• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5391-5396
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• 2013

Aim: To investigate the role of Golgi phosphoprotein 3 (GOLPH3) in tumour growth and metastasis of esophageal squamous cancer. Methods: A lentiviral shRNA-vector was utilized to stably knockdown GOLPH3 in Eca-109 esophageal squamous cancer cells. mRNA transcription and protein expression of GOLPH3 were examined by real-time quantitative PCR and Western blotting, respectively. Cell proliferation activity was assessed by MTT assay and invasion and migration potentials by matrigel invasion and transwell motility assays. Results: Stable knockdown in the GOLPH3 cell line was established. PD-A gene expression was significantly suppressed by lentivirus-mediated RNAi, which resulted in reducing the capacity for cell proliferation, migration, invasion and adhesion in vitro. In vivo, GOLPH3 depletion resulted in inhibition of tumour growth, with stable decrease in the expression of GOLPH3 in tumor xenografts. Conclusions: Our findings suggest that lentivirus mediated silencing of the GOLPH3 gene has a significant anti-tumour effect on esophageal squamous cancer in vitro and in vivo. In addition, the results indicate that GOLPH3 might be an effective molecular target for gene therapy in esophageal squamous cancer.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.154-159
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• 2006

Interleukin-18 (IL-18), otherwise known as interferon-gamma-inducing factor (IGIF), is one of several well characterized and important cytokines that contribute to host defenses. The complementary DNA (cDNA) of mature human interleukin-18 gene (hIL-18) was fused with the signal peptide of the rice amylase 1A gene (Ramy1A) and introduced into the plant expression vector under the control of a duplicated CaMV 35S promoter. The recombinant plasmid was transformed into tobacco (Nicotiana tabacum L. cv Havana) using the Agrobacterium-mediated transformation method. The integration of the hlL-18 gene into the genome of transgenic tobacco plants was confirmed by polymerase chain reaction (PCR) amplification and its expression was observed in the suspension cells that were derived from the transgenic plant callus by using Northern blot analysis. The hlL-18 protein was detected in the extracts of the transgenic callus and in the medium of the transgenic tobacco suspension culture by using immunoblot analysis. Based upon enzyme-linked immunosorbant assay (ELISA) results, the expression level of the hlL-18 protein approximated $166{\mu}g/L$ in the suspension culture medium. Bioassay results from the induction of $interferon-{\gamma}$ from a KG-1 cell line indicated that the hlL-18 secreted into the suspension culture medium was bioactive.

• Journal of Power Electronics
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• 제17권5호
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• pp.1363-1371
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• 2017

In this paper, an improved Direct Power Control (DPC) algorithm is presented for grid connected three phase PWM rectifiers. The new DPC approach is based on two main tasks. First the optimization of the look-up table, which is well-known in conventional DPC, is outlined for selecting the optimum converter output voltage vectors. Secondly a very simple and effective method is used to directly calculate their duty cycles from the power errors. Therefore, the measured active and reactive powers are made to track their references using hysteresis controllers. Then two vectors are selected and applied during one control cycle to minimize both the active and reactive power ripples. The main advantages of this method are that there is no need of linear current controllers, coordinates transformations or modulators. In addition, the control strategy is able to operate at constant switching frequencies to ease the design of the power converter and the AC harmonic filter. The control exhibits a good steady state performance and improves the dynamic response without any overshoot in the line current. Theoretical principles of the proposed method are discussed. Both simulation and experimental results are presented to verify the performance and effectiveness of this control scheme.

• 대한전기학회논문지:전력기술부문A
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• 제49권6호
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• pp.259-265
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• 2000

The purpose of this paper is to detect and identify topological errors in order to maintain a reliable database for the state estimator. In this paper, a two stage estimation procedure is used to identify the topology errors. At the first stage, the WSAV state estimator which has characteristics to remove bad data during the estimation procedure is run for finding out the suspected branches at which topology errors take place. The resulting residuals are normalized and the measurements with significant normalized residuals are selected. A set of suspected branches is formed based on these selected measurements; if the selected measurement is a line flow, the corresponding branch is suspected; if it is an injection, then all the branches connecting the injection bus to its immediate neighbors are suspected. A new WLAV state estimator adding the branch flow errors in the state vector is developed to identify the branch topology errors. Sample cases of single topology error and topology error with a measurement error are applied to IEEE 14 bus test system.

• 한국산업융합학회 논문집
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• 제2권2호
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• pp.91-102
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• 1999

The paper describes a robust adaptive control algorithm for induction motor drive without speed sensor at low speed range. The control algorithm use only current sensors in a space vector pulse width modulation within loop control with rotor speed estimation and voltage source inverter. On-line rotor speed estimation is based on utilizing parallel model reference adaptive control system. MRAC of the modified flux model for flux and rotor speed estimator uses dual-adaptation mechanism, ${\omega}_r$ and ${\omega}_e$ scheme. The estimated flux components in the model can be compensated from the effects of offset errors on pure integrals. It can be compensated to the parameter variations and torque fluctuation with speed estimation in less then 10 rad/sec. In a simulation, the proposed induction motor control algorithm without speed sensor at very low speed range are shown to operate very well in spite of variable rotor time constant and fluctuating load without change the controller parameters. The suggested control strategy and estimation method have been validated by simulation study, and it proposed the designed system for the implementation using TI320C31 DSP/ASIC controller.

