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Characteristics of Microwave-assisted Extraction for Catechins from Grape Seed (포도씨 카테킨류의 마이크로웨이브 추출특성)

  • Lee, Eun-Jin;Choi, Sang-Won;Kim, Hyun-Ku;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.40 no.5
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    • pp.510-515
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    • 2008
  • Microwave energy was applied to the extraction of functional catechins from grape seed. The solvent, absolute ethanol, reached the boiling point when exposed for less than 3 min microwave treatment at 100 W. The effects of independent variables in microwave-assisted extraction (MAE), including microwave power (0-160W, $X_1$), ethanol concentration (0-100%, $X_2$) and extraction time (1-5 min, $X_3$), were investigated on each response variable ($Y_n$), and the contents of catechin and its derivatives were determined via response surface methodology, thereby allowing us to predict their optimal extraction conditions. The predicted maximal values of (+)-catechin, procyanidin $B_2$, (-)-epicatechin, and (-)-epicatechin gallate were 137.99, 72.78, 222.38, and 9.59 mg%, respectively, under different MAE conditions. The predicted extraction conditions for maximum catechin responses were as follows: 104.10 W of microwave power, 45.35% of EtOH, and 4.89 min of extraction time for (+)-catechin (137.99 mg%), 133.16 W, 46.16% and 4.49 min for procyanidin $B_2$ (72.78 mg%), 136.00 W, 41.37% and 4.39 min for (-)-epicatechin (222.38 mg%), 143.20 W, 37.51% and 1.88 min for (-)-epicatechin gallate (9.59 mg%), respectively. The contents of (+)-catechin, procyanidin 1B2 and (-)-epicatechin in MAE were similarly influenced by three independent variables, whereas (-)-epicatechin gallate was influenced less profoundly by ethanol concentration and extraction time.

Comparison of Molecular Linkage Maps and QTLs for Morphological Traits in Two Reciprocal Backcross Populations of Rice

  • Qiao, Yongli;Jiang, Wenzhu;Rahman, Md Lutfor;Chu, Sang-Ho;Piao, Rihua;Han, Longzhi;Koh, Hee-Jong
    • Molecules and Cells
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    • v.25 no.3
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    • pp.417-427
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    • 2008
  • Comparison of maps and QTLs between populations may provide us with a better understanding of molecular maps and the inheritance of traits. We developed and used two reciprocal $BC_1F_1$ populations, IP/DS//IP and IP/DS//DS, for QTL analysis. DS (Dasanbyeo) is a Korean tongil-type cultivar (derived from an indica x japonica cross and similar to indica in its genetic make-up) and IP (Ilpumbyeo) is a Korean japonica cultivar. We constructed two molecular linkage maps corresponding to each backcross population using 196 markers for each map. The length of each chromosome was longer in the IP/DS//IP population than in the IP/DS//DS population, indicating that more recombinants were produced in the IP/DS//IP population. Distorted segregation was observed for 44 and 19 marker loci for the IP/DS//IP and IP/DS//DS populations, respectively; these were mostly skewed in favor of the indica alleles. A total of 36 main effect QTLs (M-QTLs) and 15 digenic epistatic interactions (E-QTLs) were detected for the seven traits investigated. The phenotypic variation explained (PVE) by M-QTLs ranged from 3.4% to 88.2%. Total PVE of the M-QTLs for each trait was significantly higher than that of the E-QTLs. The total number of M-QTLs identified in the IP/DS//IP population was higher than in the IP/DS//DS population. However, the total PVE by the M-QTLs and E-QTLs together for each trait was similar in the two populations, suggesting that the two $BC_1F_1$ populations are equally useful for QTL analysis. Maps and QTLs in the two populations were compared. Eleven new QTLs were identified for SN, SF, GL, and GW in this study, and they will be valuable in marker-assisted selection, particularly for improving grain traits in tongil-type varieties.

