• 제목/요약/키워드: VP8

검색결과 273건 처리시간 0.026초

전통발효 식초에서 분리한 Agdohader sp.의 특성 (Some Properties of Acetobacter sp. Isolated from Traditional Fermented Vinegar)

  • 박종필;김성준
    • KSBB Journal
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    • 제8권4호
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    • pp.397-404
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    • 1993
  • Two strains were isolated from the vinegar of Korean traditional fermented rice wine and the vine gar of fermented persimmon, respectively. These strains, designated as KM and BPV, were identified as the genus Acetobacter with respect to morphological, physiological, and biochemical characteristics. The Isolates oxidized ethanol to acetate and over-oxidized acetate or lactate to CO2 and H2O. They were positive in catalase test, while being negative in oxidase, gelatin liquefaction, VP test, H2O production and indole formation tests. No ${\gamma}$-pyrones ware produced from glucose and fructose. KM was tolerant of 11% ethanol while BPV was relatively sensitive to ethanol at a higher concentration than 5%. The guanine-plus-cytosine contents of the DNA of KM and BPV strains were 53.8 and 56.6 mol%, respectively. The cellular fatty acid compositions contained in these isolates were saturated straightchain C14:0 and C16:0,, and unsaturated straight-chain C18:1. Major ubiquinone system of KM was Q-9, but that of BPV was Q-10. In morphophysiological and biochemical aspects, KM strain was similar to Acetobacter pasteurianus. However, BPV strain was different from other Acetobacter type strains.

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제주연안해역의 Vibrio parahaemolyticus 분포에 관한 연구 (Study on the Distribution of Vibrio parahaemolyticus along Cheju Coast)

  • 송민경;김만철;허문수
    • 환경생물
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    • 제25권1호
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    • pp.34-41
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    • 2007
  • 제주도내 주요 해안지역인 화북, 삼양, 대포, 중문, 표선, 안덕, 애월, 곽지에서 생산되는 해수, 갯벌, 해산물 등에서 2002년 1월부터 12월까지 V. parahaemolyticus 분포상황을 조사하였다 가검물은 총 2,880건으로 각각 해수 960건, 갯벌 960건, 해산물 960건이었으며, 분리된 V. parahaemolyticus에 대하여 생화학적, 혈청학적 실험을 실시하였다. 총가검물 2,880건에 대한 분리 동정에서 417건(14.5%)이 분리되었다. 0.85% NaCl용액을 희석 액으로 사용하여 API 20E kit를 이용한 V. parahaemolyticus 100균주의 중요한 생화학적 특성은 전 균주가 lysine, ornithine, indole, glucose, mannitol은 양성반응을 보였고, ONPG, arginine, Sodium citrate, $H_2S$, urea, tryptophane, VP, inositol, sorbitol, rhamnose, sucrose, melibiose 반응에서 음성반응을 보였고, gelatin, amygdalin, arabinose 반응에서 음성 혹은 양성반응을 보였다. 검체별 분리율은 해수가 960건 중 161건(16.8%), 갯벌이 960건 중 137건(14.3%), 해산물이 960건 중 119건(12.4%)이었다. 지역별 분리율은 화북 14.4%(52/360), 삼양 15.3%(55/360), 대포 13.6%(49/360), 중문 18.3%(66/360), 표선 13.1%(47/360), 안덕 16.4% (59/360), 애월 12.5% (45/360), 곽지 12.2% (44/360)이었다. 분리된 V. parahaemolyticus 417균주를 대상으로 지역별, 검체별 분포를 조사한 결과 중문 지역이 18.3%(66/360)로 가장 높았고, 검체별로는 해수가 16.8% (161/960)로 가장 높았다.

Molecular and Epidemiological Characterization of Enteroviruses Isolated in Chungnam, Korea from 2005 to 2006

