Sun Young Gu;Su Jung Lee;So eun Lee;Chae Young Park;Jung Mi Lee;Inju Park;Yun Mi Chung;Gui Hyun Jang;Guiim Moon
Journal of Food Hygiene and Safety
/
v.38
no.3
/
pp.79-88
/
2023
Trifludimoxazin is a triazinone herbicide that inhibits the synthesis of protoporphyrinogen oxidase (PPO). The lack of PPO damages the cell membranes, leading to plant cell death. An official analytical method for the safety management of trifludimoxazin is necessary because it is a newly registered herbicide in Korea. Therefore, this study aimed to develop a residual analysis method to detect trifludimoxazin in five representative agricultural products. The EN method was established as the final extraction method by comparing the recovery test and matrix effect with those of the QuEChERS method. Various sorbent agents were used to establish the clean-up method, and no differences were observed among them. MgSO4 and PSA were selected as the final clean-up conditions. We used LC-MS/MS considering the selectivity and sensitivity of the target pesticide and analyzed the samples in the MRM mode. The recovery test results using the established analysis method and inter-laboratory validation showed a valid range of 73.5-100.7%, with a relative standard deviation and coefficient of variation less than 12.6% and 14.5%, respectively. Therefore, the presence of trifludimoxazin can be analyzed using a modified QuEChERS method, which is widely available in Korea to ensure the safety of residual insecticides.
Korean Journal of Agricultural and Forest Meteorology
/
v.18
no.3
/
pp.99-108
/
2016
The shuttle breeding of Korean winter wheat has been able to develop high-yielding and day-length-intensive varieties with a wide range of ecologic adoption. However, the phenology of winter wheat has been changed due to recent rises in the winter temperature of Toluca and increasing frequency of high temperatures. We defined two wheat groups (group II and III) with vernalization and evaluated the impact of cold exposure duration and heading ratio due to changes in sowing dates by measuring changes in cold exposure duration and corresponding heading states of each group. The wheat cultivars were sown on three dates in two years. The cold exposure requirement of wheat sown on 6 November 2013 was unfulfilled. The cold exposure requirement of wheat sown on 22 November and 6 December 2013 was fulfilled. However, in 2014, the cold exposure requirement of wheat sown on 5 and 20 November was fulfilled, but that of wheat sown on 5 December was unfulfilled. The differences for the two early November sowings were because winter temperature rises, which caused high temperatures in 2013, whereas early November 2014 saw normal temperatures for the area. The heading ratio of group II did not show a clear difference among the three sowing dates, while the heading ratio of group III was reduced by about half. This implies that the efficiency of shuttle breeding of group III will be high since it showed strong sensitivity to changes in sowing dates. We calculated future sowing dates of each group under near future climate scenarios; the future available sowing dates of group II were projected, but the dates of group III were never estimated in the temperature rise scenario in Toluca. Our findings suggest that change of sowing dates should be considered in the strategy for shuttle breeding of Korean winter wheat.
Kim, Dong Won;Kim, Chang Suk;Choi, Ki Young;Jeon, Young Shin;Jeong, Young Kyu;Yoon, Yeo Hag;Hon, Choon Pyo
Analytical Science and Technology
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v.6
no.5
/
pp.463-469
/
1993
HDBPDA ion exchange resin, {(4, 5) : (13, 14)-Dibenzo-6, 9, 12-trioxa-3, 15, 21, -triazabicyclo [15. 3. 1]heneicosa-(1, 17, 19)(18, 20, 21) triene ion exchange resin : HDBPDA ion exchange resin} had a capacity of 3.8meq/g dry resin. The distribution coefficients of alkali and alkaline earth metal ions on HDBPDA and strongly acidic cation exchange resin, Dowex 50W-X8(200-400mesh) in water, and the various concentrations of hydrochloric acid were determined. Concentration of hydrochloric acid have almost not influenced on the distribution coefficients of alkali and alkaline earth metal ions for the HDBPDA ion exchange resin, but generally the distribution coefficient slightly increased with decreasing concentration of hydrochloric acid. The distribution coefficients of metal ions in water are larger than those in various hydrochloric acid concentration. The distribution coefficients of alkali and alkaline earth metal ions for the Dowex 50W-X8 ion exchange resin increased with decreasing hydrochloric acid concentration. Especially, the distribution coefficients of alkaline earth metal ions increased rapidly compared to those for alkali metal ions. The distribution coefficients of alkali and alkaline earth metal ions on HDBPDA ion exchange resin increased in a linear manner with decreasing acid concentration, and the slope, d log Kd/d log $M_{HCl}$ is about -0.2. Of the distribution cofficients of alkali metal ions on Dowex 50W-X8, at range of moderate hydrochloric acid concentration, the slope is about -1, while the slope for alkaline earth metal ions is about -2. However, at very low hydrochloric acid concentration, the linear variation between distribution coefficient and acid concentration was not occurred, but the slope was deviated from above values at low acid concentration.
