• Microbiology and Biotechnology Letters
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• v.20 no.3
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• pp.257-262
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• 1992

Virginiae butanolide (VB) is an autoregulator which triggers virginiamycin production in Strefltomyces virginiae. VB-C binding protein activity was investigated under various additives. The VB-C binding protein was almost fully observed in sotubte fraction (>90%) and the binding activity was optimum at pH 7.0. The VB-C binding activity was increased about 15% in 0.5 M KCI, whereas decreased about 60% in 20 mM $Mo^{6+}$. Chelating reagents (ethylenediarnine tetraacetic acid, ethyleneglycol bis(2-aminoethylether) tetraacetic acid, 8-hydroxyquinoline) and SH protecting reagents (rnercaptoethanol, dithiothreitol, thioglycerol) inhibited the VB-C binding activity about 30~55% and 3~20%, respectively. Serine protease inhibitor (phenyl methane sulfonyl fluoride), nucleotides (guanosine 5'-monophosphate, adenosine 3',5'-cyclic monophosphate), and phosphatases (alkaline, acid phosphatase) increased the VB-C binding activity about 17%, 6~20%, and 4- 13%, respectively.

• Microbiology and Biotechnology Letters
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• v.22 no.5
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• pp.459-466
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• 1994

Virginiae butanolide C(VB-C) is one of the butyrolactone autoregulators, which triggers the production of virginiamycin in Streptomyces virginiae. Streptomyces longwoodensis was selected as a test strain to investigate new VB-C functions. When 100 ng/ml of the synthetic VB-C was added into the culture at 5 hour and 0 hour, the initial production time of antibiotics and a dark blue pigment were shortened by 4~6 hours and 2~4 hours, respectively. HPLC analysis revealed the production of several new antibiotics by VB-C addition. In the SDS-PAGE analysis of the total protein from mycelium several new protein bands showed up and the amounts of certain protein bands increased in the presense of VB-C. The existence of specific VB-C binding protein was confirmed from S. longwoodensis in relation to VB-C signal transduction. These results suggest that the VB-C might have an ability to induce the production of secondary metabolites in Streptomy- ces longwoodensis.

• The Journal of Korean Society for Radiation Therapy
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• v.31 no.2
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• pp.51-63
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• 2019

Purpose: Radiation therapy for breast cancer should consider the change in breast shape due to breathing and swelling. In this study, we evaluate the benefit of using virtual bolus for IMRT of left breast cancer. Materials and methods: 10 patients with early breast cancer who received radiation therapy after breastconserving surgery compared the VMAT and IMRT plans using the virtual bolus method and without using it. The first analysis compared the V95%, HI, CI of treatment volume, Dmean, V5, V20, V30 of ipsilateral lung, and Dmean of heart in VMAT plan made using the virtual bolus method(VMAT_VB) to the plan without using it(VMAT_NoVB) in case there is no change in the breast. In IMRT, the same method was used. The second analysis compared TCP and NTCP based on each treatment plan in case there is 1cm expansion of treatment volume. Result: If there is no change in breast, V95% in VB Plan(VMAT_VB, IMRT_VB) and NoVB Plan(VMAT_NoVB, IMRT_NoVB) is all over 99% on each treatment plan. V95% in VMAT_NoVB and VMAT_VB is 99.80±0.17% and 99.75±0.12%, V95% in IMRT_NoVB and IMRT_VB is 99.67±0.26% and 99.51±0.15%. Difference of HI, CI is within 3%. OAR dose in VB plan is a little high than NoVB plan, and did not exceed guidelines. If there is 1cm change in breast, VMAT_NoVB and IMRT_NoVB are less effective for treatment. But VMAT_VB and IMRT_VB continue similar treatment effect compared in case no variation of breast. Conclusion: This study confirms the benefit of using a virtual bolus during VMAT and IMRT to compensate potential breast shape modification.

• Proceedings of the Korean Information Science Society Conference
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• 2000.04a
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• pp.259-261
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• 2000

B-ISDN의 가입자망 내에 도입된 광대역 액세스 망은 다양한 유형의 가입자들을 서비스 노드로 집선 또는 다중화하는 기능을 수행한다. 액세스 망의 ATM 연결을 동적으로 제어하기 위한 VB5.2 인터페이스의 프로토콜로는 B-ANCC가 있으며, 가입자망의 연결 및 호 처리를 위한 프로토콜로 DSS2, 국간 망에서는 B-ISUP이 표준화되어 있다. 전송 시스템의 각 구간마다 서로 다른 프로토콜들이 사용되므로 이들 프로토콜간의 연동 기능이 필요하며, 순차형 연동 방식과 병렬형 연동 방식이 제안되어 있다. 순차형 연동 방식은 VB5.2 인터페이스 프로토콜의 응답이 올 때까지 기다린 후, 국간 망 프로토콜의 연동을 진행하는 방식으로 동작하고, 병렬형 연동 방식은 VB5.2 인터페이스 프로토콜과 국간 망 프로토콜의 연동을 동시에 진행하는 방식을 사용한다. 본 논문에서는 ATM 연결등에 대한 순차형 및 병렬형 연동 방식의 시뮬레이션을 수행하여 연동 방식이 연결 설정 지연 및 완료비에 미치는 영향을 분석하였다.

