• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.143-147
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• 2005

The non-pathogenic, non-glycopeptide-producing actinomycete Streptomyces coelicolor carries a cluster of seven genes (vanSRJKHAX) that confers inducible, high-level resistance to vancomycin. The van genes are organised into four transcription units, vanRS, vanJ, vanK and vanHAX, and these transcripts are induced by vancomycin in a vanR-dependent manner. vanHAX are orthologuous to genes found in vancomycin resistant enterococci that encode enzymes predicted to reprogramme peptidoglycan biosynthesis such that cell wall precursors terminate in D-Ala-D-Lac, rather than D-Ala-D-Ala. vanR and vanS encode a two-component signal transduction system that mediates transcriptional induction of the seven van genes. vanJ and vanK are novel genes that have no counterpart in previously characterised vancomycin-resistance clusters from pathogens. VanK is essential for vancomycin resistance in S. coelicolor and it is required for adding Gly branch to stem peptides terminating D-Ala-D-Lac. Because VanK can recognise D-Lac-containing precursors but the constitutively expressed femX enzyme, encoded elsewhere on the chromosome, cannot recognize D-Lac-containing precursors as a substrate, vancomycin-induced expression of VanHAX in a vanK mutant is lethal. Further, femX null mutants are viable in the presence of glycopeptide antibiotics but die in their absence. Bioassay using vanJp-neo fusion reporter system also showed that all identified inducers for van genes expression were glycopeptide antibiotics, but teicoplanin, a membrane-anchored glycopeptide, failed to act as an inducer.

• Communications of Mathematical Education
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• v.37 no.3
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• pp.483-495
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• 2023

In his book "Exercitationum Mathematicalarum," a 17th-century mathematician van Schooten proposed linkages for drawing parabola, ellipse, and hyperbola. The linkages proposed by van Schooten can be used in action-based mathematics education and as a material for using mathematical history in school mathematics. In particular, students are not provided with the opportunity to learn by manipulating the quadratic curves in the high school curriculum, so van Schooten's linkages can be used for school mathematics. To this end, a method of implementing van Schooten's linkage in a dynamic geometry environment was presented, and proved that the traces of the figure drawn using van Schooten's linkage were parabola, ellipse, and hyperbola.

• Korean Journal of Clinical Laboratory Science
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• v.46 no.2
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• pp.64-67
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• 2014

Vancomycin-resistant Enterococci (VRE) are a leading cause of a nosocomial infection. While seven glycopeptide resistance genotypes have been found in Enterococci, vanA and vanB are the most common resistance genotypes. Aims of this study were to detect antibiotic susceptibilities of 23 Enterococcus spp, which broke out in a university hospital by the disk diffusion test, to investigate specific genes of vanA and vanB by conventional and real-time PCR. PCR for vanA and vanB was performed on 23 Enterococci, all 23 were positive for vanA type. This study reports the validation of a simple and rapid VRE detection method that can be easily incorporated into the daily routine of a clinical laboratory. Early detection of VRE strains, including those with susceptibility to Vancomycin, is of paramount clinical importance, as it allows a rapid initiation of strict infection control practices as well as a therapeutic guidance for a confirmed infection. The real-time PCR method is a rapid technique to detect vanA in Enterococci. It is simple and reliable for the rapid characterization of VRE.

• Biomedical Science Letters
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• v.26 no.3
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• pp.149-156
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• 2020

