• Title/Summary/Keyword: Validation Method

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Determination of Aflatoxins in Nuts, Their Products and Dried Fruits Using Rapid Resolution Liquid Chromatography Coupled with Tandem Mass Spectrometry (RRLC-MS/MS를 이용한 견과류 및 그 가공품과 건조과실류의 아플라톡신 분석)

  • Choi, Su-Jeong;Park, Ju-Sung;Jung, So-Young;Son, Yeo-Joon;Lyi, Yun-Jeong;Kim, Mi-Sun;Park, So-Hyun;Lee, Sang-Me;Chae, Young-Zoo
    • Korean Journal of Food Science and Technology
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    • v.43 no.6
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    • pp.702-710
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    • 2011
  • The occurrence of aflatoxins $B_1$, $B_2$, $G_1$ and $G_2$ in nuts, their products and dried fruits was investigated. Samples were collected from local markets in Seoul and analyzed by rapid resolution liquid chromatography coupled with tandem mass spectrometry using an immunoaffinity column. The chromatography method was validated for assay of aflatoxins using linearity, accuracy, precision and limit of detection and quantification. The linearity in the concentration ranged from 0.10 to $20{\mu}g/kg$ with $R^2$ > 0.9999. Sample recoveries ranged from 71.1 to 97.2% with relative standard deviations below 4.5% for spiking levels from 1 to $10{\mu}g/kg$. The limits of detection ranged between 0.02 and $0.05{\mu}g/kg$ and the limits of quantification ranged between 0.05 and $0.10{\mu}g/kg$. The levels of aflatoxin $B_1$, $B_2$, $G_1$ and $G_2$ in nuts, their products and dried fruits were $B_1$ 0.10 to $9.94{\mu}g/kg$, $B_2$ 0.08 to $1.54{\mu}g/kg$, $G_1$ 0.04 to $3.21{\mu}g/kg$ and $G_2$ 0.06 to $0.14{\mu}g/kg$.

Regional Identity and Community Paper: A Search for Subject and Method of Geographical Research (지역정체성 연구와 지역신문의 활용 -지리학적 연구주제의 탐색-)

  • Lee, Young-Min
    • Journal of the Korean association of regional geographers
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    • v.5 no.2
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    • pp.1-14
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    • 1999
  • In the course of modernization and globalization, each region in Korea has experienced deep subordination to the center of Seoul and the increase of colonization possibility by world capital. In order to overcome the current situation, above all, the strategies should be developed focusing on daily life and life space. The basis for the development of strategies is the establishment of regional identity on life space. It is because of the reason that life space, or small-scale region has drawn wide attention in the research of geography in recent years. Especially, humanistic geography and new regional geography have developed the concerning theory and methodology, and kept going on the research of small-scale regions. Generally speaking, there have been quite large amount of theoretical discussions on small-scale region in recent years in the field of geography. Empirical researches focusing on a particular small-scale region, however, have been rarely made. It seems related to the deficiency of data materials and the obscurity of research framework of small-scale regional geography. A community paper must be very helpful for the geographic research on small-scale region. As community paper is published based on county('gun'), small or mid-size city('si'), or district of large city('gu'), it deals with small news and daily life information closely attached to the region. Accordingly, it functions as a medium of the formation of regional identity. It is also a valuable source material for the validation of regional identity and for the analysis of identity-shaping mechanism. The geographic interests in community paper, first of all, should be taken shape by the work on the geographical distribution and the periodic change of publication situation of community papers in Korea. Another research subject on community paper is the examination of characteristics of the region by analyzing the news and the advertisements. The news in community paper must be a valuable data source of regional studies in geography. Also, the regional identification process of community people through the community paper could be and should be explored, and how the regional centrality, or self-generation based on the identity is achieved will be an important subject.