• Journal of KIISE:Information Networking
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• v.27 no.2
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• pp.237-246
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• 2000

A broadband access network in B-ISDN subscriber network, plays a role of concentration or multiplexing of various subscribers into a service node. For call and connection control functions, DSS2 and B-ISUP signalling protocols are standardized for each of a subscriber side and a network node. B-BCC and B-ANCC protocol were proposed for VB5.2 interface. This interface allows dynamic control of ATM connections at the broadband access network. To establish an end-to-end ATM connection, interworking functions between VB5.2 interface's connection control protocol and the signalling protocol, are performed at the service node. A sequential interworking model and a parallel interworking model were proposed for these interworking functions. The sequential interworking model recommended by ITU-T causes an overall connection setup delay by introducing the access network, because this model proceeds the signalling protocol of network node after the response of VB5.2 interface protocol. On the other hand, the parallel interworking model proceeds the signalling protocol and the VB5.2 interface protocol simultaneously. The simultaneity of the parallel interworking model minimizes the overall connection setup delay. In this paper, we analyze and simulate above two interworking models with B-BCC or B-ANCC protocol in terms of a connection setup delay and completion ratio.

• Journal of Korea Society of Industrial Information Systems
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• v.5 no.2
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• pp.22-31
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• 2000

The VB5.2 interface of B-lSDN, that is located between an access network and a service node, allows dynamic allocation and release of ATM resources. In this paper, we propose the B-ANCC protocol of the VB5.2 interface to minimize the overall connection setup delay by introducing the access network. The B-ANCC protocol enhances the B-BCC protocol and adopts a parallel interworking function with signaling protocols in the service node. To confirm the correctness of the proposed B-ANCC protocol, we validate it using the automated validation tool, SPIN. We analyze and simulate the sequential interworking model based on the B-BCC protocol and the parallel interworking model based on the B-ANCC protocol, in terms of a connection setup delay and a completion ratio. It is shown that our proposed parallel interworking model with B-ANCC reduces a setup delay and improves a completion ratio compared to the sequential interworking model with B-BCC.

• Journal of Trauma and Injury
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• v.34 no.1
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• pp.31-38
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• 2021

Purpose: Traumatic vertebral injuries have a prevalence of 4-5% at level I centers. Studies have demonstrated that isolated thoracolumbar transverse process fractures (iTPF) rarely require brace or surgical interventions. We hypothesized that similarly isolated thoracolumbar spinous process fractures (iSPF) would have less need for bracing and operative interventions than SPFs with associated vertebral body (VB) fractures (SPF+VB). We performed a similar analysis for iTPF compared to transverse process fractures associated with VB injury (TPF+VB). Methods: In this single-center, retrospective study from 2012 to 2016, patients were classified into iSPF, SPF+VB, iTPF, and TPF+VB groups. Data including the fracture pattern, neurologic deficits, and operative intervention were obtained. The primary outcome studied was the need for bracing and/or surgery. A statistical analysis was conducted. Results: Of 98 patients with spinous process fractures, 21 had iSPF and 77 had SPF+VB. No iSPF patients underwent surgery, whereas 24 (31.17%) SPF+VB patients did undergo surgery (p=0.012). In the iSPF group, three patients (15%) received braces only for comfort, whereas 37 (48.68%) of the SPF+VB group required bracing (p=0.058). Of 474 patients with transverse process fractures, 335 had iTPF and 139 had TPF+VB. No iTPF patients underwent surgery, whereas 28 (20.14%) TPF+VB patients did (p≤0.001). Of the iTPF patients, six (1.86%) were recommended to receive braces only for comfort, while 68 (50.75%) of the TPF+VB patients required bracing (p<0.001). Conclusions: No patients with iSPF or iTPF required surgical intervention, and bracing was recommended to patients in these groups for comfort only. It appears that these injures may be safely managed without interventions, calling into question the need for spine consultation.

