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Molecular and Functional Characterization of Monocot-specific Pex5p Splicing Variants, Using OsPex5pL and OsPex5pS from Rice (Oryza sativa)

  • Lee, Jung Ro;Jung, Ji Hyun;Kang, Jae Sook;Kim, Jong Cheol;Jung, In Jung;Seok, Min Sook;Kim, Ji Hye;Kim, Woe Yeon;Kim, Min Gab;Kim, Jae-Yean;Lim, Chae Oh;Lee, Kyun Oh;Lee, Sang Yeol
    • Molecules and Cells
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    • v.23 no.2
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    • pp.161-169
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    • 2007
  • We identified two alternatively spliced variants of the peroxisomal targeting signal 1 (PTS1) receptor protein Pex5ps in monocot (rice, wheat, and barley) but not in dicot (Arabidopsis and tobacco) plants. We characterized the molecular and functional differences between the rice (Oryza sativa) Pex5 splicing variants OsPex5pL and OsPex5pS. There is only a single-copy of OsPEX5 in the rice genome and RT-PCR analysis points to alternative splicing of the transcripts. Putative light-responsive cis-elements were identified in the 5' region flanking OsPEX5L and Northern blot analysis demonstrated that this region affected light-dependent expression of OsPEX5 transcription. Using the pex5-deficient yeast mutant Scpex5, we showed that OsPex5pL and OsPex5pS are able to restore translocation of a model PTS1 protein (GFP-SKL) into peroxisomes. OsPex5pL and OsPex5pS formed homo-complexes via specific interaction domains, and interacted with each other and OsPex14p to form hetero-complexes. Although overexpression of OsPex5pL in the Arabidopsis pex5 mutant (Atpex5) rescued the mutant phenotype, overexpression of OsPex5pS only resulted in partial recovery.

Process Fault Probability Generation via ARIMA Time Series Modeling of Etch Tool Data

  • Arshad, Muhammad Zeeshan;Nawaz, Javeria;Park, Jin-Su;Shin, Sung-Won;Hong, Sang-Jeen
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.241-241
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    • 2012
  • Semiconductor industry has been taking the advantage of improvements in process technology in order to maintain reduced device geometries and stringent performance specifications. This results in semiconductor manufacturing processes became hundreds in sequence, it is continuously expected to be increased. This may in turn reduce the yield. With a large amount of investment at stake, this motivates tighter process control and fault diagnosis. The continuous improvement in semiconductor industry demands advancements in process control and monitoring to the same degree. Any fault in the process must be detected and classified with a high degree of precision, and it is desired to be diagnosed if possible. The detected abnormality in the system is then classified to locate the source of the variation. The performance of a fault detection system is directly reflected in the yield. Therefore a highly capable fault detection system is always desirable. In this research, time series modeling of the data from an etch equipment has been investigated for the ultimate purpose of fault diagnosis. The tool data consisted of number of different parameters each being recorded at fixed time points. As the data had been collected for a number of runs, it was not synchronized due to variable delays and offsets in data acquisition system and networks. The data was then synchronized using a variant of Dynamic Time Warping (DTW) algorithm. The AutoRegressive Integrated Moving Average (ARIMA) model was then applied on the synchronized data. The ARIMA model combines both the Autoregressive model and the Moving Average model to relate the present value of the time series to its past values. As the new values of parameters are received from the equipment, the model uses them and the previous ones to provide predictions of one step ahead for each parameter. The statistical comparison of these predictions with the actual values, gives us the each parameter's probability of fault, at each time point and (once a run gets finished) for each run. This work will be extended by applying a suitable probability generating function and combining the probabilities of different parameters using Dempster-Shafer Theory (DST). DST provides a way to combine evidence that is available from different sources and gives a joint degree of belief in a hypothesis. This will give us a combined belief of fault in the process with a high precision.