This study aimed to examine was conducted to the ability of microclimate control in old pine forests by surveying pine forest in Buddhist temples, where the pine forest are stably growing through active protection in the Gyeongnam region, and comparing variation characteristics of microclimate characteristics (temperature and humidity) and distribution of vegetation type. The study sites were pine forests protected well by Buddhist temples (Haein-sa, Beomeo-sa, Tongdo-sa, and Bulguk-sa) in the southeast region of Korea and thus known for stably growing young pine trees. According to the vegetation distribution status analysis, these pine forests did not have a high ratio of pine trees. Except for Tongdo-sa, the ratio of deciduous forest and mixed (deciduous and pine trees) forest had a much larger presence than that of pine forest. Measured data of microclimate showed that the Tongdo-sa area had significantly different characteristics compared to the other three areas. Tongdo-sa area showed a significantly higher diurnal range of temperatures and humidity than the other three areas, in both spring and summer. It is due to the difference in vegetation management. The forests around Tongdo-sa are mostly pine forests, except for the developed areas, while those in the other three areas have a dominant ratio of deciduous brad-leaved forests. Intensive control of pine forest is not effective in mitigating microclimate, i.e., temperature and air humidity. Stress caused by rising temperatures and decreasing air humidity is blamed for the decline of pine forests. Thus, the current active management of pine forests, such as the Tongdo-sa case, has been found to have a greater negative impact on the temperature and humidity stress. Therefore, we believe that a new change in forest management is necessary to increase the effect of mitigating the microclimate of pine forests.
This study was conducted to investigate the changes in moth occurrence of striped rice barer, Chilo suppressalis (Walker), in relation to climatic factors, rice varieties, and cultural practices. The light trap data from 41 forecasting stations for 14 years from 1966 to 1979 were analyzed by means of the changes in the time and amount of borer occurrence in connection with the introduction of new rice varieties, the accumulated effective day-degree and its variation for completing one generation, and the climatic factors affecting the moth occurrence. The total number of moths caught by light traps in both spring and summer generations were considerably decreased with the wide cultivation of new rice varieties. In fact, the spring moths were remarkably decreased since the new varieties were introduced in 1972. The occurrence ratio of summer moths against the preceeding spring moths was higher in the middle region and middle southern mountainous area than the other regions. Its high ratio of regions was annually expanded from the middle region to the southern region. The $50\%$ emergence dates of both generations were later in the southeastern region than in the middle region. The ecological characteristics were clearly shown between the northern and southern region of Chupungryeong in terms of the occurrence of summer moths, the ratio of occurrence of summer moths to the preceeding spring moths, and $50\%$ emergence dates of the summer moths during the years of $1977\~1979$. The ratio of the summer moth occurrence to the preceeding generation was negatively correlated with the average temperature in lune and July, respectively, and the average precipitation in late June. The ratio of spring moth occurrence over the preceeding generation was positively correlated with the average temperature in September, October, November, and March, respectively, whereas it was negatively correlated with the average precipitation in early September and March, and the average humidity in early May. The effective day-degree for one generation was in the range from 600 to 900 DD at upper threshold $30^{\circ}C$ and lower threshold $10^{\circ}C$.