• 대한임상검사과학회지
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• 제43권3호
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• pp.89-97
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• 2011

The Human Papilloma Virus (HPV) infection has been strongly associated with pathogenesis of uterine cervix carcinoma. HPV type 16, a causative agent of uterine cervix carcinoma, encodes the E6 and E7 oncogenes, expression of which is pivotal for malignant transformation and maintenance of malignant phenotypes. To develop a gene therapy for HPV-related carcinoma, We investigated the effect of E6 short-interfering RNA (E6 siRNA) on the expression of this oncogene and on the growth of HPV 16-related uterine cervix carcinoma cells. SiHa cells, a uterine cervix carcinoma cell line, which contain a single copy of HPV 16 integrated in the chromosome and express the E6 and E7 oncogenes. Before 24 hr of transfection, cells were seeded and transfected with control plasmid or E6 siRNA-expressing plasmid. The mRNA was analysed by reverse transcriptase polymerase chain reaction (RT-PCR). The cell growth rate was investigated by MTT method. The E6 mRNA level in SiHa cells was decreased in HPV 16 E6 siRNA-expression vector transfected cells and a decrease in the growth of these cells was also observed. From these results. it is evident that E6 siRNA played a role in suppression of growth of SiHa cells and has a fair chance as a candidate for gene specific therapy for HPV related uterine cervix carcinoma.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2011년도 추계학술대회 초록집
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• pp.43.1-43.1
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• 2011

The detail simulation modeling of fully-fed induction generator is investigated through PC based MATLAB/Simulink environment. Generator's stator currents are controlled by indirect vector control method. In this method, generator side converter controls the maximum excitation (air gap flux) by stator d-axis current and controls generator torque by stator q-axis current. Induction generator speed is controlled by tip speed ratio (TSR) upon the wind speed variations in order to generate the maximum output power. The generator torque model is specified as a 3-blade wind turbine with rating, then, the model is simulated under normal operating condition and three different fault conditions. The matlab model designed for fully-fed induction generator based wind farm provides good performance under normal and grid fault conditions. It provides good results for different pwm techniques and fault conditions except the single-phase line to ground fault, which should be verified with real time data from wind farms.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 심포지엄 논문집 정보 및 제어부문
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• pp.293-294
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• 2007

This paper addresses the adaptive controller for efficiency optimization of induction motors. The paper describes an adaptive controller based on-line efficiency optimization control of a drive that uses a direct vector controlled induction motors. To improve the efficiency of the induction motors, it is important to find the optimal flux reference that minimize power loss. The proposed optimal flux reference is derived using a power loss function that is constructed with stator resistance losses, rotor resistance losses and core losses. The proposed sliding mode flux observer generates estimates the unmeasured rotor fluxes. An optimal efficiency controller has goal of maximizing the efficiency for a given speed and load torque. A simulation shows the effectiveness of the proposed technique.

• 정보처리학회논문지B
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• 제8B권4호
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• pp.343-350
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• 2001

백터 양자화기 설계는 다차원의 목적함수를 최소화하는 학습 알고리즘을 필요로 한다. 일반화된 Lloyd 방법(GLA)은 벡터 양자화기 설계를 위해 오늘날 가장 널리 사용되는 알고리즘이다. GLA 는 일괄처리(batch) 방식으로 코드북을 생성하며 목적함수를 단조 감소시키는 강하법(descent algorithm)의 일종이다. 한편 Kohonen 학습법(KLA)은 학습벡터가 입력되는 동안 코드북이 갱신되는 온라인 벡터 양자화기 설계 알고리즘 이다. KLA는 원래 신경망 학습을 위해 Kohonen에 의해 제안되었다. KLA 역시 GLA와 마찬가지로 강하법의 일종이라 할 수 있다. 따라서 이들 두 알고리즘은, 비록 사용하기 편리하고 안정적으로 동작을 하지만, 극소(local minimum) 점으로 수렴하는 문제를 안고 있다. 우리는 이 문제와 관련하여 simulated annealing(SA) 방법의 응용을 논하고자 한다. SA는 현재까지 극소에 빠지지 않고 최소(global minimum)로 수렴하면서, 해의 수렴이 (통계적으로) 보장되는 유일한 방법이라 할 수 있다. 우리는 먼저 GLA에 SA를 응용한 그 동안의 연구를 개괄한다. 다음으로 온라인 방식의 벡터 양자화가 설계에 SA 방법을 응용함으로써 SA 방법에 기초한 새로운 온라인 학습 알고리즘을 제안한다. 우리는 이 알고리즘을 OLVQ-SA 알고리즘이라 부르기로 한다. 가우스-마코프 소스와 음성데이터에 대한 벡터양자화 실험 결과 제안된 방법이 KLA 보다 일관되게 우수한 코드북을 생성함을 보인다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1995년도 하계학술대회 논문집 B
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• pp.591-593
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• 1995

Artificial neural network based pattern recognition method is one of the most probable candidate for on-line power system transient stability analysis. Especially, Kohonen layer is an adequate neural network for the purpose. Each node of Kehonen layer competes on the basis of which of them has its clustering center closest to an input vector. This paper discusses Kohonen's LVQ(Learning Victor Quantization) and points out a defection of the algorithm when applied to the transient stability analysis. Only the clustering centers located near the decision boundary of the stability region is needed for the stability criterion and the centers far from the decision boundary are redundant. This paper presents a new algorithm ratted boundary searching algorithm II which assigns only the points that are near the boundary in an input space to nodes or Kohonen layer as their clustering centers. This algorithm is demonstrated with satisfaction using 4-generator 6-bus sample power system.