• 제목/요약/키워드: Variation Part

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Studies on the Nitrogen Metabolism of Soybeans -III. Variation of Glutamic acid, Aspartic acid and its Amides during the Growth of Yonger Plants (대두(大豆)의 질소대사(窒素代謝)에 관(關)한 연구(硏究) -III. 유식물(幼植物) 시기(時期)에서의 Glutamine 산(酸)과 Asparagine 산(酸) 및 그 Amide의 소장(消長))

  • Kang, Y.H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.3 no.1
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    • pp.55-59
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    • 1970
  • In an effort to determine the bio-synthesis in the soybean as investigate to the variance of each substance: Glutamic acid, Aspartic acid and its amides during the growth of younger soybean plants. 1. The variance-curve of Gultamic acid and Aspartic acid as the acidic amino acids in the cotyledons was appeared the peak the first half period at Glutamic acid and the latter half at Aspartic acid in the growth of soybeans, and was received the symmetrical impression centering around the stage of adult leaf-development. But, in the embryonic organ, it appears the peak at both part, in the developmental stage of adult leaf and also appears near phenomena of increase and decrease in the variation-curve of metabolites. 2. It's amides-Gultamine and Asparagine-appears the peak at the developmental stage of adult leaf in the both cotyledons and embryonic organ, and rapid increase in the cotyledons were very impressed compare with the decrease at fallen stage of cotyledons in the embryonic organs. 3. In the relation of variance at Glutamic acid and Aspartic acid, both substance were discovered the fact of translocation from cotyledon to embryonic organ, and Glutamic acid could supposed that bear the charges of outrider substance in other amino acid as the Glutamic acid-self and major basic function for receiving the ammonia as the nitrogen contain constituent of plant. In the case of Glutamine, formation-mechanism of ammonia which develops due to its hydrolysis in the latter period of soybean growth, suggested that was forfeit its function till instance of fallen cotyledons. 4. In the relation the Aspartie acid and Asparagine, Aspartic acid which begins to decrease from seed-state was supposed that bear sufficiently the charge of outrider substance in the formation of Asparagine other than translocated to embryonic organ from cotyledon. And, formation-theory of Aspartic acid which suppose as formational substance from Kreb's cycle were recognized from latter period of soybean growth, and then, rapid accumulation of Asparagine's amounts were supposed that adapt to two theory: Theory which consider to transformation as Asparagine state for pressing to less than noxious weight the concentration of ammonia developing from the cells, and was formate and accumulate as ammonia or carbohydrates containing excess in the cotyledons.

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A Stochastic Study for the Emergency Treatment of Carbon Monoxide Poisoning in Korea (일산화탄소중독(一酸化炭素中毒)의 진료대책(診療對策) 수립(樹立)을 위한 추계학적(推計學的) 연구(硏究))