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Development of a Korean Version of an Advance Directive Model via Cognitive Interview (인지면담을 이용한 한국형 사전의료의향서 모델 개발)

  • Kim, Shin Mi;Hong, Young Sun;Hong, Sun Woo;Kim, Jin Shil;Kim, Ki Sook
    • Journal of Hospice and Palliative Care
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    • v.16 no.1
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    • pp.20-32
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    • 2013
  • Purpose: The purpose of the study was to develop a feasible form of a Korean version of advance directives (K-AD). Methods: Sixteen adults participated in this study: 10 aged 20~50 years and nine aged 65 years or older. Using a draft version of the K-AD, cognitive interview was conducted on the participants to establish a culturally acceptable form of advance directives whose directions can be understood and responded accordingly by the general population. Results: Cognitive interviews revealed areas of concerns for the draft version of K-AD: lack of instructions or clarity for technical and medical terms, context complexity and inadequate response categories. The draft version was revised by rewording, offering examples and rearranging the context. Editorial style was added with appropriate uses of bold fonts, bullet-points and underlines to facilitate interviewees' cognitive responses. Conclusion: Study results feasibility of the revised version of the K-AD. Further study should be performed with a larger number of participants to develop a K-AD with an acceptable level of reliability and validity.

Detection of Precise Crop Locations under Vinyl Mulch using Non-integral Moving Average Applied to Thermal Distribution

  • Cho, Yongjin;Yun, Yeji;Lee, Kyou-Seung;Lee, Dong-Hoon
    • Journal of Biosystems Engineering
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    • v.42 no.2
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    • pp.117-125
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    • 2017
  • Purpose: Damage to pulse crops by wild birds is a serious problem. The damage is to such an extent that the rate of damage during the period between seeding and cotyledon stages reaches 54.6% on an average. In this study, a crop-position detection method was developed wherein infrared (IR) sensors were used to determine the cotyledon position under a vinyl mulch. Methods: IR sensors that helped measure the temperature were used to locate the cotyledons below the vinyl mulch. A single IR sensor module was installed at three locations of the crops (peanut, red lettuce, and crown daisy) in the cotyledon stage. The representative thermal response of a $16{\times}4$ pixel area was detected using this sensor in the case where the distance from the target was 25 cm. A spatial image was applied to the two-dimensional temperature distribution using a non-integral moving-average method. The collected data were first processed by taking the moving average via interpolation to determine the frame where the variance was the lowest for a resolution unit of 1.02 cm. Results: The temperature distribution was plotted corresponding to a distance of 10 cm between the crops. A clear leaf pattern of the crop was visually confirmed. However, the temperature distribution after the normalization was unclear. The image conversion and frequency-conversion graphs were obtained based on the moving average by averaging the points corresponding to a frequency of 40 Hz for 8 pixels. The most optimized resolutions at locations 1, 2, and 3 were found on 3.4, 4.1, and 5.6 Pixels, respectively. Conclusions: In this study, to solve the problem of damage caused by birds to crops in the cotyledon stage after seeding, the vinyl mulch is punched after seeding. The crops in the cotyledon stage could be accurately located using the proposed method. By conducting the experiments using the single IR sensor and a sliding mechanical device with the help of a non-integral interpolation method, the crops in the cotyledon stage could be precisely located.

Quality characteristics of noodles added with Houttuynia cordata Thunb. powder (어성초 분말을 첨가한 국수의 품질특성)

  • Park, Woo-Po
    • Food Science and Preservation
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    • v.21 no.1
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    • pp.34-39
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    • 2014
  • The purpose of this study was to evaluate appropriate concentrations of Houttuynia cordata Thunb. powder, which has various functions, to dried wheat flour noodles. The viscosity of the composite flour and cooking quality of the noodle samples were measured, and sensory evaluations were conducted in this study. When measured by the amylograph, the composite flour had lower viscosity in the gelatinization points, maximum viscosity, and viscosity at a temperature of $95^{\circ}C$ than those of control. However, the gap between the maximum viscosity and viscosity after 15 minutes at a temperature of $95^{\circ}C$ was not great among all of the samples. While cooked noodles containing H. cordata Thunb. powder had lower weight and lower volume than those of control, the weight of two cooked noodle samples with 0.5% and 1.5% of H. cordata Thunb. powder was insignificantly different. Cooked noodles had higher concentrations of H. cordata Thunb. powder; therefore, the L value of cooked noodles decreased, while a and b color values of the noodles increased. Color, flavor, taste, and overall acceptability of the samples with H. cordata Thunb. powder were lower than those of wheat flour noodles via sensory evaluation. In the overall acceptability, however, samples with 0.5% and 1.5% of H. cordata Thunb. powder were insignificantly different from control. As a result, it was finally suggested that optimum level of the H. cordata Thunb. powder in the product was below 0.5%.

