• Title/Summary/Keyword: V$_{}$ 50/

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Effect of Buffalo Follicular Fluid Alone and in Combination with PMSG and M199 on in vitro Buffalo Oocyte Maturation

  • Gupta, P.S.P.;Nandi, S.;Ravindranatha, B.M.;Sarma, P.V.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.5
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    • pp.693-696
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    • 2001
  • The effect of replacement of in vitro maturation medium completely with the buffalo follicular fluid (buFF) on in vitro oocyte maturation of buffalo oocytes was studied. 5 to 8 buffalo cumulus oocyte complexes were cultured in a single drop with each of the eight media studied i.e., M199+steer serum (10% v/v), M199+steer serum (10% v/v)+PMSG, M199+buFF (10% v/v), M199+buFF (10% v/v)+PMSG, M199+buFF (50% v/v), M199+buFF (50% v/v)+ PMSG, buFF (100%) and buFF+PMSG at $39^{\circ}C$ and 5% $CO_2$ in air for 24 h. Supplementation of M199 with Steer serum alone resulted in IVM rate of 35% only. When the above medium was supplemented with PMSG, the maturation rate rallied to 82%. Significant increase in the maturation rates were observed when M199 was supplemented with increasing levels of buFF. A further increase in the maturation rate was also obtained when PMSG was incorporated into the medium of M199 supplemented with buFF. The rate of maturation was to the tune of 91% when oocytes were matured in buFF alone which was increased non significantly on the addition of PMSG. Highest maturation rate (97%) obtained with M199+buFF (50%v/v)+PMSG did not differ significantly from that obtained by either M199+buFF (10%v/v)+PMSG or buFF+PMSG. It is suggested that buFF alone without any supplementation can form the effective in vitro maturation medium for buffalo oocytes.

Determination of Putrescine, Spermidine and Spermine in urine by HPLC (HPLC법에 의한 뇨 중의 Putrescine, Spermidine 및 Spermine의 정량)

  • Shin, Tae-Yong;Lee, Seok-Bong;Kim, Hyung-Min
    • Analytical Science and Technology
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    • v.10 no.6
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    • pp.445-452
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    • 1997
  • A simple and sensitive high performance liquid chromatography for analysis of Putrescine, Spermidine and Spermine in urine is described. The benzoyl chloride derivatives of Putrescine, Spermidine and Spermine are separated on a ${\mu}$ Bondapak $C_{18}$ reverse-phase column with methanol/$H_2O$(50/50v/v) as the mobile phase. The results show that the mean level of polyamines in uterine cancer patients urine is much higher than in normal human urine.

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Development of a Compact 50 kV, 10 kW Transformer for High Frequency Switch Mode (50 kV, 10kW 소형 고주파 전원장치 변압기 개발)

  • Son, Y.G.;Oh, J.S.;Jang, S.D.;Cho, M.H.
    • Proceedings of the KIEE Conference
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    • 1998.07f
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    • pp.2027-2029
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    • 1998
  • We have developed a compact high frequency switch mode converter (HFSMC) power supply with DC 50 kV output voltage and 10 kW output power. Since the inverter circuit uses 25 kHz rescharging scheme, the current is flowing with t kHz. For the efficient output voltage transform performed a simple design process using the transformer principle and the commercial specification. For the DC 50 kV output, we employed 7 pa windings of secondary coils for the series stac connection of the output with full-bridge rectifi In this paper the design detail and the test re the high frequency transformer together with HFSMC power supply are presented.

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An Analysis of Fast Critical Experiments Using JEF-1-Based 50-Group Constant Set (JEF-1의 50군 단면적에 의한 고속 임계실험 해석)

  • Kim, Jung-Do;Gil, Choong-Sup;Kim, Young-Cheol
    • Nuclear Engineering and Technology
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    • v.25 no.3
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    • pp.457-469
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    • 1993
  • JEF-1-based 50-group cross section set for fast reactor calculations was generated using NJOY system. The set was then examined by analyzing measured integral quantities such as criticality and central reaction rate ratios for 27 fast critical assemblies. The calculated results using the new set were also compared with those of ENDF/B-IV or-V-based fast set. In general, the JEF-1-based set shows an improvement in predicting measured integral quantities in comparison with the previous set. With a few exceptions, JEF-1 results are comparable to those of ENDF/B-V.