  • Kim, Yong-Ik;Yun, Dork-Ro;Shin, Young-Soo
    • Journal of Preventive Medicine and Public Health
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    • v.16 no.1
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    • pp.135-152
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    • 1983
  • Emergency medical service is an important part of the health care delivery system, and the optimal allocation of resources and their efficient utilization are essentially demanded. Since these conditions are the prerequisite to prompt treatment which, in turn, will be crucial for life saving and in reducing the undesirable sequelae of the event. This study, taking the hyperbaric chamber for carbon monoxide poisoning as an example, is to develop a stochastic approach for solving the problems of optimal allocation of such emergency medical facility in Korea. The hyperbaric chamber, in Korea, is used almost exclusively for the treatment of acute carbon monoxide poisoning, most of which occur at home, since the coal briquette is used as domestic fuel by 69.6 per cent of the Korean population. The annual incidence rate of the comatous and fatal carbon monoxide poisoning is estimated at 45.5 per 10,000 of coal briquette-using population. It offers a serious public health problem and occupies a large portion of the emergency outpatients, especially in the winter season. The requirement of hyperbaric chambers can be calculated by setting the level of the annual queueing rate, which is here defined as the proportion of the annual number of the queued patients among the annual number of the total patients. The rate is determined by the size of the coal briquette-using population which generate a certain number of carbon monoxide poisoning patients in terms of the annual incidence rate, and the number of hyperbaric chambers per hospital to which the patients are sent, assuming that there is no referral of the patients among hospitals. The queueing occurs due to the conflicting events of the 'arrival' of the patients and the 'service' of the hyperbaric chambers. Here, we can assume that the length of the service time of hyperbaric chambers is fixed at sixty minutes, and the service discipline is based on 'first come, first served'. The arrival pattern of the carbon monoxide poisoning is relatively unique, because it usually occurs while the people are in bed. Diurnal variation of the carbon monoxide poisoning can hardly be formulated mathematically, so empirical cumulative distribution of the probability of the hourly arrival of the patients was used for Monte Carlo simulation to calculate the probability of queueing by the number of the patients per day, for the cases of one, two or three hyperbaric chambers assumed to be available per hospital. Incidence of the carbon monoxide poisoning also has strong seasonal variation, because of the four distinctive seasons in Korea. So the number of the patients per day could not be assumed to be distributed according to the Poisson distribution. Testing the fitness of various distributions of rare event, it turned out to be that the daily distribution of the carbon monoxide poisoning fits well to the Polya-Eggenberger distribution. With this model, we could forecast the number of the poisonings per day by the size of the coal-briquette using population. By combining the probability of queueing by the number of patients per day, and the probability of the number of patients per day in a year, we can estimate the number of the queued patients and the number of the patients in a year by the number of hyperbaric chamber per hospital and by the size of coal briquette-using population. Setting 5 per cent as the annual queueing rate, the required number of hyperbaric chambers was calculated for each province and for the whole country, in the cases of 25, 50, 75 and 100 per cent of the treatment rate which stand for the rate of the patients treated by hyperbaric chamber among the patients who are to be treated. Findings of the study were as follows. 1. Probability of the number of patients per day follows Polya-Eggenberger distribution. $$P(X=\gamma)=\frac{\Pi\limits_{k=1}^\gamma[m+(K-1)\times10.86]}{\gamma!}\times11.86^{-{(\frac{m}{10.86}+\gamma)}}$$ when$${\gamma}=1,2,...,n$$$$P(X=0)=11.86^{-(m/10.86)}$$ when $${\gamma}=0$$ Hourly arrival pattern of the patients turned out to be bimodal, the large peak was observed in $7 : 00{\sim}8 : 00$ a.m., and the small peak in $11 : 00{\sim}12 : 00$ p.m. 2. In the cases of only one or two hyperbaric chambers installed per hospital, the annual queueing rate will be at the level of more than 5 per cent. Only in case of three chambers, however, the rate will reach 5 per cent when the average number of the patients per day is 0.481. 3. According to the results above, a hospital equipped with three hyperbaric chambers will be able to serve 166,485, 83,242, 55,495 and 41,620 of population, when the treatmet rate are 25, 50, 75 and 100 per cent. 4. The required number of hyperbaric chambers are estimated at 483, 963, 1,441 and 1,923 when the treatment rate are taken as 25, 50, 75 and 100 per cent. Therefore, the shortage are respectively turned out to be 312, 791. 1,270 and 1,752. The author believes that the methodology developed in this study will also be applicable to the problems of resource allocation for the other kinds of the emergency medical facilities.

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Time-series Variation of Sea Surface Salinity in the Southwestern East Sea (동해 남서부 해역 표층염분의 시계열 변동)

  • Jeong, Hee-Dong;Kim, Sang-Woo;Lim, Jin-Wook;Choi, Yong-Kyu;Park, Jong-Hwa
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.18 no.4
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    • pp.163-177
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    • 2013
  • An instrumented ferry made two transects per day across two current systems which are the North Korean Cold Current and the East Korean Warm Current over the years 2012-2013 from Gangneung to Ulleungdo in the southwestern East Sea. Seawater properties of these transects were measured with high spatial and temporal resolution for an extended period of time. Here the salinity records from the transects with the oceanographic observation data from East Sea Fisheries Institute of NFRDI, AVISO daily current chart and GOCI Chlorophyll-a image in 2012 and 2013 are used to study the time-series variation of salinity at the surface. The high salinity section with the range of 33.15~34.12 occurred on the transect mainly in the middle of eddy, and western boundary of strong northward current from June to October. We can found low salinity waters in both sides of the high salinity section. It is estimated that the western low salinity waters with the range of 30.58~33.20 accompanied by southward current were derived from the NKCC and the eastern waters with the range of 31.30~33.24 accompanied by northward current were derived from the Tsushima Surface Water. The lowest salinity of NKCC is confirmed in this study as 30.36. It is found that the western waters below 33.00 extended extremely toward the east about 110 km area from Gangneung and toward the south around Jukbyon coastal area as a 5~10 m layer. We can find its volume of low saline waters transport is not neglectable compared with that of Tsushima Current region in the western part of the East Sea. In this study we named it as the North Korean Low Saline Surface Water in summer.

