• Tribology and Lubricants
• /
• v.39 no.2
• /
• pp.61-71
• /
• 2023

This study proposes a procedure for designing a labyrinth seal that meets both leakage flow rate and rotordynamic performance criteria (effective damping, amplification factor, separation margin, logarithmic decrement, and vibration amplitude). The seal is modeled using a one control volume (1CV) bulk flow approach to predict the leakage flow rate and rotordynamic coefficients. The rotating shaft is modeled with the finite element (FE) method and is assumed to be supported by two linearized bearings. Geometry, material and operating conditions of the rotating shaft, and the supporting characteristics of the bearings were fixed. A single labyrinth seal is placed at the center of the rotor, and the linearized dynamic coefficients predicted by the seal numerical model are inserted as linear springs and dampers at the seal position. Seal designs that satisfy both leakage and rotordynamic performance are searched by modifying five seal design parameters using the multi-grid method. The five design parameters include pre-swirl ratio, number of teeth, tooth pitch, tooth height and tooth tip width. In total, 12500 seal models are examined and the optimal seal design is selected. Finally, normalization was performed to select the optimal labyrinth seal designs that satisfy the system performance requirements.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.13 no.8
• /
• pp.3792-3799
• /
• 2012

The dedusting characteristics of pulse air jet type dedusting system which is widely applied in the industries were identified by utilizing the computational fluid dynamics (CFD) and the dedusting performance in modified shape of dedusting unit was compared in this study. The review on the dedusting air volume, air stream distribution and inflow velocity distribution on each shape of dedusting part showed that the case of installing the nozzle on the blow tube (Case-3) and the case of installing the double intaking tube to the venturi (Case-4 and Case-5) were more excellent than the structure (Case-1) which is widely applied in the field in its amplification effect on the air volume and extension of stream width. The specification of venturi was decided to apply the selected Case-5 for the option of the commercial back filter. It is considered that the dedusting air volume will be maintained in maximum in the case of 50 mm and 90 mm for the diameter of internal and external intaking pipe respectively.

• Korean Journal of Ecology and Environment
• /
• v.56 no.1
• /
• pp.94-103
• /
• 2023

Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria's early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

• The Korean Journal of Mycology
• /
• v.23 no.3 s.74
• /
• pp.202-208
• /
• 1995

This study describes the effects of several components on PCR amplification used for RAPD. We used different concentrations of reaction components to obtaine discrete and reproducible PCR products from Pleurotus cornucopiae. The optimum concentrations of reaction components were found to be 80 ng of template DNA, 30 pmole of 10-mer primer, $200\;{\mu}M$ dNTP, 2mM $MgCl_2$, 50 mM KCl, 10 mM Tris-HCl(pH 9.0), 0.1% Triton X-100, 1.5 unit of Taq DNA polymerase (promega) in $50\;{\mu}l$ reaction volume. The optimum annealing temperature was $35^{\circ}C$. These results proved to be valuable for characterization of Pleurotus spp.

• Proceedings of the KIEE Conference
• /
• 2002.07c
• /
• pp.1668-1670
• /
• 2002

Since more than two decades, the conventional PD detecting systems have been employed in order to detect the partial discharges occurring inside the HV power apparatus for their diagnosis by use of different type of detection such as acoustic and UHF detection method. Regardless of their wide on-site application, a certain number of technical inconveniences have been disclosed as follows : multistage amplification. large volume, susceptible to external noise and high price. In this respect, the optical measurement techniques are widely proposed in these days in this concerned field ascribed to the following advantages : immune to external EMI noise and broad band response of the Pockels cell covering from DC to GHz. However, the reliability of several proposed techniques enabling to measure the electric field inside the large high power apparatus has not yet been well approved In this work, an optical measuring system, based on the Pockels effect, has been developed for measuring the field variation due to the corona discharges occurring in air and in oil. This system consists of He-Ne laser, single mode optical fiber, multi mode optical fiber, polarizing film, Y-cut LiNbO3 cell, photo detector, digital oscilloscope and personal computer with GPIB. For this purpose, optical probe has been specially designed and realized and put into the needle-plane electrode. Afterward, same measurement is carried out in oil. We demonstrate the characteristic of the optical measuring system and the measurement results.

• Composites Research
• /
• v.36 no.3
• /
• pp.154-161
• /
• 2023

This study explored the feasibility of replacing a metal link with a carbon fiber/epoxy composite link and assessed its capacity to withstand a given load condition using failure criteria. The micromechanics of failure (MMF) criterion was employed to predict the failure mode of the composite material, and mechanical tests were conducted to obtain reference strength parameters for MMF. The findings revealed that the stress distribution was concentrated near the hole, and weaknesses were found around the hole and at the end of the link under bending conditions. Based on the failure index, matrix tensile failure was predicted at the end of the link, and fiber compression failure occurred near the hole. The methods and results obtained from this study can provide valuable guidelines for assessing the safety of composite materials under specific load conditions when replacing metal parts with carbon fiber/epoxy composites to achieve weight reduction.