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Prediction of $EC_{50}$ of Photobacterium phosphoreum for CAHs and Chlorophenol Derivatives Using QSAR (QSAR방법을 이용한 CAHs와 Chlorophenol 유도체에 대한 $EC_{50}$값 예측)

  • Lee, Hong-Joo;Yoo, Seung-O;Lee, Jeong-Gun;Kim, Byung-Yong;Chun, Uck-Han
    • Microbiology and Biotechnology Letters
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    • v.27 no.1
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    • pp.54-61
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    • 1999
  • Measurement of inhibition of bioluminescence in Photobacterium phosphoreum has been porposed as a sensitive and rapid procedure to monitor toxic substances. However, at first, $EC_{50}$ which shows degree of toxicity to each toxic substances must be calculated. QSAR (Quantitative Structure Activity Relationship) model can be used to estimate $EC_{50}$ to save time and endeavor. Moderately high correlation coefficients ($r^2{\geq}$ 0.97) were calculated from the linear correlation between $EC_{50}$ and molecular connectivity indices of CAHs (chlorinated aliphatic hydrocarbons)such as $^0X$, $^0X^V$, $^1X$, $^2X$ and $^3X^v_c$ and quadratic correlation between $EC_{50}$ and $^0X$, $^0X^V$, $^2X^V$, $^3X_c$, $^3X^V_c$ and P. It shows that the molecular connection indices in carbon structure is contributed to biological characters with linear relation and that in the other one with quadratic relation. The $EC_{50}$ of chlorophenol derivatives had quadratic relation with the value of octanol/water prtition coefficients ($r^2$=0.99) and linear and quadratic relation with the number of chlorine compound (($r^2{\geq}$0.94). This confirms the already known trend of increasing toxicity with increasing ability of a compound to diffuse through cell membrane and number of chlorine substitution.

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Luminous Characteristics of AC-PDP with Ridged Dielectric Layer in Various Xe Contents (능선형상의 유전체 구조를 갖는 AC-PDP의 Xe 함량 변화에 대한 발광특성)

  • 남문호;김정민;최시영
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.41 no.8
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    • pp.25-30
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    • 2004
  • In this paper, we suggest a new front panel structure with ridged front panel dielectric layer and hollow gap. The proposed structure can decrease a driving voltage and increase Xe content due to strong electric fields. In the experiment, we have used 6" test panel with 10 %, 15 %, 20 %, 30 % and 50 % Xe contents and 450 torr gas pressure. When comparing with a conventional structure in Xe 10 %, the proposed structure with same Xe content decreased a firing and sustain voltage by about 74 V and 79 V, also the luminance and luminous efficacy of proposed structure with 50 % Xe content were improved by about 33 % and 50.9 %..9 %.

Failure Mode of the Positive Plate on Charging Voltage in Gel Type Valve Regulated Lead Acid Batteries (충전전압에 따른 겔형 VRLA전지 양극판의 파손방식)

  • Oh Sanghyub;Kim Myungsoo;Lee Heung Lark
    • Journal of the Korean Electrochemical Society
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    • v.3 no.2
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    • pp.90-95
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    • 2000
  • Cycle life test was carried out to evaluate the failure modes of the gel type nth batteries at $C_5$ currents and $100\%$ DOD. When the batteries were charged at constant voltage of 2.40 V and 2.50 Vi respectively, cycle lift was over 1,000 cycles. The batteries lost 426.4 g and 391.2 g of electrolyte far each case after the weight measurement. The battery charged at 2.50 V was shown to have a better cyclic performance than charged at 2.40 V, and the amounts of electrolyte loss was proportional to charge factor. After cycle test, the micro-structure of positive active material was completely changed and the corrosion layer of positive grid was about $50{\mu}m$. Failure mode of the positive plate of the gel type battery was a shedding of the positive active material, and the cause of discharge capacity decrease was found to be a electrolyte loss.

