• Title/Summary/Keyword: VFA

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Effects of Feeding Heat Treated Protein and Mineral Complex on In Vitro Fermentation Characteristics, Milk Production and Composition of Holstein Dairy Cows (열처리 단백질-광물질 복합제제 첨가가 In Vitro 발효성상과 착유우의 유량 및 유성분에 미치는 영향)

  • Choi, N.J.;Bae, G.S.;Nam, K.P.;Chang, M.B.;Um, J.S.;Ko, J.Y.;Ha, J.K.
    • Journal of Animal Science and Technology
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    • v.44 no.5
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    • pp.541-548
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    • 2002
  • This study, consisting of two experiments, was conducted to determine the effects of feeding heat treated protein and mineral complex (HPM) on milk production and composition, and ruminal fermentation of Holstein dairy cows. In in vitro experiment, HPM levels were 0, 0.2, 1 and 2%, and Timothy hay, which was substrate, was milled as 1 mm size, and the effects of HPM on pH, ammonia and VFA were analyzed after incubation times of 0, 6, 12, 24 and 48 h, respectively. The pH and ammonia production were not significantly different between treatments during the incubation. In addition, generally, total VFA and individual VFA were not affected by HPM on 0, 6 and 24 h. While, total VFA and individual VFA were increased in 0.2% and 1% of HPM supplemented treatments, but decreased in 2% of HPM treatment compared with control on 12 h. On 48 h, total VFA and individual VFA were increased in HPM treatments compared to control (P<0.05). However, A/P ratio was not affected by HPM supplementation. Gas production was higher in HPM treatment compared to control on 24 h (P<0.05) and 48 h (P<0.05). In lactating experiment, fourteen lactating Holstein cows were used for 4 months in a cross over experimental design. There were two treatments; no added HPM as a control and 0.2% of HPM added as a test treatment. Daily milk yield (P<0.001), 4% FCM (P<0.001), milk protein (P<0.05) and SNF (solid not fat; P<0.05) were increased in HPM treatment compared to control. While, milk fat, MUN (milk urea nitrogen) and SCC (somatic cell count) were not significantly different between treatments.

Practical Node Deployment Scheme Based on Virtual Force for Wireless Sensor Networks in Complex Environment

  • Lu, Wei;Yang, Yuwang;Zhao, Wei;Wang, Lei
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.3
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    • pp.990-1013
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    • 2015
  • Deploying sensors into a target region is a key issue to be solved in building a wireless sensor network. Various deployment algorithms have been proposed by the researchers, and most of them are evaluated under the ideal conditions. Therefore, they cannot reflect the real environment encountered during the deployment. Moreover, it is almost impossible to evaluate an algorithm through practical deployment. Because the deployment of sensor networks require a lot of nodes, and some deployment areas are dangerous for human. This paper proposes a deployment approach to solve the problems mentioned above. Our approach relies on the satellite images and the Virtual Force Algorithm (VFA). It first extracts the topography and elevation information of the deployment area from the high resolution satellite images, and then deploys nodes on them with an improved VFA. The simulation results show that the coverage rate of our method is approximately 15% higher than that of the classical VFA in complex environment.

Effects of Chemical Additives Containing Al and Ca on Volatile Fatty Acids and Nitrogen Contents of Litter (Al과 Ca을 함유한 화학제재의 첨가가 깔짚내 휘발성 지방산과 질소 함량에 미치는 영향)

  • Choi, In-Hag;Choi, Jung-Hoon
    • Journal of Environmental Science International
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    • v.17 no.2
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    • pp.225-232
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    • 2008
  • The objectives of this study were conducted to determine the effects of two chemical amendments on volatile fatty acids (VFA) and nitrogen contents in poultry litter after broiler chicks were raised in poultry houses for 6 weeks. Two different additives were applied as a top dressing to the litter at a rate of $AlCl_3{\cdot}6H_2O$ (200 g)+$CaCO_3$ (50 g) or Alum (200 g)+$CaCO_3$ (50 g)/kg of rice bran; untreated litter served as controls. Application of $AlCl_3+CaCO_3$ and Alum+$CaCO_3$ reduced total VFA contents by 67% and 51% at 6 weeks, respectively, compard to the control groups. The decrease in litter pH with two chemical treatments results in decreased proportion of VFA and increased nitrogen contents of the litter. These results indicate that treating $AlCl_3+CaCO_3$ and Alum+$CaCO_3$ to poultry litter offers the potential for reducing an environmental impact.

