B. subtilis. 2. The water- and ethanol-soluble extract of Hwangyon(C. Japonica) showed antibacterial activities against P. vulgaris and S. aureus, those of Omae(P. mume) against P. vulgaris and E. faecalis, and those of Omiza(S. chinensis) against all species tested, P. vulgaris, S. aureus, and E. faecalis. With the exception that ethanol-soluble extract of Hwangyon(C. japonica) showed much higher antibacterial activity against S. aureus than water-soluble one, antibacterial activities of both water-and ethanol-soluble ones were similar to each other of other two medicinal herbs. 3. When the prescribed herb medicines were tested, Sambohwan showed antibacterial activities against P. vulgaris and S. aureus. No prescribed herb medicine inhibitied the cell growth of E. faecalis. 4. Minimal inhibitory concentrations(MICs) of both water- and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) against P. vulgaris were $2.5mg/m{\ell}$, $10mm{\ell}$, and $20mg/m{\ell}$, respectively. Those of both Hwangyon(C. japonica) and Omiza(S. chinensis) against S. aureus were $1.25mg/m{\ell}$ and $10mg/m{\ell}$, respectively. MICs of water-soluble extracts of Omae(P. mume) and Omiza(S. chinensis) against E. faecalis were $2.5m{\ell}$ and $5m{\ell}$, and those of ethanol-soluble extracts were $5m{\ell}$ and $10m{\ell}$, respectively Except for those of E. faecalis the cell growth of P. vulgaris and S. aureus were inhibited by much lower concentration of ethanol-soluble extracts used. As a result, the antibacterial compounds against P. vulgaris S. aureus, and E. aecalis are contained in the extracts of Hwangyon(C. japonica), Omae(P. mume), Omiza(S. chinensis), the prescribed herb medicine, Sambohwan, and might be used for treatment of puerperal infection. Further study should be carried out to identify which compounds affect the cell growth inhibition of P. vulgaris S. aureus, and E. faecalis.

• The Korean Journal of Pesticide Science
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• v.12 no.1
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• pp.50-56
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• 2008

The purpose of the study was to determine the effects of isoprothiolane, diazinon, butachlor, dimethametryn and molinate in Selenastrum capriconurtum, Scenedesmus subspicatus, Chlorella vufgaris and Nitzschia palea during an exposure period of 72 hours. The study was carried out in according with the OECD Guidelines for Testing of Chemicals No 201 Alga, Growth Inhibition Test. The toxicological responses of isoprothiolane to S. capriconutum, S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ (Median Effective Concentration by growth rate) value, were 5.87, 9.91, $18.55 and 38.79\;mg\;L^{-1}$, respectively. Diazinone to S. capriconutum, S. subspicatus, C. vulgaris and palea expressed as in individual $ErC_{50}$ value, were 10.31, 11.44, >32 and $14.32\;mg\;L^{-1}$, respectively. Butachlor to S. capriconutum, S. subspicatus, C. vulgaris and N. plaea expressed as in individual $ErC_{50}$ value, were 0.002, 0.019, 8.67 and $4.94\;mg\;L^{-1}$, respectively. Dimethamelryn to S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ value, were 0.0071, 0.011, 0.0065 and $0.009\;mg\;L^{-1}$, respectively. Molinate to S. capriconutum, S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ value, were 0.44, 1.26, 48.84 and $28.52\;mg\;L^{-1}$, respectively. The sensitivities of five pesticides were different depending on freshwater alga in the order of S. capriconutum > S. subspicatus > C. vulgaris, N. palea. Highly significant correlation (r=0.9677) based on $ErC_{50}$ were found between S. capriconutum and S. subspicatus.

• Applied Chemistry for Engineering
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• v.26 no.3
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• pp.265-269
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• 2015

Chlorella vulgaris (C. vulgaris) is a spherical unicellular green algae and the diameter ranges from 2 to $10{\mu}m$. C. vulgaris possess nutritional excellence because it contains various functional materials including high protein contents, chlorophyll, carotenoid, and chlorella growth factor (CGF). In order to study effects of mutagen, ethyl methane sulphonate (EMS) was used as a chemical mutagen and some mutants could be obtained. We named 2 type mutants as E14 and E24 obtained after treating with EMS. In the cell growth, growth patterns of mutants were similar to those of the wild type. Chlorophyll contents of E14 and E24 increased up to 99 and 52%, respectively compared to those of the wild type. The carotenoid content of E14 increased to 7%, but the value of E24 decreased 5% compared to that of the wild type. For the lipid contents E24 increased to 23%, while E14 decreased 12% when compared to those of the wild type. As a result, there is no difference between the mutants and wild type in the cell growth, but considering that mutants contains more physiological materials than those of the wild type, we can expect the mutants of C. vulgaris could be used as important high added-value materials.