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Development of Near-Infrared Reflectance Spectroscopy (NIRS) Model for Amylose and Crude Protein Contents Analysis in Rice Germplasm (근적외선 분광광도계를 이용한 벼 유전자원 아밀로스 및 단백질 함량분석을 위한 모델개발)

  • Oh, Sejong;Lee, Myung Chul;Choi, Yu Mi;Lee, Sukyeung;Oh, Myeongwon;Ali, Asjad;Chae, Byungsoo;Hyun, Do Yoon
    • Korean Journal of Plant Resources
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    • v.30 no.1
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    • pp.38-49
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    • 2017
  • The objective of this research was to develop Near-Infrared Reflectance Spectroscopy (NIRS) model for amylose and protein contents analysis of large accessions of rice germplasm. A total of 511 accessions of rice germplasm were obtained from National Agrobiodiversity Center to make calibration equation. The accessions were measured by NIRS for both brown and milled brown rice which was additionally assayed by iodine and Kjeldahl method for amylose and crude protein contents. The range of amylose and protein content in milled brown rice were 6.15-32.25% and 4.72-14.81%, respectively. The correlation coefficient ($R^2$), standard error of calibration (SEC) and slope of brown rice were 0.906, 1.741, 0.995 in amylose and 0.941, 0.276, 1.011 in protein, respectively, whereas $R^2$, SEC and slope of milled brown rice values were 0.956, 1.159, 1.001 in amylose and 0.982, 0.164, 1.003 in protein, respectively. Validation results of this NIRS equation showed a high coefficient determination in prediction for amylose (0.962) and protein (0.986), and also low standard error in prediction (SEP) for amylose (2.349) and protein (0.415). These results suggest that NIRS equation model should be practically applied for determination of amylose and crude protein contents in large accessions of rice germplasm.

Development of Prediction Equation of Diffusing Capacity of Lung for Koreans

  • Hwang, Yong Il;Park, Yong Bum;Yoon, Hyoung Kyu;Lim, Seong Yong;Kim, Tae-Hyung;Park, Joo Hun;Lee, Won-Yeon;Park, Seong Ju;Lee, Sei Won;Kim, Woo Jin;Kim, Ki Uk;Shin, Kyeong Cheol;Kim, Do Jin;Kim, Hui Jung;Kim, Tae-Eun;Yoo, Kwang Ha;Shim, Jae Jeong
    • Tuberculosis and Respiratory Diseases
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    • v.81 no.1
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    • pp.42-48
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    • 2018
  • Background: The diffusing capacity of the lung is influenced by multiple factors such as age, sex, height, weight, ethnicity and smoking status. Although a prediction equation for the diffusing capacity of Korea was proposed in the mid-1980s, this equation is not used currently. The aim of this study was to develop a new prediction equation for the diffusing capacity for Koreans. Methods: Using the data of the Korean National Health and Nutrition Examination Survey, a total of 140 nonsmokers with normal chest X-rays were enrolled in this study. Results: Using linear regression analysis, a new predicting equation for diffusing capacity was developed. For men, the following new equations were developed: carbon monoxide diffusing capacity (DLco)=-10.4433-0.1434${\times}$age (year)+0.2482${\times}$heights (cm); DLco/alveolar volume (VA)=6.01507-0.02374${\times}$age (year)-0.00233${\times}$heights (cm). For women the prediction equations were described as followed: DLco=-12.8895-0.0532${\times}$age (year)+0.2145${\times}$heights (cm) and DLco/VA=7.69516-0.02219${\times}$age (year)-0.01377${\times}$heights (cm). All equations were internally validated by k-fold cross validation method. Conclusion: In this study, we developed new prediction equations for the diffusing capacity of the lungs of Koreans. A further study is needed to validate the new predicting equation for diffusing capacity.

