• Journal of the Korean Society of Marine Environment & Safety
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• v.27 no.7
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• pp.1098-1105
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• 2021

The International Maritime Organization has steadily strengthened environmental regulations on nitrogen oxides and carbon dioxide emitted from marine vessels. Consequently, the demand for electric propulsion vessels based on eco-friendly elements has increased. To this end, research and development has been steadily conducted for various vessels. In electric propulsion systems, a redundancy configuration is typically adopted to increase reliability and facilitate the onboard arrangement. Furthermore, studies have been actively conducted to ensure the safety of electric propulsion systems through the combination with digital twin technology. A digital twin can be used to predict outcomes in advance by implementing real-world equipment or space in a virtual world like twins, integrating real-world information and data with the virtual world, and performing computer simulations of situations that can occur in a real environment. In this study, we perform failure modes and effects analysis (FMEA) to validate the electric power management system (PMS) redundancy scheme for the digital twin technology development of electric propulsion vessels. Then, we propose the role and algorithm of PMS as a compensation function for preventing primary and secondary damages caused by a single equipment failure of the PMS and preventing additional damages by analyzing the impact on the entire system under real vessel operating conditions based on the redundancy FMEA suggested for the ship classification and certification. We verified the improvement in propulsion conservation through tests.

• Journal of Environmental Health Sciences
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• v.48 no.3
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• pp.159-166
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• 2022

Background: A quantitative structure-activity relationship (QSAR) model was adopted in the Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH, EU) regulations as well as the Act on Registration, Evaluation, etc. of Chemicals (AREC, Republic of Korea). It has been previously used in the registration of chemicals. Objectives: In this study, we investigated the correlation between the predicted data provided by three prediction programs using a QSAR model and actual experimental results (acute fish, daphnia magna toxicity). Through this approach, we aimed to effectively conjecture on the performance and determine the most applicable programs when designating toxic substances through the AREC. Methods: Chemicals that had been registered and evaluated in the Toxic Chemicals Control Act (TCCA, Republic of Korea) were selected for this study. Two prediction programs developed and operated by the U.S. EPA - the Ecological Structure-Activity Relationship (ECOSAR) and Toxicity Estimation Software Tool (T.E.S.T.) models - were utilized along with the TOPKAT (Toxicity Prediction by Komputer Assisted Technology) commercial program. The applicability of these three programs was evaluated according to three parameters: accuracy, sensitivity, and specificity. Results: The prediction analysis on fish and daphnia magna in the three programs showed that the TOPKAT program had better sensitivity than the others. Conclusions: Although the predictive performance of the TOPKAT program when using a single predictive program was found to perform well in toxic substance designation, using a single program involves many restrictions. It is necessary to validate the reliability of predictions by utilizing multiple methods when applying the prediction program to the regulation of chemicals.

• Korean Journal of Culture and Social Issue
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• v.28 no.2
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• pp.133-161
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• 2022

The purpose of this study was to translate and validate the Lesbian, Gay, and Bisexual Identity Scale(LGBIS), which is a scale measuring the multidimensional identities of sexual minorities. For this, we conducted an exploratory factor analysis using a sample of 201 LGBs(homosexuals and bisexuals). Identical to the original scale, eight factors were extracted from the translated scale(i.e., concealment motivation, identity uncertainty, internalized homonegativity, difficult process, acceptance concerns, identity superiority, identity centrality, and identity affirmation), but one item was removed. In addition, the reliability coefficients of the eight factors except for concealment Motivation were satisfactory. The Confirmatory factor analysis revealed that the 8-factor model had a good fit to the data. Finally, to verify the criterion validity, correlation among each eight factor and the scales measuring the identity related scale and the psychological-functioning scale were analyzed. In conclusion, the Korean version of the LGBIS consists of 8 factors and 26 items in total. Also it was found to be a valid measure for LGBS in Korea as well.