• Horticultural Science & Technology
• /
• v.16 no.1
• /
• pp.21-24
• /
• 1998

RAPD markers were analyzed in order to detect the genetic variation and diversity of the fifty-two melon lines. SDS extraction method produced more and purer DNA than CTAB method. RAPD reaction conditions were optimized as follows ; 10ng template DNA, 270nM primer, $200{\mu}M$ each of dATP, dCTP, dGTP and dTTP, $0.3{\mu}unit$ dynazyme and 10x buffer brought to $15{\mu}l$ final volume with distilled water. The adequate annealing temperature was $39^{\circ}C$ and forty cycles of amplification produced the best RAPD band patterns. Among a total of 123 bands from 12 random primers, 25 polymorphic bands(20%) were selected as reliable markers. The average number of polymorphic bands per primer was 2.1 among the 52 lines. Intragroup genetic relationship based on the marker difference was closer than intergroup genetic relationship. The 52 lines could be grouped into two major group (Korean landraces and melon lines) and then melon group subdivided into two subgroups (net melon lines and no-net melon). This result corresponded to morphological grouping. Eight RAPD markers separated the Korean landraces and melon groups and four RAPD markers separated net melon and no-net melon groups.

• Journal of Plant Biotechnology
• /
• v.42 no.2
• /
• pp.117-122
• /
• 2015

Canola is a crop globally used for production of oil and biofuel. Cultivation area and import volume of living modified (LM) canola have been increasing every year. As canola import dependence has reached 100% in Korea, efforts have been made for safety management of LM canola and ecological risk assessment. We developed a set of multiplex PCR method for simultaneous detection of 5 LM canola events (Topas 19/2, Rf3, Ms8, RT73 and T45) approved in Korea. The multiplex PCR assay developed allows amplification of estimated products of 5 LM canolas from event specific primer sets. Primer extension time was skipped for a time-consuming process and two annealing steps (20 cycles at $55^{\circ}C$ and 20 cycles at $60^{\circ}C$) were performed for yielding the best result which was sufficient to distinguish five LM canolas. Our results suggest that multiplex PCR method provides a cost and time-effective approach for LM canola detection.

• Journal of Food Hygiene and Safety
• /
• v.13 no.4
• /
• pp.388-393
• /
• 1998

Recently there has been an increasing amount of foreign livestock products distributed in the domestic market due to the market opening. Some vicious dealers sell the foreign beef in the trade name of the native beef during the final distribution step to arouse the social criticism frequently. In this report, we investigated a method to distinguish the native beef from the foreign one scientifically using the PCR-RAPD, a recent gene technique. Hygienical safety was also examined using a microbiological test for toxicity of Escherichia coli 0157:H7 and the food poisoning bacteria. The conditions of DNA amplification for the PCR analysis were $1{\times}Taq$ polymerase buffer, 1.5 mM $MgCl_2,\;50\;\mu\textrm{M}$ dNTP, 100 ng primers, 2.5 unit Taq polymerase and 5~20 ng template DNA, with the fmal volume of $50\;\mu\textrm{\ell}$. The size of the amplified product was detected mostly in the range of 0.5~2.0 kbp. The size of DNA, gene marking factor, which could be a criterion distinguishing the native beef from the foreign one, appeared approximately 1.2 kbp. The native beef was distinguished from the foreign beef with more than 90% of confidence by the gene marking factor. This method was expected to be useful in the breed discrimination between the native beef and the foreign one. The hygienical test results showed that, fortunately, neither Salmonella spp. and Listeria monocytogenes which form a principal cause of the food poisoning nor Enterohemorrhagic Escherichia coli : EHEC which have provoked a recent social disturbance, were detected at all.

• Korean Journal of Microbiology
• /
• v.43 no.2
• /
• pp.91-99
• /
• 2007

For the detection of Human Immunodeficiency Virus (HIV), multiple and ultra-rapid real-time PCR methods were developed. The target DNA sequences were deduced from HIV-1 specific 495bp partial env gene (gi_1184090) and from HIV-2 specific 294 bp partial env gene (gi_1332355), and were synthesized by using PCR-based gene synthesis on the reason of safety. Ultra-rapid real-time PCR was performed by $Genspector^{TM}$ using microchip-based, $1\;{\mu}l$ of reaction volume with extremely short time in each 3 step in PCR. The detection including DNA-amplification and melting temperature analysis was completed inner 15 minutes. The HIV-1 specific 117 bp-long and HIV-2 specific 119 bp-long PCR products were successfully amplified from minimum of template,2.3 molecules of each env gene. This kind of real-time PCR was designated as ultra-rapid real-time PCR in this study and it could be applied not only an alternative detection method against HIV, but also other pathogens using PCR-based detection.