• Animal Bioscience
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• v.34 no.4
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• pp.701-713
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• 2021

Objective: The present work was undertaken to evaluate the effects of storage time, choline chloride, and high concentrations of Cu and Zn on the kinetic behavior of vitamin degradation during storage in two vitamin premixes and four vitamin-trace mineral (VTM) premixes. Methods: Two vitamin premixes (with or without 160,000 mg/kg of choline) were stored at 25℃ and 60% humidity. Besides, four VTM premixes were used to evaluate the effects of choline (0 vs 40,000 mg/kg) and trace minerals (low CuSO4+ZnO vs high CuSO4+ZnO) on vitamin stability in VTM premixes stored in room, and the VTM premixes were stored in room temperature at 22℃. Subsamples from each vitamin and VTM premix were collected at 0, 1, 2, 3, 6, and 12 months. The retention of vitamin A (VA), vitamin D3 (VD3), vitamin E (VE), vitamin K3 (VK3), vitamin B1 (VB1), vitamin B2 (VB2), vitamin B3 (VB3), vitamin B5 (VB5), and vitamin B6 (VB6) in vitamin premixes and VTM premixes during storage was determined. The stability of vitamins in vitamin premixes and VTM premixes was determined and reported as the residual vitamin activity (% of initial) at each sampling point. Results: The effect of choline on VK3 retention was significant in vitamin premixes (p<0.05). The negative effect of storage time was significant for the retentions of VD3, VK3, VB1, VB2, VB5, and VB6 in vitamin premix (p<0.05). For VTM premixes, negative effect of storage time was significant (p<0.05) for the losses of vitamin in VTM premixes. Choline and high concentrations of Cu and Zn significantly increased VA, VK3, VB1, and VB2 loss during storage (p<0.05). The supplementation of high concentrations of Cu and Zn significantly decreased the concentrations of VD3 and VB6 (p<0.05) in VTM premixes at extended storage time. Conclusion: The maximum vitamin stability was detected in vitamin and VTM premixes containing no choline or excess Cu and Zn. The results indicated that extended storage time increased degradation of vitamin in vitamin or VTM premixes. These results may provide useful information for vitamin and VTM premixes to improve the knowledge of vitamin in terms of its stability.

• Biomolecules & Therapeutics
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• v.31 no.1
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• pp.127-138
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• 2023

Glycogen synthase kinase-3β (GSK-3β) is an important serine/threonine kinase that implicates in multiple cellular processes and links with the neurodegenerative diseases including Alzheimer's disease (AD). In this study, structure-based virtual screening was performed to search database for compounds targeting GSK-3β from Enamine's screening collection. Of the top-ranked compounds, 7 primary hits underwent a luminescent kinase assay and a cell assay using human neuroblastoma SH-SY5Y cells expressing Tau repeat domain (Tau_RD) with pro-aggregant mutation ΔK280. In the kinase assay for these 7 compounds, residual GSK-3β activities ranged from 36.1% to 90.0% were detected at the IC₅₀ of SB-216763. In the cell assay, only compounds VB-030 and VB-037 reduced Tau aggregation in SH-SY5Y cells expressing ΔK280 Tau_RD-DsRed folding reporter. In SH-SY5Y cells expressing ΔK280 Tau_RD, neither VB-030 nor VB-037 increased expression of GSK-3α Ser21 or GSK-3β Ser9. Among extracellular signal-regulated kinase (ERK), AKT serine/threonine kinase 1 (AKT), mitogen-activated protein kinase 14 (P38) and mitogenactivated protein kinase 8 (JNK) which modulate Tau phosphorylation, VB-037 attenuated active phosphorylation of P38 Thr180/ Tyr182, whereas VB-030 had no effect on the phosphorylation status of ERK, AKT, P38 or JNK. However, both VB-030 and VB-037 reduced endogenous Tau phosphorylation at Ser202, Thr231, Ser396 and Ser404 in neuronally differentiated SH-SY5Y expressing ΔK280 Tau_RD. In addition, VB-030 and VB-037 further improved neuronal survival and/or neurite length and branch in mouse hippocampal primary culture under Tau cytotoxicity. Overall, through inhibiting GSK-3β kinase activity and/or p-P38 (Thr180/Tyr182), both compounds may serve as promising candidates to reduce Tau aggregation/cytotoxicity for AD treatment.

• Journal of Korea Multimedia Society
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• v.4 no.5
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• pp.455-464
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• 2001

For future multimedia services, one of the most crucial issues is building an access network that can accommodate multimedia services in subscriber network. The VB5.2 interface of B-ISDN located between an access network and a service node allows dynamic allocation and release of ATM resources. The SG 13 of ITU-T is standardizing the B-BCC protocol, which is sequentially interworked with signaling protocols in the service node. To minimize a connection setup delay of the sequential interworking mode, we proposed the parallel interworking model in which the SN executes simultaneously the connection control protocol of VB5.2 interface and signaling protocol. We simulate two interworking models in terms of a connection setup delay and a completion ratio. The results of simulation show that our proposed parallel interworking model for the VB5.2 interface reduces the setup delay and has the similar completion ratio compared to the sequential interworking model. however, the connection setup delay of parallel interworking model becomes about seven tenths of that of the sequential interworking model and the improvement become larger as the arrival rate increased.