• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1371-1382
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• 2016

MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate expression of mRNAs in many biological pathways. Liver plays an important role in the feed efficiency of animals and high and low efficient cattle demonstrated different gene expression profiles by microarray. Here we report comprehensive miRNAs profiles by next-gen deep sequencing in Angus cattle divergently selected for residual feed intake (RFI) and identify miRNAs related to feed efficiency in beef cattle. Two microRNA libraries were constructed from pooled RNA extracted from livers of low and high RFI cattle, and sequenced by Illumina genome analyser. In total, 23,628,103 high quality short sequence reads were obtained and more than half of these reads were matched to the bovine genome (UMD 3.1). We identified 305 known bovine miRNAs. Bta-miR-143, bta-miR-30, bta-miR-122, bta-miR-378, and bta-let-7 were the top five most abundant miRNAs families expressed in liver, representing more than 63% of expressed miRNAs. We also identified 52 homologous miRNAs and 10 novel putative bovine-specific miRNAs, based on precursor sequence and the secondary structure and utilizing the miRBase (v. 21). We compared the miRNAs profile between high and low RFI animals and ranked the most differentially expressed bovine known miRNAs. Bovine miR-143 was the most abundant miRNA in the bovine liver and comprised 20% of total expressed mapped miRNAs. The most highly expressed miRNA in liver of mice and humans, miR-122, was the third most abundant in our cattle liver samples. We also identified 10 putative novel bovine-specific miRNA candidates. Differentially expressed miRNAs between high and low RFI cattle were identified with 18 miRNAs being up-regulated and 7 other miRNAs down-regulated in low RFI cattle. Our study has identified comprehensive miRNAs expressed in bovine liver. Some of the expressed miRNAs are novel in cattle. The differentially expressed miRNAs between high and low RFI give some insights into liver miRNAs regulating physiological pathways underlying variation in this measure of feed efficiency in bovines.

• Restorative Dentistry and Endodontics
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• v.27 no.4
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• pp.382-388
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• 2002

Compomer is composed of matrix and filler : matrix is made of the combination of resins and polycarboxylic molecules that are light-cured, and a filler is a glass component which is capable of ion-release. The resin content of compomers produces polymerization shrinkage which can adversely affect marginal adaptation. Pretreatment is a fundamental step which is treated with conditioner or primer in the use of these materials. Microleakage of restorative materials has been investigated mostly by dye penetration method. Dye penetration method was not quantitative and not measured repeatedly. Fluid filtration method, introduced and developed by Pashley's group, has been extensively used for 20 years for research purpose to understand the physiology of dentin, as well as the effects of various restorative treatments on dentin permeability. It permits quantitative, nondestructive measurment of microleakage in a longitudinal manner. The purpose of this study was to evaluate the change of dentin permeability according to the process of compomer restoration. In this study. Cl V cavities were prepared on buccal surface of thirty extracted human molars. The prepared cavities were etched by 37% phosphoric acid. The experimental teeth were randomly divided into three groups. Each group was treated with following materials Group 1 : Prime & Bond NT/Dyract AP, Group2： Single Bond/F2000 compomer, Group 3 : Syntac Single Component/Compoglass. The bonding agent and compomer were applied for each group following manufacturers information. Dentin permeability of each group was measured at each process by fluid filtration method; Step 1 : preparation(smear layer). Step 2 : etching(smear layer removal), Step 3 : applying the bonding agent, Step 4 : filling the compomer. Dentin permeability was expressed by hydraulic conductance ($\mu\textrm{l}$ min$^{-1}$cm$H_2O$$^{-1}$). The data were analysed statistically using One-way ANOVA and Sheffe's method. The results were as follows : 1. Dentin permeability differences between each process were significant except between step 1 and step 2(p<0.01). 2. Dentin permeability after removal of smear layer was highly increased(p<0.01). 3. In most case, decrease of dentin permeability was obtained by applying bonding agent(p<0.01). 4. Dentin permeability differences among the experimental groups were not significant(p>0.05). 5. None of compomers used in this study showed perfect seal at the interface.