  • Baek, Kyung-Ah;Park, Kwi-Sung;Jung, Eun-Hye;Chung, Eun-Hee;Park, Joon-Soo;Choi, Hwa-Jung;Baek, Seung-Hwa;Jee, Young-Mee;Cheon, Doo-Sung;Ahn, Gwang-Sook
    • Journal of Microbiology and Biotechnology
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    • 제19권9호
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    • pp.1055-1064
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    • 2009
  • Enteroviruses were identified and characterized from patients with aseptic meningitis and other enterovirus-related diseases in Chungnam, Korea from 2005 to 2006. Enteroviruses were isolated from 79 of 519 cases (15.2%) in 2005, and 37 of 386 cases (9.6%) in 2006. Based on partial VP1 sequencing, a total of 116 enterovirus isolates were resolved into 13 types. Prevalent among the Chungnam isolates were echovirus 18 and coxsackievirus B5 in 2005, and echoviruses 5 and 25 in 2006. This is the first time echoviruses 5 and 18 have been identified in Korea since enterovirus surveillance began there in 1993. The temporal distribution of enterovirus epidemics in Chungnam showed a remarkable seasonal pattern, with cases occurring during most of the three months of the summer from June to August. The highest rate of enterovirus-positive cases occurred in patients less than 1 year of age. The ratio of male to female enterovirus-positive patients was approximately 1.8:1. Comparison of the VP1 amino acid sequences of the 15 coxsackievirus B5 isolates with reference strains revealed that all Chungnam isolates are substituted at positions 23 (V231), 19 (S19G), 75 (Y75F), and 95 (N95S). Upon comparing the nine ECV5 isolates with foreign strains, it was found that only the Chungnam isolates, with the exception of Kor06-ECV5-239cn, have P at position 153 and F at position 146. The three ECV9 isolates from 2006 show alterations at amino acids 36, 148, and 154 outside of the BC-loop and at position 84 in the BC-loop, whereas the seven isolates from 2005 and the other ECV9 strains in the database only show the alteration at position 84 (D, I, N, S). The five ECV25 isolates have an S residue at position 134, whereas most of the foreign strains have an N residue.

한반도 중부권 지각물질의 구조와 물성연구(2) : 퇴적암류 코아시료의 탄성파 속도와 점재하 강도 비교 (Structure and physical properties of Earth Crust material in the Middle of Korean Peninsula(2) : Comparison between elastic Velocity and point-load of core specimen of sedimentary rocks.)

  • 송무영;황인선
    • 지질공학
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    • 제3권1호
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    • pp.21-37
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    • 1993
  • 석회암, 사암과 셰일의 퇴적암 코아시료를 대상으로 암석의 비중, 공극률, 함수율 등을 구하고 탄성파 전파 속도와 점재하 강도 지수를 측정하였다. 이들 암석물성 사이의 관계로부터 석회암에 대한 밀도와 탄성파 속도의 관계는 $Vp=4085d^2-20747d+303,{\;}V_s=3899d^2-21442d+318$의 다소 곡선 경향을 나타낸다. 또한 밀도가 높은 셰일의 탄성파 속도가 사암에서보다 작으며 이는 셰일의 층리 영향띠문인 것으로 보인다. 석회암, 사암, 셰일의 P파 속도와 S파 소ㄷ도는 대체로 직선 양상을 보여주며 각 관계식은 석회암에서는 $V_s=0.26V_p+1041.6m/sec,{\;}사암은{\;}V_s=0.43V_p+424.2m/sec$, 셰일에서는 r= 0.86으로 나타났다. 석회암에 대한 점재하 강도 시험에 의하면 점재하 강도 이방성을 시료가 다소 호상구조를 보이더라도 뚜렷하지 않았다. $30^{\circ}-60^{\circ}$내외의 변화를 보이는 층리면 경사각은 직경방향과 축방향의 점재하 지수에 큰 영향을 미치지 않은 것으로 생각된다. 점재하 강도 실험결과 점재하강도 지수가 증가하면 P파 속도도 증가하지만 상관관계가 뚜렷하지 않았다.

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소아 흉부 CT 검사에서 관전압 80 kVp 조건으로 스캔 모드별 방사선량 감소와 화질 평가 (The Evaluation of Dose Reduction and Quality of Images According to 80 kVp of Scan Mode Change in Pediatric Chest CT)

  • 김구;김경립;이은숙;조희정;성순기;문슬지아;곽종혁
    • 한국콘텐츠학회논문지
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    • 제19권8호
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    • pp.284-292
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    • 2019
  • 소아 흉부 CT 검사 시 방사선 피폭을 최소화 하면서 진단적으로 가치가 높은 영상을 얻기 위해서 Helical mode, High-pitch mode, Volume Axial mode를 적용하여서 선량, 검사시간, 화질을 비교하여 유용성을 평가하고자 한다. Revolution(GE Healthcare, Wisconsin USA)을 이용하여 PBU-70팬텀을 Helical mode, High-pitch mode, Volume Axial mode로 각각의 그룹으로 나누어서 관전압 80kVp, 조건으로 30회 검사를 실시하였다. 영상을 획득 한 후에 각각의 영상에 심장(Heart), 뼈(Bone), 폐(Lung), Back-ground air에 ROI를 설정하고 CT number(HU)와 noise(SD)값을 측정하여 평균값을 구하고 SNR과 CNR을 측정하였고, 장비에서 직접 제공하는 DLP값 비교하였다. 통계적인 유의성을 확인해보기 통계 분석은 SPSS 21.0을 사용하여 ONE-WAY-ANAOVA를 시행하였다. 본 실험을 통해 검사 시 volume axial mode 사용 시 가장 적은 선량으로 영상의 화질 저하 없이 빠른 시간에 검사가 가능하였다. 16cm의 detector coverage 가 모든 소아 흉부 CT검사에 적용하기에 제한점이 있으나 가능한 소아환자에 있어 적극적인 활용을 추천하며 volume axial mode의 다른 검사부위 적용에 대한 지속적인 연구가 필요하리라 사료된다.