Non-astronomical Tides and Monthly Mean Sea Level Variations due to Differing Hydrographic Conditions and Atmospheric Pressure along the Korean Coast from 1999 to 2017 (한국 연안에서 1999년부터 2017년까지 해수물성과 대기압 변화에 따른 계절 비천문조와 월평균 해수면 변화)

  • BYUN, DO-SEONG;CHOI, BYOUNG-JU;KIM, HYOWON
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.26 no.1
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    • pp.11-36
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    • 2021
  • The solar annual (Sa) and semiannual (Ssa) tides account for much of the non-uniform annual and seasonal variability observed in sea levels. These non-equilibrium tides depend on atmospheric variations, forced by changes in the Sun's distance and declination, as well as on hydrographic conditions. Here we employ tidal harmonic analyses to calculate Sa and Ssa harmonic constants for 21 Korean coastal tidal stations (TS), operated by the Korea Hydrographic and Oceanographic Agency. We used 19 year-long (1999 to 2017) 1 hr-interval sea level records from each site, and used two conventional harmonic analysis (HA) programs (Task2K and UTide). The stability of Sa harmonic constants was estimated with respect to starting date and record length of the data, and we examined the spatial distribution of the calculated Sa and Ssa harmonic constants. HA was performed on Incheon TS (ITS) records using 369-day subsets; the first start date was January 1, 1999, the subsequent data subset starting 24 hours later, and so on up until the final start date was December 27, 2017. Variations in the Sa constants produced by the two HA packages had similar magnitudes and start date sensitivity. Results from the two HA packages had a large difference in phase lag (about 78°) but relatively small amplitude (<1 cm) difference. The phase lag difference occurred in large part since Task2K excludes the perihelion astronomical variable. Sensitivity of the ITS Sa constants to data record length (i.e., 1, 2, 3, 5, 9, and 19 years) was also tested to determine the data length needed to yield stable Sa results. HA results revealed that 5 to 9 year sea level records could estimate Sa harmonic constants with relatively small error, while the best results are produced using 19 year-long records. As noted earlier, Sa amplitudes vary with regional hydrographic and atmospheric conditions. Sa amplitudes at the twenty one TS ranged from 15.0 to 18.6 cm, 10.7 to 17.5 cm, and 10.5 to 13.0 cm, along the west coast, south coast including Jejudo, and east coast including Ulleungdo, respectively. Except at Ulleungdo, it was found that the Ssa constituent contributes to produce asymmetric seasonal sea level variation and it delays (hastens) the highest (lowest) sea levels. Comparisons between monthly mean, air-pressure adjusted, and steric sea level variations revealed that year-to-year and asymmetric seasonal variations in sea levels were largely produced by steric sea level variation and inverted barometer effect.

Seasonal Variation in Species Composition and Abundance of Shallow Water Fishes at Taean Beaches, in the Yellow Sea of Korea (태안 해빈 천해 어류 종조성의 계절 변화)