Present Status of Fermented Milk Products in Japan

  • Hosono, Akiyoshi
    • 한국유가공학회:학술대회논문집
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    • 2004.06a
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    • pp.21-39
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    • 2004
  • Fermented milks have been recognized as healthy foods since ancient times, but those using intestinal bacteria such as Bifidobacterium and lactic acid bacteria (LAB) are even more valuable from the standpoint of view of maintaining health. They have also now come to be recognized as important in the field of preventive medicine. Although advances in the medical sciences in the last 50 years have significantly increased the human lift span, an unfortunate fact is that many of us are now living long enough to experience chronic disorders such as coronary heart disease, hypertension, osteoporosis, diabetes and cancer. In recent years there has been renewed interest in health promotion and disease prevention by incorporating probiotic bacteria into foods to counteract harmful bacteria in the intestinal tract. Therefore, there are now a wide variety of commercial products containing prospective probiotics that claim health-promoting effects, such reductions in large botvel carcinogens and mutagens, antitumor properties, cholesterol-lowering effects, increased lactose digestion, relief from con-stipation, stimulation of immunocomponent cells and enhancement of phagocytosis. Two well-known representative probiotic is LAB and Bifidobaclerium. Traditional probiotic dairy strains of LAB which have been designated as GRAS (Generally Recognized As Safe) bacteria have a long history of safe use and most strains are considered comestible microorganisms with no pathogenic potential. Accordingly, there is considerable interest in extending the range of foods containing probiotic organisms from dairy foods to infant formulas, baby foods, and pharmaceuticals. In addition, the ingestion of probiotics, prebiotics, and symbiotic as wll as combinations of pro- and prebiotics has recently aroused renewed interest as enhancing the beneficial relationship between the host and intestinal microflora in both healthy and diseased indivisuals. Non-communicable chronic diseases such as cancer, cerebral hemorrhage, is chemic heart disease, and diabetes mellitus has recently been recognized as adult diseases in Japan as well as other countries. and are considered to be inevitably associated with aging. These diseases occur as a result of individual life styles. The Japanes Government. Ministry of Health, Labor and Welfare has proposed substituting the term 'adult diseases' with 'lifestyle-related diseases'. It has emphasized the importance of prevention rather than treatment. since the well-known increase in the elderly population in Japan is predicted to result in a variety of socioeconomic problems. n this lecture on the Present status of fermented milk products in Japan, I will report a strategy for the development of fermented milk products in Japan from the standpoint of view of research in Japan on LAB and Bifidobacteria. They could play an important role in preserving human health by controlling intestinal microflora capable of producing toxic effects on the host.

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Fractionation and Electrophoretic Patterns of Rice Proteins (쌀단백질의 분획 및 전기영동)

  • Kim, Su-Il;Jo, Do-Hyun
    • Applied Biological Chemistry
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    • v.26 no.1
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    • pp.65-72
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    • 1983
  • The composition of four rice protein groups is greatly affected by the extraction conditions. The extraction amounts of albumins and glubulines primarily depended on the temperature rather than the method of extraction. The total amount of glutelins, the major components of rice storage proteins, could be extracted by a successive extraction processes, extraction with 0.5% SDS-0.1M borate buffer(pH 8.3) followed by extraction with 0.5% SDS-0.6% ${\beta}-mercaptoethanol-0.1M$ borate buffer(pH 8.3). The extracted amounts of glutelin with these solvents were 54.1 and 45% respectively. The further purification of SDS soluble glutelins was achieved by Sephadex G-150 gel column chromatography. The molecular weight of the components in four protein groups has been estimated by SDS-polyacrylamide gel electrophoresis with or without ${\beta}-mercaptoethanol.$ The comparison of albumins and globulins by starch gel electrophoresis at pH 3.1 permitted us to identify seven rice varieties. However, at pH 8.95, the specific bands for Japonica type rice varieties were observed.

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Old Age Early Retirement and Careering Ending Patterns : Centering around the US Older Men (노년기 조기퇴직과 경력마감 형태 : 남성노인을 중심으로)

  • Lee, Hyun-Kee
    • Korean Journal of Social Welfare
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    • v.52
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    • pp.33-61
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    • 2003
  • This study aims to identify early retirement and the subsequent career ending patterns and explain the phenomena in terms of social security policies and labor market perspectives. Although many older workers retire early, there are very few studies to examine how the retirees end their careers after quitting the long-time jobs. The paper investigates individual work experiences 55 through 61, identifies the career ending patterns, constitutes the micro and macro analytical models to estimate the impacts of labor market and work-related variables. The findings show that many older workers have diverse career ending patterns after long-time career jobs, that is, still working at age-55 job, exiting and reentering, sporadic work, retired permanently from age-55 job, a repeat of labor force entry. Also the estimation results indicate that pension, social security, labor market condition, work structure variables better explain the career ending behaviors than social psychological factors of health, education, attitudes toward retirement and job, ethnicity. Finally, this paper discusses theoretical importances of labor market perspectives and policy implications at the end of thesis.