We developed a simple and rapid method for detecting vancomycin resistance genes, such as vanA and vanB, using loop-mediated isothermal amplification (LAMP). To identify not only vancomycin resistance genes, but also the genus Enterococcus, primers were designed for vanA, vanB, and 16S rRNA. Screening for vancomycin susceptibility in Enterococcus was performed using Etest (bioMérieux Inc). The results of the LAMP assay were compared to those of real-time RT-PCR. The optimal conditions for the LAMP assay were 65℃ for 60 min. The detection limits of the LAMP assay for vanA, and vanB were 2 × 10² copies/reaction. Compared to RT-PCR, the sensitivities and specificities of LAMP for 16S rRNA, vanA, and vanB were 100/100%, 100/100%, and 100/100%, respectively. The vanA genotype-vanB phenotype accounted for 57.5% (46/80) of the vancomycin-resistant Enterococci samples collected from 2016 to 2019. In conclusion, the LAMP assay developed in this study showed high sensitivity and specificity for vancomycin-resistant genes. Moreover, due to the simplicity and rapidity of the LAMP assay, its use can be very useful in clinical microbiology laboratories.

• The Mathematical Education
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• v.39 no.2
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• pp.151-166
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• 2000

The purpose of this study is to understand what two models of SOLO taxonomy and van Hiele theory suggest and find out what relation there is between the category system of the SOLO taxonomy and the thinking level of the van Hiele theory. The van Hiele theory describes in line of ranking level so that it may increase the teaching effects by putting together a class, which takes into consideration the students thoughts. The SOLO taxonomy focused on the response mode of the students rather than the thinking level or the developmental stage of them to pursuit the method that can describe the students understanding in depth quality-wise. Although the SOLO taxonomy and the van Hiele model seem to have different form and character from outside in terms of their goals, a closer examination reveals that the two stances have much in common and that the models are complementary. Although the van Hiele placed more focus on the thoughts, because the conclusion was based on the students responses, the van Hiele theory can be interpreted within the structure identified in the SOLO model. In this study, we have tried to understand how the response structure form the SOLO taxonomy and the thinking level of the van Hiele theory are related, based on the studies of Pegg and Davery1998). If you briefly look at them, there are following corresponding relation between the SOLO taxonomy and the van Hiele theory. a) The relational level(R) in iconic moe is van Hiele level 1. b) The multisturctural level(M$_2$) in the second cycle of concrete-symbolic mode is van Hiel level 2. c) The relation level(R$_2$) in the second cycle of concrete-symbolic mode is van Hiele level 3. d) The unistructural level(U$_2$) in the second cycle of formal mode is van Hiele level 4. e) The postformal mode is van Hiele levle 5. Though it would be difficult to conclude that these correspondences were perfectly done, if you look at their relation, you can see that the learning process of the students were not carried out uniformly. Therefore, by studying the students response structure, using the SOLO taxonomy, and identifying the learning cycle and understand the geometrical concept more in depth.

• The Journal of the Korea institute of electronic communication sciences
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• v.11 no.1
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• pp.87-92
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• 2016

Van der Pol's oscillators is non-conservative oscillator that having nonlinear damping phenomena. The energy of its system is dissipative at a high amplitude whereas its system creates the energy at low amplitude. In order to identify another behaviors in the Van der Pol oscillator, the periodic external force applied in the Van der Pol oscillator. This paper confirms the pattern of variation for the limit cycle according to parameter variation in order to identify another behaviors in the Van der Pol oscillator.

• Biomedical Science Letters
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• v.5 no.1
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• pp.95-100
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• 1999

It is generally difficult, time-consuming, and expensive for the clinical laboratory to detect vancomycin resistant enterococci (VRE). The aim of this study was to develop and evaluate the multiplex polymerase chain reaction (PCR) assay system as a diagnostic tool for the rapid detection of VRE from clinical samples and/or for the identification of VRE from the bacterial strains isolated from clinical specimens. Specific primers, designed from the nucleotide sequences respectively encoding the vanA, vanB, vanC-1, vanC-2/3 genes in enterococci, were coupled in a multiplex PCR assay system. With this multiplex PCR assay system, we investigated the incidence rates and types of VRE isolated from clinical samples. A total of 75 strains of enterococci were isolated in 3 general hospitals in Korea. Of these isolates, 36 strains showed a pattern of high-level vancomycin resistance which associated with vanA gene, whereas 18 strains showed low-level vancomycin resistance associated with vanC-1 or vanC-2/3 gene. Thus, multiplex PCR assay method established in this study could be applied for the rapid detection of VRE.

