• Title/Summary/Keyword: V2C

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First report of a bacteriovorus nematode, Diploscapter coronatus (Cobb, 1893) Cobb, 1913 (Rhabditida: Diploscapteridae), in Korea

  • Kang, Heonil;Eun, Geun;Ha, Jihye;Park, Namsook;Choi, Insoo;Kim, Donggeun
    • Journal of Species Research
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    • v.6 no.2
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    • pp.177-180
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    • 2017
  • Diploscapter coronatus (Cobb, 1893) Cobb, 1913 was first reported on oriental melon (Cucumis melo vars. makuwa Makino) roots from plastic film houses, Gyeongsangbuk-do, Andong, Korea (latitude $N36^{\circ}33^{\prime}$. longitude $E128^{\circ}29^{\prime}$). Diploscapter coronatus is most similar to D. pachys. Species in the genus Diploscapter have a visibly annulated cuticle. The stoma of D. coronatus is 1.5 times longer than the lip region width. The pharyngeal corpus is clearly separated from the isthmus and the vulva is situated around the mid-body. However, it differed by higher ratio of "a", filiform tail and had relatively blunt labial hooks. The Korean population is well matched and within the range of D. coronatus as described from Iran and Ethiopia in de Man's of L, b, c, c', and V. Female length, however, varied between populations: the Ethiopia population female length is longer than in the Korea population (396.4 vs 427.0) while females in the Iran population are smaller than in the Korea population (396.4 vs 350.0). Nematode size may vary due to environmental conditions such as food sources. The position of excretory pores in the Korean population were shorter 53.9-72.5 than in other populations (67-82 and 70-89). Males were uncommon. Males in the Korean population are smaller than females but larger(356.0) than males in the Indian population (306.0).

Dissolved Oxygen Budget in Floating Net Cage of Fish Farm at the Coastal Area -In case of yellow tail farm in Konli-Do- (해산 어류 양식장 가두리의 DO수지 -곤리도 방어 양식장의 경우-)

  • KIM Yong Sool
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.21 no.1
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    • pp.30-34
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    • 1988
  • In roastal fish farms the farmers, especialy engaging in dealing with the floating cage culture, going to know about relationships between holding capacity and water quality in cage. Some of water quality managers and specialists studing physiological ecology understand that the key of water quality management concerned fish farming is budget of dissolved oxygen. This paper deals with oxygen budget in floating cage of the yellow tail farms at southern coastal area in Korea. The sampling station is located at Konli-Do fish farm near Chungmu, and the data is collected for 24 hours from 3:00 p.m. 8th September 1987. In result, the needed oxygen coming after the consumption by the rearing fish had been supplied with the tide current exchange, the sum of oxygen produced by phytoplankton photosynthesis and diffused from atmosphere are no more that $43\%$ for the needs of sea water consumption included respiration of planktons and decomposition of organic matters. The optimum holding capacity of cage is possible to compute with the calculation of minimum diurnal water exchange rate $[Qin{\cdot}V^{-1}\;(C-\bar{c})]$ through net mesh of cage.

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Monoclonal Antibody-Based Indirect-ELISA for Early Detection and Diagnosis of Epiphytic Didymella bryoniae in Cucurbits

