• KSBB Journal
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• 제16권6호
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• pp.579-591
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• 2001

The cell growth inhibitor effect of cervical cancer cells was investigated by liposome mediated transfection (pRcCMVp53/lipofectin) and by transfection using adenovirus (AdCMVp57). The papilloma virus cancer cell lines we used in this study were HPV16 positive, having inhibiter gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3. LacZ gene of E.coli was used as the marker gene for the transfection efficiency. The effect on the inhibition of tumor cell growth was measured by cell count and cell viability though ELISA analysis and MTT assay. The inhibition of tumor cell growth was confirmed by measuring each assay for six days, comparing with the normal control cell growth. The cell growth of cervical cancer calls by transfection was significantly reduced and showed tittle differences among the cell lines. To eliminate the potential problem of Ad(adenovirus) contamination during rAAV production, rAAV can be produced by a triple transfection of vector plasmic, packaging plasmid, and adenovirus helper plasmid. To examine the helper functions of Ad plasmids on the production of rAAV vector, we carried out cotransfection of three plasmids, AAV vector, packaging construct, and Ad helper plasmids. The optimized transfection condition for calcium phosphate method is 25ug of total DNA per 10-cm-diameter plate of 293 cell. We found that rAAV yields peaked at 48hr after Ad infection. The titer of rAAV was measured by the dot blot analysis to measure the number of particles/ml based on the quantification of viral DNA. Recent1y, Kombucha(fungi) was identified as a very potent antileukefic agent. In the present study, effect of natural toxin(plankton) and Kombucha is PSP(GTXI-3, neoSTX), on various MTT assay cervical cancer cell line. Toxin(GTX 1-3, neoSTX) also inhibited the proliferation in primary cervical cancer calls in a dose-dependent toxin concentration. These results showed that toxin was very potent in inhibiting the proliferation of cervical cancer calls in vitro. Toxins and Kombuoha exhibited a dose dependent inhibition of cellular proliferation in cancer cell line.

• Animal cells and systems
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• 제6권4호
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• pp.317-322
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• 2002

To improve conventional yeast one-hybrid screening, we have developed an efficient mammalian one-hybrid system that allows rapid isolation of com-plementary DNAs which are able to induce human p14$^{ARF}$. tumor suppressor gene. A 1.5 kb promoter region of p14$^{ARF}$ was fused to EGFP to generate ARF promoter-EGFP reporter vector. This reporter plasmid was stably trans-fected into NIH3T3 cells for generation of reporter cell line. When the reporter cell line was infected with E2F-1 together with excess amounts of empty vector, the cells that received the positive modulator were readily identifiable by green fluorescence using FACS. The GFP-positive cells were cloned directly from the cultured cells and expanded in bulk culture. The genomic DNAs from GFP-positive cells were prepared and the CDNA insert in integrated retroviral genome was recovered by PCR using primers annealing to the retroviral vector sequences flanking the insert-cloning site. This system should be useful for efficient screening of expression CDNA libraries in mammalian cells to identify novel upstream regulators for spe-cific genes by one-hybrid interaction.ion.

• Tuberculosis and Respiratory Diseases
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• 제44권6호
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• pp.1271-1284
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• 1997

연구배경 : 종양괴사인자(tumor necrosis factor ; TNF)는 다양한 생물학적 기능을 가지고 있는 바, 그 중 생체 외에서 증명된 뚜렷한 항암 효과로 말미암아 최근 항암 유전자요법의 중요한 대상으로 관심을 모으고 있다. 현재 유전자 이입의 기술적 문제로 생체 외에서 암세포에 유전자 이입을 시행한 후 이를 다시 환자의 생체내로 이식하는 방법이 연구의 주종을 이루고 있다. 그러나 저자들의 과거의 연구를 포함한 여러 연구에서 TNF가 이입된 암세포는 TNF에 대해 내성을 보이는 것으로 증명되었고 이에는 새로이 방어 단백질을 합성하는 것이 관여할 것이라는 시사가 있었다. 이 획득내성의 기전을 밝히는 것이 종양생물학의 이해를 넓히고 보다 효과적인 항암 유전자요법을 개발하기위한 매우 중요한 과제로 생각된다.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2001년도 Proceedings ICPE 01 2001 International Conference on Power Electronics
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• pp.795-802
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• 2001