• 정보화사회
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• s.3
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• pp.44-47
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• 1988

지난 해 5월 정부가 그룹 VAN을 허용함에 따라 그룹사 정보처리 네트웍화의 길이 열렸다. 이로써 우리 나라에도 민간 VAN이 시작되었다. 민간 VAN은 이미 선진 각국의 주요 기업에 의해 산업화되어 급성장하고 있고, 국내에서도 근래들어 그룹사들에 의해 경영정보 공동활용과 새로운 유망 사업 분야로서 구상되기에 이르렀다. 이렇게 도입 욕구가 팽배된 적절한 시기에 민간 VAN이 허용됨에 따라 럭키금성을 비롯한 6개 그룹사가 이에 참여 하고 있는 가운데 제도상.기술상의 제반 문제점들이 도출되고 있다. 그룹 VAN 진출 기업들을 회원으로 하고 있는 본 협회는 국내 민간 VAN 사업의 활성화를 위하여 구랍 28일 문제점들의 시정을 정책 당국과 공중통신사업자, 그리고 진출기업에 건의 했다. 그 내용을 전개한다.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.4
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• pp.273-278
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• 2008

This study was carried out in order to test unsaturated hydraulic conductivity estimation of van Genuchten's and Campbell's models using one-step outflow method through Tempe pressure cell. The undisturbed soil cores (columns) were taken from Ap1, B1 and C horizons of Songjeong series (the fine loamy, mesic family of Typic Hapludults). After the saturated hydraulic conductivity Ks of the cores was determined by constant head method, water outflow rate and retentivity of cores were measured in Tempe pressure cell. Fitted curves by models accorded to measured data except for both end of pressure range. In near-saturated condition, measured water retention characteristics showed a relatively better fitness with Campbell's model than van Genuchten's. The soil unsaturated conductivity estimated by Campbell's model was higher than by van Genuchten's. In Ap1 and B1 horizon, the soil unsaturated conductivities obtained by one-step outflow method went between van Genuchten's and Campbell's hydraulic functions, slightly closer to van Genuchten's. In C horizon, van Genuchten's model had better fitness with the one-step outflow data. Consequently, van Genuchten's model generally had better fitness with measured hydraulic conductivity than Campbell's model at the soil water potential range of -10~-75 kPa, especially in C1 horizon. In near-saturated condition, Campbell's model could be thought as relatively accurate hydraulic model, because of the better fitness of Campbell's model with soil water retention data than van Genuchten's model.

• Korean Journal of Veterinary Research
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• v.43 no.1
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• pp.103-112
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• 2003

A multiplex PCR assay, which allows simultaneous detection of vancomycin resistant genotypes and Enterococcus species-specific genes, was developed. Vancomycin resistant enterococci (VRE) from chickens and humans could be detected for vanA, vanB, vanC-1, vanC-2, $ddl_{E.faecium}$ and $ddl_{E.faecalis}$ by multiplex PCR. Eight isolates of VRE from humans (n=11) had $ddl_{E.faecium}$ and vanA, and 3 isolates of the VRE had $ddl_{E.faecium}$ and vanB. One isolate of VRE from chickens (n=6) had $ddl_{E.faecium}$ and vanA, and 5 isolates of the VRE had only vanA. E. faecium, E. faecalis, E. gallinarum and E. casseliflavus were also confirmed for the species-specific gene by multiplex PCR. This multiplex PCR could detect E. faecium, E. faecalis, E. gallinarum, E. casseliflavus, vanA, vanB, vanC-1 and vanC-2, simultaneously. The PCR assay established in the present study can be an alternative to time-consuming biochemical tests and antibiotic susceptibility tests of Enterococcus spp.