• 대한지역사회영양학회지
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• 제5권3호
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• pp.432-443
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• 2000

Smoking can increase oxidative stress and thereby change the antioxidant defense system in the body. Supplementation of antioxidant vitamins might improve antioxidant status in the body. The purpose of this study was to evaluate the effectiveness of vitamin C supplementation and smoking cessation education on changes of antioxidant status and psychosocial factors related to smoking. To obtain above purposes, we investigated the effectiveness of intervention for male adolescent smokers were evalnate by assessing changes in dietary intakes, plasma antioxidant vitamin concentration, and psychosocial factors related to smoking after program completion. Subjects, male adolescent smokers, were assigned into four groups : Control group(19 students), Educ. group(19 students), Vit. C supple. group(19 students), and Educ.+Vit. C suppl. group(19 students). The Educ. group and Educ.+Vit. C suppl. group received nutrition and smoking cessation education once a week for 5 weeks. Vit. C suppl. group and Educ.+Vit. C suppl. group received 500 mg per day of ascorbic acid for 35 days. All data were collected before and after intervention. Vit. B$_2$and Vit. C intakes of all groups were increased, but the only Ca intake was increased in the Educ. group. Plasma Vit. C concentration and Ratio(plasma Vit. C/Vit. C intakes) were increased in the Vit. C suppl. group and Educ.+Vit. C suppl. group, and the Vit. C deficiency status of these groups(Vit. C suppl. group and Educ.+Vit. C suppl. group) disappeared. Showing the effects of Vit. C supplementation, plasma $\alpha$-tocopherol was increased in the Educ. and Educ.+Vit. C suppl. group, and especially high increases were seen in the Educ.+Vit. C suppl. group. Psychosocial factors related to smoking changed after the education a little. This intervention program had an impact on nutrition intakes, plasma antioxidant vitamins, and some beliefs related to smoking in male adolescent students. Various programs of nutrition and smoking cessation education and vitamin supplementation for quitting smoking must be implemented for adolescent smokers, and further studies are needed regarding sorts and amount of antioxidant nutrients and supplementation periods.

• 생명과학회지
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• 제10권3호
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• pp.254-261
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• 2000

난소를 절제한 female rat에 Vit C. Vit E, Vit C와 Vit E를 복합적으로 투여했을 때 rat의 간 조직 의 손상 정도 및 항산화 효소 활성을 관찰하기 위해 체중 150$\pm$10g이 되는 female rat에 100mg/kg 함 량의 각 실험 물질들을 이틀에 한 번 두달 간 복강내로 투여한 뒤 간 조직 중 과산화지질량 및 항상화 효소인 SOD, catalase, 총 glutathion 량 및 Glutathion peroxidase의 활성도를 측정함으로써 항산화계의 변화 및 생리 활성 변화를 살펴 보았다. 1. 간, 신장 조직 중의 과산화지질 함량은 노화군(Ovx)이 정상 군(Sham)에 비해 각각 1.78배, 1.61배 증가하였다. 그리고 각 비타민 투여군은 난소를 절제한 군에 비해 과산화질함량이 현저히 감소하였다. 2. 혈청 중 총 cholesterol 량은 노화군(Ovx)이 정상군(Sham)에 비 해 2.57배 높게 나타났다. 각 비타민 투여군은 노화군에 비해 수치가 현저히 감소하였는데, Ovx+Vit C 군의 경우는 94%정도의 억제율을 보였다. HDL-cholesterol은 노화군(Ovx)이 정상군(Sham)에 비해 36.7% 감소하였다. 각 비타민 투여로 HDL-cholesterol 함량이 좋은 수준으로 증가되었고, Ovx+Vit Erns은 노화군보다 2.52배 높게 나타났다. 동맥경화지수도 Ovx+Vit E 군이 아주 낮게 나타났다. 3. 간 조직 중 SOD 활성도는 노화군(Ovx)이 정상군(Sham)보다 5.3배 높게 나타났고, 각 비타민 투여로 free radical 생성이 억제되어 항산화효소인 SOD 활성도를 감소시켰다. 간 조직 중 catalase 활성도는 노화군 (Ovx)이 정상군(Sham)보다 1.58배 정도 높게 나타났는데, 각 비타민 투여로 catalase 활성도가 낮아졌 고, Ovx+Vit E 군은 탁월한 감소 효과를 보였다. 4. 간 조직 중 총 glutathion의 활성도는 정상군 (Sham)이 노화군(Ovx)에 비해 1.22배 정도 높게 나타났고, 각 비타민 투여군 중 Ovx+Vit Erns의 경우 간 조직 중 총 glutathionfid이 가장 높게 나타났다. 간조직 중 reduced-glutathion (GSH)함량과 총 glutathion 량에 대한 oxidized-glutathion (GSSG)의 비율, 또한 Ovx+Vit E 군의 수치가 가장 높게 나 타났다. Vit E가 항산화에 좋은 효과가 있음을 알 수 있었다. 그러나, GPx의 활성 수치는 그 다지 유의 한 변화를 나타내지는 않았다.

