Abstract
The purpose of the study was to determine the effects of isoprothiolane, diazinon, butachlor, dimethametryn and molinate in Selenastrum capriconurtum, Scenedesmus subspicatus, Chlorella vufgaris and Nitzschia palea during an exposure period of 72 hours. The study was carried out in according with the OECD Guidelines for Testing of Chemicals No 201 Alga, Growth Inhibition Test. The toxicological responses of isoprothiolane to S. capriconutum, S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ (Median Effective Concentration by growth rate) value, were 5.87, 9.91, $18.55 and 38.79\;mg\;L^{-1}$, respectively. Diazinone to S. capriconutum, S. subspicatus, C. vulgaris and palea expressed as in individual $ErC_{50}$ value, were 10.31, 11.44, >32 and $14.32\;mg\;L^{-1}$, respectively. Butachlor to S. capriconutum, S. subspicatus, C. vulgaris and N. plaea expressed as in individual $ErC_{50}$ value, were 0.002, 0.019, 8.67 and $4.94\;mg\;L^{-1}$, respectively. Dimethamelryn to S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ value, were 0.0071, 0.011, 0.0065 and $0.009\;mg\;L^{-1}$, respectively. Molinate to S. capriconutum, S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ value, were 0.44, 1.26, 48.84 and $28.52\;mg\;L^{-1}$, respectively. The sensitivities of five pesticides were different depending on freshwater alga in the order of S. capriconutum > S. subspicatus > C. vulgaris, N. palea. Highly significant correlation (r=0.9677) based on $ErC_{50}$ were found between S. capriconutum and S. subspicatus.
우리나라에서 사용되고 있는 벼재배용 농약 isoprothiolane, diazinon, butachlor, dimethametryn 그리고 molinate 등에 대한 녹조류 Selenastrum capricornutum, Scenedesmus subspicatus 그리고 Chlorella vulgaris와 규조류 Nitzschia palea의 생장저해시험 결과, isoprothiolane에 대한 S. capricornutum, S. subspicatus, C. vulgaris 그리고 N. palea의 72시간 $ErC_{50}$은 각각 5.87, 9.91, 18.55 그리고 $38.79\;mg\;L^{-1}$이었고, diazinon의 S. capricornutum, S. subspicatus, C. vulgaris 그리고 N. palea에 대하여 72시간 $ErC_{50}$은 각각 10.31, 11.44, >32 그리고 $14.32\;mg\;L^{-1}$이었다. Butachlor에 대한 S. capricornutum, S. subspicatus, C. vulgaris, 그리고 N. palea의 72시간 $ErC_{50}$은 각각 0.002, 0.019, 8.67, 그리고 $4.94\;mg\;L^{-1}$이었고, dimethametryn의 S. capricornutum, S. subspicatus, C. vulgaris 그리고 N. palea에 대한 72시간 $ErC_{50}$은 각각 0.0071, 0.011, 0.0065 그리고 $0.009\;mg\;L^{-1}$이었다. 또한 Molinate에 대한 S. capricornutum, S. subspicatus, C. vulgaris 그리고 N. palea의 72시간 $ErC_{50}$은 각각 0.44, 1.26, 48.84 그리고 $28.52\;mg\;L^{-1}$이었다. 5종 농약에 대한 4종 조류의 감수성 차이는 S. capriconutum > S. subspicatus > C. vulgaris, N. palea 순이었고, 녹조류 S. capricornutum와 S. subspicatus는 높은 감수성 상관관계(r=0.9677)를 보였다.