It is difficult to analyze pymetrozine in citrus fruits using the hydromatrix method because of its low efficiency of purification and overlap of matrix and pymetrozine peaks. Liquid-liquid extraction can analyze pymetrozine in citrus fruits using dichloromethane. Since low pH interferes with the extraction of pymetrozine, the extracts of citrus fruits were maintained over pH 7.0 by adding borax buffer and 1 N NaOH in the improved method. According to the improved method, citrus fruits (such as lemon, lime, orange, tangerine, and grapefruit) were extracted and purified for HPLC-photo diode array analysis. The results of validation were as follows: $4.360{\mu}g/kg$ of limit of detection, $14.533{\mu}g/kg$ of limit of quantitation, and 0.007 mg/kg of method quantitative limit. Citrus fruits spiked with pymetrozine showed a recovery range from 71.8 to 83.7% and a coefficient of variation below 6%. Thus, the improved method can efficiently analyze pymetrozine in citrus fruits.
An analytical method was developed for the determination of quinoxyfen in agricultural products using the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted with 1% acetic acid in acetonitrile and water was removed by liquid-liquid partitioning with $MgSO_4$ (anhydrous magnesium sulfate) and sodium acetate. Dispersive solid-phase extraction (d-SPE) cleanup was carried out using $MgSO_4$, PSA (primary secondary amine), $C_{18}$ (octadecyl) and GCB (graphitized carbon black). The analytes were quantified and confirmed by using LC-MS/MS in positive mode with MRM (multiple reaction monitoring). The matrix-matched calibration curves were constructed using six levels ($0.001-0.25{\mu}g/mL$) and the coefficient of determination ($R^2$) was above 0.99. Recovery results at three concentrations (LOQ, 10 LOQ, and 50 LOQ, n=5) were in the range of 73.5-86.7% with RSDs (relative standard deviations) of less than 8.9%. For inter-laboratory validation, the average recovery was 77.2-95.4% and the CV (coefficient of variation) was below 14.5%. All results were consistent with the criteria ranges requested in the Codex guidelines (CAC/GL 40-1993, 2003) and Food Safety Evaluation Department guidelines (2016). The proposed analytical method was accurate, effective and sensitive for quinoxyfen determination in agricultural commodities. This study could be useful for the safe management of quinoxyfen residues in agricultural products.
Gu, Sun Young;Lee, Su Jung;Shin, Hye-Sun;Kang, Sung Eun;Chung, Yun Mi;Lee, Jung Mi;Jung, Yong-hyun;Moon, Guiim
Korean Journal of Environmental Agriculture
/
v.41
no.2
/
pp.82-94
/
2022
BACKGROUND: Spiropidion and its metabolite are tetramic acid insecticide and require the establishment of an official analysis method for the safety management because they are newly registered in Korea. Therefore, this study was to determine the analysis method of residual spiropidion and its metabolite for the five representative agricultural products. METHODS AND RESULTS: Three QuEChERS methods (original, AOAC, and EN method) were applied to optimize the extraction method, and the EN method was finally selected by comparing the recovery test and matrix effect results. Various adsorbent agents were applied to establish the clean up method. As a result, the recovery of spiropidion was reduced when using the dispersive-SPE method with MgSO4, primary secondary amine (PSA), graphitized carbon black (GCB) and octadecyl (C18) in soybean. Color interference was minimized by selecting the case including GCB and C18 in addition to MgSO4. This method was established as the final analysis method. LC-MS/MS was used for the analysis by considering the selectivity and sensitivity of the target pesticide and the analysis was performed in MRM mode. The results of the recovery test using the established analysis method and inter laboratory validation showed a valid range of 79.4-108.4%, with relative standard deviation and coefficient of variation were less than 7.2% and 14.4%, respectively. CONCLUSION(S): Spiropidion and its metabolite could be analyzed with a modified QuEChERS method, and the established method would be widely available to ensure the safety of residual insecticides in Korea.
