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Molecular Breeding of Genes, Pathways and Genomes by DNA Shuffing

  • Stemmer, Willem P.C.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.3
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    • pp.121-129
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    • 2002
  • Existing methods for optimization of sequences by random mutagenesis generate libraries with a small number of mostly deleterious mutations, resulting in libraries containing a large fraction of non-functional clones that explore only a small part of sequence space. Large numbers of clones need to be screened to find the rare mutants with improvements. Library display formats are useful to screen very large libraries but impose screening limitations that limit the value of this approach for most commercial applications. By contrast, in both classical breeding and in DNA shuffling, natural diversity is permutated by homologous recombination, generating libraries of very high quality, from which improved clones can be identified with a small number of complex screens. Given that this small number of screens can be performed under the conditions of actual use of the product, commercially relevant improvements can be reliably obtained.

Generalized One-Level Rotation Designs with Finite Rotation Groups Part I:Generatio of Designs

  • Park, You-Sung;Kim, Kee-Whan
    • Journal of the Korean Statistical Society
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    • v.29 no.1
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    • pp.29-44
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    • 2000
  • In this paper, we consider one-level rotation designs with finite rotation groups such that the design satisfies two basic requirements: all rotation groups are included in any given survey period, and overlapping rates depend only on the time lag. First we present the necessary number of rotation groups and a rule for the length of time the sample units are to be in or out of the sample to satisfy the requirements. Second, an algorithm is presented to put rotation groups to proper positions in a panel in order to include all finite rotation groups for any survey period. Third, we define an one-level rotation pattern which is invariant in the survey period and has useful properties in practical sense.

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A study on The Assignment of Jangbu(臟腑) to Chon.Gwan.Cheok(寸.關.尺) in "Maekyojeongmiron(脈要精微論)" ("소문(素問).맥요정미론(脈要精微論)"의 촌관척(寸關尺) 장부배속(臟腑配屬)에 대한 고찰)

  • Yang, Kwang-Yeol
    • Journal of Korean Medical classics
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    • v.22 no.1
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    • pp.27-34
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    • 2009
  • In "Maekyojeongmiron(脈要精微論)", Jangbu(臟腑) is assigned to Chon.Gwan.Cheok(寸關尺) in according to the position. Kidney[腎] is assigned to the backward area of Cheok. Abdominal cavity[腹中] is assigned to the middle area of Cheok. Hypochondrium[季脇] is assigned to the forward area of Cheok. Liver[肝] is assigned to the backward area of left Gwan. Diaphragm[鬲] is assigned to the forward area of left Gwan. Stomach[胃] is assigned to the backward area of right Gwan. Spleen[脾] is assigned to the forward area of right Gwan. Heart[心] is assigned to the backward area of left Chon. Central part of the chest[膻中] is assigned to the forward area of left Chon. Lung[肺] is assigned to the backward area of right Chon. Thoracic cavity[胸中] is assigned to the forward area of right Chon. This method of assignment is simple and useful in clinic.

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Back Ground Noise of Borehole Seismic Data at Hyodongri (효동리 시추공 관측소의 배경잡음 특성)

  • 신진수
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2000.10a
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    • pp.41-48
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    • 2000
  • We have installed the borehole seismic recording system at Hyodongri in eastern part of Kyungsan Basin, which has the advantages of reduction in noise by human activities and distorting effects of near-surface rocks. Here we describe briefly the borehole seismic station and recording system. And we analyse the characteistics of back ground the station obtained from borehole sensors. The back ground noise level in time domain is about 50~100$\mu$cm/sec. The average curve of noise spectrum is lower than NHNM(New High Noise Model)of GSN(Global Seismic Network)operated by USGS. The results could be useful prior information for study on earthquake records observed at Hydongri station.

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PROCESS ANALYSIS OF AUTOMOTIVE PARTS USING GRAPHICAL MODELLING

  • IRIKURA Norio;KUZUYA Kazuyoshi;NISHINA Ken
    • Proceedings of the Korean Society for Quality Management Conference
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    • 1998.11a
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    • pp.295-300
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    • 1998
  • Recently graphical modelling is being studied as a useful process analysis tool for exploratory causal analysis. Graphical modelling is a presentation method that uses graphs to describe statistical models of the structures of multivariate data. This paper describes an application of this graphical modeling with two cases from the automotive parts industry. One case is the unbalance problem of the pulley, an automotive generator part. There is multivariate data of the product from each of the processes which are connected in the series. By means of exploratory causal analysis between the variables using graphical modeling, the key processes which causes the variation of the final characteristics and their mechanism of the causal relationship have become clear. Another case is, also, the unbalanced problem of automotive starter parts which consists of many parts and is manufactured by complex machinery and assembling process. By means of the similar technique, the key processes are obtained easily and the results are reasonable from technical knowledge.