The commutation and control in matrix converters is more complicated as in voltage source converters. Natural freewheeling paths do not exist and the theoretic absent storage elements result in a direct coupled system of load and line currents as well as voltages. The paper offers an overview about staggered commutation and control policies in matrix converters. Based on the knowledge about load current direction and the signs of the line to line input voltages different multi-step commutation policies were derived. This paper examines the application of that policies in the case of space vector modulation and direct control methods with the focus on the resulting effects to the reference output voltage deviation.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1994년도 봄 학술발표회 논문집
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• pp.95-102
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• 1994

A path-switching strategy by combining the use of generalized inverse and line search is proposed. A reliable predictor for the tangent vector to bifurcation path is first computed by using the generalized inverse approach. A line search in the direction of maximum gradient of total potential at the point of intersection between the above predictor and a constant loading plane introduced in the vicinity of the detected bifurcation point is then carried out for the purpose of obtaining an improved approximation for a point on bifurcation path. With this approximation obtained, an actual point on bifurcation path is then computed through iteration on the constant loading plane.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2011년도 제42회 하계학술대회
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• pp.1099-1100
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• 2011

This study deals with the dynamic characteristics analysis of Linear Induction Motor (LIM) using finite element method in which the moving mesh technique is considered. The focus of this paper is to show the appropriate of on-line observer system for position sensorless control of a LIM under the phase asymmetry, saturation and iron loss. Comparisons are given with angle of the observer and that of proposed FEA method of linear induction motor, respectively. The position sensorless control system is realized, and the effective of the observer system is verified by experimental results.

• 한국항행학회논문지
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• 제15권5호
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• pp.925-930
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• 2011

본 연구는 온라인 쇼핑몰에서 사용자의 구매 선호도를 적용하는 방안을 제시하였다. 선호도를 분석하기 위하여 토픽 선호도 벡터가 사용되었으며, 각 토픽은 물품의 구매횟수를 이용하였다. 제안된 구조에서 각 토픽들의 연관은 상품구매 횟수를 나타내는 Purchase Hit, 관심물품에 대한 기존의 다른 사용자들의 구매 횟수를 나타내는 Count, 상품 선호도를 나타내는 Preference, 상품에 대한 정보를 나타내는 product를 구성하였다. 이러한 구조와 방법에 의하여, 제안된 방법은 사용자의 선호도가 반영된 상품을 효율적으로 제시할 수 있었다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 Techno-Fair 및 추계학술대회 논문집 전기물성,응용부문
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• pp.103-104
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• 2008

this paper describes the electric field distribution analysis along a shield gap inner vacuum interrupter(VI). The equipotential line and electric field and field vector in a VI are analysed by a finite element method and experiment at shield gap. in result, The equipotential line and electric field distribution was affected to VI shield gap. The reason is as it gets distortion of equipotential line done. finally, this paper recognized whether or not affected, and proposed gap with the most suitable shield length and an external insulation.

• 대한전기학회논문지:전력기술부문A
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• 제51권9호
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• pp.460-466
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• 2002

A reliable fault phase selection algorithm plays a very important role in transmission line protection, Particularly in Extra High Voltage (EHV) networks. The conventional fault phase selection algorithm used the phase difference between positive and negative sequence current excluding load current. But, it is difficult to pick out only fault current since we can not know when a fault occurs and select the fault phase in weak-infeed conditions that dominate zero-sequence current in phase current. The proposed algorithm can select the accurately fault phase using the sum of unit vectors which are calculated by positive-sequence voltage and negative-sequence voltage.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1997년도 춘계학술대회 논문집
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• pp.323-325
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• 1997

Conventionally it is asummed that the microstrip line conductor has a rectangular cross-section. but the additive and substactive processes used to create conductors for PCBs produce a conductor of approximately Trapezoidal cross-section. For wide Strip line, the thickness and edge effect will be small since most of capacitance is parallel plate rather than fringing and we can ignore the cross-section. For narrow strip lines, the edge effect become immportant. So in this paper, we measure the chracteristic impedance of microstripline by Vector Analyzer and simulate the electromagnetic field of microstripline using finite element method with edge angle.