• 식물분류학회지
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• 제37권2호
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• pp.87-108
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• 2007

콩과의 화분학적 자료를 제공하고 분류학적 연구에 기여하고자, 한국에서 생육하는 콩과 목본식물 12속 31분류군의 화분형태를 광학현미경과 주사형전자현미경으로 관찰하였다. 본 콩과의 화분립 형태는 단립이었으며, 적도면 입상은 약장구형 혹은 드물게 아장구형으로 나타났다. 화분립의 발아구는 적도면에서 일렬로 배열하는 삼공구형이거나 혹은 드물게, 한국에서 생육하는 콩목의 다른 과에서는 나타나지 않는, 삼복개형이었다. 본 과는 표면무늬에서 평활상, 미세공상, DMIP상, 세공상, 다각형 세공상, MAP상, MPG상, PMAP상, VPPS상, VP상, 과립상, 난선상, 망상, 세망상과 같은 다양성을 보여주었다. 화분학적 특징은 개느삼속이 고삼속으로 합병되어야 함을 지지하였고, 골담초속(Galegeae족)과 Sophoreae족 내 Sophora 그룹(Maackia, Sophora, Echinosophora)이 측정된 모든 특징과 표면무늬에서 서로 상당히 유사하였다. 발아구형, 화분립형태 그리고 표면무늬의 차이점에 근거하여 콩과의 11속이 인지되었고, 한국에서 생육하는 목본 콩과의 속수준의 화분검색표와 싸리속의 화분형검색표가 작성되었다. 조사된 Coronilla속과 등나무속 내 분류군들이 표면무늬에 의해 구별이 가능했다.

• 대한지역사회영양학회지
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• 제25권3호
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• pp.236-245
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• 2020

Objectives: Cardiovascular disease is a major cause of death in the elderly in Korea. Increased arterial stiffness is linked to risk of cardiovascular disease and mortality. The purpose of this study was to investigate the relationship between arterial stiffness and physical activity in the elderly. Methods: A total of 209 older adults (110 men and 99 women) participated in this study. Arterial stiffness of subjects such as brachial-ankle pulse wave velocity (baPWV) and ankle brachial pressure index (ABI) was measured using a non-invasive vascular screening device (VP-1000 Plus, Omron, Kyoto, Japan). The interviewed Korean version of the international physical activity questionnaire short form (IPAQ-SF) was used to evaluate subject's physical activity level and classify subjects as active or inactive group based on the time spent doing moderate to vigorous physical activity (MVPA). Results: The mean age of total subjects was 75.3 ± 5.6 years. There was no significant difference in sex distribution between the active group (39.7%) and inactive group (60.3%). The baPWV (1,758.1 ± 375.2 cm/sec) of the active group was significantly lower than that (1,969.7 ± 372.3 cm/sec) of the inactive group (P < 0.05). There was a significant inverse association between time spent in MVPA and baPWV (r = -0.245, P < 0.01). Conclusions: This study suggests that physical activity programs for older adults are needed to prevent arteriosclerosis.

• 한국임상수의학회지
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• 제23권3호
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• pp.300-307
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• 2006

In the present study, methods of the reverse transcription-polymerase chain reaction(RT-PCR) were evaluated for the rapid detection and differentiation of transmissible gastroenteritis virus(TGEV), porcine epidemic diarrhea virus(PEDV) and rotavirus in piglets suffering from diarrhea. For the purposes, the PCR conditions were first confirmed for the amplification of VP7 gene of rotavirus and N gene of TGEV and PEDV using each specific primers and their annealing temperature. Multiplex RT-PCR methods were further determined to distinguish these viral infections and the results are as follows. For the specific amplification of these viral genes, the reliable PCR condition was determined as 30 cycles of reaction consisting each 1 min of denature at $94^{\circ}C$, annealing at $42^{\circ}C$ and polymerization at $72^{\circ}C$ with 1.0 mM $MgCl_2$. It was able to differentiate these viral infections in the intestines and feces of piglets suffering from diarrhea by duplex PCR for TGEV and PEDV and single PCR for rotavirus with a primer-annealing temperature of $42^{\circ}C$. When the multiplex RT-PCR were undertaken for the field samples, 17 cases of PEDV and 5 cases of rotavirus infections were differential diagnosed in a total of 92 samples of intestines and feces of the piglets with diarrhea.