Development of the Risk Evaluation Model for Rear End Collision on the Basis of Microscopic Driving Behaviors (미시적 주행행태를 반영한 후미추돌위험 평가모형 개발)

  • Chung, Sung-Bong;Song, Ki-Han;Park, Chang-Ho;Chon, Kyung-Soo;Kho, Seung-Young
    • Journal of Korean Society of Transportation
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    • v.22 no.6
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    • pp.133-144
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    • 2004
  • A model and a measure which can evaluate the risk of rear end collision are developed. Most traffic accidents involve multiple causes such as the human factor, the vehicle factor, and the highway element at any given time. Thus, these factors should be considered in analyzing the risk of an accident and in developing safety models. Although most risky situations and accidents on the roads result from the poor response of a driver to various stimuli, many researchers have modeled the risk or accident by analyzing only the stimuli without considering the response of a driver. Hence, the reliabilities of those models turned out to be low. Thus in developing the model behaviors of a driver, such as reaction time and deceleration rate, are considered. In the past, most studies tried to analyze the relationships between a risk and an accident directly but they, due to the difficulty of finding out the directional relationships between these factors, developed a model by considering these factors, developed a model by considering indirect factors such as volume, speed, etc. However, if the relationships between risk and accidents are looked into in detail, it can be seen that they are linked by the behaviors of a driver, and depending on drivers the risk as it is on the road-vehicle system may be ignored or call drivers' attention. Therefore, an accident depends on how a driver handles risk, so that the more related risk to and accident occurrence is not the risk itself but the risk responded by a driver. Thus, in this study, the behaviors of a driver are considered in the model and to reflect these behaviors three concepts related to accidents are introduced. And safe stopping distance and accident occurrence probability were used for better understanding and for more reliable modeling of the risk. The index which can represent the risk is also developed based on measures used in evaluating noise level, and for the risk comparison between various situations, the equivalent risk level, considering the intensity and duration time, is developed by means of the weighted average. Validation is performed with field surveys on the expressway of Seoul, and the test vehicle was made to collect the traffic flow data, such as deceleration rate, speed and spacing. Based on this data, the risk by section, lane and traffic flow conditions are evaluated and compared with the accident data and traffic conditions. The evaluated risk level corresponds closely to the patterns of actual traffic conditions and counts of accident. The model and the method developed in this study can be applied to various fields, such as safety test of traffic flow, establishment of operation & management strategy for reliable traffic flow, and the safety test for the control algorithm in the advanced safety vehicles and many others.

Developing a Questionnaire to Assess Exposure to Food-Borne Hazards (식품으로 인한 유해물질 노출평가를 위한 설문지 개발)

  • Shin, Sang-Ah;Choi, Seul-Ki;Kim, Hye-Mi;Lee, Kyung-Youn;Shin, Sang-Hee;Lee, Jung-Won;Yu, Soo-Hyun;Nam, Hye-Soen;Yoon, Hae-Jung;Joung, Hyo-Jee
    • Journal of Nutrition and Health
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    • v.44 no.1
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    • pp.61-73
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    • 2011
  • The aim of this study was to develop a reliable dietary questionnaire to assess human exposure to food-borne hazards. Eleven food-borne hazards were chosen as a priority control list through a literature review and advisory committees. The 11 food-borne hazards were phthalate, aflatoxin, bisphenol A, polycyclic aromatic hydrocarbons, dioxin, polychlorinated biphenyls, mercury, lead, cadmium, arsenic, and acrylamide. The characteristics, exposure level, and paths of these hazards were reviewed, and questionnaire items were identified to assess human exposure from the literature. A questionnaire was developed for each selected food based on its characteristics. Based on the items in the individual questionnaires, a comprehensive questionnaire, which contained demographic characteristics, job information, socioeconomic factors, health related lifestyles, and dietary behaviors, was developed. A 99-item food frequency questionnaire (FFQ) to assess food-borne hazard exposure was also developed. The FFQ included frequency of food intake during the previous year, container type for purchasing and storing food, and cooking method. The questionnaire developed in this study could be applied to assess dietary factors during an exposure assessment of food-borne hazards in a large population. A validation study for the questionnaire is needed before applying it to surveys.