• 대한지역사회영양학회지
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• 제6권3호
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• pp.282-289
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• 2001

The study evaluated the effectiveness of intervention for male adolescent smokers by making an assessment in terms of changes in food habits, nutrition knowledge, plasma catalase, superoxide dismutase(SOD), glutathione peroxidase peroxidase(GSH-px) activities and thiobarbituric acid reactive substance(TBARS) after Vit C supplementation and nutrition education. The subjects, male adolescent smokers, were assigned into four groups : Control group(19 students), education(Educ.) group(19 students), Vit C supplementation (suppl) group(19 students), and Educ. + Vit C suppl. group(19 students). The Educ. group and Educ. + Vit C suppl. group received nutrition education once a week for 2 weeks. The Vit C suppl. group Educ. + Vit C suppl. group received 500 mg ascorbic acid for 35 days. All data were collected before intervention and after intervention. Nutrition knowledge of those who received education increased, and the frequency of fruit and yellow-green vegetable consumption also increased. Plasma antioxidant enzyme activities were not different except for the SOD activity in the Educ. + Vit C suppl. group, which was significantly increased. The plasma ceruloplasmin level of groups that received Vit C supplementation was reduced more than any other groups, and the specific ceruloplasmin ferroxidase activity of groups that received Vit C supplementation was elevated more than other groups. These intervention programs had an impact on food habits, nutrition knowledge, plasma antioxidant enzyme activities, and plasma TBARS in male adolescent smokers. Various nutrition education programs must be implemented for adolescent smokers, and further studied are needed regarding sorts and amount of antioxidant nutrients and supplementation duration.

• 한국식품영양과학회지
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• 제40권12호
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• pp.1750-1756
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• 2011

방사선 조사에 따른 타락죽의 관능적 품질 저하를 개선하기 위한 연구의 일환으로 항산화제(Vit. C 및 Vit. E) 첨가 후, 실온 또는 냉동 방사선 조사와의 병용처리가 타락죽의 이화학적 및 관능적 품질에 미치는 영향을 평가하였다. 항산화제 첨가와 실온 방사선 조사 효과를 확인한 결과 타락죽의 pH는 Vit. E 첨가에 따른 변화가 없었으나, Vit. C의 경우 첨가량이 증가함에 따라 유의적으로 감소하였다(p<0.05). 지방산패도와 점도의 경우 Vit. C 및 Vit. E 첨가량이 증가할수록 방사선 조사에 의한 지방산화 및 점도의 감소가 유의적으로 억제되는 것으로 나타났다. 색은 Vit. C 및 Vit. E 첨가에 의한 변화는 없는 것으로 나타났다. 관능평가 결과 Vit. C 및 Vit. E 첨가구는 무첨가구에 비해 방사선 조사 타락죽의 품질을 유의적으로 향상시키는 것으로 나타났으며, 각각 0.1% 첨가구가 가장 좋은 것으로 나타났다. 항산화제(Vit. C 및 Vit. E 0.1%)와 냉동 방사선 조사 병용처리 효과를 확인한 결과, 항산화제와 냉동 방사선 조사 병용처리 시 방사선 조사에 의한 지방산패와 점도 감소 억제 및 관능적 품질저하의 개선에 매우 효과적인 것으로 나타났으며, 특히 Vit. C 및 Vit. E를 각각 0.1%씩 함께 첨가할 경우 효과가 가장 좋은 것으로 나타났다. 따라서 방사선 조사에 의한 타락죽의 관능적 품질 저하는 0.1%의 Vit. C 및 Vit. E를 각각 혼합 첨가와 냉동 방사선 조사의 병용처리를 통해 타락죽의 품질을 가장 효과적으로 개선시킬 수 있을 것으로 사료되었다.

