• Genomics & Informatics
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• v.19 no.3
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• pp.33.1-33.10
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• 2021

Eucalyptus is one of the major plantation species with wide variety of industrial uses. Polymorphic and informative simple sequence repeats (SSRs) have broad range of applications in genetic analysis. In this study, two individuals of Eucalyptus tereticornis (ET217 and ET86), one individual each from E. camaldulensis (EC17) and E. grandis (EG9) were subjected to whole genome resequencing. Low coverage (10×) genome sequencing was used to find polymorphic SSRs between the individuals. Average number of SSR loci identified was 95,513 and the density of SSRs per Mb was from 157.39 in EG9 to 155.08 in EC17. Among all the SSRs detected, the most abundant repeat motifs were di-nucleotide (59.6%-62.5%), followed by tri- (23.7%-27.2%), tetra- (5.2%-5.6%), penta- (5.0%-5.3%), and hexa-nucleotide (2.7%-2.9%). The predominant SSR motif units were AG/CT and AAG/TTC. Computational genome analysis predicted the SSR length variations between the individuals and identified the gene functions of SSR containing sequences. Selected subset of polymorphic markers was validated in a full-sib family of eucalypts. Additionally, genome-wide characterization of single nucleotide polymorphisms, InDels and transcriptional regulators were carried out. These variations will find their utility in genome-wide association studies as well as understanding of molecular mechanisms involved in key economic traits. The genomic resources generated in this study would provide an impetus to integrate genomics in marker-trait associations and breeding of tropical eucalypts.

• The Journal of the Convergence on Culture Technology
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• v.10 no.1
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• pp.453-458
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• 2024

Due to global issues such as climate crisis and rising energy costs, there is an increasing focus on energy conservation and management. In the case of South Korea, approximately 53.5% of the total energy consumption comes from industrial complexes. In order to address this, we aimed to improve issues through the 'Shared Network Utility Plant' among companies using similar energy utilities to find energy-saving points. For effective energy conservation, various techniques are utilized, and stable data supply is crucial for the reliable operation of factories. Many anomaly detection and alert systems for checking the stability of data supply were dependent on Energy Management Systems (EMS), which had limitations. The construction of an EMS involves large-scale systems, making it difficult to implement in small factories with spatial and energy constraints. In this paper, we aim to overcome these challenges by constructing a data collection system and anomaly detection alert system on embedded devices that consume minimal space and power. We explore the possibilities of utilizing anomaly detection alert systems in typical institutions for data collection and study the construction process.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.5
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• pp.1-9
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• 2018

The purpose of this study was to identify differences of perceptions for gardens and parks between experts and the general public concerning several aspects including scope, scale, publicity, artistic and scientific nature, main materials, practicality and aesthetics, executive and management systems as well as legal understanding of garden and park. The properties of garden and park were derived through literature research, and the concept, similarity, and difference of gardens and the parks were recognized by the experts and the public viewpoint was clarified by questionnaire. As for the difference in the scope of the gardens and the parks, the expert group recognized it more widely than the general public. In general, the space recognized as a garden was the rooftop green space, and urban forests were recognized as a park. In addition, the general public recognized urban forests as gardens the same as they recognized parks, and the distinction was unclear. In the expert group, the perception that gardens were small and the parks were large was more prevalent. It was generally recognized that gardens were private spaces and the parks were public spaces. In the expert group, the gardens were more personal and the parks were more apparent to the public. In the general population, functional and scientific aspects rather than artistic creativity in both gardens and parks. In addition, both the general public and experts found that parks are more complex than gardens. The garden was centered on plant material, and the park was recognized as a center where the sculptural facilities were centered, or the plant material and the sculptural facilities were properly balanced. To the experts the view of the gardens was positive. Expert groups emphasized the aesthetics of the garden, and the parks were more practical, and the general population showed similar perceptions of utility and aesthetics when comparing gardens and parks. In addition, the utility of gardens in the general publics is more emphasized than the aesthetics of the park. Regarding the executive system the park was recognized as the public sector, and the difference was larger in the expert group. As for the management system, both experts and the general public perceive the management of the park or the garden to be carried out by the supporting organization, and it is necessary to discuss the diversification of the management subject. It is found that there is a certain difference in recognition with the mixture of concepts, and there is still a big difference in legal system and perception.