• Journal of Sensor Science and Technology
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• v.14 no.1
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• pp.33-41
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• 2005

For more convenient electrode-electrolyte interface impedance analysis in biosensor, a stand-alone impedance measurement system is required. In our study, we developed a PC-based portable system to analyze impedance of the electrochemical cell using microprocessor. The devised system consists of signal generator, programmable amplifiers, A/D converter, low pass filter, potentiostat, I/V converter, microprocessor, and PC interface. As a microprocessor, PIC16F877 which has the processing speed of 5 MIPS was used. For data acquisition, the sampling rate at 40 k samples/sec, resolution of 12 bit is used. RS-232 with 115.2 kbps speed is used for the PC communication. The square wave was used as stimuli signal for impedance analysis and voltage-controlled current measurement method of three-electrode-method were adopted. Acquired voltage and current data are calculated to multifrequency impedance signal after Fourier transform. To evaluate the implemented system, we set up the dummy cell as equivalent circuit of which was composed of resistor, parallel circuit of capacitor and resistor connected in parallel and measured the impedance of the dummy cell; the result showed that there exist accuracy within 5 % errors and reproduction within 1 % errors compared to output of Hioki LCR tester and HP impedance analyzer as a standard product. These results imply that it is possible to analyze electrode-electrolyte interface impedance quantitatively in biosensor and to implement the more portable high speed impedance analysis system compared to existing systems.

• Journal of the Korean Chemical Society
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• v.47 no.4
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• pp.391-400
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• 2003

This study was performed the analysis of seven kinds of the hight school chemistry II textbooks based on the 6th curriculum. Particularly, inquiry activity part was analyzed by the three dimension framework which consists of inquiry content dimension, inquiry process dimension and inquiry context dimension. In the analysis of the inquiry content dimension of inquiry activities, the total number of themes in seven kinds of textbook was 212. And the number of inquiry activities in seven kinds of textbook was diverse: A textbook had 28, B textbook 25, C textbook 31, D textbook 35, E textbook 31, F textbook 29 and G textbook 33. As for the avaerage number of inquiry activities of each chapter, chapter I "Material Science" is 3.00(9.91${\%}$), chapter II "Atomic Structure and Periodic Table" 4.57(15.1${\%}$), chapter III "Chemical Bonding and Compound" 6.86(22.6${\%}$), chapter IV "State of Matter and Solution" 7.00(23.1${\%}$), chapter V "Chemical Reaction" 8.86(29.2${\%}$). For the analysis of inquiry process dimension, it follows in the order of 'observation and measuring (66.7${\%}$)', 'Interpreting data and formulating generalizations (26.5${\%}$)', 'seeing a problem and seeking ways to solve it (4.1%)', and 'building, testing and revising the theoretical model (2.7${\%}$)'. As for the analysis of the inquiry context dimension, the scientific context occupied 90.5${\%}$, the individual context 4.3${\%}$, the social context 0.9${\%}$, and the technical context 4.3${\%}$. It shows that the proportion of STS(Science-Technology-Society) related contents in inquiry activities was only 9.5${\%}$.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.7
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• pp.965-973
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• 2011

Our objective was to determine the differences in the rate and extent of dry matter digestion between barley subjected to differing agronomic variables. Two malting barley varieties, Copeland and Metcalfe were seeded at rates of 200 and 400 plants/$m^2$. Each of these varieties received nitrogen fertilizer at rates of 0, 30, 60 and 120 kg/ha, resulting in a total of 20 different barley grain samples. Samples were ground through a 6mm screen and approximately 3 g of each weighed into 50 ${\mu}m$ Dacron bags and sealed. The bags were incubated in three ruminally cannulated Holstein cattle for periods of 0, 3, 6 and 24 h. Using the data obtained from these incubations, rates of digestion were able to be predicted. The soluble fraction ranged from 0.229-0.327, the slowly degradable fraction ranged from 0.461-0.656, and the undegradable fraction ranged from 0.038-0.299. The rates of digestion ranged from 0.127-0.165 $h^{-1}$ and the effective degradability ranged from 0.527-0.757. At the Canora location, the Copeland samples which received 120 kg/ha of nitrogen fertilizer had a significantly lower (p = 0.013) soluble fraction than the rest of the samples at that location. A significant interaction (p = 0.009) was seen between the seeding rate and nitrogen fertilizer application with samples from the Canora location, as well as significant differences (p = 0.029) between nitrogen application rates in samples from the Indian head location. The rate of digestion of samples from the Indian head location differed (p = 0.020) between the two seeding rates, with samples seeded at 200 seed/$m^2$ having a slightly higher rate of degradation. Differences in the effective degradability were seen between the different nitrogen application rates with samples from both the Canora and Indian head locations, as well as an (p = 0.004) interaction between the seeding rate and nitrogen fertilizer application rate. Although there was not a clear correlation between the different variables, both nitrogen application and seeding rate did have a significant effect on the rates and extent of digestion across each of the four locations.