Studies on NO, nNOS, eNOS, iNOS and NE Expression by Acupuncture at SP4, KI4 and LR5 (족삼음경의 락혈에 시술된 침 자극에 의한 NO, NOS, NE 발현 연구)

  • Lee, Yumi;Shin, Wook;Choi, Donghee;Kim, Mirae;Na, Changsu;Youn, Daehwan
    • Korean Journal of Acupuncture
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    • v.34 no.1
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    • pp.37-46
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    • 2017
  • Objectives : The acupuncture about acupoint affects the production of NO, NOS, and NE.Local action of acupuncture is important for acupuncture treatment. To prove this, the revelation degree of NO, NOS, and NE was observed by stimulating the acupuncture at the connecting point of SP4, KI4, and LR5 in the depths of Superficial layer, Middle layer and Deep layer. Methods : Needles were inserted into rats, on each right and left sides of the connecting point, SP4, KI4 and LR5 acupoints which are the stream points of the foot meridian. After insertion, needles were retained for three minutes. After the retention, rat was sacrificed via cardiac puncture, and tissues of each SP4, KI4 and LR5 point near meridian vessel was extracted to examine the changes in the expression of NO, NOS and NE. Results : In terms of the effect in NO production, there was significant increase in the Superficial layer, Middle layer and Deep layer at KI4. In terms of the effect in NE production, there was significant decrease in the Superficial layer at SP4 and increase in the Superficial layer, Middle layer and Deep layer at LR5. In terms of the effect in nNOS production, there was significant increase in the Superficial layer, Middle layer and Deep layer at SP4 also in the Superficial layer at KI4. In terms of the effect in eNOS production, there was a significant increase in the Superficial layer, Middle layer and Deep layer at SP4, KI4 and LR5. In terms of the effect in iNOS production, there was significant increase in the Superficial layer, Middle layer and Deep layer at SP4, KI4 and LR5. Conclusions : The effect of acupuncture applied at the connecting point of six meridians of the foot on the activities of NO, NOS and NE could be observed, and it can be induced from the effect of needle stimulation on disrupted local and systemic nervous responses.

Research for the Element to Analyze the Performance of Modern-Web-Browser Based Applications (모던 웹 브라우저(Modern-Web-Browser) 기반 애플리케이션 성능분석을 위한 요소 연구)

  • Park, Jin-tae;Kim, Hyun-gook;Moon, Il-young
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2018.10a
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    • pp.278-281
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    • 2018
  • The early Web technology was to show text information through a browser. However, as web technology advances, it is possible to show large amounts of multimedia data through browsers. Web technologies are being applied in a variety of fields such as sensor network, hardware control, and data collection and analysis for big data and AI services. As a result, the standard has been prepared for the Internet of Things, which typically controls a sensor via HTTP communication and provides information to users, by installing a web browser on the interface of the Internet of Things. In addition, the recent development of web-assembly enabled 3D objects, virtual/enhancing real-world content that could not be run in web browsers through a native language of C-class. Factors that evaluate the performance of existing Web applications include performance, network resources, and security. However, since there are many areas in which web applications are applied, it is time to revisit and review these factors. In this thesis, we will conduct an analysis of the factors that assess the performance of a web application. We intend to establish an indicator of the development of web-based applications by reviewing the analysis of each element, its main points, and its needs to be supplemented.

