• The Korean Journal of Physiology and Pharmacology
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• 제12권6호
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• pp.323-330
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• 2008

The properties of voltage dependent $Ca^{2+}$ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times $min^{-1}$ respectively, in the presence of $1{\mu}M$ nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under $K^+$ -rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With $Cs^+$-rich pipette solution at $V_h=-80\;mV$, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine ($1{\mu}M$) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent $Ca^{2+}$ currents ($VDCC_L$).

• Journal of Power Electronics
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• 제16권4호
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• pp.1316-1323
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• 2016

Multilevel inverters (MLIs) have been preferred over conventional two-level inverters due to their inherent properties such as reduced harmonic distortion, lower electromagnetic interference, minimal common mode voltage, ability to synthesize medium/high voltage from low voltage sources, etc. On the other hand, they suffer from an increased number of switching devices, complex gate pulse generation, etc. This paper develops an ingenious symmetrical MLI topology, which consumes lesser component count. The proposed level dependent sources concoction multilevel inverter (LDSCMLI) is basically a multilevel dc link MLI (MLDCMLI), which first synthesizes a stepped dc link voltage using a sources concoction module and then realizes the ac waveform through a conventional H-bridge. Seven level and eleven level versions of the proposed topology are simulated in MATLAB r2010b and prototypes are constructed to validate the performance. The proposed topology requires lesser components compared to recent component reduced MLI topologies and the classical topologies. In addition, it requires fewer carrier signals and gate driver circuits.

• Journal of information and communication convergence engineering
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• 제9권3호
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• pp.310-314
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• 2011

This paper has presented doping profile dependent threshold voltage for DGMOSFET using analytical transport model based on Gaussian function. Two dimensional analytical transport model has been derived from Poisson's equation for symmetrical Double Gate MOSFETs(DGMOSFETs). Threshold voltage roll-off is very important short channel effects(SCEs) for nano structures since it determines turn on/off of MOSFETs. Threshold voltage has to be constant with decrease of channel length, but it shows roll-off due to SCEs. This analytical transport model is used to obtain the dependence of threshold voltage on channel doping profile for DGMOSFET profiles. Also we have analyzed threshold voltage for structure of channel such as channel length and gate oxide thickness.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1994년도 춘계학술대회
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• pp.142-145
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• 1994

Calcium(Ca) ion plays an important role to trigger the secretion of important neurotransmitters. Since Ca ion flows into the cell thru the ion selective channel, the conductance of which depends on the transmembrane potential, the voltage-dependent characteristic of Ca ion channel is crucial to elucidate the stimulus-secretion coupling of exocytosis. The present study measured the Ca ion currents thru a whole-cell configuration patch at the transmembrane potential clamped at various desired levels in the rat chromaffin cell. The resultant current-voltage relationship was differentiated to obtain dynamic conductance at each clamped voltage. Based on these measured data, five numerical parameters were extracted to reveal electrical properties of Ca ion inflow process thru the voltage-gated channel. The present study can be applied to comparing the electrical characteristics of Ca channel under different experimental conditions. Also, further study is warranted to model the conformational changes of the channel molecules.

• Restorative Dentistry and Endodontics
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• 제22권2호
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• pp.809-819
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• 1997

The purpose of this study was to develop an accurate frequency-dependent type apex locator. To compensate the impedence differences of different canal contents, voltage differences were pre-measured and stored for saline, HOsb1/$2O_2$ and NaOCl. The circuit was adjusted to recognize the different voltages of each solution, thereby the machine could automatically compensate the voltage differences. In the process of this study, the following informations were observed. 1. Most stable apical reading was obtained at 500Hz and 10kHz. 2. There were no differences in accuracy among three different frequency types, sign wave, triangular wave and rectangular wave(p>0.05). 3. Before the compensation of voltage differences, saline, H2O2 and NaOCl showed different readings at the apex even with the frequency-type (p<0.05). 4. After compensating the voltage difference, the accuracy was enhanced from + 0.42 to +0.32 in H2O2(p<0.05), from -0.34 to -0.12 in NaOCl(p<0.05).

• Biomolecules & Therapeutics
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• 제1권2호
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• pp.171-176
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• 1993

Purpose of the present study was to clarify the role of noradrenergic neural activities in hypothalamus for either triggering or maintaining hypertension in deoxycorticosterone (DOCA)-salt hypertensive rats. Two groups of animals were prepared: 1) normotensive Wistar rats and 2) DOCA-salt induced hypertensive rats. Voltage dependent $^{45}Ca^{++}$ uptake, endogenous norepinephrine release, and the catecholamine content in the hypothalamus of DOCA-salt hypertensive and normotensive Wistar rats were compared. Animals at 4, 6 and 16 week-old of two groups were sacrificed by decapitation and hypothalamus was dissected out. Voltage dependent calcium uptake and norepinephrine release were determined from hypothalamic synaptosomes either in low potassium or high potassium stimulatory condition by using $^{45}Ca^{++}$ isotope and HPLC-ECD technique. Degrees of voltage dependent $^{45}Ca^{++}$ uptake and norepinephrine release in hypothalamic synaptosomes of 16-week-old DOCA-salt hypertensive rats were significantly greater than those of age matched normotensive control rats. The norepinephrine and dopamine contents of hypothalamus were about the same in two groups of animals. These results suggest that the alteration of evoked norepinephrine release related to calcium uptake in hypothalamus may play a role in the maintenance of hypertension in DOCA-salt hypertensive rats.