• KSBB Journal
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• v.4 no.1
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• pp.48-56
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• 1989

Fermentation characteristics of Jerusalem Artichoke of yeast K.fragilis CBS 1555 were investigated experimentally and quantitatively according to the change of initial sugar concentrations and initial PHs of fermentation broth. Initial sugar concentrations employed were 26, 45, 65, 105, 180, and 215g/1. And initial PHs of fermentation broth were 3, 5.5, 7 and 9. The maximum specific growth rate was observed as 0.4hr-1 at 65g/1 of initial sugar concentration. The maximum specific alcohol production rate was 1.68g/ghr at 105g/1 of initial sugar concentration Cell yield and ethanol yield represent the maximum values such as 0.14 and 0.49 respectively when the initial sugar concentration was 25g/1. The maximum of ethano1 fermentability, 97% was obtained at the initial concentrations, 26 and 45g/1. However, the maximum of total ethanol yield productivity was 2.78g/1hr when the initial concentration was 215g/1. And also the optimum PH was found 5.5 for both specific growth rate and specific alcohol production rate.

• Journal of Korean Society of Forest Science
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• v.81 no.3
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• pp.263-272
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• 1992

In a torrential river, the flow of debris forms deposits in the river bed, which show the characteristics of the channel bed movement in the watershed. The annual rings of the trees, in the natural evenaged forests on the deposits, indicate when each deposit occurred. Based on the topographical and vegetational indicators on the sediment of Yongcheon and Yeounae rivers, the movement occurrence years were estimated. 1. The cross sectional shapes of deposits in torrential river are in tiers and even-aged forests tend to establish on each tier. 2. Generally the older the forest age is, the higher the height of step from the lowest base tend to become, which indicates discrete movement in magnitude and frequency. 3. The ages of trees indicate the year when deposition occurred, and so may be useful as plant indicator to get spatial-temporal information of deposits. 4. The deposits volumes(F. V.) were dependent on the age distribution of deposits in length, average width and average height. And the average width and the average height of deposits were increasing with the age.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.3
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• pp.233-238
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• 1990

Weedy rice(red rice) collected in Korea were crossed with the Japonica, Javanica and Indica tester cultivars, and also crossed among those collected lines. Their seed fertility was counted and refered to their cross affinities. Some tester cultivars such as, Suweongjo, Malagkit Sinaguing and wx 817 which were regarded as belong to the Japonica, Javanica and Indica respectively, showed the typical tendencies of hybrid sterility when they were crossed each other. But, other testers such as Kwanak, Tapaiko, Belle Patna and Tarichaosun showed some differencies from those typical patterns, implying some varigations within a ecotype. The hybrids of weedy rice crossed to the Japonica testers showed higher fertility with a few semisterilities. While the crosses of weedy rice to the Javanicas or Indicas showed high sterility implying closer affinity relationships of weedy rice to the Japonicas. Even those weedy rice lines such as Samsanmyeon 8 and Sanchungaengmi B which showed semi-sterility when they were crossed to the Japonica testers showed high sterility when they were crossed to both Javanicas and Indicas. The hybrids among weedy rice lines showed high fertility raning 69% to 96%. Even those weedy rice lines which showed semi-sterility in the crosses with Japonica testers, showed high-fertility when they were crossed with those weedy rice lines. Though the amylose content, Alkali Digestive Value, Phenol reaction and grain shapes of theose weedy rice lines showed divers variations, the cross affinities of them showed closer phylogenie relationship to the Japonicas than to Japvanicas or Indicas. But the genetic influinces of Indicas to those weedy rices were not ignorable.