• Journal of the Korean Wood Science and Technology
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• 제48권2호
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• pp.166-180
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• 2020

Rhododendron schlippenbachii have been used as a medicine because of their various biological activities. In this study, R. schlippenbachii ethanol extract was evaluated for the treatment of vitiligo. The R. schlippenbachii ethanol extract did not show any cell cytotoxicity. The effect on mushroom tyrosinase and cellular tyrosinase activities were further assessed. In addition, the determination of melanin content in melanocytes was measured using both the B16 melanoma cells and C57BL/6J Ler-vit/vit mice. Finally, the existence of quercetin in R. schlippenbachii was confirmed by qualitative analysis using HPLC. The results clearly demonstrated the R. schlippenbachii extract enhanced melanogenesis and also increased tyrosinase activity in cultured melanoma cells and C57BL/6J Ler-vit/vit mice. In addition, treatment with R. schlippenbachii extract led to a higher content of melanin and eumelanin in C57BL/6J Ler-vit/vit mice hair than in control (untreated) mice, which demonstrated the therapeutic effect of hair-graying associated with vitiligo. Finally, we confirmed a notable increase in melanocytes in the skin of C57BL/6J Ler-vit/vit mice treated with R. schlippenbachii extract compared with the control. Extracts of R. schlippenbachii was shown to be potent tyrosinase and melanin synthesis activator in B16 melanoma cells. The R. schlippenbachii extract have significantly higher melanin content than the untreated control in C57BL/6J Ler-vit/vit mice hair. The results suggest that R. schlippenbachii extract might be considered as an alternative treatment for improvement of vitiligo.

• 한국축산식품학회지
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• 제22권2호
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• pp.108-114
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• 2002

Vitamin E와 selenium의 급여가 거세한우육의 진열저장중 (5$^{\circ}C$, 1,200 lux) 육색안정성에 미치는 영향을 조사하였다. 실험처리구는 대조구(Vit E 27 IU/head/day, Se 0.09 mg/head/day), Vitamin E 급여구(2,500 IU/head/day), Selenium 급여구(20 mg/head/day), Vitamin E와 Selenium 복합 급여구 (Vit E 2,500 IU/head/day, Se 20 mg/head/day)로 나누어 실험하였다. CIE a*값, chroma값, oxymyoglobin함량(%) 및 R630-R580간은 모든 처리구에서 저장기간이 길어질수록 유의적으로 감소하는 경향을 나타내었으며(p<0.05), 특히 대조구가 저장기간이 길어질수록 현저한 감소현상을 보였다. Metmyoglobin(%)의 함량은 저장 전(0일)에는 처리구간에 유의적인 차이를 나타내지 않았으나(p>0.05) 저장기간이 경과 함으로써 대조구의 metmyoglobin 형성율이 현저하게 증가하여 다른 처리구에 비해 변색이 가속화됨을 알 수 있었다. 지질의 산패 정도를 나타내는 TBARS는 Se구와 Vit E+Se구가 대조구와 Vit E구보다 유의적으로 낮은 값을 나타내었다. 환원력은 저장 전에는 대조구가 유의적으로 낮게 나타났으며 3일 저장시 대조구가 다른 처리구에 비해 현저히 낮아짐을 알 수 있었다. 결과적으로 selenium 급여구들(Se구, Vit E+Se 구)의 경우 대조구와 vitamin E급여구에 비해 지질산화에 대해 안정성을 가졌으며, 육색이나 육색소에 있어서는 Vit E구, Se구, Vit E+Se구가 대조구보다 저장안정성을 보였으나 이 3가지 처리구간에는 유의적인 차이가 나타나지 않았다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.282-282
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• 2004