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Schottky barrier overlapping in short channel SB-MOSFETs (Short Channel SB-FETs의 Schottky 장벽 Overlapping)

  • Choi, Chang-Yong;Cho, Won-Ju;Chung, Hong-Bay;Koo, Sang-Mo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.133-133
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    • 2008
  • Recently, as the down-scailing of field-effect transistor devices continues, Schottky-barrier field-effect transistors (SB-FETs) have attracted much attention as an alternative to conventional MOSFETs. SB-FETs have advantages over conventional devices, such as low parasitic source/drain resistance due to their metallic characteristics, low temperature processing for source/drain formation and physical scalability to the sub-10nm regime. The good scalability of SB-FETs is due to their metallic characteristics of source/drain, which leads to the low resistance and the atomically abrupt junctions at metal (silicide)-silicon interface. Nevertheless, some reports show that SB-FETs suffer from short channel effect (SCE) that would cause severe problems in the sub 20nm regime.[Ouyang et al. IEEE Trans. Electron Devices 53, 8, 1732 (2007)] Because source/drain barriers induce a depletion region, it is possible that the barriers are overlapped in short channel SB-FETs. In order to analyze the SCE of SB-FETs, we carried out systematic studies on the Schottky barrier overlapping in short channel SB-FETs using a SILVACO ATLAS numerical simulator. We have investigated the variation of surface channel band profiles depending on the doping, barrier height and the effective channel length using 2D simulation. Because the source/drain depletion regions start to be overlapped each other in the condition of the $L_{ch}$~80nm with $N_D{\sim}1\times10^{18}cm^{-3}$ and $\phi_{Bn}$ $\approx$ 0.6eV, the band profile varies as the decrease of effective channel length $L_{ch}$. With the $L_{ch}$~80nm as a starting point, the built-in potential of source/drain schottky contacts gradually decreases as the decrease of $L_{ch}$, then the conduction and valence band edges are consequently flattened at $L_{ch}$~5nm. These results may allow us to understand the performance related interdependent parameters in nanoscale SB-FETs such as channel length, the barrier height and channel doping.

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The Soil and Water Pollution caused by the Weathering of Pyrophyllite Deposits: Upstream Part of Hoidong Water Reservoir in Pusan (납석광산에서 발생하는 토양 및 수질오염 실태 : 부산광역시 회동수원지 상류 지역)

  • 박맹언;김근수
    • Journal of Environmental Science International
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    • v.7 no.2
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    • pp.149-156
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    • 1998
  • Enoronmental problems caused by certain geologic conditions Include pollution of soil by heavy metal, acidization of souls , acid mine drainage, Pound-water pollution, and natural radioactivity, as well as zoo-logical hazards such as landslide and subsidence. The acrid mine drainage contains large amount of heavy metals nO, therefore. cause serious pollution onto the nearby drainage systems and soils. In spite of this prospective environmental danger, few studies have been done on the acid mine drainage derived from non-metallic ore deposits such as pyrophyllitefNapseok) deposits. The sudo-bearing pyrophyllite ores, alteration zones, and mine talllngs of pyrophylllte deposits produce acrid mine drainage by the okidation of weathering. Compared to the fresh host rocks, the ores and altered rocks of pyrophyllite deposits produce acidic solution which contain higher amount of heavy metals because of OeP lower buffering capacity to acrid solution. The pus of urine water and nearby stream water of pyrophyllite deposits are 2.1~3.7, which are strong- ly acidic and much lower than that (6.2~7.2) of upstream water and than that (6.8~7.6) of the stream water derived from the non-mineralized area. This study reveals that this acrid mine drainage can affect the downstream area which is 8km far from the pyrophyllite deposits, even though the drain Is diluted with abundant non-contaminated river water This suggmists that not only acid mine drainage but also the sulfide-bearing sediments originated from the pyrophyllite deposits move downstream and form acidic water through continuous oxidation reaction. The heavy metals such as Pb, Zn, Cu, Cd, Nl, Mn and Fe are enriched In the mine water of low pH, and their contents decrease as the pH of mine water Increases because of the Influx of fresh stream wainer. SoUs of the Pyrophyulte deposits are characterized by high contents of heavy metals. The stream sediments containing the yellowish brown precipitates formed by neutralization of acid mine drainage occur in all parts of the stream derived from the pyrophyllite deposits, and the sediments also contain high amounts of heavy metals. In summary, the acid mine drainage of the pyrophyllite deposits is located in the upstream part of Hoidong water reservoir in Pusan contains large amounts of heavy metals and flows into the Holdong water reservoir without any purification process. To protect the water of Holdong reservoir, the acid mine drainage should be treated with a proper purification process.