ORIGINAL ARTICLE - 이동형 구내방사선촬영기로 촬영한 치근단 방사선촬영과 파노라마방사선촬영의 흡수선량과 유효선량 평가 (A absorbed and effective dose from the full-mouth periapical radiography using portable dental x-ray machine and panoramic radiography)

  • 한원정
    • 대한치과의사협회지
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    • 제50권7호
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    • pp.420-430
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    • 2012
  • Purpose: The purpose of this study was to measure the absorbed dose and to calculate the effective dose for full-mouth periapical radiography using the portable dental x-ray machine and panoramic radiography Material and Method: Thermoluminescent chips were placed at 25sites throughout the layers of the head and neck of a tissue-equivalent human skull phantom. The man phantom was exposed with the portable dental x-ray machine and panoramic unit. During full-mouth periapical radiography the exposure setting was 60 kVp, 2 mA and 0.15 ~ 0.25 seconds, while during panoramic radiography the selected exposure setting was 72 kVp, 8 mA and 18 seconds. Absorbed dose measurements were obtained and equivalent doses to individual organs were summed using ICRP 103 to calculate of effective dose. Result: In the full-mouth periapical radiography, the highest absorbed dose was recorded at the mandible body follow with submandibular glands and cheek. Using panoramic unit, the highest absorbed dose was parotid glands and the following was back of neck and submandibular glands. The effective dose in full-mouth periapical radiography using portable dental x-ray machine was 46 ${\mu}Sv$. In panoramic radiography, the effective dose was 38 ${\mu}pSv$. Conclusion: It was recommended to panoramic radiography for general check in the head and neck area because that the effect dose in the panoramic radiography was lower than the dose in the full-mouth periapical radiography using portable dental x-ray machine.

Effect of Production Phase on Growth, Enzyme Activities and Feed Selection of Broilers Raised on Vegetable Protein Diet

  • Hossain, M.A.;Islam, A.F.;Iji, P.A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권11호
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    • pp.1593-1599
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    • 2014
  • This study consisted of two experiments, conducted to assess the impact of phase at which vegetable protein (VP) diets are introduced to broiler chicks, and preference of birds for diets based on soybean or canola meal (CM). Two hundred and ten day-old Cobb 500 chicks were randomly distributed into five dietary groups in the main experiment. One group was fed on animal protein (AP) diet all through to 21 days of age; two other groups were started on AP diet for 7 days and then switched to diets containing soybean meal (AP-SBM) or AP-CM, while two other diets (SBM-AP and CM-AP) were started on one of the VP diets for 7 days and then switched to AP diet. A sub-experiment on thirty birds raised on a commercial diet to 7 days was used in a feed selection test to quantify the preference of birds for the diets containing mainly CM or SBM. Chicks were reared under similar care and management conditions and the diets were iso-caloric and iso-nitrogenous. Results of the main experiment showed that chicks on CM-AP diet ate more (p<0.05) than those on the other diets up to day 7. Body weight gain was highest (p<0.001) on the AP-SBM diet while birds on the CM-AP diet weighed the least at 7 d. Feed intake, body weight gain, feed conversion ratio, mortality, bone growth, visceral organ development, and activities of digestive enzymes were similar between the groups from hatch to 21 days of age. Results of the second sub-experiment showed that chicks preferred the CM-based diets to the SBM-based diets at 8 to 14 d (p<0.001) and 15 to 21 d (p<0.01) when given a choice. Overall, the birds were not affected by the nature of the starter diet although they tended to prefer the canola to soybean diets.