  • Lee, Sun-Cheol;Han, Ki-Soo;Lee, Jung-Han;Kim, Dong-Kil;Kim, Hee-Kyu
    • The Plant Pathology Journal
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    • v.19 no.5
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    • pp.260-265
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    • 2003
  • Gummy stem blight caused by Didymella bryoniae occurs exclusively in cucurbits. This fungus has been known not to produce its pycnidium in vitro unless irradiated. In this study, cultural conditions for the mass-production of pycnidiospore by Metal Halide (MH) lamp irradiation were maximized. The mycelia were cultured at $26^{\circ}C$ on PDA for 2 days under dark condition, and then the plate was illuminated with MH lamp continuously for 3-4 days at $26^{\circ}C$. Results show that a great number of pycnidia were simultaneously formed. The pycnidiospores produced were then used as immunogen. Fusions of myeloma cell (v-653) with splenocytes from immunized mice were carried out. Two hybridoma cell lines that recognized the immunogen D. bryoniae were obtained. One monoclonal antibody (MAb), Dbl, recognized the supernatant while another MAb, Db15, recognized the spore. Two clones were selected which were used to produce ascite fluid of the two MAb, Dbl and Db15, the immunotypes of which were identified as IgG1 and IgG2b, respectively. Titers of MAb Dbl and MAb Db15 were measured and the absorbance exceeded 0.5 even at a $10^{-5}$ dilution. The MAbs reacted positively with D. bryoniae but none reacted with other viral isolates, Cucumber mosaic virus and Cucumber green mottle mosaic virus. Sensitivity of MAb was precise enough to detect spore concentration as low as $10^{-3}$/well by indirect ELISA. Characterization of the MAbs Dbl, Db15 antigen by heat and protease treatments, which suggests that the epitope recognized by these two MAbs was glycoprotein.

The Development of Estimation Model for Iron Compound Originated from Anaerobic Microbial Corrosion (혐기성 미생물부식에 의해 생성되는 철화합물 추정식 개발)

  • Jeon, Seok-Jun
    • Journal of Korean Society on Water Environment
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    • v.18 no.4
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    • pp.379-386
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    • 2002
  • In this study, estimation model for iron compound originated from upflow, anaerobic fixed bed reactor, which treats sewage domestic wastewater, was developed. The estimation model was formulated by a mathematical expression which was based on the mass balance. Below the HRT of 60 minute, sulfide concentration combining with iron $FeS_2$ is the highest because the maximum sulfate consumption rate $V_{maxS}$ and half-saturation constant of sulfate $K_{mS}$ exert an important effect on the estimation model as temperature was increased. But increment of $FeS_2$ concentration is weakened above the HRT of 60 minutes and represent the lowest value at the HRT of 108 minutes. It implies that liquid phase distribution ratio of sulfide ${\alpha}r$ becomes lower as temperature was increased. While phosphorus concentration combining with iron $Fe_3(PO_4)_3$ is increased as HRT and temperature are increased, which is affected by phosphorus removal rate constant $k_p$. As the result of estimating the iron concentrations of corrosion by the model, the concentration of iron corrosion is higher than any other at the HRT of 108 minute and $20^{\circ}C$. The predicted values were compared with measured ones at different HRT(13.5, 27, 54, 108 min) and temperature(20, 25, $30^{\circ}C$). The experimental data could be fitted with the simulated curves. Therefore, the mathematical expression could be applicable to design full-scale wastewater treatment plants.

Deposition of Indium Tin Oxide films on Polycarbonate substrates by Ion-Assisted deposition (IAD)

  • Cho, Jn-sik;Han, Young-Gun;Park, Sung-Chang;Yoon, Ki-Hyun;Koh, Seok-Keun
    • Proceedings of the Korean Vacuum Society Conference
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    • 1999.07a
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    • pp.98-98
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    • 1999
  • Highly transparent and conducting tin-doped indium oxide (ITO) films were deposited on polycarbonate substrate by ion-assited deposition. Low substrate temperature (<10$0^{\circ}C$) was maintained during deposition to prevent the polycarbonate substrate from be deformed. The influence of ion beam energy, ion current density, and tin doping, on the structural, electrical and optical properties of deposited films was investigated. Indium oxide and tin-doped indium oxide (9 wt% SnO2) sources were evaporated with assisting ionized oxygen in high vacuum chamber at a pressure of 2$\times$10-5 torr and deposition temperature was varied from room temperature to 10$0^{\circ}C$. Oxygen gas was ionized and accelerated by cold hallow-cathode type ion gun at oxygen flow rate of 1 sccm(ml/min). Ion bea potential and ion current of oxygen ions was changed from 0 to 700 V and from 0.54 to 1.62 $\mu$A. The change of microstructure of deposited films was examined by XRD and SEM. The electrical resistivity and optical transmittance were measured by four-point porbe and conventional spectrophotometer. From the results of spectrophotometer, both the refractive index and the extinction coefficient were derived.