동물에서 활성산소계(reactive oxygen species, ROS)의 지속적이고 과도한 노출과 산화스트레스는 생리적 기능의 저하, 노화 또는 많은 질환의 원인이 되고 있으며 항산화제의 투여효과에 대한 연구가 최근 많이 이뤄지고 있다. 본 연구는 Vit. C의 공급이 절대. 필요한 guinea pig를 공시하여 녹차 추출물 중 항산화제인 polyphenol과 Vit. C의 경구 투여에 따른 정소 내 정자형성과 항산화기능의 변화 및 액상 돼지정액에 첨가 후 정액성상 변화에 대하여 조사하였다. (중략)

• 대한본초학회지
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• 제31권4호
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• pp.35-41
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• 2016

Objective : The purpose of this study is to investigate the anti-oxidative effect of Jeokbaekhaogwanjoong-tang (JGT) distillate in spleen cells of aged rats.Methods : This experiment was performed using spleen cells of 10w, 52w, 72w old sprague dawley (SD) rats. The spleen cells of 10, 52, 72 weeks old rats were divided into three groups; DW group, Vit. C group, and JGT group. The cells of DW group were treated with distilled water. The cells of Vit.C group were treated with Vitamin C. And the cells of JGT group were treated with JGT distillate. After the treatment of DW, Vit.C, and JGT, the levels of superoxide dismutase (SOD), glutathione (GSH), nitric oxide (NO) and lipid peroxidation (MDA) were measured.Results : In the spleen cells of 72 weeks old rats, SOD activity was significantly increased in the JGT group compared to those in the DW group and the Vit.C group. In the spleen cells of 52 weeks old rats, GSH activity was significantly increased in the JGT group compared to the Vit.C group, and NO concentration was significantly decreased in the JGT group compared to the DW group and the Vit.C group. MDA concentration was not changed significantly in any cell groups by the treatment of JGT distillate.Conclusions : Jeokbaekhaogwanjoong-tang (JGT) distillate may have an anti-oxidative effects in the spleen cells of aged rats. Further investigation is needed for the anti-aging effect of JGT.

• 한국조리학회지
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• 제8권2호
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• pp.217-226
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• 2002

Stuffed pork cutlets were prepared by adding kimchi, pineapple, and seasoned green onion, and they were analyzed to measure sensory and nutritional characteristics. Sensory evaluation means of stuffed pork cutlet with kimchi, pineapple and seasoned small green onion was significantly higher than means of control pork cutlet especially for appearance after cutting, tenderness of meat, tenderness of stuffing, moistureless of stuffing, balance of meat and stuffing, flavor before taste, and flavor after taste. Dietary fiber, Vit. A, and Vit. C of kimchi pork cutlet was higher than them of control pork cutlet and frozen pork cutlet Dietary fiber, K and Vit. C of pineapple pork cutlet was higher than them of control pork cutlet. And Dietary fiber, Vit Ca, and P contents of pineapple pork cutlet was higher than them of frozen pork cutlet. Vit. A, $\beta$-carotene, dietary fiber, Ca, and P contents of seasoned small green onion pork cutlet was higher than them of control pork cutlet and frozen cutlet.

• 한국식품영양학회지
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• 제34권4호
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• pp.390-397
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• 2021

The purpose of this study was to investigate at how the quality of citron changed during storage as a result of the browning inhibitor treatment. In the browning inhibitor treatment, Vit.C, Vit.C+NaCl, Vit.C+NaCl+CD substances were used. As a result of investigating the browning degree, Vit.C+NaCl+CD showed the lowest value of 0.76 when stored for 12 weeks. The 𝚫E of the chromaticity value indicated that significant color change occurred when the value was high. As the Vit.C+NaCl+CD mixture showed the lowest value of 46.01 at 25℃, it was found that browning did not occur much compared to other treatments. The change in polyphenol oxidase (PPO) activity of citron increased as browning progressed. Among the browning inhibitor solutions, Vit.C+NaCl+CD solution showed the lowest value 118.8 u/g at 25℃ after 12 weeks. Based on these findings, it seems that CD mixing solution can be used as a citron browning inhibitor.