  • Noh, Hyung-Soo;Youk, Kwan-Su;Hwang, Hak-Bin;Lee, Tae-Won
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.14 no.3
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    • pp.145-154
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    • 2009
  • Seasonal variation in species composition and abundance of shallow water fish from the Hakampo and Yeonpo beaches in Taean in the western coast of Korea were determined by the analysis of monthly samples collected by a beach seine from January to December, 2007. A total of 30 species, 964 individuals and 10,564.1 g of fish were collected from the Hakampo beach, and a total of 46 species, 4,447 individuals and 28,622.4 g of fish from the Yeonpo beach. The juveniles of coastal fish such as Chelon haematochelius, Paralichthys olivaceus, Repomucenus lunatus, Sebastes schlegelii and Takifugu niphobles were predominated in abundance. And the juveniles of pelagic migrants such as Konosirus punctatus, Sardinella zunasi and Engraulis japonicus were abundantly collected between summer and autumn. The fish collected were mainly composed of small-sized species and juveniles. C. haematochelius and migrant fish were young of the year, and commercially important fish such as S. schlegeli, P. olivaceus, Pleuronectes yokohamae and Hexagrammos otakii were 1 to 2 years old juveniles. It is considered that they use the shallow water as a nursery ground until they move out to the deeper water. The number of species and abundance were lower in the fine sand Hakamp beach than in the muddy sand Yeonpo beach where some Zostera marina were also found. In Yeonpo beach the adult of Gymnogobius mororanus preferred to live in the muddy shallow water and Syngnathus schlegeli living in the sea grass were also abundantly collected in spring in addition to resident fish and pelagic migrants in warm months. The resident species were more abundance in the Taean beach than in the beach located in the southern part of the west coast of Korea where the juveniles of pelagic migrants were more abundant.

Seasonal Variations of Water Quality in the Lower Part of the Nagdong River (낙동강 하류수질의 계절적 변화)

  • KIM Yong-Gwan;SHIM Hye-Kung;CHO Hak-Rae;YOU Sun-Jae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.6
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    • pp.511-522
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    • 1984
  • The Nagdong is one of the biggest rivers in Korea, which is very important water source not only for tap water of Pusan city but also for the industrial water. Therefore, authors tried to check the water quality year by year. In this experiment one hundred and twenty water samples collected from August 1983 to July 1984 were analyzed bacteriologically and physiologically. Fifteen sampling stations were established between near Samrangjin and estuary of the river. To evaluate the water quality, temperature, pH, chloride ion, salinity, chemical oxygen demand (COD), electrical conductivity, nutrients, total coliform, fecal coliform, fecal streptococcus, viable cell count and bacterial flora were observed. The variation of water temperature was ranged $-1.5{\sim}29.0^{\circ}C$ (Mean value $13.9{\sim}16.5^{\circ}C$), it in spring was higher as $10{\sim}15^{\circ}C$ about $10^{\circ}C$ than in winter and it in autumm was very stabilized as about $20^{\circ}C$ at each station. The pH variation of the samples was ranged $6.68{\sim}9.15$. The range of concentration of chloride ion and salinity varied $7.4{\sim}l,020.5$ mg/l and $1.05{\sim}33.0\%0$, respectively. Especially, salinity of the 3rd water war was the higher than others as $25.76{\sim}31.58\%0$. COD was ranged $1.45{\sim}14.94$ mg/l and the lower part of the Nagdong River was heavily contaminated by domesitc sewage and waste water from the adjacent factor area. The range of electrical conductivity was $1.360{\times}10^2{\sim}5.650{\times}10^4{\mu}{\mho}/cm$ and that was by far higher the estuary than the upper. Concentration of nutrients were $0.008{\sim}0.040$ mg/l (Mean value $0.019{\sim}0.068$ mg/l) for $NO_2-N,\;0.038{\sim}5.253$ mg/l ($0.351{\sim}2.347$ mg/l) for $NO_3-N,\;0.100{\sim}2.685$ mg/l($0.117{\sim}1.380$ mg/l) for $NH_4-N,\;0.003{\sim}0.084$ mg/l($0.014{\sim}0.065$ mg/l) for $PO_4-P$ and $0.154{\sim}6.123$ mg/l ($1.165{\sim}3.972$ mg/l) for $SiO_2-Si$, respectively. Usually nutrients contents of the water in the upper part(included station 1 to 5) were higher than those of the estuarine area. The bacterial density of the samples ranged 7.3 to 460,000/100 ml for total coliforms, 3.6 to 460,000/100 ml for fecal coliform, $0{\sim}46,000/100ml$ for fecal streptococcus and $<30{\sim}1.2{\times}10^5/ml$ for viable cell count. Composition of coliform was $28\%$ Escherichia coli group, $18\%$ Citrobacter freundii group, $31\%$ Enterobacter aerogenes group and $22\%$ others. Predominant species among the 659 strains isolated from the samples were Pseudomonas spp. ($42\%$), Flavobacterium spp. ($20\%$) and Moraxella spp. ($12\%$).