A simple and precise method of $^{14}C$ was developed to analyze $^{14}C$ in the environment samples using a commercially available $^{14}CO_2$ absorbent and a liquid scintillation counter. An air sampler and a combustion system were developed to collect HTO and $^{14}CO_2$ in the air and the biological samples simultaneously. The collection yield of $^{14}CO_2$ by the air sampler was in the range of 73-89% . The yield of the combustion system was 97%. In preparing samples for counting, the optimum ratio of $CO_2$ absorbent to the scintillator for mixing was 1:1. No variation of the specific activity of $^{14}C$ in the counting sample was observed up to 70 days after preparation of the samples. The detection limit for$^{14}C$ was 0.025 Bq/gC, which is the level applicable to the natural level of $^{14}C$. The analytical result of $^{14}C$ obtained by the present method were within ${\pm}6%$ of the relative error from the one by the benzene synthesis. The specific activity of $^{14}C$ in the air collected at Taejon during the period of October 1996 ranged from 0.26 to 0.27 Bq/gC. The specific activity of $^{14}C$ in the air collected at 1km from the Wolsong nuclear power plant a 679 MWe PHWR, was $0.54{\pm}0.03$ Bq/gC. The ranges of specific activities of $^{14}C$ in the pine needles and the vegetations from the areas around the Wolsong nuclear power plant were 0.56-0.67 Bq/gC and 0.23-1.41 Bq/gC, respectively.
Journal of the Korean Society of Groundwater Environment
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v.6
no.4
/
pp.194-205
/
1999
Hydrogeochemical variation and environmental isotope at the some abandoned metal mine (Sanggok, Keumsil, Jangpung and Samdeok) creeks of the Hwanggangri mining district were carried out based upon the physicochemical properties for surface water collected of February in 1998. Hydrogeochemical composition of the all water samples are characterized by the relatively significant enrichment of Ca$^{2}$, alkaline ions, N $O_3$$^{-}$ and Cl$^{-}$ in normal surface water, whereas the surface waters near the mining area are relatively enriched in Ca$^{2+$, Mg$^{2+}$, heavy metals. HC $O_3$$^{-}$ and S $O_4$$^{2-}$. Surface waters of the mining creek have low pH, high EC and extremely high concentrations of TDS compared with surface water of the non-mining creeks. The range of $\delta$D and $\delta$$^{18}$O values (SMOW) in the waters are shown in -65.0 to-71.2$\textperthousand$ and -9.1 to-10.2$\textperthousand$. The d($\delta$D-$\delta$$^{18}$O) value with those of water samples ranged from 7.3 to 10.9. These $\delta$D and $\delta$$^{18/}$ of the acid mine water are more heavy values than those of surface water. The values have revealed the positive correlation between isotopic compositions and major elements, because those $\delta$D and $\delta$$^{18}$O values increase with increasing TDS. HC $O_3$$^{-}$ , S $O_4$$^{2-}$ and Ca$^{2+}$ concentration. Using WATEQ4F, saturation index of albite calcite, dolomite and mostly clay minerals in water of the mining area show undersaturated and progressively evolved toward the equilibrium condition due to fresh water mixing, however, surface waters of the non-mining area are nearly saturated and/or supersaturated. Geochemical modeling showed that mostly toxic heavy metals within water in the mining creek may exist largely in the from of metal-sulfate (MS $O_4$$^{2-}$), free metal (M$^{2+}$/), C $O_3$$^{-}$ and/or OH$^{-}$ complex ions. Based on the geology, water chemistry and environmental istopic data the water compositions from the Sanggok and Keumsil mine creek (consist mainly of Cambro-Ordovician carbonate rocks of the Cho-seon Supergroup) show higher PH, Ca$^{2+}$, Mg$^{2+}$ , HC $O_3$$^{-}$ and more heavy $\delta$D and $\delta$$^{18}$O values than those from the Jangpung and Samdeok mine creek (consist of age -unknown metasedimentary rocks of the Ogcheon Supergroup and/or Jurassic grani-toids), but each of these waters represents a similar hydrogeochemical evolution path by the mine water mixing.
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