• Advances in Traditional Medicine
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• 제10권4호
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• pp.310-318
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• 2010

Verbena officinalis (Verbenaceae) has been used as herbal medicine or health supplement in both Western and Eastern countries for centuries. In the present study, we examined the anti-inflammatory and antioxidant activities of the methylene chloride fraction of V. officinalis (VMC). To elucidate the anti-inflammatory properties of VMC, we investigated the inhibition effects of nitric oxide production in interferon-gamma (IFN-$\gamma$) and lipopolysaccharide-stimulated mouse peritoneal macrophages. VMC suppressed nitric oxide production, inducible nitric oxide synthase and cyclooxygenase-2 expression dose-dependently without notable cytotoxicity. In various radical scavenging assays, VMC exhibited strong scavenging effect on DPPH radical, superoxide radical, nitric oxide radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical. VMC also showed potent reducing power. These findings strongly suggest that VMC may be beneficial in oxidative stress-mediated inflammatory disorders.

• 한국조경학회지
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• 제46권1호
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• pp.96-105
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• 2018

허브식물을 이용한 옥상녹화시 관수를 실시한 처리구에서 좋은 생육특성을 보인 허브식물은 애플민트, 레몬밤, 스피아민트, 파인애플세이지, 초코민트, 옥스아이데이지, 캐모마일 및 타임이었다. 반면, 무관수 처리구에서 좋은 생육특성을 보인 허브식물은 페퍼민트, 장미허브 및 피버퓨였다. 관수에 따른 생장에 차이가 없는 허브식물은 라벤다, 로즈마리, 탠지, 레몬버베나, 헬리오트로프, 소프워트 및 레이디스맨틀이었다. 관수 및 무관수 처리가 허브식물의 생장에 미치는 영향과 최대 광합성 효율을 조사한 결과, 관수처리 시 생장이 증가하고 최대광합성효율이 증가한 허브식물로는 초코민트, 스피아민트, 레몬밤, 애플민트 등으로 나타났다. 무관수시 생장이 증가하고 최대광합성효율이 증가한 허브식물로는 페퍼민트와 탠지였으며, 장미허브는 무관수시 생장은 증가한 반면 최대 광합성효율은 감소하여 스트레스를 많이 받는 것으로 생각되었다. 또한, 관수 및 무관수 처리에 따른 생장 차이가 없으나, 최대 광합성효율이 증가한 허브는 로즈마리와 소프워트였으며, 레몬버베나, 피버퓨, 헬리오트로프 등은 관수 및 무관수에 따른 생장 차이가 없고, 최대 광합성 효율 역시 큰 차이가 없는 것으로 나타났다. 초코민트와 페퍼민트는 중부지방에서 월동 가능함과 동시에 겨울에도 잎이 지지 않고 상록으로 유지되어 옥상녹화에 적합한 식물이라고 생각되었다. 반면, 장미허브, 파인애플세이지, 애플민트는 추위에 약하고 월동이 안 되어서 옥상녹화에 부적합한 것으로 생각되었다. 스피어민트와 레몬밤은 엽색의 변화와 동해 피해로 인해 관상가치는 떨어졌으나, 월동이 가능하기 때문에 옥상녹화에 어느 정도 적용 가능한 식물로 판단되었다. 중부지방에서 활용 가능한 저관리형 옥상녹화용 허브식물로는 무관수시 생장이 증가하고 월동이 가능한 페퍼민트와 탠지가 저관리형 옥상녹화용 식물로 가장 적합한 것으로 나타났으며, 레몬버베나, 소프위트 및 레이디스맨틀도 중부지방에서 옥상녹화용 허브식물로 활용 가능한 것으로 판단되었다.

• 대한약침학회지
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• 제25권4호
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• pp.364-368
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• 2022

Objectives: Verbenalin is a compound found in herbs such as Cornus officinalis and Verbena officinalis. This study investigated whether verbenalin is safe by analyzing its mutagenicity. Methods: To examine the mutagenic potential of verbenalin, a bacterial reverse mutation test (Ames test) was conducted with Salmonella typhimurium and Escherichia coli strains. Experiments with and without metabolic activity were performed. Results: The mean colony number was less than double that of the control. Growth inhibition and precipitation of verbenalin were not apparent in all strains at different concentrations regardless of metabolic activity. Conclusion: Verbenalin did not show any signs of mutagenicity in this study. Additional toxicity studies including repeated oral toxicity, reproductive toxicity, and carcinogenicity tests are needed.