• 한국산학기술학회논문지
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• 제11권9호
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• pp.3517-3523
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• 2010

본 논문은 이중 에너지 DR(Digital Radiography) 방식 중, 단일 조사 X-선(single shot X-ray exposure) 장치와 이중 모드 검출기 모듈 (Low Energy Detector & High Energy Detector)을 이용한 이중 X-선 이미징이 가능한 검출기 모듈에 관한 연구이다. 상용 BIS(baggage inspection system)에서 사용되고 있는 X-선 발생장치의 스펙트럼과 이중 모드 검출기에 대한 특징 및 방사선적 특성을 분석하여 새롭게 제안 할 검출기 모듈의 최적 설계 방향을 기술하고 상용화된 용화된 LED 및 HED 검출기와 새롭게 제안 한 검출기 모듈에 대해 전기적, 광학적, 방사선적 특성 실험을 실시하여, 새롭게 제안된 검출기 모듈이 BIS 용도로 사용 가능함을 증명하였다. 새롭게 제안 된 검출기 모듈이 적용된 BIS에 대해, 기본 특성 실험에 대한 X-선 영상을 획득하여 실험 및 분석을 실시하였다.

• 대한디지털의료영상학회논문지
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• 제11권1호
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• pp.5-13
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• 2009

There are several reasons to take X-ray in case of inpatients. Some of them who cannot ambulate or have any risk if move are taken portable X-ray at their wards. Usually, in this case, many other people-patients unneeded X-ray test, family, hospital workers etc-are indirectly exposed to X-ray by scatter ray. For that reason I try to be aware of free space scatter dose accurately and make the point at issue of portable X-ray better in this study. kVp dose meter is used for efficiency management of portable X-ray equipment. Mobile X-ray equipment, ionization chamber, electrometer, solid water phantom are used for measuring of free space scatter dose. First of all the same surroundings condition is made as taken real portable X-ray, inquired amount of X-ray both chest AP and abdomen AP most frequently examined and measured scatter ray distribution of two tests individually changing distance. In the result of measuring horizontal distribution with condition of chest AP it is found that the mAs is decreased as law of distance reverse square but no showed mAs change according to direction. Vertical distribution showed the mAs slightly higher than horizontal distribution but it isnt found out statistical characteristic. In abdomen AP, compare with chest AP, free space scatter dose is as higher as five-hundred times and horizontal, vertical distribution are quite similar to chest AP in result. In portable X-ray test, in order to reduce the secondary exposure by free space scatter dose first, cut down unnecessary portable order the second, set up the specific area at individual ward for the test the third, when moving to a ward for the X-ray test prepare a portable shielding screen. The last, expose about 2m apart from patients if unable to do above three ways.

• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.511-515
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• 2007

White-spot syndrome virus (WSSV) is a devastating, infectious virus affecting shrimp. Although sensitive techniques involving PCR have been developed to assist farmers in screening shrimp (brood stock) for WSSV prior to stocking ponds, such practices have not yet been applied in Korea. Despite the rationality of implementing screening, there has been some doubt as to whether the stocking of WSSV-PCR-negative fly epidemiologically decreases white-spot disease outbreaks. Here, we report a retrospective analysis of data from shrimp farms in the western coast of Korea where WSSV-PCR-negative brood stocks were used to stock rearing ponds. A total of 366 shrimp from Heuksan Island were sampled for WSSV with PCR. Of the tested shrimp, 7.2% (28 brood stocks) were identified as WSSV positive; only WSSV-PCR-negative shrimp were used for brood stocks. Total unit production (final shrimp production/ the area of the ponds) was higher, at 1.96, in ponds where WSSV-PCR-negative shrimp were used, as compared with 1.02 in other ponds in Korea in 2004. This retrospective analysis of WSSV in Korea may be useful to the shrimp aquaculture industry, suggesting a testable hypothesis that may contribute to the eventual control of WSSV outbreaks.

• Journal of Microbiology and Biotechnology
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• 제30권1호
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• pp.38-43
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• 2020

Hand, foot, and mouth disease (HFMD) is caused by enterovirus 71 (EV71) in infants and children under six years of age. HFMD is characterized by fever, mouth ulcers, and vesicular rashes on the palms and feet. EV71 also causes severe neurological manifestations, such as brainstem encephalitis and aseptic meningitis. Recently, frequent outbreaks of EV71 have occurred in the Asia-Pacific region, but currently, no effective antiviral drugs have been developed to treat the disease. In this study, we investigated the antiviral effect of salvianolic acid B (SalB) on EV71. SalB is a major component of the Salvia miltiorrhiza root and has been shown to be an effective treatment for subarachnoid hemorrhages and myocardial infarctions. HeLa cells were cultured in 12-well plates and treated with SalB (100 or 10 ㎍/ml) and 10⁶ PFU/ml of EV71. SalB treatment (100 ㎍/ml) significantly decreased the cleavage of the eukaryotic eIF4G1 protein and reduced the expression of the EV71 capsid protein VP1. In addition, SalB treatment showed a dramatic decrease in viral infection, measured by immunofluorescence staining. The Akt signaling pathway, a key component of cell survival and proliferation, was significantly increased in EV71-infected HeLa cells treated with 100 ㎍/ml SalB. RT-PCR results showed that the mRNA for anti-apoptotic protein Bcl-2 and the cell cycle regulator Cyclin-D1 were significantly increased by SalB treatment. These results indicate that SalB activates Akt/PKB signaling and inhibits apoptosis in infected HeLa cells. Taken together, these results suggest that SalB could be used to develop a new therapeutic drug for EV71-induced HFMD.

