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Studies on the Maintenance of Bioluminescence from Stored Photobacterium phosphoreum (Photobacterium phosphoreum의 생체발광 유지도에 관한 연구)

  • 김현숙;정성제;전억한
    • Microbiology and Biotechnology Letters
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    • v.28 no.2
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    • pp.117-123
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    • 2000
  • The object of this work is to improve the maintenance of bioluminescence from stored Photobacterium phosphoreum in a view of developing continuous monitoring system for pollutants. The long-term experiments were performed to determine the effect of storage temperature and immobilization on the maintenance of bioluminescence and viability of P. phosphoreum. A naturally luminescent bacterium, P. phosphoreum was starved in 2.5% Nael solution at $20^{\circ}C$, $4^{\circ}C$, -$20^{\circ}C$ and -$70^{\circ}C$ for 30 days. In vivo luminescence was measured by luminometry, and total cell concentrations and concentrations of culturable and viable cells were determined by acridine orange staining, dilution plate counting, and direct viable counting, respectively. The bioluminescence emission from cells stored at 4De was maintained up to 10 days while those with starved cells at other temperature ranges decreased to background level within 3 days. In terms of viability of cells, concentrations of cells stored at $20^{\circ}C$ were rapidly decreased as a result of cell lysis, leading to a drop in culturable and viable counts while cells stored at $4^{\circ}C$ was shown viable but nonculturable state during starvation. With immobilized cells on strontium alginate, the bioluminescence showed higher maintenance than free cells and decreased with count number of nonculturable cells.

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A Study on the Payment Mechanism of Independent Guarantee -focusing on matters that the relevant parties involved should know- (청구보증상 지급메커니즘에 따른 실무상 유의점)

  • Oh, Won-Suk;Kim, Pil-Joon;Lee, Woon-Chang
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.46
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    • pp.133-158
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    • 2010
  • Independent guarantee is a creation of the need from the both sides, i.e. the applicant (principal debtor) and the beneficiary (creditor). The former used to have to deposit cash in favor of the beneficiary in case of his default, which laid a burden on his liquidity while the latter still wanted to have the equivalent to cash. Independent guarantee satisfied the both parties by freeing the applicant of a deposit and maintaining the beneficiary's right at the same time. The fact that independent guarantee has three payment mechanisms is not widely known to the public. They are (i) payment on first demand, (ii) payment upon submission of third-party documents, (iii) payment upon submission of an arbitral or court decision. From the applicant's point of view, the order in his favor is (iii), followed by (ii) and (i). As there shouldn't be a case where one party is at a disadvantage against the other, useful insight is being sought for the benefit of the applicant. First, the applicant can offer his intention to provide a payment mechanism (ii) or (iii) rather than (i) if he must deliver it. Second, if the beneficiary still wants to have (i) and the applicant is in a position not to reject it, the latter should thoroughly check any provisions that may work against him later. Third, the applicant could use counterbalancing provisions in underlying contract to cope with protective clauses in the guarantees. Forth, the applicant should review the beneficiary's sincerity to prevent unfair calling risks. The applicant may use an ECA(Export Credit Agency) in his country to which he can transfer not only unfair calling risks, but also political risks. On the other hand, a bank needs to keep the following advice in mind. The foremost important thing for the bank not to forget is that it provides a guarantee as a service provider, not as a responsible party for the feasibility of the project, etc. Credit risk of the applicant should require the greatest attention when issuing a guarantee: the bank should look into the possibility that it can procure immediate reimbursement from its customers after payment to the beneficiary. Second, the applicant's ability to complete the project should be reviewed by checking its track records, techniques and reputation, etc. Third, the bank may also use an ECA to cover the beneficiary's unfair calling risks as well as political risks. In the case of Korea, as Korea Export Insurance Corporation(KEIC) can cover all the risks mentioned above, the bank could use its service called 'Export Bond Insurance.' What's better for the bank is that ECA cover can enhance the bank's asset quality by putting it zero on its risk weighted asset.

