• 국제초고층학회논문집
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• 제3권4호
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• pp.273-278
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• 2014

Green roof's performance in reducing stormwater runoff has been reported by numerous studies. Nonetheless, the roles of low growing vegetation in influencing stormwater runoff reduction on green roofs have been greatly overlooked. This paper describes an experiment investigating the influence of low growing vegetation in the reduction of tropical stormwater runoff on extensive green roofs. Three types of locally occurring native vegetation and one non-native Sedum species were selected (fern, herb, grass and succulent) for the experiment. Stormwater runoff reduction performance from different low growing species was done by measuring excess water runoff from the simulated green roof modules. The results show significant differences in stormwater runoff reduction from different types of vegetation. Fern was the most effective in reducing stormwater runoff, followed by herb, Sedum and grass. Vegetative characters that are found to attribute towards the performance of stormwater runoff are rooting density, structure, density, leaf type, and vegetation biomass.

• 한국자원식물학회지
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• 제20권6호
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• pp.570-578
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• 2007

In order to investigate the basic vegetation information for the efficient management of the Sorbus commixta habitat in Korea, 31 plots in Mt. Chiri area were selected of which vegetation types were classified in phytosociological method and ecological characteristics were identified. As a result, the habitat was classified as S. commixta community group which was then subdivided to Prunus padus group and Picea jezoensis group. Prunus padus group was classified in Ainsliaea acerifolia subgroup and Magnolia sieboldii subgroup, while the Picea jezoensis group was classified in Rhododendron mucronulatum subgroup and Echinopanax horridum subgroup. Thus, the forest vegetation of S. commixta was classified in 1 community, 2 groups and 4 subgroups, and found to have 4 vegetation units in total.

• The Korean Journal of Ecology
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• 제25권4호
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• pp.267-273
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• 2002

Regeneration process of the Korean beech (Fagus multinervis Nakai) forest was studied in relation to the distributional pattern and age structure. In this study, it was clarified that the Korean beech forest is regenerated in both way: seedlings(sexual reproduction) and sprouts(vegetative reproduction) formations. In the large gaps, seedling may be established, resulting in clusters of even-aged individuals. But in the small-sized gaps, there may filled by the extention of surrounding trees and/or by the young trunks, which have sprouted from the base of stumps. As most gaps in the F. multinervis forest were derived from falling of a single stem, regeneration by sprouts seems to be more common than that by the seedlings.

• ALGAE
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• 제34권4호
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• pp.267-275
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• 2019

Three new species of the genus Micractinium were collected from five localities on the South Shetland Islands in maritime Antarctica, and their morphological and molecular characteristics were investigated. The vegetative cells are spherical to ellipsoidal and a single chloroplast is parietal with a pyrenoid. Because of their simple morphology, no conspicuous morphological characters of new species were recognized under light microscopy. However, molecular phylogenetic relationships were inferred from the concatenated small subunit rDNA, and internal transcribed spacer (ITS) sequence data indicated that the Antarctic microalgal strains are strongly allied to the well-supported genus Micractinium, including M. pusillum, the type species of the genus, and three other species in the genus. The secondary structure of ITS2 and compensatory base changes were used to identify and describe six Antarctic Micractinium strains. Based on their morphological and molecular characteristics, we characterized three new species of Micractinium: M. simplicissimum sp. nov., M. singularis sp. nov., and M. variabile sp. nov.

• 한국작물학회지
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• 제39권3호
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• pp.262-269
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• 1994

