• Journal of Pharmaceutical Investigation
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• v.40 no.2
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• pp.101-107
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• 2010

A rapid and sensitive reversed-phase high performance liquid chromatography (HPLC) method was developed for the determination of N-(-4-Chlorophenyl)-6-hydroxy-7-methoxy-2-chromanecarboxamide (KAL-1120), a novel anti-inflammation agent, in the rat plasma. The method was applied to analyze the compound in the biological fluids such as bile, urine and tissue homogenates. After liquid-liquid extraction, the compound was analyzed on an HPLC system with ultraviolet detection at 275 nm. HPLC was carried out using reversed-phase isocratic elution with a $C_{18}$ column, a mobile phase of a mixture of acetonitril (40 v/v%) at a flow rate of 1.0 mL/min. The chromatograms showed good resolution and sensitivity and no interference of plasma. The calibration curve for the drug in plasma was linear over the concentration range of 0.05-50 ${\mu}g$/mL. The intra- and inter-day assay accuracies of this method ranged from 0.06% to 9.33% of normal values and the precision did not exceed 6.28% of relative standard deviation. The plasma concentration of KAL-1120 decreased to below the quantifiable limit at 1.5 hr after the i.v. bolus administration of 2-10 mg/kg to rats ($t_{1/2,({\alpha})}$ and $t_{1/2,({\beta})$ of 2.15 and 26.7 min at a dose of 2 mg/kg, 3.91 and 33.0 min at a dose of 10 mg/kg, respectively). The steady-state volume of distribution ($V_{dss}$) and the total body clearance ($CL_t$) were not significantly altered in rats given doses from 2 to 10 mg/kg. Of the various tissues tested, KAL-1120 was mainly distributed in the lung and heart after i.v. bolus administration. KAL-1120 was detected in the bile by 30 min after its i.v. bolus administration. However, the concentration in the urine after i.v. bolus administration became too low to measure, suggesting that KAL-1120 is mostly excreted in the bile. In conclusion, this analytical method was suitable for the preclinical pharmacokinetic studies of KAL-1120 in rats.

• Korean Journal of Environmental Agriculture
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• v.36 no.1
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• pp.63-66
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• 2017

BACKGROUND: The phenol concentrations in water samples were determined using gas chromatography after derivatization of the analyte to phenyl acetate followed by extraction using a large volume of solvent. However, this procedure requires an additional purification step and is not analytically efficient. METHODS AND RESULTS: In this study, phenol was first extracted from an acidified water sample using ethyl acetate and then acetylated using acetic anhydride in the presence of a small amount of water and $K_2CO_3$. The derivative was extracted using 1mL of n-butyl acetate. One microliter of the extract was analyzed by GC-MS without further purification. The calibration curve showed good linearity with the $r^2$ value of 0.9968. The method detection limit and the limit of quantitation were estimated to be $0.18{\mu}g/L$ and $0.56{\mu}g/L$, respectively. Repeatability (RSD, n=3) and recovery (n=3) were 9.1%-4.3% and 90.6%-110.5%, respectively. The concentrations of phenol in a few samples of stream water were distributed in the range of $2.51-7.51{\mu}g/L$. CONCLUSION: This method is simpler and faster to implement than those currently utilized and shows high analytical reliability. It can be applied to the quantitative determination of phenol concentrations in surface water and groundwater samples.

• Journal of the Korean Chemical Society
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• v.35 no.4
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• pp.389-396
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• 1991

The pre-concentration and determination of ultratrace arsenic in water samples was studied by the precipitate flotation technique. The arsenic in 1.0l of water sample, in which all suspended materials were filtered out, was coprecipitated together with La(OH)$_3$ precipitates at pH 8.5${\pm}$0.1. After the precipitate was made to be hydrophobic by adding mixed surfactant of 1 : 8 mole ratio of sodium oleate and sodium dodecyl sulfate, it was floated with the aid of tiny bubbles of nitrogen gas in a flotation cell. The floated precipitate was quantitatively collected on a micropore glass filter by the suction, dissolved with small volume of 1.0M sulfuric acid, and accurately diluted to 25.00ml with a de-ionized water. Total arsenic was spectrophotometrically determinated by forming silver diethyldithiocarbamate complex of arsine generated from arsenic in the concentrated solution. The calibration curve was linear up to 20ng/ml in the original solution. Analytical results showed that contents of arsenic in a campus wastewater and a river water were 8.2ng/ml and l.0ng/ml, respectively, and their recoveries were 93${\%}$ and 90${\%}$ in water samples which a given amount of arsenic was added into. From above result, it could be concluded that this method was applicable to the determination of arsenic in various kinds of water at low ng/ml levels.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.1
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• pp.52.2-52.2
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• 2014