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An Analysis of Primary Causes for Waiting for Inpatient Admission and Length of stay at Emergency Medical Center(EMC) (응급의료 센터의 체류 및 입원대기 시간 지연 요인 - 일개 의료기관을 중심으로 -)

  • Kil Suk-Yong;Kim Ok-Jun;Park Jin-Sun
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.6 no.3
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    • pp.522-531
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    • 1999
  • This research identifies the ingress to egress primary factors that causes a patient to receive delayed emergency medical care. This material was collected between February 1st to 28th, 1998. Research envolved 4,118 people who visited the college emergency medical center in Kyeongido Province, South Korea. Medical records were examined, using the retrospective method. to determine the length of stay and the main cause for waiting. Results are as follows : 1. The age group with the highest admission rate was 10 and under, approximately 1,394 (33.9%). Followed by an even distribution for ages between 11-50 at 10-15% for their respective ranges. The lowest admission rate was 50 years and above. 2. From the 4,118 records examined, 3,489 received outpatient treatment (84.7%); 601 were admitted for inpatient care (14.6%); 25 arrived dead on arrival (0.6%); and 4 people died at the hospital. 3. Between 7PM to 12AM, 42.9% were admitted to the EMC. The hours from 9PM to 11PM recorded the highest admission rate and 5AM to 8AM was the lowest From 8PM to 12AM, the most beds were occupied. 4. For most patients. the average length of stay was approximately 2.2 hours. By medical department, external medicine was the longest for 2.8 hours. Pediatrics was the shortest for 1.6 hours. The average waiting period for inpatient admission was 2.6 hours. Inpatient admission for pediatrics and external medicine was 3.4 hours and 2.2 hours respectively. 5. Theses are primary factors for delay at EMC: 1) pronged medical consultations to decide between inpatient versus outpatient treatment, and delaying to be inpatient, 2) when you call physicians they are delayed to come 3) Understaffing during peak or critical hours, 4) Excessive consulting with different medical departments, 5) some patients require longer monitoring periods, 6) medical records are delayed in transit between departments, 7) repeated laboratory tests make delay the result, 8) overcrowded emergency x-ray place causes delay taking x-ray and portable x-ray, 9) the distance between EMC and registration and cashier offices is too far. 10) hard to control patient's family members. The best way to reduce EMC waiting and staying time is by cooperation between departments, both medical and administrative. Each department must work beyond their job description or duty and help each other to provide the best medical service and satisfy the patient needs. The most important answer to shortened the EMC point from ingress to egress is to see things from a patient point of view and begin from there to find the solution.

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Chemical Changes of the Deep Fat Frying Oils Used Commercially (일반시장에서 튀김식품에 사용된 기름의 화학적 변화)

  • Joo, Kwang-Jee;Ha, Gy-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.3
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    • pp.247-254
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    • 1989
  • In deep-fat frying, the fats and oils are used over and over again, and moisture and air are mixed in to the hot oil. Many reports related to these fats and oils have been established that thermal and oxidative decomposition products and polymers formed under the conditions of deep fat frying are harmful to health. This work was carried out with 3 domestic frying oils and 6 used oils commercially, and that there were difficulties in finding a good definition between fresh oil and used oil with adding unheated oil. As starting materials, commercially used soybean oil and rapeseed oil already passed over induction period in the lipids oxidation standard. From the qualitative point of view, they were inferior to domestic frying oils. Free fatty acid and peroxide value of heated oils were increased gradually by the time pass whereas iodine value were decreased. After adding unheated oil to the heated oil, these values were restored to that of initial levels. On the other hand, content of polar components in the heated oil were directly related to the heating time. This result showed that polar compounds may be a clear indicator of used oils. Fatty acid composition in the used oils, unsaturated fatty acids such as linoleic and linolenic acid decreased while saturated fatty acid content increased with heating.