본 연구는 잡종 강세 정도가 다른 벼 F$_1$과 양친품종들의 생장 특성 및 다수성 요인을 구명하고자 Indica와 Japonica에서 각각 F$_1$의 잡종강세가 큰 것과 작은 것 그리고 이들의 양친 등 10개 품종을 공시하여 생육 시기별 생장 특성, 질소 함량과 공간적 분포, 흡광계수, 광합성 속도, 수량 및 수량 구성요소에 대하여 검토한 결과는 다음과 같다. 1. F$_1$의 잡종강세가 나타나는 수량 구성요소는 수수와 수당 영화수였으며, 단위 면적당 영화수는 양자의 Heterosis가 상승적으로 작용하여 가장 큰 잡종강세를 나타내었으며 등숙률과 천립중은 잡종강세 현상이 나타나지 않았다. 2. 출수기에 양친보다 상층부에 분포하는 잎이 많았으며 또한 광 소멸계수는 전 생육기간에 걸쳐서 양친보다 작아서 수광상태가 양호한 군락 구조를 형성하였다. 3. F$_1$은 양친에 비하여 잎의 질소함량이 높을 뿐만 아니라 군락 상하층간의 질소농도의 구배가 커서 군락 동화에 유리한 질소의 공간적 분포를 하였는데, 특히 생육 초기에 F$_1$과 양친간 질소의 공간적 구배의 차이가 컸으며 생육의 진전에 따라 그 차이는 작아졌다. 4. 유수 분화기 엽의 광합성 속도는 F$_1$이 양친 품종들보다 높았는데 이는 엽의 질소함량이 높은데서 기인되는 것으로 판단되었으며, 같은 품종군에서는 수량의 Heterosis가 높은 조합이 광합성 속도와 질소함량이 높았다. 5. 유수분화기 이전 특히 생육초기의 군락생장율(CGR), 순동화율(NAR), 엽면적지수(LAI)는 양친에 비하여 잡종품종이 높았으며, 품종들의 이 생육지표들은 잎의 질소함량과 높은 정의 상관이 있었다. 한편 영양생장기의 이 생육지표들 및 질소 보유량과 품종들의 수량 및 단위 면적당 영화수와는 정의 유의한 상관이 있었으며 생육초기일수록 상관이 높았다. 6. F$_1$은 생육초기에 엽의 질소함량이 높아 단위동화율이 높을 뿐만아니라 질소의 군락내 상하간의 농도 구배가 크고 흡광계수가 낮아 군락의 광합성에 유리하고, 이에따른 영양 생장기 건물 생산성의 증대가 단위 면적당 영화수의 증대를 통해 수량의 증대를 가져오는 것으로 판단되었다.

• The Plant Pathology Journal
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• 제19권1호
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• pp.34-42
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• 2003

The ascomycete Magnaporthe grisea is a pathogen of rice blast and is known to form specialized infection structures called appressoria for successful infection into host cells. To understand the molecular mechanism underlying infection process, appressorium-related genes were identified through global approaches including EST sequencing, differential hybridization, and sup-pression subtractive hybridization. EST database was generated on >2,000 cDNA clones randomly selected from appressorium stage cDNA library. Large number of ESTs showed homology to known proteins possibly involved in infection-related cellular development (attachment, germination, appressorium formation, and colonization) of rice blast fungus. The 1051 ESTs showing significant homology to known genes were assigned to 11 functional categories. Differential hybridization and suppression subtractive hybridization were applied to identify genes showing an appressorium stage specific expression pattern. A number of genes were selected as up-regulated during appressorium formation compared with the vegetative growing stage. Clones from various cDNA libraries constructed in different developmental stages were arrayed on slide glass for further expression profiling study. functional characterization of genes identified from these global approaches may lead to a better understand-ing of the infection process of this devastating plant disease, and the development of novel ways to protect host plant.

• Journal of Microbiology
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• 제38권4호
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• pp.230-238
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• 2000

Class III chitin synthases in filamentous fungi are important for hyphal growth and differentiation of several filamentous fungi. A genomic clone containing the full gene encoding Chs4, a class III chitin synthase in Penicillium chrysogenum, was cloned by PCR screening and colony hybridization from the genomic library. Nucleotide sequence analysis and transcript mapping of chs4 revealed an open reading frame (ORF) that consisted of 5 exons and 4 introns and encoded a putative protein of 915 amino acids. Nucleotide sequence analysis of the 5'flanking region of the ORF revealed a potential TATA box and several binding sites for transcription activators. The putative transcription initiation site at -716 position was identified by primer extension and the expression of the chs4 during the vegetative growth was confirmed by Northern blot analysis. Amino acid sequence analysis of the Chs4 revealed at least 5 transmembrane helices and several sites for past-transnational modifications. Comparison of the amino acid sequence of Chs4 with those of other fungi showed a close relationship between P chrysogenum and genus Aspergillus.