Investigation of Water-soluble Vitamin (B1, B2, and B3) Contents in Various Roasted, Steamed, Stir-fried, and Braised Foods Produced in Korea (국내 식품 중 구이, 찜, 볶음, 조림에 존재하는 수용성 비타민 B1, B2 그리고 B3 함량 조사)

  • Cho, Jin-Ju;Hong, Seong Jun;Boo, Chang Guk;Jeong, Yuri;Jeong, Chang Hyun;Shin, Eui-Cheol
    • Journal of Food Hygiene and Safety
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    • v.34 no.5
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    • pp.454-462
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    • 2019
  • A conventional Korean meal typically includes various roasted, steamed, stir-fried, and braised foods. For this study, we investigated the contents of water soluble vitamins, $B_1$ (thiamin), $B_2$ (riboflavin) and $B_3$ (niacin) in various roasted, steamed, stir-fried, and braised foods. Method validation for analytical data in this study showed a high linearity ($r^2$>0.999), and the limit of detection and quantification were 0.001-0.067 and $0.002-0.203{\mu}g/mL$, respectively. For accuracy and precision, analytical values using standard reference materials were in the certified ranges. Roasted foods contained 0.039-1.057 mg/100 g of thiamin, 0.058-0.686 mg/100 g of riboflavin and 0.021-21.772 mg/100 g of niacin. Steamed foods contained 0.049-1.066 mg/100 g of thiamin, 0.025-0.548 mg/100 g of riboflavin and 0.134-21.509 mg/100 g of niacin. Stir-fried foods contained 0.114-0.388 mg/100 g of thiamin, 0.014-1.258 mg/100 g of riboflavin and 0.015-2.319 mg/100 g of niacin. Braised foods contained 0.112-1.656 mg/100 g of thiamin, 0.024-0.298 mg/100 g of riboflavin and 0.322-2.157 mg/100 g of niacin. The data on water-soluble vitamins in this study can be used for a nutritional database of conventional Korean meals.

Research on the influence of web celebrity live broadcast on consumer's purchase intention - Adjusting effect of web celebrity live broadcast contextualization

  • Zou, Ji-Kai;Guo, Han-Wen;Liu, Zi-Yang
    • Journal of the Korea Society of Computer and Information
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    • v.25 no.6
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    • pp.239-250
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    • 2020
  • The purpose of this paper is to explore the influence of the "contextualization" effect of web celebrity live broadcast on the e-commerce platform on consumers' perception of product value, risk and purchase intention. Live in this paper, using Taobao shopping consumers as the research object, the survey method, questionnaire survey is adopted, the form through the questionnaire and distributed network, a live in order to further validation of web celebrity effect of contextualized actual influence on consumer purchase intention, questionnaire design the Likert scale, seven and recycling questionnaire analysis using the statistical software SPSS 23.0 and AMOS 22.0 after processing the data. After determining the reliability and validity of the questionnaire, the exploratory factor analysis was used to verify the hypothesis and calculate the actual adjustment degree of the "contextualization" effect of web celebrity live broadcasting on consumers' purchase intention. The research results of this paper are summarized as follows :(1) consumers' perceived value of products can significantly positively affect their purchase intention, while perceived risk has a significantly negative impact on their purchase intention; (2) consumers' trust and purchase intention to products are regulated by the "contextualization" of web celebrity live broadcast. Specifically, for web celebrity live broadcasting with good "contextualization" effect, the perceived value of consumer products has a positive impact on product trust, which is higher than that of web celebrity live broadcasting with poor "contextualization" effect. In terms of resolving consumers' perceived risks to products, web celebrity live broadcast with good "contextualization" effect is also significantly better than web celebrity live broadcast with poor "contextualization" effect. Based on empirical analysis, this paper concludes that web celebrity live broadcasting will become a new breakthrough for the sustainable growth of the e-commerce industry, and puts forward Suggestions on the e-commerce marketing mode and the transformation of web celebrity live broadcasting industry.