• Journal of Acupuncture Research
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• 제39권4호
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• pp.310-316
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• 2022

Background: Verbenalin is an iridoid glucoside, which is among the active components of some medicinal herbs such as Verbena officinalis Linn, and Cornus officinalis Siebold and Zucc. Previous studies have confirmed the antioxidant activity and neuroprotective potential of verbenalin. To confirm the safety of verbenalin, an approximate lethal dose was determined based on a single oral dose toxicity study. Methods: Institute of Cancer Research mice were randomly assigned to three verbenalin exposure groups (250, 500, and 1,000 mg/kg) and a control group (5% methylcellulose solution). There were (5 male and 5 female mice per group). Mortality, clinical signs, and body weight were monitored for 14 days, and necropsies were conducted. Results: No mortalities were observed in the control group or the verbenalin 250 mg/kg group, whereas mortalities were observed in the 500 mg/kg and 1,000 mg/kg verbenalin groups. During the observation period, stool abnormalities such as mucous stools were observed. Clinical signs such as loss of locomotor activity were observed in the 500 mg/kg and 1,000 mg/kg verbenalin groups. During the study period, significant changes in body weight were observed in the 500 mg/kg and 1,000 mg/kg verbenalin groups; however, no gross abnormalities were observed at necropsy. Overall, no toxicity was found in the 250 mg/kg group. Conclusion: The approximate lethal dose of verbenalin was estimated to be 500 mg/kg. For a more accurate assessment of the safety of verbenalin, other types of studies such as repeated-dose toxicity studies should also be conducted.

• 한국환경복원기술학회지
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• 제14권4호
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• pp.25-40
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• 2011

The vascular plants in Jeokjabong, Bogil Island were listed 350 taxa composed of 89 families, 233 genera, 309 species, 1 subspecies, 36 varieties and 4 forms. Divided into woody plants were 142 taxa (40.6%) and herbaceous plants were 208 taxa (59.4%). Based on the list of rare and endangered species by Korea Forest Service and Korea National Arboretum, 3 taxa were recorded; Celtis choseniana, Asarum maculatum, Rubus sorbifolius, and endemic plants, 10 taxa were recorded; Asarum maculatum, Cephalotaxus koreana, Carpinus coreana, Celtis choseniana, Lespedeza maritima, Indigofera koreana, Dendropanax morbifera, Ligustrum quihoui var. latifolium, Weigela subsessilis, Carex okamotoi, and plant species subject to permission for taking abroad, 4 taxa were recorded; Celtis choseniana, Asarum maculatum, Vaccinium oldhami, Carex okamotoi. Based on the list of floristic regional indicator plants by Korean Ministry of Environment were total 64 taxa (18.3% of all 350 taxa of vascular plants); Ligustrum quihoui var. latifolium in class IV, 19 taxa (Stauntonia hexaphylla, Cinnamomum japonicum, Zanthoxylum ailanthoides, Daphniphyllum macropodum, Centella asiatica, Verbena officinalis, Mitchella undulata, etc.) in class III, 5 taxa (Potentilla dickinsii, Ostericum melanotilingia, Sanicula rubriflora, Caryopteris incana, Teucrium veronicoides) in class II and 39 taxa (Gleichenia dichotoma, Cyrtomium fortunei, Aphananthe aspera, Rubus hirsutus, Meliosma myriantha, Hedera rhombea, Ainsliaea apiculata, etc.) in class I. Based on the list of naturalized plants, 5 families, 17 genera, 20 taxa (Phytolacca americana, Lepidium virginicum, Robinia pseudo-acacia, Verbesina alternifolia, Lolium perenne, etc.) and naturalization rate was 5.7% of all 350 taxa of vascular plants. Hereafter natural ecosystem into disturbance on naturalized plants and legal protection species and rare and endangered species distribute in the Bogil Island, precision investigation after management counterplan of the monitoring a point of view be required.