• 한국산림과학회지
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• 제109권4호
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• pp.421-428
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• 2020

Tamarind (Tamarindus indica L.) is one of the multipurpose tree species distributed in the tropical and sub-tropical climates. It is an important fruit yielding tree that supports the livelihood and has high social and cultural values for rural communities. The vegetative, reproductive, qualitative, and quantitative traits of tamarind vary widely. Characterization of phenotypic and genetic structure is essential for the selection of suitable accessions for sustainable cultivation and conservation. This study aimedto examine the genetic relationship among the collected accessions of sweet, red, and sour tamarind by using Random Amplified Polymorphic DNA (RAPD) primers. Nine accessions were collected from germplasm gene banks and subjected to marker analysis. Fifteen highly polymorphic primers generated a total of 169 fragments, out of which 138 bands were polymorphic. The polymorphic information content of RAPD markers varied from 0.10 to 0.44, and the Jaccard's similarity coefficient values ranged from 0.37 to 0.70. The genetic clustering showed a sizable genetic variation in the tamarind accessions at the molecular level. The molecular and biochemical variations in the selected accessions are very important for developing varieties with high sugar, anthocyanin, and acidity traits in the ongoing tamarind improvement program.

• 생명과학회지
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• 제16권2호
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• pp.225-230
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• 2006

생육온도가 느타리버섯 자실체의 생육 및 조직에 미치는 영향을 검토하기 위하여 온도를 $7^{\circ}C,\;15^{\circ}C,\;23^{\circ}C$로 달리하여 생육시키고, 자실체의 특성 및 조직의 미세구조 변화를 관찰 한 결과, 생육온도가 높을수록 갓의 두께는 얇아지고, 대의 두께 및 대의 길이는 길어졌으며, 버섯의 갓 색깔이 백색에 가깝게 변화하였다. 생육온도에 따른 자실체의 미세구조변화는 생육온도가 높아질수록 주름에 있어서 담자포자의 비산이 빠르고, 노화현상이 두드러졌다. 생육온도별로 느타리버섯 대부위의 물리적 특성을 조사한 결과 생육온도가 높을수록 경도는 낮아졌는데 이는 세포의 노화 및 균사의 밀도가 낮았기 때문으로 생각된다. 또한 대의 세포학적인 특정으로 액포화가 두드러져 생육온도가 높을수록 생육일수는 빨라지나 그만큼 노화가 쉽게 일어남을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제3권3호
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• pp.146-155
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• 1993

A DNA fragment from an alkali-tolerent Bacillus sp., conferring strong promoter activity, was subcloned into the promoter probe plasmid pPL703 and the nucleotide sequence of this promoter region was determined. The sequence analysis suggested that this highly efficient promoter region containing the complex clustered promoters comprised three kinds of promoters (P1, P2 and P3), which are transcribed by $\sigma^B (formerly \sigma^{37}), \sigma^E(formerly \sigma^{29}) and \sigma^A (formerly \sigma^{43})$ RNA polymerase holoenzymes which play major rules at the onset of endospore formation, during sporulation and at the vegetative phase of growth, respectively. S1 nuclease mapping experiments showed that all three promoters had staggered transcription initiation points. The results of chloramphenicol acetyltransferase assay after the subcloning experiments also indicated that the expression of these clustered promoters was correlated with the programs of growth and endospore development. Promoter P1, P2 and P3 were preceded by 75% AT, 79% AT and 81% AT regions, respectively, and a partial deletion of AT-rich region prevented transcription from promoter P1 in vivo. Two sets of 5 -AGTGTT-3 sequences and inverted repeat sequences located around the promoter P1 were speculated as the possible cis acting sites for the catabolite repression in B. subtilis. In vivo transcripts from these sequence regions may be able to form a secondary structure, however, the possibility that a regulatory protein induced by the excess amount of glucose could be bound to such a domain for crucial action remains to be determined.