• 한국동물위생학회지
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• 제32권4호
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• pp.315-323
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• 2009

Foot-and-mouth disease (FMD) virus exists in seven serotypes and is known to be a highly contagious disease that is hard to eradicate from the world. The O, A, Asia1 and SAT2 serotypes commonly infected cattle, sheep and goats during 2007~2009 throughout the world. In particular, the outbreak of the Asia1 serotype in China appeared in all areas from 2005 and is still present. Surprisingly, in 2009, Taiwan reported the first outbreak of the type O serotype since 2001. Then type A appeared in China for the first time since the early 1960s. The virus shows a close relationship to the viruses from Southeast Asia suggesting one or more recent introductions into China in the OIE reports. Recently the subtype of A/Iran05 spread to nearby countries exhibiting genomic evolution. The use of molecular epidemiology is an important tool in understanding and consequently controlling the FMD virus. The phylogenetic analysis with VP1 gene was especially useful for molecular epidemiological studies and showed the same pattern which matches with serotype classification. This paper describes basic information about the disease, and the serotype-specific characteristics and evolution to perform molecular epidemiological analysis. Furthermore, we show the importance of the genetic evolution on the FMD serotypes in global surveillance and molecular epidemiology of FMD for outbreak investigation.

• JSTS:Journal of Semiconductor Technology and Science
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• 제2권4호
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• pp.259-267
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• 2002

We present a novel 3D fabrication method with single X-ray process utilizing an X-ray mask in which a micro-actuator is integrated. An X-ray absorber is electroplated on the shuttle mass driven by the integrated micro-actuator during deep X-ray exposures. 3D microstructures are revealed by development kinetics and modulated in-depth dose distribution in resist, usually PMMA. Fabrication of X-ray masks with integrated electrothermal xy-stage and electrostatic actuator is presented along with discussions on PMMA development characteristics. Both devices use $20-\mu\textrm{m}$-thick overhanging single crystal Si as a structural material and fabricated using deep reactive ion etching of silicon-on-insulator wafer, phosphorous diffusion, gold electroplating, and bulk micromachining process. In electrostatic devices, $10-\mu\textrm{m}-thick$ gold absorber on $1mm{\times}1mm$ Si shuttle mass is supported by $10-\mu\textrm{m}-wide$, 1-mm-long suspension beams and oscillated by comb electrodes during X-ray exposures. In electrothermal devices, gold absorber on 1.42 mm diameter shuttle mass is oscillated in x and y directions sequentially by thermal expansion caused by joule heating of the corresponding bent beam actuators. The fundamental frequency and amplitude of the electrostatic devices are around 3.6 kHz and $20\mu\textrm{m}$, respectively, for a dc bias of 100 V and an ac bias of 20 VP-P (peak-peak). Displacements in x and y directions of the electrothermal devices are both around $20{\;}\mu\textrm{m}$at 742 mW input power. S-shaped and conical shaped PMMA microstructures are demonstrated through X-ray experiments with the fabricated devices.

• 한국정보통신학회논문지
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• 제14권1호
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• pp.16-22
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• 2010

심전도 신호 분석 및 부정맥 분류는 환자를 진단하고 치료하는데 중요한 역할을 한다. 부정맥은 맥박이 불규칙한 상태로 심실빈맥(VT)이나 심실세동(VF) 환자에게 심각한 위협이 될 수 있다. 심방조기수축(APC)과 상심실성빈맥(SVT), 심실조기수축(PVC)은 심실빈맥(VT)만큼 치명적이지는 않지만 심장질환을 진단하는데 중요한 부정맥이다. 본 논문은 2~3개의 부정맥 분류만을 고려한 기존의 방법을 극복하고 다양한 부정맥을 분류하기 위한 새로운 방법을 제시한다. 심전도 신호의 특징 추출을 위해서 EMD 방법으로 신호를 분해하여 IMFs를 얻는다. 입력 데이터의 양은 분류기 성능에 영향을 미치므로 신호 데이터의 차원을 감소시키기 위해 Burg 알고리즘을 IMFs에 적용하여 AR 계수를 구하고 여러 개의 이진 분류기를 결합한 다중 클래스 SVM의 입력으로 사용한다. 최적의 SVM 성능 파라미터를 선택하고 부정맥 분류에 적용한 결과 검출의 정확성은 96.8%~99.5%였다. 실험 결과는 제안한 EMD 방법에 의한 전처리 및 특징 추출과 다중 클래스 SVM에 의한 부정맥 분류의 유용성을 보여준다.