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한반도 기후 변화에 따른 수해 및 빗물 저류터널(Flood Drainage Tunnel) 건설의 세계 동향 검토 연구

  • Choe, Jae-Hwa;Ji, Wang-Ryul
    • Magazine of korean Tunnelling and Underground Space Association
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    • v.14 no.2
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    • pp.31-37
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    • 2012
  • In the circumstances being continuous the unusual weather in the world, the city of Seoul has been devastating flood damage in July 2011, because of the heavy rainfalls. Along with expensive repairs to property, thousands of flood victims occurred; it is difficult to estimate the direct and indirect economic damages in city. Recently, as a part of the flood protecting measures, there are being discussed about the deep underground flood drainage tunnel, underground regulating reservoirs, permeable pavement, infiltration facility, river improvements, diversion channel, sewer pipe and ditch improvement and so on. Therefore, it is useful to make the plan of flood protecting measures more and more cost-effective and rational methods by considering the similar flood measures and constructions in the mega cities like Seoul.

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Occupational Safety and Health Activities Conducted across Countries in Asia

  • Park, Jung-Keun;Khai, Ton T.
    • Safety and Health at Work
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    • v.6 no.2
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    • pp.143-145
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    • 2015
  • Three occupational safety and health (OSH) activities, one international and two national workshops, were documented as part of OSH activities conducted under the International Labor Organization/Korea Partnership Program in the year 2011-2012. This study aimed to provide information on what the three OSH activities were implemented and how they contributed to the improvement of OSH in Asian countries. The international workshop was useful for the participants to understand a variety of information on OSH as well as participatory action-oriented training (PAOT) approaches at the regional and global levels. The two national workshops were practical for participants to strengthen their knowledge and skills on the PAOT at the enterprise and national levels. The study shows that the three OSH activities contributed to the understanding of the participants on OSH and PAOT, and that the activities promoted the improvement of OSH across countries in Asia.

Effective Learning Tasks and Activities to Improve EFL Listening Comprehension

  • Im, Byung-Bin
    • English Language & Literature Teaching
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    • no.6
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    • pp.1-24
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    • 2000
  • Listening comprehension is an integrative and creative process of interaction through which listeners receive speakers' production of linguistic or non-linguistic knowledge. Compared with reading comprehension, it may arouse difficulties and thus impose more burdens on foreign learners. The Audio-Lingual Method focused primarily on speaking. Mimicry, repetition, rote memory, and transformation drills actually interfered with listening comprehension. So learners lost interest and were not highly motivated. Improving listening comprehension requires continual attentiveness and interest. Listening skill can be extended systematically only when students are frequently exposed to a wide range of listening materials with an affective, cultural, social, and psycholinguistic approach. Therefore, teachers should help students learn how to comprehend intactly the overall meaning of intended messages. The literature on teaching listening skill suggests various useful activities: TPR, dictation, role playing, singing, picture recognition, completion, prediction, seeking specific information, summarizing, labeling, humor, jokes, cartoons, media, and so on. Practical classroom teaching necessitates a systematic procedure in which students should take part in meaningful tasks/activities. In addition to this, learners must practice listening comprehension trough a self-study process.

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EIGHT-DIMENSIONAL EINSTEIN'S CONNECTION FOR THE FIRST CLASS I. THE RECURRENCE RELATIONS IN 8-g-UFT

  • HWANG, IN HO;CHUNG, KYUNG TAE;HAN, SOO KYUNG
    • Honam Mathematical Journal
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    • v.28 no.4
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    • pp.605-639
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    • 2006
  • Lower dimensional cases of Einstein's connection were already investigated by many authors for n = 2,3,4,5,6,7. This paper is the first part of the following series of two papers, in which we obtain a surveyable tensorial representation of 8-dimensional Einstein's connection in terms of the unified field tensor, with main emphasis on the derivation of powerful and useful recurrence relations which hold in 8-dimensional Einstein's unified field theory(i.e., 8-g-UFT): I. The recurrence relations in 8-g-UFT II. The Einstein's connection in 8-g-UFT All considerations in these papers are restricted to the first class only of the generalized 8-dimensional Riemannian manifold $X_8$.

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REMARKS ON FINITE ELEMENT METHODS FOR CORNER SINGULARITIES USING SIF

  • Kim, Seokchan;Kong, Soo Ryun
    • Honam Mathematical Journal
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    • v.38 no.3
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    • pp.661-674
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    • 2016
  • In [15] they introduced a new finite element method for accurate numerical solutions of Poisson equations with corner singularities, which is useful for the problem with known stress intensity factor. They consider the Poisson equations with homogeneous Dirichlet boundary condition, compute the finite element solution using standard FEM and use the extraction formula to compute the stress intensity factor, then they pose a PDE with a regular solution by imposing the nonhomogeneous boundary condition using the computed stress intensity factor, which converges with optimal speed. From the solution we could get accurate solution just by adding the singular part. This approach works for the case when we have the accurate stress intensity factor. In this paper we consider Poisson equations with mixed boundary conditions and show the method depends the accrucy of the stress intensity factor by considering two algorithms.