• Title/Summary/Keyword: Unstable Region

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Studies on Acetanilide p-Hydroxylase in Streptomyces spp. (Streptomyces 속 중의 Acetanilide p-Hydroxylase에 관한 연구)

  • Kim, Jung-Ae;Lee, Sang-Sup
    • YAKHAK HOEJI
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    • v.32 no.5
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    • pp.295-303
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    • 1988
  • For microbial production of acetaminophen, a popular analgesic-antipyretic from aniline, we screened various fungi and bacteria. And we succeeded to some extents in acetaminophen production by successful protoplast fusion between S. lividans and S. globisporus and also between S. rimosus and S. aureofaciens. However, more fertile results might be brought via performing the cloning of acetanilide p-hydroxylation genes of Streptomyces in yeast. This study was initiated to determine whether the acetanilide p-hydroxylase of Streptomyces is cytochrome P-450 species or non-heme iron protein species. The p-hydroxylationactivity on acetanilide in S. aureofaciens ATCC 10762 was found to be unstable on exposing to the air. However, 100,000xg supernatant of the cell free extracts which were prepared in $N_2$ atmosphere showed the p-hydroxylation activity. Characteristic absorption peak of cytochrome P-450 after reduction with dithionite and addition of CO was not observed in the region of 450nm. Moreover, metyrapone and 2, 6-dichloroindophenol did not affect this enzyme activity, but sodium azide, sodium cyanide, cupric sulfate, cadmium chloride, ${\alpha}$, ${\alpha}'-dipyridyl$, and o-phenanthroline reduced p-hydroxylase activity considerably. S. fradiae NRRL 2702 was shown to have strong p-hydroxylation activity in intact cells. This activity disappeared in its cell free extracts. In its 100,000xg supernatant, however, characteristic absorption peak of cytochrome P-450 after reduction with dithionite and addition of CO was observed at 446nm. Thus, the results herein presented suggest that acetanilide p-hydroxylase of Streptomyces aureofaciens is not related to cytochrome P-450 and may include non-heme iron protein for its activity. However, it is not clear whether acetanilide p-hydroxylase in S. fradiae belongs to the same category of S. aureofaciens.

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A Case Study of Tsukuba Tornado in Japan on 6 May 2012

  • Choo, Seonhee;Min, Ki-Hong;Kim, Kyung-Eak;Lee, Gyuwon
    • Journal of the Korean earth science society
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    • v.39 no.5
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    • pp.403-418
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    • 2018
  • This study conducted synoptic and mesoscale analyses to understand the cause of Japan Tsukuba tornado development, which occurred at 0340 UTC 6 May 2012. Prior to the tornado occurrence, there was a circular jet stream over Japan, and the surface was moist due to overnight precipitation. The circular jet stream brought cold and dry air to the upper-level atmosphere which let strong solar radiation heat the ground with clearing of sky cover. A tornadic supercell developed in the area of potentially unstable atmosphere. Sounding data at Tateno showed a capping inversion at 900 hPa at 0000 UTC 6 May. Strong insolation in early morning hours and removal of the inversion instigated vigorous updraft with rotation due to vertical shear in the upper-level atmosphere. This caused multiple tornadoes to occur from 0220 to 0340 UTC 6 May 2012. When comparing Tateno's climatological temperature and dew-point temperature profile on the day of event, the mid-level atmosphere was moister than typical sounding in the region. This study showed that tornado development in Tsukuba was caused by a combination of (a) topography and potential vorticity anomaly, which increased vorticity over the Kanto Plain; (b) vertical shear, which produced horizontal vortex line; and c) thermal instability, which triggered supercell and tilted the vortex line in the vertical.

Development of stability maps for flashing-induced instability in a passive containment cooling system for iPOWER

  • Lim, Sang Gyu;No, Hee Cheon;Lee, Sang Won;Kim, Han Gon;Cheon, Jong;Lee, Jae Min;Ohk, Seung Min
    • Nuclear Engineering and Technology
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    • v.52 no.1
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    • pp.37-50
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    • 2020
  • A passive containment cooling system (PCCS) has been developed as advanced safety feature for innovative power reactor (iPOWER). Passive systems are inherently less stable than active systems and the PCCS encountered the flashing-induced instability previously identified. The objective of this study is to develop stability maps for flashing-induced instability using MARS (Multi-dimensional Analysis of Reactor Safety) code. Firstly, we conducted a series of sensitivity analysis to see the effects of time step size, nodalization, and alternative MARS user options on the onset of flashing-induced instability. The riser nodalization strongly affects the prediction of flashing in a long riser of the PCCS, while time step size and alternative user options do not. Based on the sensitivity analysis, a standard input and an analysis methodology were set up to develop the stability maps of PCCS. We found out that the calculated equilibrium quality at the exit of the riser as a stability boundary above 5 kW/㎡ was approximately 1.2%, which was in good agreement with Furuya's results. However, in case of a very low heat flux condition, the onset of instability occurred at the lower equilibrium quality. In addition, it was confirmed that inlet throttling reduces the unstable region.