• Research in Plant Disease
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• v.9 no.4
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• pp.229-236
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• 2003

The 1080 epiphytic bacteria obtained from 370 samples of pome and stone fruits including apple, pear, peach, grape, apricot and Chinese quince were screened for antagonistic activity against postharvest pathogens, Penicillium expansum, Alternaria alternata and Botrytis cinerea. Among tested antagonistic bacteria, eight bacterial isolates inhibited mycelial growth of the postharvest pathogens and were identified as Bacillus amyloliquefaciens (three strains), B. megaterium, B. subtilis var. gladioli, B. licheniformis, B. pumilus and Serratia marcescens based on biochemical characteristics and utility of carbon and nitrogen compounds (Biolog system). Eight carbohydrates were evaluated for their effect on mycelial growth and germination of the postharvest pathogen, P. expansum to select nutrients for enhancing bio-control efficacy. The growth of four selected antagonists, B. amyloliquefaciens P43-2, B. amyloliquefaciens A71-2, B. licheniformis P94-1, and S. marcescens P76-9 were also tested. As a result, 1% glucose (w/v) strongly stimulated growth of the antagonists, suppressed mycelial growth of the postharvest pathogen, and had a little comparatively stimulatory effect on germination of the the postharvest pathogen. It was confirmed that the addition of 1% glucose (w/v) greatly enhanced biocontrol effect of B. amyloliquefaciens P43-2, B. licheniformis P94-1, and S. marcescens P76-9. Application of B. amyloliquefaciens P43-2, B. licheniformis P94-1, and S. marcescens P76-9 with the addition of 1% glucose (w/v) increased the control efficacy up to 48%, 46%, 14% compared with those of the antagonists without glucose, respectively. When the antagonists were applied to control postharvest disease caused by P. expansum in apple wounds, the population of B. amyloliquefaciens P43-2 and B. licheniformis P94-1 increased until 4 days after inoculation (DAI) of the antagonists and then decreased from 10 DAI. Meanwhile the population of S. marcescens P76-9 decreased at early stage (4 DAI), but increased from 7 DAI, and finally maintained constantly until 10 DAI in apple wounds.

• Journal of the Korean Recycled Construction Resources Institute
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• v.6 no.2
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• pp.112-118
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• 2011

The recycled asphalt concrete has gotten increasing attention due to the environmental issues. The volume of reclaimed asphalt has increased significantly for last few years because of city remodeling, pavement maintenance, utility excavation, and road widening. Considering the value of reclaimed asphalt, it is rather used for the recycled asphalt concrete than it is used for fill and cover up material instead of soil. This research will be a supplements incomplete issues from existing research results and suggests the quality control guideline for recycled asphalt concrete and upcoming laws. As the first step of research, the trial construction of RAP(Recycled Asphalt Pavement) performed in expressway construction sites. These trial construction sites have been checked every years. And another construction sites studied and selected for more deeper performance check of RAP. For this checks, we used automatic pavement survey equipment and computerized analysis tools. Also, DSR(Dynamic Shear Rheometer) was used for the fatigue life calculation of binder blends(RAP and virgin binder). As a consequence of this research, the application of recycled asphalt provides good enough quality for highway construction. The preceeding literatures reviewed shows that the asphalt rejuvenator are used in many countries but that type of chemical agent are not used in Korea. By using the data of trial construction and mix design in Chongwon-Sangju construction lane, the surface and base courses consisted with the 10% and 30% rap mix asphalt section maintains good performance for up to 7 years. Through the performance check and laboratory tests(DSR), the quality control and mixture's low temperature prevention are the important factor and chemical agent necessary for increasing the fatigue life of RAP binder.

• Journal of the Korean Institute of Gas
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• v.21 no.5
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• pp.95-103
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• 2017

A lot of hazardous materials have been used for product processing and utility plant. Many accidents including toxic release, fire and explosions occur in the ammonia related facility and plant. Various safety and environment management program including PSM, SMS, ORA etc. are being implemented for risk management and accident prevention in the production industry. Also much study and research have been carried about risk assessment of accident scenario in the academic and research area. In this paper, firstly risk level was assessed by using a typically used KORA program and LOPA PFD method for the selected ammonia unloading and storage facility. And then risk reduction measures for the risk assessed facility were studied in 3 aspects and some measures were proposed. Those Risk Reduction measures are including a leak detection and emergency isolation, water spray, dilution tank, dike and trench, scattering protection in hardware impovement aspect, and a applicable risk criteria, conditional modifier for existing LOPA PFD, alternative supporting modeling program in risk estimation methodology aspect, and last RBPS(Risk Based Process Safety) program, re-doing of process hazard analysis, management system compliance audit in managerial activity aspect.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.8
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• pp.312-321
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• 2018