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Evaluation of Biocompatibility of Anodized and Hydrothermally Treated Pure Niobium Metal (양극산화와 열수처리한 순수 니오비움 금속의 생체활성 평가)

  • Won, Dae-Hee;Choi, Un-Jae;Lee, Min-Ho;Bae, Tae-Sung
    • Journal of Technologic Dentistry
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    • v.27 no.1
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    • pp.79-88
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    • 2005
  • This study was performed to investigate the surface properties of electrochemically oxidized pure niobium by anodic oxide and hydrothermal treatment technique. Niobium specimens of $10\times10mm$ in dimension were polished sequentially from #600, #800, #1000 emery paper. The surface pure niobium specimens were anodized in an electrolytic solution that was dissolved calcium and phosphate in water. The electrolytic voltage was set in the range of 250 V and the current density was 10 $mA/cm^2$. The specimen was hydrothermal treated in high-pressure steam at 300$^{\circ}C$ for 2 hours using an autoclave. Then, specimens were immersed in the Hanks' solution with pH 7.4 at 37$^{\circ}C$ for 30 days. The surface of specimen was characterized by scanning electron microscope(SEM), energy dispersive X-ray microanalysis(EDX), potentiostat/galvanostat test, and cytotoxicity test. The results obtained was summarized as follows; According to the result of measuring corrosion behavior at 0.9% NaCl, corrosion resistance was improved more specimens treated with anodic oxide than in hydrothermal treated ones. The multi-porous oxide layer on surface treated through anodic oxidation showed a structure that fine pores overlap one another, and the early precipitation of apatite was observed on the surface of hydrothermal treated samples. According to the result of EDX after 30 days deposition in Hanks' solution, Ca/P was 1.69 in hydrothermal treated specimens. In MTT test, specimens treated through anodic oxidation and hydrothermal treated ones showed spectrophotometer similar to that of the control group. Thus no significant difference in cytotoxicity was observed (P>0.05).

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A Design of DisplayPort AUX Channel (디스플레이포트 인터페이스의 AUX 채널 설계)

  • Cha, Seong-Bok;Yoon, Kwang-Hee;Kim, Tae-Ho;Kang, Jin-Ku
    • Journal of IKEEE
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    • v.14 no.1
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    • pp.1-7
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    • 2010
  • This paper presents an implementation of the DisplayPort AUX(Auxiliary) Channel. DisplayPort uses Main link, AUX Channel and Hot Plug Detect line to transfer the video & audio data. For isochronous transport service, source device converts to image and audio data which are to be transported through the Main Link and transports the restructured image and audio data to sink device. The AUX Channel provides link service and device service for discovering, initializing and maintaining the Main link. Hot Plug Detect line is used to confirm the connection between source device and sink device. The AUX Channel is implemented with 3315 LUTs(Look Up Table), 1466 Flip Flops and 168.782MHz max speed synthesized using Xilinx ISE 9.2i at SoC Master3.

A SCANNING ELECTRON MICROSCOPIC STUDY ON THE CHANCES OF REPARATIVE DENTIN FORMATION BY THE GLASS IONOMER CEMENT IN CATS (Glass Ionomer시멘트에 의한 상아질구조변화에 관한 주사전자현미경적 연구)