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Nonlinear Characteristic Analysis of Charging Current for Linear Type Magnetic Flux Pump Using RBFNN (RBF 뉴럴네트워크를 이용한 리니어형 초전도 전원장치의 비선형적 충전전류특성 해석)

  • Chung, Yoon-Do;Park, Ho-Sung;Kim, Hyun-Ki;Oh, Sung-Kwun
    • Journal of the Korean Institute of Intelligent Systems
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    • v.20 no.1
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    • pp.140-145
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    • 2010
  • In this work, to theoretically analyze the nonlinear charging characteristic, a Radial Basis Function Neural Network (RBFNN) is adopted. Based on the RBFNN, an charging characteristic tendency of a Linear Type Magnetic Flux Pump (LTMFP) is analyzed. In the paper, we developed the LTMFP that generates stable and controllable charging current and also experimentally investigated its charging characteristic in the cryogenic system. From these experimental results, the charging current of the LTMFP was also found to be frequency dependent with nonlinear quality due to the nonlinear magnetic behaviour of superconducting Nb foil. On the whole, in the case of essentially cryogenic experiment, since cooling costs loomed large in the cryogenic environment, it is difficult to carry out various experiments. Consequentially, in this paper, we estimated the nonlinear characteristic of charging current as well as realized the intelligent model via the design of RBFNN based on the experimental data. In this paper, we view RBF neural networks as predominantly data driven constructs whose processing is based upon an effective usage of experimental data through a prudent process of Fuzzy C-Means clustering method. Also, the receptive fields of the proposed RBF neural network are formed by the FCM clustering.

Population Characteristics Influencing Treatment Service Use among Individuals with Drug Dependency (마약류 의존자 치료재활 서비스 이용에 영향을 미치는 개인적 특성에 관한 연구)

  • Kim, Nang-hee
    • Korean Journal of Social Welfare Studies
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    • no.39
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    • pp.395-423
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    • 2008
  • This study investigated population characteristics that influencing treatment service use of people who are voluntarily using drug dependency treatment services by using logistic and hierarchical regression analysis. The research model of the current study was driven by the framework of the 'the Behavioral Model of Health Services Use(the Andersen model)' that has been broadly applied to study on health behavior. This study used data from a sample group of 80 adults by using purposive sampling. This study found that some predisposing factors, enabling factors and need factors have direct effects on service use. In detail, individuals who graduated from high school use drug dependency treatment utilities more than those who did not. Further, individuals who were given more support from family, peers, or others, use the treatment utilities more frequently and were more willing to use the utilities continuously. Furthermore, the greater the perceived need felt by the dependent, the greater the tendency to enter hospitals or shelters. The important implications of this study for social work practice and social policy can be summarized as follows: first, this study supports the idea that intervention for drug dependents in Korea should be focused on environment resources rather than population characteristics; and government must support drug dependent treatment systems; the present study was the first to investigate Korean drug dependents through taking a more positive view, as well as the first to apply 'the Behavioral Model of Health Services Use', and as such represents an example of how studies could be productively conducted in the future. Despite these implications, there remain some limitations in this study. These include the following: limitation in generalizability of the results; the cross-sectional nature of the study design; survey research through the questionnaire method; using foreign scales; and the difficulty of classifying treatment settings.

Development of MRI Simulator Early Diagnosis Program for Self Learning (자가 학습을 위한 MRI Simulator 초기 검사 프로그램 개발)

  • Jeong, Cheon-Soo;Kim, Chong-Yeal
    • The Journal of the Korea Contents Association
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    • v.15 no.9
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    • pp.403-410
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    • 2015
  • Since 1970, MRI has greatly been developing in terms of strength of magnetic field, the number of receipt channels, and short time of examination. With the development of digital systems and wireless network, hospitals have also acquired, saved, and managed digital images taken by various kinds of medical imaging equipment. However, domestic universities fail to provide practice training course independently thanks to expensive practice equipment and high maintenance cost, and rely on clinical training. Therefore, this study developed a MR patient diagnosis program based on Windows PC to help out students before their working in clinical filed. The designed Relational Database of MRI Simulator is made up of seven tables according to functions and data characteristics. Regarding the designed patient information, each stepwise function was classified by the patient registration method in clinical field. In addition, on the assumption of the basic information for diagnosis, each setting and content were classified. The menu by execution step was arrayed on the left side for easy view. For patient registration, a patient's name, gender, unique ID, birth date, weight, and other types of basic information were entered, and the patient's posture and diagnosis direction were set up. In addition, the body regions for diagnosis and Pulse Sequence were listed for selection. Also, Protocol name and other additional factors were allowed to be entered. The final window was designed to check diagnosis images, patient information, and diagnosis conditions. By learning how to enter patient information and change diagnosis conditions in this program, users will be able to understand more theories and terms learned in practice and thereby to shorten their learning time in actual clinical work.