• Korean Journal of Culture and Social Issue
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• v.27 no.3
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• pp.259-284
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• 2021

The present study was conducted to develop and validate a psychological scale that measures individuals' attitude toward unification of Korea (ATU-K). Building on major theoretical perspectives on the structure of attitude and the attitude-behavior link, we specified two sub-components each representing the cognitive and the affective dimension of people's attitude toward unification. In a survey that involved a stratified sample of Korean adults (N = 1,500), we found strong evidence showing the construct validity of the ATU-K scale. We also found evidence for the convergent and discriminant validity of the scale. In a second survey involving another stratified sample of 1,500 Korean adults, we found the utility of the ATU-K scale in predicting people's intention to engage in unification-oriented behaviors (personal/collective). We also found that the ATU-K scale fares better in predicting the intention vis-a-vis the other measures of unification-related beliefs reported in previous research. We discuss implications of our findings and directions for future research.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.35 no.5
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• pp.299-308
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• 2022

This study was conducted to predict the tendency for heat exchange and boil-off gas (BOG) in a liquefied hydrogen tank under sloshing excitation. First, athe fluid domain excited by sloshing was modeled using a multiphase-thermal flow domain in which liquid hydrogen and hydrogen gas are in the saturated state. Both the the volume of fluid (VOF) and Eulerian-based multi-phase flow methods were applied to validate the accuracy of the pressure prediction. Second, it was indirectly shown that the fluid velocity prediction could be accurate by comparing the free surface and impact pressure from the computational fluid dynamics with those from the experimental results. Thereafter, the heat ingress from the external convective heat flux was reflected on the outer surfaces of the hydrogen tank. Eulerian-based multiphase-heat flow analysis was performed for a two-dimensional Type-C cylindrical hydrogen tank under rotational sloshing motion, and an inflation technique was applied to transform the fluid domain into a computational grid model. The heat exchange and heat flux in the hydrogen liquid-gas mixture were calculated throughout the analysis,, whereas the mass transfer and vaporization models were excluded to account for the pure heat exchange between the liquid and gas in the saturated state. In addition, forced convective heat transfer by sloshing on the inner wall of the tank was not reflected so that the heat exchange in the multiphase flow of liquid and gas could only be considered. Finally, the effect of sloshing on the amount of heat exchange between liquid and gas hydrogen was discussed. Considering the heat ingress into liquid hydrogen according to the presence/absence of a sloshing excitation, the amount of heat flux and BOG were discussed for each filling ratio.

• Korean Journal of Culture and Social Issue
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• v.29 no.1
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• pp.1-23
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• 2023

The purpose of this study was to validate the Suicidal Ideation Attributes Scale(SIDAS) which can measure the severity of suicidal ideation in a sample of 399 Korean adults. For this purpose, an online survey was conducted for two weeks from July 2020 to August 2020. First, there were differences in SIDAS scores among groups divided by gender, residence status, depression and anxiety symptoms, suicidal ideation, suicidal plan, suicidal preparation, and suicidal attempt. Second, correlations between SIDAS, C-SSRS suicidal ideation intensity question(3 items), and the Rosenberg Self-efficacy (RSE) were examined to confirm the validity of SIDAS. As a result, correlations between the SIDAS and suicidal ideation intesity items of C-SSRS were significant in all items, while correlations between the SIDAS and RSE items were negative or insignificant. Third, as a result of the confirmatory factor analysis of SIDAS on all respondents and respondents with suicidal ideation, a single factor structure was appropriate for both groups. Internal consistency of SIDAS was also good. Lastly, as a result of identifying the variables affecting the suicidal plan over the past year, controllability and age were identified as significant predictive variables. SIDAS which was designed to be administered through web, can be appropriately used in Korea. It was confirmed that SIDAS is a reliable and valid suicidal ideation scale which is applicable to adults in Korea.

• Animal Bioscience
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• v.36 no.3
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• pp.404-416
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• 2023

Objective: Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods: Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results: A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 down-regulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion: Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.