The Immersion Grating Infrared Spectrometer (IGRINS) is an unprecedentedly minimized infrared cross-dispersed echelle spectrograph with a high-resolution and high-sensitivity optical performance. A silicon immersion grating features the instrument for the first time in this field. IGRINS will cover the entire portion of the wavelength range between 1.45 and $2.45{\mu}m$ accessible from the ground in a single exposure with spectral resolution of 40,000. Individual volume phase holographic (VPH) gratings serve as cross-dispersing elements for separate spectrograph arms covering the H and K bands. On the 2.7m Harlan J. Smith telescope at the McDonald Observatory, the slit size is $1^{\prime\prime}{\times}15^{\prime\prime}$. IGRINS has a $0.27^{\prime\prime}$ pixel-1 plate scale on a $2048{\times}2048$ pixel Teledyne Scientific & Imaging HAWAII-2RG detector with SIDECAR ASIC cryogenic controller. The instrument includes four subsystems; a calibration unit, an input relay optics module, a slit-viewing camera, and nearly identical H and K spectrograph modules. The use of a silicon immersion grating and a compact white pupil design allows the spectrograph collimated beam size to be 25mm, which permits the entire cryogenic system to be contained in a moderately sized rectangular vacuum chamber. The fabrication and assembly of the optical and mechanical hardware components were completed in 2013. In this presentation, we describe the major design characteristics of the instrument and the early performance estimated from the first light commissioning at the McDonald Observatory.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.14 no.2
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• pp.136-150
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• 2002

To improve the water quality of the recently constructed Siwhaho, sluice gates were operated to allow free exchange of water with the sea. This estuarine lake connected to the outer sea through narrow gates is affected mainly by flushing by gate operation and river flows and wind forcing sometimes. As a predicting tool far the water qualities, a three-dimensional finite volume model CE-QUAL-ICM is incorporated into a finite element hydrodynamic model, TIDE3D. In coupling these two different modules, a new error minimization technique is applied by considering conservation of mass. Model tests for one year after calibration and validation using field observation show that eutrophication and other biological changes reach quasi-steady state after initial 60 days of simulation, thus it would be necessary to consider moderate ramp up option to remove initial uncertainties due to cold start option. Sediment-water interaction might not be a concern in the long-term simulation, since its effect is negligible. Simulated results show the newly applied scheme can be applied with satisfaction not only fur lessening of eutrophic processes in an estuarine lake but also looking for some active circulation to improve water quality.

• Journal of Korea Water Resources Association
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• v.36 no.2
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• pp.285-300
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• 2003

An event-based, kinematic, infiltration-excess, and distributed rainfall-runoff model using weather radar and Geographic Information System(GIS) was developed to acknowledge and account lot the spatial variability and uncertainty of several parameters relevant to storm surface runoff and surface flow The developed model is compatible with raster GIS and spatially and temporally varied rainfall data. To calibrate the model, Monte Carlo simulation and a likelihood measure are utilized; allowing for a range of possible system responses from the calibrated model. Using rain gauge adjusted radar-rainfall estimates, the developed model was applied and evaluated to a limited number of historical events for the Ralston Creek and Goldsmith Gulch basins within the Denver Urban Drainage and Flood Control District (UDFCD) that contain mixed land use classifications. While based on a limited number of Monte Carlo simulations and considered flood events, Nash and Sutcliffe efficiency score ranges of -0.19∼0.95 / -0.75∼0.81 were obtained from the calibrated models for the Ralston Creek and Goldsmith Gulch basins, based on a comparison of observed and simulated hydrographs. For the Ralston Creek and Goldsmith Gulch basins, Nash and Sutcliffe efficiency scores of 0.88/0.10, 0.14/0.71, and 0.99/0.95 for runoff volume, peak discharge, and time to peak, respectively, were obtained from the model.