• E2M - 전기 전자와 첨단 소재
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• 제9권6호
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• pp.558-563
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• 1996

We fabricated high voltage hydrogenerated amorphous silicon thin film transistors (a Si:H HVTFT) and investigated its temperature dependent characteristics of from 303 K to 363 K. The results show that the drain current was decreased at low gate voltage and increased at high gate voltage exponentially. According to the increasing the thickness of a Si layer, drain current increased. Difference of drain current at 363 K was increasd at the lower gate voltage and decreased at the higher gate voltage. When the drain and gate voltage of 100 V applied, the drain current increased linearly with rise temperature.

• Journal of information and communication convergence engineering
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• 제16권1호
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• pp.43-47
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• 2018

The existing modeling of avalanche dominated breakdown in double gate MOSFETs (DGMOSFETs) is not relevant for 10 nm gate lengths, because the avalanche mechanism does not occur when the channel length approaches the carrier scattering length. This paper focuses on the punch through mechanism to analyze the breakdown characteristics in 10 nm DGMOSFETs. The analysis is based on an analytical model for the thermionic-emission and tunneling currents, which is based on two-dimensional distributions of the electric potential, obtained from the Poisson equation, and the Wentzel-Kramers-Brillouin (WKB) approximation for the tunneling probability. The analysis shows that corresponding flat-band-voltage for fixed threshold voltage has a significant impact on the breakdown voltage. To investigate ambiguousness of number of dopants in channel, we compared breakdown voltages of high doping and undoped DGMOSFET and show undoped DGMOSFET is more realistic due to simple flat-band-voltage shift. Given that the flat-band-voltage is a process dependent parameter, the new model can be used to quantify the impact of process-parameter fluctuations on the breakdown voltage.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1371-1379
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• 2015

The Ca_v1.2 Ca²⁺ channel is essential for cardiac and smooth muscle contractility and many physiological functions. We mutated single, double, and quadruple sites of the four potential Asn (N)-glycosylation sites in the rabbit Ca_v1.2 into Gln (Q) to explore the effects of Nglycosylation. When a single mutant (N124Q, N299Q, N1359Q, or N1410Q) or Ca_v1.2/WT was expressed in Xenopus oocytes, the biophysical properties of single mutants were not significantly different from Ca_v1.2/WT. In comparison, the double mutant N124,299Q showed a positive shift in voltage-dependent gating. Furthermore, the quadruple mutant (QM; N124,299,1359,1410Q) showed a positive shift in voltage-dependent gating as well as a reduction of current. We tagged EGFP to the QM, double mutants, and Ca_v1.2/WT to chase the mechanisms underlying the reduced currents of QM. The surface fluorescence intensity of QM was weaker than that of Ca_v1.2/WT, suggesting that the reduced current of QM arises from its lower surface expression than Ca_v1.2/WT. Tunicamycin treatment of oocytes expressing Ca_v1.2/WT mimicked the effects of the quadruple mutations. These findings suggest that Nglycosylation contributes to the surface expression and voltage-dependent gating of Ca_v1.2.

• The Korean Journal of Physiology and Pharmacology
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• 제21권4호
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• pp.415-421
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• 2017

We investigated the inhibitory effect of escitalopram, a selective serotonin reuptake inhibitor (SSRI), on voltage-dependent $K^+$ (Kv) channels in freshly separated from rabbit coronary arterial smooth muscle cells. The application of escitalopram rapidly inhibited vascular Kv channels. Kv currents were progressively inhibited by an increase in the concentrations of escitalopram, suggesting that escitalopram inhibited vascular Kv currents in a concentration-dependent manner. The $IC_{50}$ value and Hill coefficient for escitalopram-induced inhibition of Kv channels were $9.54{\pm}1.33{\mu}M$ and $0.75{\pm}0.10$, respectively. Addition of escitalopram did not alter the steady-state activation and inactivation curves, suggesting that the voltage sensors of the channels were not affected. Pretreatment with inhibitors of Kv1.5 and/or Kv2.1 did not affect the inhibitory action of escitalopram on vascular Kv channels. From these results, we concluded that escitalopram decreased the vascular Kv current in a concentration-dependent manner, independent of serotonin reuptake inhibition.