The Korean DR market proposes suppression of peak demand under reliability crisis caused a natural disaster or unexpected power plant accidents as well as saving power plant construction costs and expanding amount of reserve as utility's perspective. End-user is notified a DR event signal DR execution before one hour, and executes DR based on requested amount of load reduction. This paper proposes a DR energy management algorithm that can be scheduled the optimal operations of chiller system and ESS in the next day considering the TOU tariff and DR scheme. In this DR algorithm is divided into two scheduling's; day-ahead operation scheduling with temperature forecasting error and operation rescheduling on DR operation. In day-ahead operation scheduling, the operations of DR resources are scheduled based on the finite number of ambient temperature scenarios, which have been generated based on the historical ambient temperature data. As well as, the uncertainties in DR event including requested amount of load reduction and specified DR duration are also considered as scenarios. Also, operation rescheduling on DR operation day is proposed to ensure thermal comfort and the benefit of a COB owner. The proposed method minimizes the expected energy cost by a mixed integer linear programming (MILP).

• Korean Journal of Plant Taxonomy
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• v.43 no.4
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• pp.245-251
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• 2013

Paeonia lactiflora is a valuable natural resource for horticulture and traditional Chinese medicine. To propose conservation strategy and future utility of the wild Paeonia lactiflora populations recently found around the Gyeongju National Park, genetic diversity analysis using microsatellite markers were performed. Three populations in and near the Gyeongju N.P. and one population from Jilin, China were analyzed for five microsatellite markers, producing 61 alleles with mean observed heterozygosity($H_o$) of 0.452. $F_{ST}$ value (0.11642) suggested moderate level of genetic differentiation among the populations, and hierarchical AMOVA suggested most of the genetic variation resides within/among the individuals rather than among-population. While AMOVA with $F_{ST}$ suggested lack of genetic differentiation between the regional (Korean vs. Chinese) populations, AMOVA with $R_{ST}$, which incorporates the allele sizes, suggested considerable differentiation between them, but without significant statistical support. STRUCTURE analysis also suggested segregation of regional populations with presence of gene flow among the three Gyeongju N.P. populations. Considering small population size and scarcity of mature individuals, further protection and long-term monitoring are needed.

• Journal of Life Science
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• v.25 no.4
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• pp.441-449
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• 2015

Endlicheria anomala, a neotropical plant, is found in northern South America and the Amazon region. It is traditionally used to remove poisons and cure gangrene. According to recent data, this plant has diverse biological properties such as anti-oxidative, anti-inflammatory and anti-melanogenic properties. However, the anti-cancer effect of E. anomala and its molecular mechanisms remain unclear. In this study, we examined the anti-cancer effect and the active mechanism of methanol extract of E. anomala (MEEA) in human lung adenocarcinoma cells (A549) and human liver cancer cells (HepG2). Our data revealed that MEEA showed cytotoxic activity in a dose-dependent manner and induced apoptosis both in A549 and HepG2 cells. We verified evidences of apoptosis via formation of chromatin condensation, apoptotic body and accumulation of cells in the subG1 phase. Following observed apoptosis-related phenomena, we found that the induction of apoptosis by MEEA was associated with the increase of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 (WAF1/CIP1) expression. Furthermore, MEEA-induced apoptosis was characterized with proteolytic activation of caspase-3, degradation of poly ADP ribose polymerase (PARP), and up-regulation of pro-apoptotic Bax expression. Taken together, these findings indicate that MEEA may have potential cancer therapeutic utility in A549 and HepG2 cells.

• Journal of Energy Engineering
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• v.25 no.2
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• pp.94-100
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• 2016

ESS(Energy Storage System) is an important source that keeps power supply stable and utilizes electricity efficiently. For example, ESS contributes to resolve power supply imbalance, stabilize new renewable energy output and regulate frequency. ESS is predicted to be expanded to 55.9GWh of installed capacity by 2023, which is 30 times more than that of 2014. To raise competitiveness of domestic ESS industry in this increasing world market, we have disseminated load-shift ESS for continuous power supply imbalance with FR ESS, and also necessity to secure domestic track record is required. However in case of FR ESS, utility of installing thermal power plant is generally generated within 5% range of rated capacity, so that scalability of domestic market is low without dramatic increase of thermal power plant. Necessity of load-shift ESS dissemination is also decreasing effected by surplus backup power securement policy, raising demand for new dissemination model. New dissemination model is promising for $CO_2$ reduction effect in spite of intermittent output. By stabilizing new renewable energy output in connection with new renewable energy, and regulating system input timing of new renewable energy generation rate, it is prospected model for 'post-2020' regime and energy industry. This research presents a policy alternatives of REC multiplier calculation method to induce investment after outlining PV-connected ESS charge/discharge mode to reduce GHG emission, This alternative is projected to utilize GHG emission reduction methodology for 'Post-2020' regime, big issue of new energy policy.