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Last Glacial Maximum-Holocene Variability in Geochemical Records of a Core Sediment from the Southern Part of the Ulleung Basin, East Sea: Implications for Paleoceanographic Changes (동해 울릉분지 남단 주상퇴적물에 대한 최종빙기-홀로세간의 지화학적 기록 변화: 고해양환경 변화)

  • Huh, Sik;Han, Sang-Joon;Hyun, Sang-Min
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.6 no.2
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    • pp.71-80
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    • 2001
  • To understand paleoceanographic environmental changes in the Esat Sea during the transitional period between Holocene and last glacial maximum, geochemical high resolution study was conducted by using a piston core(95PC-1) samples collected from the southernmost part of the Ulleung Basin. Geochemical results reveal that major distinctive paleoceanographic variations in transitional period are prominent. Major elemental concentrations show distinctive variations between glacial and Holocene suggesting changes in sediment supply. $TiO_2/Al_2O_3$ ratio of the sediment indicates different sediment composition between Holocene and glacial period. The content of total organic carbon ranging from 0.5% to 4% during transitional period. These vslues showed 2-4 times and two times higher than those of last glacial and Holocene, respectively. The C/N ratios deduced from organic matters exceed10 during transitional period suggesting terrigenous organic matter are supplied from continent, especially during last glacial maximum. Carbonate contents are relatively stable during Holocene and last glacial maximum with gradual decrease during glacial period with high fluctuation during transitional period. The variations of chemical index of weathering (CIW) also show a distinctive variation between glacial and Holocene, which is coincident with those of carbonate and organic carbon. The grain size distribution indicates that the difference content of silt fraction during Holocene and glacial period is closely related with climatic effect during glacial period. Therefore geochemical differences in sediment composition between Holocene and last glacial maximum is thought to be related to paleoceanographic, sea-level change and local paleoclimatic changes.

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Characteristics of Flow and Sedimentation around the Embankment (방조제 부근에서의 흐름과 퇴적환경의 특성)

  • Lee Moon Ock;Park Il Heum;Lee Yeon Gyu
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.3 no.4
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    • pp.37-55
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    • 2000
  • Two-dimensional numerical experiments and field surveys have been conducted to clarify some environmental variations in the flow and sedimentation in the adjacent seas after the construction of a tidal embankment. Velocities of flow and water levels in the bay decreased after the construction of the barrage. When the freshwater was instantly released into the bay, the conditions of flow were unaltered, with the exception of a minor variation in velocities and tidal levels around the sluices at the ebb flow. The computational results showed that freshwater released at the low water reached the outside of the bay and then returned to the inside with the tidal currents at the high water. The front sea regions of the embankment had a variety of sedimentary phases such as a clayish silt, a silty clay and a sandy clayish silt. However, a clayish silt was prevalent in the middle of the bay. On the other hand, the skewness, which reflects the behaviour of sediments, was $\{pm}0.1$ at the front regions of the embankment while it was more than ±0.3 in the middle of the bay. Analytical results of drilling samples acquired from the front of the sluice gates showed that the lower part of the sediments consists of very fine silty or clayish grains. The upper surface layer consisted of shellfish, such as oyster or barnacle with a thickness of 40~50 cm. Therefore, it seemed that the lower part of the sediments would have been one of intertidal zones prior to the embankment construction while the upper shellfish layer would have been debris of shellfish farms formed in the adjacent seas after the construction of the embankment. This shows the difference of sedimentary phases reflected the influence of a tidal embankment construction.