• The Korean Journal of Ecology
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• v.16 no.3
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• pp.239-259
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• 1993

The vegetation types and distributional characteristics of the forest vegetation on Mt. Ilwol were investigated by the Z-M school method. Geographical distribution of the vegetation was a central/montane(CEMO) type of the Korean Peninsula subtype. The forest was classified into five vegetation units: Artemisio-Quercetum mongolicae (Artemisio-Quercetum typicum subass. nov., Artemsio-Quercetum pinetosum dens~florae subass. nov. ). Lychno-Quercetum mongolicae (Lychno-Quercetum Calamagrostetosum arundinaceae subass. nov.. Lychno-Quercetum ligularetosum f~scherii subass. nov.), Fraxinus rhynchophylla community, Quercus variabilis community and Larix leptolepis afforestation. Species with percentage constance degree more than 61% are Quercus mongolica (89.5%, V), Lespedeza maximowiczii (73.7%, IV), Isodon japonicus(73.7%, IV), Fraxinus rhynchophylla (68.%, IV), Arlemisia stolonifera (65, 8%, IV), etc. Artemisio-Quercetum mongolicae was chiefly distributed on the ridge lines of low altitudes (570~700m) and on the ground with exposed rocks in sloping surface (800~1, 000m). Both Fraxinus sievoldiana and Rhododendrou schlippenbachii of the Artemisio-Quercetum mongolicae showed a predominant condition all over the subtree and shrub layers. Of Artemisio-Quercetum pinetosum densifloraes, Pinus densiflora becomes powerful over the tree layer and, on the other hand, Quercus mongolica over the subtree layer. Lychno-Quercetum mongolicae was distributed somewhat on the humid region of the high altitude area (840~1.200m). Fraxinus rhynchophylla community was chiefly distributed in the vicinity of the ravines. Quereus rariabilis community was distributed at subxeric slope on southeast side of the mountain. Larix leptolepis has been reforested at the ecotone between Artemisio-Quercetum mongolicae and Lychno-Quercetum mongolicae. Percentage cover degrees of the subtree layer In Lychno-Quercetum mongolicae, Quereus rariabilis community and Larix leptolepis afforestation were relatively low, 15.3%, 5% and 2.5%, respectively.The contents of moisutre and organic matter in the soil were much higher in Luchno-Quercetum monogolicae and Larix leptolepis afforestaion than in the other communities. Contents o Ca, Mg, K and the available phosphorus were relaively high compared with forests of the other area. This result seems to be influenced by a forest fire. As the altitude increases, the moisure content in the forest soil also increases.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.4
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• pp.431-436
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• 2013

Bone homeostasis is maintained by co-ordination of bone-resorbing osteoclasts and bone-forming osteoblasts. Imbalance between osteoclasts and osteoblasts leads to many bone diseases such as osteoporosis, rheumatoid arthritis. Taxillus chinensis is a herb that has been widely used to improve bone health. However, the effect and mechanism of Taxillus chinensis extract on osteoclast differentiation and bone resportion has been unknown. Thus, We investigated the effect of Taxillus chinensis on expression of receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation and bone resorption. Also, the action of Taxillus chinensis on mechanisms relating to osteoclast differentiation was studied. In this results, we identified that Taxillus chinensis significantly inhibited RANKL-induced osteoclast differentiation and bone resportion. Moreover, Taxillus chinensis was suppressed the activation of NF-${\kappa}B$ in bone marrow macrophage treated RANKL and M-CSF. Taxillus chinensis was down-regulated the mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP). The cell adhesion-related molecules such as integrin ${\alpha}v$ and integrin ${\beta}3$, and the filamentous actin (F-actin) rings of mature osteoclasts-related molecules such as dendritic cell-specific transmembrane preotein (DC-STAMP) and cathepsin K are also suppressed. Taken together, these results indicated that Taxillus chinensis will be a good candidate to treat osteoclast-mediated bone diseases.