Estimation Model for Simplification and Validation of Soil Water Characteristics Curve on Volcanic Ash Soil in Subtropical Area in Korea (난지권 화산회토양의 토색별 토양수분 특성곡선 및 단일화 추정모형)

  • Hur, Seung-Oh;Moon, Kyung-Hwan;Jung, Kang-Ho;Ha, Sang-Keun;Song, Kwan-Cheol;Lim, Han-Cheol;Kim, Geong-Gyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.6
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    • pp.329-333
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    • 2006
  • Most of volcanic ash soils in South Korea are distributed in Jeju province which is an island placed on southern part of Korea and has steep slope mountain area. There are many soils containing high contents of organic matter (OM) derived from volcanic ash in Jejudo, also. Therefore, irrigation and drainage in volcanic ash soil different with general soil which has low OM content have to be applied with another management way, but studies searching appropriate methods for them are set on insufficient situation because the area of volcanic ash soil in South Korea is only 1.3% (130,000ha). This study was conducted for analysis of soil water content and irrigation quantity appropriate for crops cultivated in volcanic ash soil with high OM content. Although soils with different soil color have the same soil texture, soil water characteristics curve by soil color showed the difference of water retention capability by OM content. But, this characteristics classified with soil color could be unified by scaling technique with similitude analysis method which get dimensionless water content using a present water content, a residual water content and saturated water content (or water content at 10kPa). A relation of gravimetric soil water content (GSWC) and dimensionless water content by the results showed a form of power function. The dimensionless water content (DWC) express a relative saturation degree of present water content. This was also expressed by van Genuchten model which describe the relation between relative saturation degrees and matric potentials. These results on soil water characteristics curve (SWCC) of volcanic ash soil will be the basic of irrigation plan in area having high organic contents into soil.

A Safety Survey on Pesticide Residues in Dried Agricultural Products (건조농산물의 잔류농약 안전성 조사)

  • Lee, Hyo-Kyung;Oh, Moon-Seog;Jeong, Jin-A;Kim, Ki-Yu;Lee, Seong-Bong;Kim, Han-Taek;Kang, Hyang-Ri;Son, Ji-Hee;Lee, Yun-Mi;Lee, Mi-Kyoung;Lee, Byoung-Hoon;Kim, Ji-Won;Park, Yong-Bae
    • Journal of Food Hygiene and Safety
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    • v.34 no.4
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    • pp.340-347
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    • 2019
  • We performed a safety survey on residual pesticides in dried agricultural products. A total of 110 samples of dried agricultural products distributed in Gyeonggi-do were analyzed for 263 pesticides according to multi class pesiticide multiresidue method. Ten types of pesticides were detected in 10 samples. Chlorpyrifos was detected in Ricinus communis leaves, chlorpyrifos, hexaconazole, pyridalyl in Chwinamul (wild aster), diniconazole, isoprothiolane, lufenuron in radish leaves, hexaconazole in Cirsium setidens (Korean thistle), bifenthrin, and chlorothalonil, boscalid, and pyraclostrobin in pepper leaves. The detection rate of pesticides was 9.1%, and among these samples, one was detected over Maximum Residue Limits (MRLs). In the validation study, the values of limit of detection (LOD), limit of quantitation (LOQ), coefficient of determination ($R^2$) and recovery were in the range of 0.002~0.027 mg/kg, 0.006~0.083 mg/kg, 0.9964~1.0000 and 74.8~118.9%, respectively. The Positive List System (PLS) was newly introduced as part of the safety management of residual pesticide in agricultural products in Korea in 2019. With the application of the PLS, if the MRL is not established, 0.01 mg/kg limit is applied uniformly. In spite, these of strengthened residue limits, the MRLs of dried agricultural products are still insufficient. Therefore, this study could be utilized as basic data for the setting of proper MRLs.