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Reflecting on the History and Future of Republic of Korea Navy (대한민국 해군창설 : 회고와 당부)

  • Hahm, Myung-Soo
    • Strategy21
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    • s.37
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    • pp.5-31
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    • 2015
  • The Republic of Korean Navy (ROKN) started from scratch. However, ROKN demonstrated its Blue Navy capabilities successfully to the entire world by conducting "Operation Early Dawn" at the Aden Bay, Yemen in Jan 2011. On the event of the 70th anniversary of the ROKN, I would like to retrospect past gleaning from voyages and challenges we had in the past. At the very inception of the ROKN, Korean government as well as senior military leaders recognized that it had no time to spare to clean up military those were insinuated deeply by communist agents. It was the top priority of the government. The Mongumpo Operation which was not well known, conducted by ROKN was one of the clean-up drive. The Korean War sometimes called as "a fire from land put-off at the sea". The world famous "Incheon Landing" which reversed war situation from the Nakdong Perimeter also done by Sea Power. ROKN conducted various maritime operations including not only Incheon Landing, but amphibious operation at Hungnam, mine sweeping, sea convoy, Wonsan Withdrawal. On the same day of the Korean War started, 25th June 1950, unless the victory of the ROKNS Baekdusan (PC 701) at the Korean Strait, the waning lamp light of Korea could not be rekindled by the participation of the U.N. The ROKN rescued the 17th regiment of Korean Army from the isolation at the Ongjin Peninsular and transported gold and silver bars stored at the Bank of Korea to the Navy supply deposit in Jinhae safely. ROKN special intelligence unit conducted critical HUMINT which led Incheon Landing success. One of important mission ROKN conducted successfully was not only transporting war fighting materials but also U.S. provided grains to starving Koreans. ROKN participated Vietnam campaign from 1960s and conducted numerous maritime transportation operations supplying materials to Vietnam military forces along the long coastal lines. Experienced Naval Officers and enlisted men who discharged and acquired as merchant marine certificate supported most of the U.S. sea lift operations throughout the Vietnam campaign. ROK-US Combined Forces which had been honed and improved its war fighting capabilities through the Korean War and out of Vietnam jungle playing key deterrent against threat from north Korea. However, those threat level will be completely different when north Korea finish its nuclear weapon ambition. In order to stand firm against north Korean nuclear threat, I would like to expect strong political leadership supporting nuclear submarine for ROKN.

A Java Distributed Batch-processing System using Network of Workstation (워크스테이션 네트워크를 이용한 자바 분산 배치 처리 시스템)

  • Jeon, Jin-Su;Kim, Jeong-Seon
    • Journal of KIISE:Computing Practices and Letters
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    • v.5 no.5
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    • pp.583-594
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    • 1999
  • With the advance of VLSI and network technologies, it has now become a common practice to deploy a various forms of distributed computing environments. A study shows that a lot of network-aware computers are in an idle state for considerable amount of time depending on the types of users and time frames of the day. If we can take the full advantage of those idle computers, we can obtain the enormous combined processing power without further costly investment. In this paper, we present a distributed batch-processing system, called the Java Distributed Batch-processing System (JDBS), which allows us to execute CPU-intensive, independent jobs across a pool of idle workstations on top of extant distributed computing environments. Since JDBS is implemented using a Java programming language, it not only extends the scope of machine types that can be joined to the pool, but makes it a lot easier to build an entire system. Besides, JDBS is scalable and fault-tolerant due to its multi-cluster organization and intelligent strategies. A graphical user interface is also provided to facilitate the registration and unregistration, job submission, and job monitoring.

Biomass Estimation of Phyllostachys pubescens Stands in KFRI, Southern Forest Research Center (맹종죽 시험림의 현존량 추정에 관한 연구)

  • Lee, Kwang Soo;Jung, Su Young;Son, Yeong Mo;Lee, Kyeong Hak;Bae, Eun Ji;Yun, Seok Lak
    • Journal of Korean Society of Forest Science
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    • v.101 no.1
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    • pp.138-147
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    • 2012
  • For biomass estimation of Phyllostachys pubescens stands by optimal survey method we established 9 bamboo sample plots in the research forests of KFRI (Korea Forest Research Institute). The dry weight of culm segment determined by relative heights of total bamboo height show us two groups of 1st to 5th culm segment (up to 0~55% culm of tree height from the bottom area) and 6th to 8th culm segment (55~100%) by the results of cluster analysis for dry weight ratio. This results show that upper and lower part of 55~70% reference height from the bottom area against total culm height can be used in obtaining 1 kg of a sample bamboo, respectively, rather than 2.0 m stem segments of other forest tree species. In above-ground biomass estimation by $W=aD+bD^2$ having the highest coefficient of determination in this study, above ground stand biomass was 57.77 ton/ha (culm 40.47 ton/ha, branch 9.29 ton/ha, and leaf 8.01 ton/ha) of which 70% was contributed by culm component followed by branch (16%) and leaf (14%). Below-ground biomass was 53.35 ton/ha in total.