  • Park, S.K.;Woo, Y.H.;Choi, D.K.;Choi, B.B.;Park, N.S.
    • The Journal of Korean Academy of Prosthodontics
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    • v.25 no.1
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    • pp.227-242
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    • 1987
  • This study was designed to investigate the pulpal effects of the glass ionomer cement. (Lining cement, G-C Co. Japan) For this purpose, 10 cats were selected, and Class V cavities were prepared on canines of the cats. One experimental group was filled with glass ionomer cement and the other group was filled with zinc phosphate cement . (G-C Co, Japan) The animals of the experimental and control group were sacrificed at 1,2,3,4,6, weeks after the experiment. For comparison of reparative dentin formation pattern in direction of the pulpal and fractured lateral surface, each of them was observed with scanning electron microscope. The findings led to the following conclusions; 1. Reparative dentin of the glass ionomer cement and zinc phosphate cement filling groups were formed on the internal surface of dentin as the shape of hemispherical and spherical with a rough surface. 2. Some of reparative dentin of the glass ionomer cement filling group was started to form at 1 week after experiment, and at 6 weeks after experiment, it had been increased gradually in number and size. 3. Reparative dentin of zinc phosphate cement filling group was formed vigorously, however, gradually was decreased in number and size, and disappeared at 6 weeks after experiment. 4. During the formation of reparative dentin, peritubular dentins were indistinguishable. 5. The diameter of dentinal tubules of reparative dentin has been decreased, during the reparative dentin formed, and it became very irregularly at 6 weeks after experiment.

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The Expression of Porcine Adiponectin and Stearoyl Coenzyme a Desaturase Genes in Differentiating Adipocytes

  • Wang, P.H.;Ko, Y.H.;Liu, B.H.;Peng, H.M.;Lee, M.Y.;Chen, C.Y.;Li, Y.C.;Ding, S.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.5
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    • pp.588-593
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    • 2004
  • The gene expression of porcine adiponectin and stearoyl coenzyme A desaturase (SCD) was investigated in this study. The partial gene sequences for adiponectin and SCD were amplified by RT-PCR from subcutaneous adipose tissue and cloned by TA cloning techniques. Sequences of these genes were determined and found to be highly homologous to that of other species, suggesting similar function of these genes as in other species. The transcripts of these adipocyte-related genes in pig tissues were measured by Northern analysis. The transcripts for adiponectin and SCD were highly expressed in porcine subcutaneous adipose tissue; the transcripts for SCD were also barely detected in the liver, but the greatest concentrations were in the adipose tissue. In porcine stromalvascular cells (S/V cells) cultured in vitro, transcripts for adiponectin and SCD increased gradually during adipocyte differentiation. The level of adipocyte adiponectin mRNA was associated with late adipocyte differentiation, indicating the gene may not be involved in adipocyte differentiation but has great importance in porcine adipocyte functions. The SCD transcripts were not detectable until 2 d after induction of adipocyte differentiation. It was highly expressed in differentiating porcine adipocytes (2 to 10 d after the induction of adipocyte differentiation), indicating a significant role of SCD in adipocytes.

Growth and Fodder Yield of the Gliricidia sepium Provenances in Guardrow System in Dryland Farming Area in Bali, Indonesia

  • Sukanten, I.W.;Nitis, I.M.;Uchida, S.;Lana, K.;Puger, A.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.1
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    • pp.106-113
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    • 1997
  • A field experiment was carried out on a dryland farming area of southern Bali for 92 weeks, to study the growth and fodder yield of 16 provenances of Gliricidia sepium in guardrow system. The experimental design was completely randomized blocks of 16 treatments (Gliricidia sepium provenances) replicated 3 times, with 6 plants per provenance. Six provenances were from Mexico (M), four from Guatemala (G), and one each from Colombia (C), indonesia (I), Nicaragua (N), Panama (P), Costa Rica (R) and Venezuela (V). After 40 weeks establishment the gliricidia were lopped 4 times a year at 150 cm height, at 2 months intervals during the 4 month wet season and 4 month intervals during the 8 month dry season. Stem elongation varied from 21 to 81 cm, leaf retention from 39 to 240%, branch number from 12 to 35, fodder yield from 1,090 to 3,153 g DW/plant. and wood yield from 743 to 2,750 g DW/plant. Pontezuelo provenance of Colombia (C24), Belen provenance of Nicaragua (N14) and Retalhuleu provenance of Guatemala (G14) were ranked first, second and third, respectively, for stem elongation, leaf retention, fodder and wood yields, during the wet and dry seasons.