Comparative Study of Outcomes between Shunting after Cranioplasty and in Cranioplasty after Shunting in Large Concave Flaccid Cranial Defect with Hydrocephalus

  • Oh, Chang-Hyun;Park, Chong-Oon;Hyun, Dong-Keun;Park, Hyung-Chun;Yoon, Seung-Hwan
    • Journal of Korean Neurosurgical Society
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    • 제44권4호
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    • pp.211-216
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    • 2008
  • Objective : The cranioplasty and ventriculoperitoneal (VP) shunt operation have been used to treat a large cranial defect with posttraumatic hydrocephalus (PTH). The aim of this study was to evlauate the difference of outcomes between in the shunting after the cranioplasty (group 1) and the cranioplasty after the shunting (group 2) in a large flaccid cranial defect with PTH. Methods : In this study, a retrospective review was done on 23 patients undergoing the cranioplasty and VP shunt operation after the decompressive craniectomy for a refractory intracranial hypertension from 2002 to 2005. All of 23 cases had a large flaccid concave cranial defect and PTH. Ten cases belong to group 1 and 13 cases to group 2. The outcomes after operations were compared in two groups 6 months later. Results : The improvement of Glasgow outcome scale (GOS) was seen in 8 cases (80.0%) of total 10 cases in group 1, and 6 cases (46.2%) of 13 cases in group 2. Three (75.0%) of 4 cases with hemiparesis in group 1 and 3 of 6 cases (50.0%) in group 2 were improved. All cases (2 cases) with decrease of visual acuity were improved in each group. Dysphasia was improved in 3 of 5 cases (60%) in group 1 and 4 of 6 cases (66.6%) in group 2. Conclusion : These results suggest that outcomes in group 1 may be better than in group 2 for a large flaccid concave cranial defect with PTH.

치조병소에 대한 X-선학적 비교연구 (A COMPARATIVE STUDY OF ROENTGENOGRAPHIC APPEARANCE OF PERIODONTAL OSSEOUS LESIONS)

  • 김재덕
    • 치과방사선
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    • 제10권1호
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    • pp.63-68
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    • 1980
  • The purpose of this article is to re-examine the roentgenographic appearance of alveolar osseous in an effort to determine the value of the orthopantomogram in the diagnosis of periodontal disease. A total of 158 osseous lesions from 13 human dry skulls were studied. 14 Ultra-speed periapical films, 2 bitewing films and Kodak panoramic film are used to obtain radiographs of all defects. The bisecting technic was used, with a target-film distance of. 8 inches and exposure factors of 70 kVp, 10MA and 0.4 sec. at anterior teeth, 0.6 see at posterior teeth in exposure time. For orthopantomogram, Panoura Eight-C was used with a exposure factors of 90kVp, 10MA. and 15sec. exposure time. All films were developed in a light. tight darkroom at 68°F for 4½ minutes. Comparison of orthopantomogram and intraoral films on the view-box was carefully studied in relation to the types of osseous defect visually evident; Proximal intraosseous defects, Interproximal craters, Interproximal hemisepta, Furcal defects on multirooted teeth, and Facial or Lingual one-walled defects. The results obtained were as follows; 1. Proximal osseous defects throughout the dental arches and furcal defects on facial and lingual surfaces of multirooted teeth can be identified with a high degree of accuracy from their orthopantomographic appearances. 2. Lesions on facial or lingual surface of the alveolar arches are rather difficult to locate or recognize on the dental radiographs. 3. In determining whether the proximal and furcal lesions are located facially or lingually, Orthopantomogram is superior to the conventional film Orthopantomogram obtained with standardization of head in proper position revealed the complete visualization of alveolar bone without showing occlusal surface of molars and proximal superimposition of teeth. Thus, on the standardized orthopantomograms, The roentgenographic characteristics of each defect were determined.

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방사선 투과 및 불투과성 물질에 따른 감약 차이의 분석 : DECT 검사 중심으로 (Analysis of Attenuation Differences According to Radiolucent and Radiopague Materials : Based on DECT (Dual Energy Computed Tomography))

  • 장현철;김윤신;김현주
    • 한국산학기술학회논문지
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    • 제15권3호
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    • pp.1584-1589
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    • 2014
  • 본 연구는 경기도 소재 S 대학병원에서 2013년 7월부터 8월까지 GE 사의 DECT(Dual Energy Computed Tomography)를 이용하여 방사선 투과 및 불투과성 물질을 임의로 선정, 에너지 영역 대별로 투과 및 불투과성 물질의 CT 값을 분석하였다. 또한, CT 값 분석 법을 이용하여 기존의 SECT(Single Energy Computed Tomography)에서 적용되는 120kVp CT 값과 가장 유사한 에너지 영역대를 도출하였으며 임상 적용 시 가시 영역 범위 내에서 대조도를 주었을 때 가장 유용하며 적절한 물질을 알아보았다. 결론으로 생리식염수, 메틸셀룰로스, 초음파용 젤과 같이 밀도가 낮고 수분 함유량이 많은 경우 90KeV 이후 CT 값의 감소가 거의 없었으며, 공기와 조영제처럼 밀도가 매우 낮거나 높은 물질의 경우 에너지의 영향을 적게 받는 것으로 분석되었으며 메틸셀룰로오스와 초음파 젤의 경우 임상 적용 시 가장 유용성이 있는 물질로 사료된다.