• Analytical Science and Technology
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• v.15 no.6
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• pp.514-520
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• 2002

The electrothermal vaporization (ETV) hollow cathode glow discharge atomic emission spectrometer for analysis of liquid sample has been developed and characterized. This system has improved the sample introduction method of electrothermal vaporization and the hollow cathode glow discharge. The sample introduction method was possible to provide high analyte transport efficiency to the plasma by helix coil made of tungsten material. In addition, small volume samples (<$30{\mu}{\ell}$) could be used. The system has glow discharge cell with special design for improvement of precision. The effect of discharge parameters such as discharge power, gas flow rate has been studied to find optimum condition. The emitted light was effectively carried into detector by fiber optic cable in UV region. The calibration curve of Pb, Cd were obtained with 3 samples.

• Journal of Digital Convergence
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• v.17 no.10
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• pp.373-379
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• 2019

Polymethyl methacrylate (PMMA), a self-polymerizing resin for removable orthodontic devices, has been used as a dental orthodontic device for many years because of its advantages such as color stability, volume stability, and tissue compatibility. However, such a removable orthodontic device has a disadvantage that the longer the use in the oral cavity due to the low strength of the PMMA fracture of the orthodontic device resin in use. In this study, zinc nanoparticles (ZNP) were mixed with orthodontic PMMA to introduce strength effect. Rectangular samples ($1.4{\times}3.0{\times}19.0mm$) of orthodontic PMMA (0, 0.5, 1.0, 2.0 and 4.0%) containing ZNP were prepared. The finished specimen was tested for three-point bending strength at a speed of 1 mm / min, and the Vickers hardness was measured three times using a hardness tester. The surface roughness was measured with a surface roughness. As a result, the 3-point bending strength did not change significantly (p>0.05). Surface energy increased significantly. As a result, we successfully synthesized ZNP in this study and prepared the dispersed resin specimen for calibration. It will be possible to develop high-density dental orthodontic resins.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.2
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• pp.404-411
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• 2019

In this study, the HEC-HMS was applied to determine rainfall-runoff processes for the Gokgyuchun basin. Several sub-basins have large-scale reservoirs for agricultural needs and they store large amounts of initial runoff. Three infiltration methods were implemented to reflect the effect of initial loss by reservoirs: 'SCS-CN'(Scheme I), 'SCS-CN' with simple surface method(Scheme II), and 'Initial and Constant rate'(Scheme III). Modeling processes include incorporating three different methods for loss due to infiltration, Clark's UH model for transformation, exponential recession model for baseflow, and Muskingum model for channel routing. The parameters were calibrated using an optimization technique with trial and error method. Performance measures, such as NSE, RAR, and PBIAS, were adopted to aid in the calibration processes. The model performance for those methods was evaluated at Gangcheong station, which is the outlet of study site. Good accuracy in predicting runoff volume and peak flow, and peak time was obtained using the Scheme II and III, considering the initial loss, whereas Scheme I showed low reliability for storms. Scheme III did not show good matches between observed and simulated values for storms with multi peaks. Conclusively, Scheme II provided better results for both single and multi-peak storms. The results of this study can provide a useful tool for decision makers to determine master plans for regional flood control management.

• Journal of the Korean GEO-environmental Society
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• v.22 no.3
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• pp.15-29
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• 2021

The purpose of this study is to establish an ecosystem model that can predict ecosystem fluctuations in the Nakdong estuary, and use this model to calculate total primary production and respiration. AQUATOX model was used as the ecosystem model, and the model was calibrated and verified using the measured data. For the calibration of the model, chlorophyll-a data measured at the Nakdong estuary were used, and the model verification was performed using DO, TN, and TP data. In general, the total primary production and respiration volume vary greatly depending on the season, but the total primary production and respiration in the Nakdong estuary were greatly influenced by the amount of water discharged from Nakdong estuary bank. When the amount of effluent increased, photosynthesis could not be performed due to the loss of phytoplankton living in the lower area, and the total primary production amounted to zero, whereas the respiration increased sharply due to the inflow of organic substances contained in the effluent. The increase in the inflow water means the inflow of organic substances contained in the inflow water, and the organic substances are decomposed by oxidation, reducing dissolved oxygen. Compared with other countries' estuaries, the Nakdong estuary shows the lowest total primary production and because the respiration is larger than the total primary production, the dissolved oxygen is depleted by the oxidation of organic matter.