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Seasonal distribution and primary production of microphytobenthos on an intertidal mud flat of the Janghwa in Ganghwa Island, Korea (강화도 장화리 갯벌에서 저서미세조류의 계절적 분포 및 일차 생산력)

  • Yoo, Man-Ho;Choi, Joong-Ki
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.10 no.1
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    • pp.8-18
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    • 2005
  • We studied seasonal distribution of the microphytobenthos and their primary production with $C^{14}$ method and carried out pigment analysis with HPLC in an estuarine mudflat of the Ganghwa Island, Korea from May 2002 to April 2004. The abundances of microphytobenthos were higher at the middle than upper part and lower part of intertidal flat. Abundances of microphytobenthos ranged from $2.3{\times}10^5\;cells\;cm^{-2}$ to $140.9{\times}10^5\;cells cm^{-2}$. The bloom of microphytobenthos was observed in the early spring and then it decreased from spring to summer and autumn. The pennate diatom was a predominated group among the microphytobenthos in this area. The dominant species were Paralia sulcata, Cylindrotheca closterium and Nitzschia sp.. Nitzschia sp. and Cylindrotheca closterium were predominant in February. The results of pigment analysis suggest the presence of diatoms, euglenophytes, chlorophytes, cyanobacteria, cryptophytes, chrysophytes, prymnesiophytes, dinoflagellates and prasinophytes. The biomass of microphytobenthos ranged from 1.18 to 34.25 mg chl-a $m^{-2}$, with a mean of 7.60 mg chl-a $m^{-2}$. The mean ratio of Fuco/Chl a was 0.7 which indicates that most of biomasses of microphytobenthos were due to diatoms. The ratios of Chl b/Chl a ranged from 0 to 0.82(with a mean of 0.17), implying that euglenophytes and chlorophytes lived together in special period seasonally. Temporal variation of primary production ranged from 4.2 to 113.0 $mgC{\cdot}m^{-2}{\cdot}hr^{-1}$(mean value was 33.9 $mgC{\cdot}m^{-2}{\cdot}hr^{-1}$ and initial slope$({\alpha})$ was measured from 0.002-0.005$(mgC\;mgchl-a^{-1}\;hr^{-1}){\cdot}({\mu}E\;m^{-2}\;s^{-1})^{-1}$. Assimilation number$(P_m)$ was in the range of 0.50-1.32 $mgC{\cdot}mgChl-a{\cdot}hr^{-1}$ and daily primary production ranged from 20.9 to 678.1 $mgC{\cdot}m^{-2}{\cdot}d^{-1}$(mean value was 206.72 $mgC{\cdot}m^{-2}{\cdot}^{-1}$).

A New Method For Measuring Acupoint Pigmentation After Cupping Using Cross Polarization (교차편광 촬영술(Cross Polarization Photographic Technique)를 이용한 부항요법의 배수혈 피부 색소 침착 변화 측정 평가)

  • Kim, Soo-Byeong;Jung, Byungjo;Shin, Tae-Min;Lee, Yong-Heum
    • Korean Journal of Acupuncture
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    • v.30 no.4
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    • pp.252-263
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    • 2013
  • Objectives : Skin color deformation by cupping has been widely used as a diagnostic parameter in Traditional Korean Medicine(TKM). Skin color deformation such as ecchymoses and purpura is induced by local vacuum in a suction cup. Since existing studies have relied on a visual diagnostic method, there is a need to use the quantitative measurement method. Methods : We conducted an analysis of cross-polarization photographic images to assess the changes in skin color deformation. The skin color variation was analyzed using $L^*a^*b^*$ space and the skin erythema index(E.I.). The meridian theory in TKM indicates that the condition of primary internal organs is closely related to the skin color deformation at special acupoints. Before conducting these studies, it is necessary to evaluate whether or not skin color deformation is influenced by muscle condition. Hence, we applied cupping at BL13, BL15, BL18, BL20 and BL23 at Bladder Meridian(BL) and measured blood lactate at every acupoint. Results : We confirmed the high system measurement accuracy, and observed the diverse skin color deformations. Moreover, we confirmed that the $L^*$, $a^*$ and E.I. had not changed after 40 minutes(p>0.05). The distribution of blood lactate levels at each part was observed differently. Blood lactate level and skin color deformation at each part was independent of each other. Conclusions : The negative pressure produced by the suction cup induces a reduction in the volumetric fraction of melanosomes and subsequent reduction in epidermal thickness. The relationship between variations of tissue and skin properties and skin color deformation degree must be investigated prior to considering the relationship between internal organ dysfunction and skin color deformation.