Effect Analysis of Mix Designing Factors on Workability and Rheological Parameters of Self-Compacting Concrete (배합요인이 자기충전 콘크리트의 워커빌리티 및 레올로지 파라미터에 미치는 영향 분석)

  • Yoon, Seob;Han, Min-Cheol
    • Journal of the Korea Institute of Building Construction
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    • v.18 no.3
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    • pp.235-242
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    • 2018
  • The objective of the paper is to investigate the effect of mix designing factors on the workability and rheological parameters of self compacting concrete in order to facilitate the difficulties of quality control of high sensitivity of SCC. Mix proportions of SCC were prepared with various conditions of coarse, and fine aggregate, and unit water content, and the SCC mixtures were tested on workability, rheological properties to provide basic data for quantitative evaluation. Test results indicated that the yield stress of SCC decreased with increasing the coarse aggregate volume ratio, and increased with increasing the amount of VMA. However, unit water content, fine aggregate type, and air content didn't affect the yield stress value. The plastic viscosity according to the mixing factors showed a similar tendency to the yield stress. In addition, there was no correlation between yield stress and workability (flow, T50, V-lot). However, there was closely correlation among plastic viscosity and T50 and V-lot. Especially, T50 and V-lot time decreased with decreasing plastic viscosity.

Follow up study of pulmonary function after pneumonectomy (일측 폐절제술후 폐기능의 추적관)

  • Park, Jae-Gil;Kim, Se-Hwa;Lee, Hong-Gyun
    • Journal of Chest Surgery
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    • v.16 no.4
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    • pp.539-546
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    • 1983
  • Maximal expiratory flow-volume [MEFV] curves were studied in 22 patients who underwent pneumonectomy with various pulmonary lesions, such as lung cancer, bronchiectasis and tuberculosis etc, at the preoperative stage and 3 week, 4 month and 12 month after pneumonectomy for the analysis of the reduction and progressive improvement of postoperative ventilatory function. And the factors affecting them like as age difference and the site of pneumonectomy were also analyzed. From these curves peak flow rate [PF R], maximal expiratory flows at 25% and 50% of expired forced vital capacity [V25, V50] and forced vital capacity [FVC] were obtained. In addition, partial pressure of oxygen and carbon dioxide in arterial blood were measured. The results were as follows; 1. The mixed type, especially obstructive type of ventilatory impairment was observed at 3 week after operation. For 1 year of postpneumonectomy FVC was increased by 12.3% of predicted compared to 2.6% of predicted V50. 2. The improvement of FVC during 1 year of postpneumonectomy showed decreasing tendency with the increase of age but the changes of V25 and V50 were unremarkable. 3. The differences of immediate postoperative reduction and progressive improvement of ventilatory capacity after right and left pneumonectomy were analyzed. The reduction of V50, V25 and FVC at 3 week of postoperation were greater in patients with right pneumonectomy [20.9%, 18.2% and 26.2% of predicted] than in patients with left pneumonectomy 16.5%, 18.2% and 18.1%]. But there was no significant difference of these values at 12 month after pneumonectomy. 4. The partial pressure of oxygen in arterial blood [$PaO_2$] was decreased by 13.6 mmHg at 3 week after pneumonectomy compared to the preoperative stage but returned to the normal range within 4 month after pneumonectomy. However, TEX>$PaCO_2$ was within the normal range during 1 year of postoperation.

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Temperature-dependent characteristics of Current-Voltage for Double Gate MOSFET (동작 온도에 따른 Double Gate MOSFET의 전류-전압특성)

  • 김영동;고석웅;정학기
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2003.05a
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    • pp.693-695
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    • 2003
  • In this paper, we have investigated temperature-dependent characteristics of current-voltage for double gate MOSFET with main gate and side gate. DG MOSFET has the main gate length of 50nm and the side gate length of 70nm. We have investigated the temperature-dependent characteristics of current-voltage and drain voltage is changed from 0V to 5.0V at $V_{mg}$ =1.5V and $V_{sg}$ =3.0V. We have obtained a very good characteristics of current-voltage for 77K. We have simulated using ISE-TCAD tool for characteristics analysis of device.

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