Comparison of Digestive Function Among Rabbits, Guinea-Pigs, Rats and Hamsters. II. Digestive Enzymes and Hindgut Fermentation

  • Yu, Bi;Chiou, Peter Wen-Shyg;Kuo, Chung-Yi
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.11
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    • pp.1508-1513
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    • 2000
  • The aim of this trial was to study the response of laboratory animals including omnivores (rats) and herbivores (rabbits, guinea pigs and hamsters) to the same level of dietary fiber on their digestive enzymes and hindgut fermentation. Ten weanling animals of each of four species, rabbits, guinea-pigs, rats and Syrian hamster, were fed a basal diet of 18% crude protein and 10% crude fiber for six weeks. The digesta and tissue of each intestinal segment were collected to measure the activity of digestive enzymes. Rabbits contained the highest secreted pepsin activity in the stomach, whereas rats contained the highest protease and ${\alpha}-amylase$ activity in the small intestine, and lower fibrous hydrolases in the hindgut than rabbits, guinea pigs and hamsters. The total VFA productions in the caecum and colon were highest in rats, followed by hamsters and rabbits, while the guinea pigs contained the lowest VFA and a different pattern of VFA molar ratio from the other laboratory animals. The degree of hindgut fermentation in these laboratory animals was in reverse to the trend for their fiber digestion.

Piggery Slurry Composting Using Batch Operating Autothermal Thermophilic Aerobic Digestion System

  • Ahn, Hee K.;Choi, Hong L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.2
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    • pp.273-279
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    • 2006
  • The performance of an autothermal thermophilic aerobic digestion (ATAD) system was studied to determine if nitrogen loss, as ammonia, was affected by an exhaust gas condenser. The system was run with and without a condenser while treating $8m^3$ of piggery slurry for 8 days. The system with a condenser (SWC) maintained the reactor temperatures above $40^{\circ}C$ for 2 days during the 8 days run, while the system without a condenser (SWOC) remained above $40^{\circ}C$ for 6 days. The SWC maintained the reactor temperatures mostly at mesophilic conditions while the SWOC at thermophilc conditions. Differences in operation conditions for the two runs were mainly caused by differences in atmospheric temperatures. Soluble chemical oxygen demand (SCOD) and volatile solids (VS) removal efficiencies of the SWC (SCOD: 62%, VS: 41%) were higher than those of the SWOC (SCOD: 40%, VS: 20%). The total Kjeldal nitrogen (TKN) removal efficiency of the SWC (7%) was less than that of the SWOC (25%). The concentration of total volatile fatty acids (VFA) in the SWC was observed to be lower than the threshold value of 0.23 g total VFA/L after 6 days, while the SWOC progressed below the threshold value after 3 days. No offensive odor emissions were observed in either run, which suggest that the use of the ATAD system may be a good odor removal strategy.

Factors Influencing Biohydrogenation and Conjugated Linoleic Acid Production by Mixed Rumen Fungi

  • Nam, In-Sik;Garnsworthy, Philip C.
    • Journal of Microbiology
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    • v.45 no.3
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    • pp.199-204
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    • 2007
  • The objective of this study was to evaluate the effect of soluble carbohydrates (glucose, cellobiose), pH (6.0, 6.5, 7.0), and rumen microbial growth factors (VFA, vitamins) on biohydrogenation of linoleic acid (LA) by mixed rumen fungi. Addition of glucose or cellobiose to culture media slowed the rate of biohydrogenation; only 35-40% of LA was converted to conjugated linoleic acid (CLA) or vaccenic acid (VA) within 24 h of incubation, whereas in the control treatment, 100% of LA was converted within 24 h. Addition of VFA or vitamins did not affect biohydrogenation activity or CLA production. Culturing rumen fungi at pH 6.0 slowed biohydrogenation compared with pH 6.5 or 7.0. CLA production was reduced by pH 6.0 compared with control (pH 6.5), but was higher with pH 7.0. Biohydrogenation of LA to VA was complete within 72 h at pH 6.0, 24 h at pH 6.5, and 48 h at pH 7.0. It is concluded that optimum conditions for biohydrogenation of LA and for CLA production by rumen fungi were provided without addition of soluble carbohydrates, VFA or vitamins to the culture medium; optimum pH was 6.5 for biohydrogenation and 7.0 for CLA production.

Effect of ammonium nitrogen in anaerobic biofilter using live-stock-wastewater (축산폐수의 혐기성 고정법에 있어서 암모니아성 질소의 영향)

  • Eom, Tae-Kyu;Lim, Jung-Won
    • Journal of Korean Society of Water and Wastewater
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    • v.11 no.4
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    • pp.43-53
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    • 1997
  • In this research, the synthetic livestock wastewater was prepared to study the characteristics of organic matter removal, the change of VFA production, and the amount of gas production with respect to the change of ammonium nitrogen concentration in the waste using anaerobic fixed bed process, which is an anaerobic biofilm process. The HRT and operation temperature were 1 day and $35{\pm}1^{\circ}C$, respectively. Also, the characteristics of organic matter removal and the inhibitory effect on microorganism in the anaerobic process were studied on the organic loading and ammonium nitrogen concentration. The results obtained were as follows: For COD loading of $10kg/m^3$-day and five levels of ammonium nitrogen concentration ranging from 1,000 to 5,000 mg/L, organic removal efficiencies were about 81, 74, 67, 58, and 51%, and gas productions were 3,860, 3,520, 3,240, 3,020, and 2,790 ml/l-day, respectively. Average methane contents in the gas produced on COD loading of $10kg/m^3$-day was about 76%. Throughout the whole period of experiment, remaining VFA (as COD base) in the effluent was over 90% of remaining COD. This result indicated the inhibitory effect of high concentration of ammonium nitrogen through the facts that accumulated VFA was almost COD and organic removal efficiency decreased also with the increase of ammonium nitrogen. Especially, that implys which high concentration of ammonium nitrogen not only inhibits methane forming bacteria, but also acid forming bacteria.

