• Journal of Microbiology and Biotechnology
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• 제13권5호
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• pp.666-672
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• 2003

Vacuolar degeneration of the gastric epithelial cells is a characteristic feature of the derangement of mucosa where Helicobacter pylori colonizes, and H. pylori vacuolating cytotoxin (VacA) has been suggested to playa key role in it. To elucidate the VacA-involved degenerative mechanism, VacA was purified, and its impact on degeneration of HeLa cells was determined. In the presence of ammonium chloride, cell vacuolation by VacA was dose-and time-dependent, however, no detectable degeneration of the cells was observed with the VacA concentration tested. A further increase of vacuolation was shown in cells pre-treated with diethyl pyrocarbonate (DEPC) , and this resulted in a change of the cell morphology to become spherical. Similar phenomena were also observed when HeLa cells were co-cultivated with intact H. pylori cells. It was remarkable to note that the degree of growth inhibition was proportional to the increase in vacuole formation, suggesting that the vacuolation rate would be critical for cell degeneration. Surprisingly, although VacA was itself inhibited by DEPC, its uptake was markedly increased by this agent, similar to that found in cells with Nabutyrate. These data indicate that the cell's tolerance of VacA transport may be critical for vacuolar degeneration and may be changeable during H. pylori inhabitation.

• 한국ITS학회 논문지
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• 제8권2호
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• pp.45-52
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• 2009

본 논문에서는 기존의 전구방식 신호등과 비교하여 LED신호등의 교통신호기 간 신호 안전성 측면의 연계 호환성을 실험 검토하고 보완 방안을 설계하여 제안하였다. 실험 분석 결과 신호의 안전성을 향상하기 위하여, 교통신호기의 출력 누설전류에 따른 LED신호등의 OFF 상태 누설전압의 과다로 인한 문제와, 누전상황 대응에 대한 문제에 대하여 보완이 필요한 것으로 나타났다. 보완을 위한 교통신호기 규격의 개선 방안은 220V 전원을 기준으로 출력 누설전류 허용 값을 기존의 8mA사양에서 3mA로 변경하고, 출력전압 평가의 기준을 기존의 25-45V에서 65-75V로 변경하는 것이며, LED신호등 규격의 개선 방안은 기존과 동일하게 95V이하에서 OFF상태를 유지하도록 함에 더하여 OFF상태에서 10Kohm이내의 임피던스를 유지하도록 하는 내용을 추가하는 것이다.

• Bulletin of the Korean Chemical Society
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• 제28권8호
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• pp.1371-1374
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• 2007

The velocity auto-correlation (VAC) function of liquid argon in the Green-Kubo formula decays quickly within 5 ps to give a well-defined diffusion coefficient because the velocity is the property of each individual particle, whereas the stress (SAC) and heat-flux auto-correlation (HFAC) functions for shear viscosity and thermal conductivity have non-decaying, long-time tails because the stress and heat-flux appear as system properties. This problem can be overcome through N (number of particles)-fold improvement in the statistical accuracy, by considering the stress and the heat-flux of the system as properties of each particle and by deriving new Green-Kubo formulas for shear viscosity and thermal conductivity. The results obtained for the transport coefficients of liquid argon obtained are discussed.