A Study of Sowol's and Jiyoung's Sijo (소월(素月)과 지용(芝溶)의 시조(時調))

  • Lee, Tae-Hyee
    • Sijohaknonchong
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    • v.26
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    • pp.243-261
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    • 2007
  • In this study, I have looked though the Sijo made by Sowol and Jiyoung who were the frontiers of the Korean modern poetry. Through this study, I've tried to research the relationship between the free verse and their versifying Sijo. Sowol made the eight Sijo : six Sijo made in his early days. and the others made in later days. Sowol's early free verse tended to show the regular form. Although his 6 Sijo maintained the traditional form, he tried to make the formal changes. But the subject of those were in the way of view points of old Sijo. But the rest two Sijo strongly showed the problem of reality. Jiyoung made the 9 Sijo in his early days. But Jiyoung's is different from Sowol's. Jiyoung tried to make a new way of expressing the poetic images while Sowol was focused on the formal changes. Also, his final work 'Eun' had the feature of fixed form of verse. Through the research mentioned above, I confirmed that their tries to make the fixed form of verse were the background of developing and pioneering their free verse.

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Political Geography of Ulsan Oil Refinery (울산공업단지의 서막, 정유공장 건설의 정치지리)

  • Gimm, Dong-Wan;Kim, Min-Ho
    • Journal of the Korean Geographical Society
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    • v.49 no.2
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    • pp.139-159
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    • 2014
  • This study problematizes the dominance of developmental state theory and its negative influences in the field of Korean studies, in particular, dealing with the industrialization during the developmental era, 1960s~70s. As is generally known, the theory has been in a position of unchallenged authority on the industrialization experience of East Asian countries, including South Korea. However, at the same time, it has also misled us into overlooking strategic relations that had articulated the state forms at multiple scales. This study aims to reconstruct the historical contexts by the theorizing prompted by recent work on state space. I shed light on the multiscalar strategic relations that had shaped the Ulsan refinery plant as a representative state space of the South Korean industrialization during two decades after liberation. Specifically, the study illustrates the features and roles of Cold War networks and multiscalar agnets such as Nam Goong-Yeon. By identifying the plant as a result of sequential articulations between Ulsan and other scales, this study concludes by suggesting to reframing the strategic relational spaces, beyond the view of methodological nationalism, in the perspective of multiscalar approach.

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NCS-based Education & Training and Qualification Proposal for Work-Learning Parallel Companies Introducing Smart Manufacturing Technology (스마트 제조기술을 도입하는 일학습병행 학습기업을 위한 NCS 기반 교육훈련 및 자격 제안)

  • Choi, Hwan Young
    • Journal of Practical Engineering Education
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    • v.12 no.1
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    • pp.117-125
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    • 2020
  • According to the government's smart factory promotion project for small and medium-sized enterprises, more than 10,000 intelligent factories are scheduled or already built in the country and the government-led goal is to nurture 100,000 skilled workers by 2022. Smart Factory introduces numerous types of education and training courses from the supplier's point of view, such as training institutions belonging to local governments, some universities, and public organizations, in the form of an efficient resource management system and ICT technology convergence in the automated manufacturing equipment. The lack of linkage with the NCS, the standard for training, seems to have room for rethinking and direction. Results of survey is provided for the family companies of K-University in the metropolitan area and Chungnam area, and analyzes job demands by identifying whether or not they want to introduce smart factories. Defining the practitioners who will serve as a window for the introduction of smart factory technology within the company, setting up a training goal in consideration of the career path, and including the level of training required competency units, optional competency units, and training time suitable for introducing and operating smart factories. Author would like to present an NCS-based qualification design plan.