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Effects of Essential Oils on pH, Pathogen, and Volatile Fatty Acid Content in Poultry Litter (식물성 오일을 깔짚에 처리시 pH, 병원균 및 휘발성 지방산에 미치는 영향에 대한 연구)

  • Woo-Whan Jang;In-Hag Choi
    • Journal of Environmental Science International
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    • v.32 no.2
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    • pp.139-143
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    • 2023
  • The effects of essential oils on pH, pathogens, and volatile fatty acids (VFA) in two poultry litters were investigated through a lab study. Essential oil-added poultry litters were randomly divided to two groups: control (200 g poultry litter) and Treatment (50 g thymol/Briefly, 200 g broiler litter was treated with or without 50 g thymol (Control and T1, respectively; 1 groups) and 200 g duck litter was treated with or without 50 g carvacrol (Control and T2, respectively; 2 group). Adding thymol to broiler litter increased the pH, reduced pathogens, and did not affect VFA. Interestingly, adding carvacrol slightly reduced the pH of duck litter, but had no significant effect on reducing pathogens and VFA. This difference is probably because the essential oil used and the properties of the two litters are different. In addition, pH was thought to control the odor generated from the litter, but this has not been proven. Further field studies should focus on clarifying this point.

Nutritional Evaluation of Rice with Different Processing Treatments on in vitro Rumen Fermentation Characteristics and in situ Degradation (재고미의 가공처리에 따른 in vitro, in situ 소화율 및 발효성상 평가)

  • Yang, Sung-Jae;Jung, Eun-Sang;Kim, Han-Been;Shin, Taek-Soon;Cho, Byung-Wook;Cho, Seong-Keun;Kim, Byeong-Woo;Seo, Ja-Kyeom
    • Korean Journal of Organic Agriculture
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    • v.26 no.2
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    • pp.281-296
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    • 2018
  • This study was conducted to evaluate the effect of different processing of rice on rumen fermentation in in vitro and in situ experiments. Different processing treatments (extruding, roasting, and steaming) were used in this study and all treatments were ground through a cyclone mill (Foss, Hillerød, Denmark) fitted with a 1 mm screen. Non-treated rice was considered to a control substrate. Then, all treatments were used in in vitro and in situ experiments. Total gas production and dry matter digestibility in control were lower than any other treatment at all incubation times (P<0.01). The lowest ammonia nitrogen ($NH_3-N$) concentration was observed in control among treatments at 6, 12, and 24 h incubation (P<0.01). Extruding had a highest total volatile fatty acids (VFA) concentration at 6, 12 h incubation (P<0.01) and Steaming exhibited a highest total VFA at 24 h (P<0.01). The lowest total VFA concentration was observed in control at 6, 12, and 24 h (P<0.01). In an in situ, The highest value of soluble fraction, degradation rates, effective degradability was observed in extruding (P<0.01). It was considered that feed processing increased dry matter digestibility, total VFA concentration, and decreased pH as well as $NH_3-N$ concentration indicating that processing may increase nutrient degradation of rice in the rumen.

Effects of Denitrification on Acid Production in a Two-phase Anaerobic Digestion Process (2상 혐기성 소화공정에서 탈질반응이 산생성에 미치는 영향)

  • Park, Sang-Min;Park, Noh-Back;Seo, Tae-Kyeong;Jun, Hang-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.6
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    • pp.628-636
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    • 2008
  • Anaerobic denitrification in a two phase anaerobic digestion(TPAD) process combined with biological nutrients removal (BNR) system was studied for a piggery wastewater treatment. Denitrification efficiency and the effects of the nitrified effluent on acidification was investigated by recycling the nitrified effluent to the acidogenic reactor. Recycle of the nitrified effluent to the acidogenic reactor enhanced the conversion efficiency of the influent COD into volatile fatty acids(VFAs) in the TPAD-BNR system treating the piggery wastewater. Acidification rate of the acidogenic sludge acclimated with the nitrified effluent showed 6 times higher than that acclimated without it. VFA could be used for denitrification as carbon sources, however, nitrate could enhance acidification activity in the acidogenic reactor. VFA production rate was affected on the COD/Nitrate(COD/N) ratio, however, it depended much more whether the acidogenic sludge acclimated with nitrate or not. Denitrification with the acidogenic sludge acclimated without nitrified effluent followed zero-order reaction and the reaction rate constants were in the range of 1.31$\sim$1.90 mg/L$\cdot$h. Denitrification reaction rate constants of the acidogenic sludge acclimated with nitrified effluent were 3.30 mg/L$\cdot$h that showed almost twice of them evaluated from the previous tests. The stoichiometric ratios of utilized COD to removed nitrate showed similar in both tests which were in the range of 5.1$\sim$6.4 at COD/N ratio of 10.