• Korean Journal of Clinical Laboratory Science
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• v.55 no.1
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• pp.16-28
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• 2023

Lung adenocarcinoma accounts for about 40% of all lung cancers. With the recent development of gene profiling technology, studies on mutations in oncogenes and tumor suppressor genes, which are important for the development and growth of tumors, have been actively conducted. Companion diagnosis using next-generation sequencing helps improve survival with targeted therapy. In this study, formalin-fixed paraffin-embedded tissues of non-small cell lung cancer patients were subjected to hematoxylin and eosin staining for detecting genetic mutations that induce lung adenocarcinoma in Koreans. Immunohistochemical staining was also performed to accurately classify lung adenocarcinoma tissues. Based on the results, next-generation sequencing was applied to analyze the types and patterns of genetic mutations, and the association with smoking was established as the most representative cause of lung cancer. Results of next-generation sequencing analysis confirmed the single nucleotide variations, copy number variations, and gene rearrangements. In order to validate the reliability of next-generation sequencing, we additionally performed the existing genetic testing methods (polymerase chain reaction-epidermal growth factor receptor, immunohistochemistry-anaplastic lymphoma kinase (D5F3), and fluorescence in situ hybridiation-receptor tyrosine kinase 1 tests) to confirm the concordance rates with the next-generation sequencing test results. This study demonstrates that next-generation sequencing of lung adenocarcinoma patients simultaneously identifies mutation.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.292-297
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• 2022

Direct injection of CRISPR/Cas9 into zygotes enables the production of genetically modified nonhuman primates (NHPs) essential for modeling specific human diseases, such as Usher syndrome, and for developing novel therapeutic strategies. Usher syndrome is a rare genetic disease that causes loss of hearing, retinal degeneration, and problems with balance, and is attributed to a mutation in MYO7A, a gene that encodes an uncommon myosin motor protein expressed in the inner ear and retinal photoreceptors. To produce an Usher syndrome type 1B (USH1B) rhesus macaque model, we disrupted the MYO7A gene in developing zygotes. Identification of appropriately edited MYO7A embryos for knockout embryo transfer requires sequence analysis of material recovered from a trophectoderm (TE) cell biopsy. However, the TE biopsy procedure is labor intensive and could adversely impact embryo development. Recent studies have reported using cell-free DNA (cfDNA) from embryo culture media to detect aneuploid embryos in human in vitro fertilization (IVF) clinics. The cfDNA is released from the embryo during cell division or cell death, suggesting that cfDNA may be a viable resource for sequence analysis. Moreover, cfDNA collection is not invasive to the embryo and does not require special tools or expertise. We hypothesized that selection of appropriate edited embryos could be performed by analyzing cfDNA for MYO7A editing in embryo culture medium, and that this method would be advantageous for the subsequent generation of genetically modified NHPs. The purpose of this experiment is to determine whether cfDNA can be used to identify the target gene mutation of CRISPR/Cas9 injected embryos. In this study, we were able to obtain and utilize cfDNA to confirm the mutagenesis of MYO7A, but the method will require further optimization to obtain better accuracy before it can replace the TE biopsy approach.

• Korean Journal of Culture and Social Issue
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• v.28 no.4
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• pp.627-659
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• 2022

This study aimed to translate and validate the Positive and Negative Ex-Relationship Thoughts (PANERT), a scale measuring the positive and negative valence of thoughts about past relationships in early adulthood. For this purpose, PANERT was translated into Korean and the study surveyed on 337 single male and female adults in their 20. Then, the gender difference between major variables was analyzed. After going through item analysis, all twelve original items were used to construct the Korean version of PANERT. The confirmatory factor analysis(CFA) supported the two factors structure of the Korean version of PANERT: positive vs, negative thought content valence. Also, the reliability coefficients of each two factors were all satisfactory. As a result of a correlation analysis, the criterion-related validity of the two sub-factors was good with other related scales(Intrusive rumination scale of K-ERRI, K-DASS-21-D, and K-PANAS-Revised) except for changes of self-perception. Finally, the research model was built to examine the mediating effect of two affect responses(positive and negative) in the relationship between two thought content valences and depression. In this process, the convergence and discriminant validity of the Korean version of PANERT were confirmed and the indirect effect was also confirmed in the structural equation model. In conclusion, the Korean version of PANERT consists of two factors and twelve items in total. Also, it is a reliable and valid tool for measuring the thought content valences in the romantic relationship breakup experience of early adults.