• Journal of Life Science
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• v.23 no.2
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• pp.167-174
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• 2013

Peroxiredoxin 6 (Prx 6) is a member of the thiol-specific antioxidant protein family, which may play a role in protection against oxidative stress and in regulating phospholipid turnover. The aim of this study was to determine whether a human Prx 6/Luc vector was stably expressed and responded to antioxidants in a lung cell line (NCI-H460). To achieve this, the luciferase signal, hPrx 6 mRNA expression, and superoxide dismutase (SOD) activity were measured in transfectants with a hPrx 6/Luc plasmid after treatment with four antioxidant extracts, including Korea white ginseng (KWG), Korea red ginseng (KRG), Liriope platyphylla (LP), and red Liriope platyphylla (RLP). First, the hPrx 6/Luc plasmid was successfully constructed with DNA fragments of human Prx 6 promoter, amplified by PCR using genomic DNA isolated from NCI-H460 cells, and cloned into the pTransLucent reporter vector. The orientation and sequencing of the hPrx 6/Luc plasmid were identified with restriction enzyme and automatic sequencing. A luciferase assay revealed significant enhancement of luciferase activity in the four treatment groups compared with a vehicle-treated group, although the ratio of the increase was different within each group. The KRG- and LP-treated groups showed higher activity than the KWG- and RLP-treated groups. Furthermore, the luciferase activity against RLP occurred roughly in a dose-dependent manner. However, the level of endogenous hPrx 6 mRNA did not change in any group treated with the four extracts. The SOD activity was in agreement with the luciferase activity. Therefore, these results indicate that the hPrx 6/Luc vector system may successfully express and respond to antioxidant compounds in NCI-H460 cells. The data also suggest that the Prx 6/Luc vector system may be effectively applied in screening the response of hPrx 6 to antioxidant compounds in transgenic mice.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.287-292
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• 2000

Recombinant Aspergillus niger was constructed to express and secrete a biologically active murine granulaocyte macrophage-colony stimulating factor (mGM-CSF). A 500 bp fragment encoding the signal peptide and terminator of glyceraldehyde-3-phosphate dehydrogenase (gpd). The hygromycin phosphotrasferase gene (hph) was used as a selection marker for the fungal transformants. An expression vector was introduced into A. niger ATCC 9642, and a Northern blot analysis indicated the presence of a considerable amount of transcripts from the introduced mGM-CSF. The biological activity of recombinant mGM-CSF (rmGM-CSF) isolated from the culture filtrate was confirmend by measuring the proliferationof the GM-CSF dependent FDC-P1 cell line. It appeared that rmGM-CSF was amenable to the proteolytic activity produced by A. niger, since biological actibity was only observed when the transformants were grown in a protease-repressing medium, and the activity of rmGM-CSF dramatically decreased with an increase of age of the culture. The yield of rmGM-CSF, as determined by ELISA. was 640 ng/l of culture filtrate. Accordingly, its specific activity is estimated to be approximately two-and-a-half times higher than that of a commercial preparation from E. coli.

• 제어로봇시스템학회:학술대회논문집
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• 1997.10a
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• pp.1876-1879
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• 1997

This paper developes an efficient algorithm for the minimum distance calculation between general polyhedra(convex and/or concave). The polyhedron approximates and object using flat polygons which composed of more than three veritices. The algorithm developed in this paper basically computes minimun distance betwen two convex polygons and finds a set of polygons whcih makes a global minimum distance. The advantage of the algorithm is that the global minimum distance can be computed in any cases. But the big disadvantage is that minimum distance computing time is repidly increased with the number of polygons which used to approximate an object. This paper developes a method to eliminate unnecessary sets of polygons, and an efficinet algorithm to compute a minimum distance between two polygons in order to compensate the inherent disadvantage of the algorithm. It takes only a few times iteration to find minimum distance for msot polygons. The correctness of the algortihm are visually tested with a line which connects two points making a global minimum distance of simple convex object(box) and concave object(pipe). The algorithm can find minimum distance between two convex objects made of about 200 polygons respectively less than a second computing time.

• Journal of Institute of Control, Robotics and Systems
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• v.21 no.12
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• pp.1109-1114
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• 2015

This paper presents an improved learning-based visual inspection method for auto parts inspection in severe lighting changes. Automobile sunroof frames are produced automatically by robots in most production lines. In the sunroof frame manufacturing process, there is a quality problem with some parts such as volts are missed. Instead of manual sampling inspection using some mechanical jig instruments, a learning-based machine vision system was proposed in the previous research[1]. But, in applying the actual sunroof frame production process, the inspection accuracy of the proposed vision system is much lowered because of severe illumination changes. In order to overcome this capricious environment, some selective feature vectors and cascade classifiers are used for each auto parts. And we are able to improve the inspection accuracy through the re-learning concept for the misclassified data. The effectiveness of the proposed visual inspection method is verified through sufficient experiments in a real sunroof production line.