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A study on the Feasibility Analysis factor Model for Housing Development Projects (주택개발사업의 사업성분석인자 모델에 관한 연구;사업성 분석인자의 중요도 분석 및 타당성 검증을 증심으로)

  • Hong, Yeong-Geon;Kim, Young-Ai;Kim, Yong-Su
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • 2007.11a
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    • pp.249-254
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    • 2007
  • The main bodies of housing markets of today need to carry out a systematic and objective feasibility analysis even from the step of planning in order to succeed in a diverse and complex market environment. Therefore, it is meaningful to understand this housing market environment and propose a necessary model for the feasibility analysis of hosing business. In this vein, the aim of this study was to extract an actual and practical feasibility analysis factor and its importance for housing market, and then present and apply a feasibility analysis factor model to an on-site example, in order to verify the model's validity. For this, the investigator interviewed with and carried out a questionnaire survey of experts in housing development projects. Study findings are as follows:First, the feasibility analysis factor, derived in this study, could provide a ground to evaluate the feasibility of subject projects in the planning of development through an analysis index. Second, when feasibility is under the level of carrying out the projects, it is possible to reexamine the projects through extracting analysis factors of which points are under the standard and via a feedback process for improvable analysis factors. Therefore, the result of applying the feasibility analysis model of this study to actual housing development projects analytically shows that the model could provide a practical evaluation criterion to the person in charge of project development through an analysis index.

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Content Analysis of Voluntary Environmental Disclosure Made in Stand-alone Environmental Reports or Company Web-sites: Focusing on the Interrelations between Disclosure Quality, Environmental Performance and Economic Performance (환경보고서 혹은 웹사이트를 통한 자발적 환경공시의 내용분석: 환경성과 및 경제적 성과와의 동시적 상관관계를 중심으로)

  • Choi, Jong-Seo
    • Journal of Environmental Policy
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    • v.9 no.3
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    • pp.69-114
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    • 2010
  • This study investigates the voluntary corporate disclosure of environmental information via stand-alone environmental reports or company web-sites. Quality of disclosure was assessed using the content analysis index proposed by Clarkson et al. (2008) based on GRI guidelines. Descriptive statistics on disclosure scores by category suggest that the level of disclosure is low relative to the expectation implied by the GRI reporting guidelines, and points to the need for improvement in the future. Specific areas where improvement is required include the disclosure of environmental performance indicators. Corporate environmental performance was measured in terms of the percentage of toxic wastes that was treated or processed by each firm and economic performance, by industry-adjusted annual return, which was subject to a series of association tests designed to explore the interrelations among environmental disclosure, environmental performance, and economic performance. The individual equation approach based on OLS procedures suggests a positive association between environmental performance and the level of discretionary environmental disclosures, which is not the case between environmental and economic performance. An integrated analysis using simultaneous equations approach does not indicate any significant relationships among three constructs, suggesting the lack of endogeneity, inconsistent with Al-Tuwaijri et al. (2004). Additional analysis assesses the level of environmental disclosure made in footnotes to the audited annual reports, which suggests that the quality of disclosure is very low and that footnote disclosure does not serve as a meaningful channel for the provision of corporate environmental information.

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Implementation of a Static Analyzer for Detecting the PHP File Inclusion Vulnerabilities (PHP 파일 삽입 취약성 검사를 위한 정적 분석기의 구현)

  • Ahn, Joon-Seon;Lim, Seong-Chae
    • The KIPS Transactions:PartA
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    • v.18A no.5
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    • pp.193-204
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    • 2011
  • Since web applications are accessed by anonymous users via web, more security risks are imposed on those applications. In particular, because security vulnerabilities caused by insecure source codes cannot be properly handled by the system-level security system such as the intrusion detection system, it is necessary to eliminate such problems in advance. In this paper, to enhance the security of web applications, we develop a static analyzer for detecting the well-known security vulnerability of PHP file inclusion vulnerability. Using a semantic based static analysis, our vulnerability analyzer guarantees the soundness of the vulnerability detection and imposes no runtime overhead, differently from the other approaches such as the penetration test method and the application firewall method. For this end, our analyzer adopts abstract interpretation framework and uses an abstract analysis domain designed for the detection of the target vulnerability in PHP programs. Thus, our analyzer can efficiently analyze complicated data-flow relations in PHP programs caused by extensive usage of string data. The analysis results can be browsed using a JAVA GUI tool and the memory states and variable values at vulnerable program points can also be checked. To show the correctness and practicability of our analyzer, we analyzed the source codes of open PHP applications using the analyzer. Our experimental results show that our analyzer has practical performance in analysis capability and execution time.