• Bulletin of the Korean Chemical Society
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• v.33 no.8
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• pp.2567-2573
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• 2012

We successfully synthesized $Fe_3O_4@SiO_2$ nanoparticles with ultrathin silica layer of $1.0{\pm}0.5$ nm that polyethyleneimine (PEI) with low molecular weight of 2.0-4.0 kDa was covalently conjugated with the resulting $Fe_3O_4@SiO_2$ nanoparticles by silane coupling reaction. The PEI-$Fe_3O_4@SiO_2$ nanoparticles were further used as gene delivery vector for a human fibroblast cell (IMR-90) line. Gene transfection efficiency of the PEI-$Fe_3O_4@SiO_2$ complexes did not increase remarkably after magnetofection; however, the addition of Lipofectamine 2000 significantly increased the transfection efficiency of the PEI-$Fe_3O_4@SiO_2$ complexes. We believe that the present approach could be utilized for magnetofection as alternative to $Fe_3O_4$ nanoparticles conjugated with the PEI of high molecular weight thanks to its relatively low cytotoxicity and high transfection efficiency.

• Journal of Electrical Engineering and Technology
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• v.11 no.6
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• pp.1769-1776
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• 2016

For on-line partial discharge (PD) monitoring of rotating machines, a novel sensor is proposed, which can be installed on the power lead inside the terminal box of the machine. The sensor has been designed to have high capacitance, and minimal reflection of measured pulses. As a sensitivity of the sensor, transfer impedance $Z_t$ has been measured and compared to conventional coupler-type sensors. A simple method is presented for measuring $Z_t$ of coupler sensors, using a vector network analyzer and a practical lead-cable of rotating machine. Through this method, it became possible to measure the $Z_t$ of coupler sensors including the installation environment of them. The $Z_t$ of the proposed sensor is higher than that of same sized other conventional couplers at frequencies between 30 and 92 MHz. Another sensitivity test has been performed using a PD calibrator as a test pulse source. The proposed sensor has higher measured peak voltage than the conventional coupler type sensors when the same charges were input.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.1 s.173
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• pp.182-189
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• 2000

This paper presents the real time control method of 5-axis NC machine for high precision and productivity based on the curvature theory, of a ruled surface. The trajectory, of NC machine is described by, way of a ruled surface generated by the points on part surface and tool axis direction vector. The curvature theory, of a ruled surface is then applied to deter-mine the motion parameters of the 5-axis machine for control. The controller computes position, orientation, and differential motion parameters of the tool in each sampling period. The real-time approach produces smoother surfaces and requires substantially less machining time compared to conventional off-line approaches. The propose real-time control method based of the curvature theory of a ruled surface may give new methodology of precision 5-axis machine control.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.22 no.3
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• pp.688-694
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• 1998

The ability to locate the defects in materials is one of the major attrations of the acoustic emission(AE) technique. The most conventional method for planar AE source localization is to place three or more AE sensors on the plate and to determine the source position by measuring the differences in the arrival times of the AE wave at the sensors, which is called as triangulation method. But this method can not be applied in the material of which elastic wave propagtion velocity is not known. In this paper, we propose two methods, vector method and error minimization method, for AE source location on the material with unknown AE wave velocity. In this method, it is not needed to know the propagation velocity previously, that is, we can apply this method to arbitrary material of which properties are not known exactly. Also, in this paper, the robustness to the error in the measurement of time differences are discussed for both methods. Finally, in order to evaluate the actual performances, experiments using a pencil lead break as the AE source were carried out on the aluminum plate.

• Journal of The Korean Digital Architecture Interior Association
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• v.12 no.1
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• pp.89-98
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• 2012

While the housing transaction price of Seoul tends to be stagnant or declining in line with the housing market recession since 2007, the jeonse price keeps continual increase. Such flow of jeonse price change has a serious influence on ordinary person's housing stability seriously. Therefore, it is very meaningful in terms of social policy to analyze the trend of recent jeonse price change. This study aims to have an empirical analysis of structural characteristics of the trend of recent jeonse price change. After the review of various previous studies, this study selected housing jeonse price index, non-sold house quantity, jeonse vs. transaction price rate, and housing construction performance as analytical variables, and employed monthly time series resources from January 2007 to April 2011. As a result, when the housing supply reduced, the potential quantity for jeonse market reduced that occurred unbalance of supply and demand in jeonse market. In turn, it caused the increase of jeonse price. And, in case of jeonse vs. transaction price rate change, the rate increased which means the increase of required rate of return of invested demand. As such, the increase of market risk degenerates the investment sentiment which caused the reduction of quantity for jeonse market as a submarket.

• Biomedical Science Letters
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• v.10 no.2
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• pp.65-73
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• 2004

I report that single-stranded antisense as a part of large circular (LC-) genomic DNA of recombinant M13 phage exhibits enhanced stability, sequence specific antisense activity, and no need for target site search. A cDNA fragment (708 bp) of rat TNF-$\alpha$ was inserted into a phagemid vector, and TNF-$\alpha$ antisense molecules (TNF$\alpha$-LCAS) were produced as single-stranded circular DNA. When introduced into a rat monocyte/macrophage cell line, WRT7/P2, TNF$\alpha$-LCAS was able to ablate LPS-induced TNF-$\alpha$ mRNA to completion. The antisense effect of TNF$\alpha$-LCAS was shown to be sequence-specific because expressions of three control genes ($\beta$-actin, GAPDH and IL-1$\beta$) were not significantly altered by the antisense treatment. Further, TNF$\alpha$-LCAS was found to be highly efficacious as only 0.1 $\mu$g (0.24 nM) of TNF$\alpha$-LCAS was sufficient to block TNF-$\alpha$ expression in 1$\times10^5$ WRT7/P2 cells. I have also observed specific antisense activity in reduction of NF-$\kappa$B gene expression. The results suggest that an antisense sequence as a part of single-stranded circular genomic DNA has a specific antisense activity.