The improvement of Cu metal film adhesion on polymer substrate by the low-power High-frequency ion thruster

  • Jung Cho;Elena Kralkina;Yoon, Ki-Hyun;Koh, Seok-Keun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2000.02a
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    • pp.60-60
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    • 2000
  • The adhesion interface formation between copper and poly(ethylene terephthalate)(PET), poly(methyl methacrylate)(PMMA) and Polyimide films was treated using Ion assisted reaction system to sequential sputter deposition by High-Frequency ion source. The ion beam modification system used a new type of low power HF ion thruster for space application as new low thruster electric propulsion system. Low power HF ion thruster with diameter 100mm gives the opportunity to obtain beams of Ar+ with currents 20~150 mA (current density 0.5~3.5 mA/cm2) and energy 200~2500eV at HF power level 10~150 W. Using Ar as a working gas it is possible to obtain thrust within 3~8 mN. Contact angles for untreated films were over 95$^{\circ}$ and 80 for Pet, 10o for PMMA and 12o for PI samples as a condition of ion assisted reaction at the ion dose of 10$\times$1016 ions/cm2, the ion beam potential of 1.2 keV and 4 ml/min for environmental gas flow rate. 900o peel tests yielded values of 15 to 35 for PET, 18 to 40 and 12 to 36 g/min. respectively. High resolution X-ray photoelectron spectrocopy is the Cls region for Cu metal on these polymer substrates showed increases in C=O-O groups for polymide, whereas PET and PMMA treated samples showed only C=O groups with increase the ion dose. Finally, unstable polymer surface can be changed from hydrophobic to hydrophilic formation such as C-O and C=O that were confirmed by the XPS analysis, conclusionally, the ion assisted reaction is very effective tools to attach reactive ion species to form functional groups on C-C bond chains of PET, PMMA and PI.

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Synthesis of functional ZnO nanoparticles and their photocatalytic properties

  • Nam, Sang-Hun;Kim, Myoung-Hwa;Lee, Sang-Duck;Kim, Min-Hee;Boo, Jin-Hyo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.54-54
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    • 2010
  • Zinc oxide is metal oxide semiconductor with the 3.37 eV bandgap energy. Zinc oxide is very attractive materials for many application fields. Zinc Oxide has many advantages such as high conductivity and good transmittance in visible region. Also it is cheaper than other semiconductor materials such as indium tin oxide (ITO). Therefore, ZnO is alternative material for ITO. ZnO is attracting attention for its application to transparent conductive oxide (TCO) films, surface acoustic wave (SAW), films bulk acoustic resonator (FBAR), piezoelectric materials, gas-sensing, solar cells and photocatalyst. In this study, we synthesized ZnO nanoparticles and defined their physical and chemical properties. Also we studied about the application of ZnO nanoparticles as a photocatalyst and try to find a enhancement photocatalytic activity of ZnO nanorticles.. We synthesized ZnO nanoparticles using spray-pyrolysis method and defined the physical and optical properties of ZnO nanoparticles in experiment I. When the ZnO are exposed to UV light, reduction and oxidation(REDOX) reaction will occur on the ZnO surface and generate ${O_2}^-$ and OH radicals. These powerful oxidizing agents are proven to be effective in decomposition of the harmful organic materials and convert them into $CO_2$ and $H_2O$. Therefore, we investigated that the photocatalytic activity was increased through the surface modification of synthesized ZnO nanoparticles. In experiment II, we studied on the stability of ZnO nanoparticles in water. It is well known that ZnO is unstable in water in comparison with $TiO_2$. $Zn(OH)_2$ was formed at the ZnO surface and ZnO become inactive as a photocatalyst when ZnO is present in the solution. Therefore, we prepared synthesized ZnO nanoparticles that were immersed in the water and dried in the oven. After that, we measured photocatalytic activities of prepared samples and find the cause of their photocatalytic activity changes.

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Numerical Analysis of the Outlet Structure Effect on the Flow and Settling Performance of the Rectangular Clarifiers (장방형 2차 침전지 유출부의 구조가 유동 및 성능에 미치는 영향에 대한 수치 해석적 연구)

  • Kim, Youngchul;Park, Moo Jong;Sheen, Dong-Jin
    • Journal of Korean Society on Water Environment
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    • v.22 no.3
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    • pp.557-565
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    • 2006
  • Proper operation of clarifier partly depends on outlet conditions. Effluent has to be uniformly withdrawn to prevent scouring of settled sludge and carry over of settling sludge from the clarifier. In this paper, 3-D numerical model was employed to analyze the flow and settling performance of the rectangular clarifier. There were two simulation conditions of which in the same clarifier, the first one was assumed to have a 11.5 meter weir length and the second has 8.5 meters. Shape, location and placement of the weir structure were different, but both of those outlets meet weir loading design criteria. Simulation results indicate that clarifier with longer weir generally gave strong and unstable currents at the mid-stream where the weir starts while in the clarifier with short and relatively simple weir, uniform and stable flow was observed in most parts of the settling zone, and especially at the weir region. These flow conditions affected settling performance. Effulent SS concentration from clarifier equipped with the long and complicated placement of weir was 24.5 mg/L, but 7.0 mg/L from the clarfieir having short weir length. From this study, it can be concluded that as reported from other studies, weir loading does not guarantee settling performance and 3-D numerical model can be a useful tool for determining and validating outlet structure.

NONLINEAR CONTROL FOR CORE POWER OF PRESSURIZED WATER NUCLEAR REACTORS USING CONSTANT AXIAL OFFSET STRATEGY

  • ANSARIFAR, GHOLAM REZA;SAADATZI, SAEED
    • Nuclear Engineering and Technology
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    • v.47 no.7
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    • pp.838-848
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    • 2015
  • One of the most important operations in nuclear power plants is load following, in which an imbalance of axial power distribution induces xenon oscillations. These oscillations must be maintained within acceptable limits otherwise the nuclear power plant could become unstable. Therefore, bounded xenon oscillation is considered to be a constraint for the load following operation. In this paper, the design of a sliding mode control (SMC), which is a robust nonlinear controller, is presented.SMCis ameansto control pressurized water nuclear reactor (PWR) power for the load following operation problem in a way that ensures xenon oscillations are kept bounded within acceptable limits. The proposed controller uses constant axial offset (AO) strategy to ensure xenon oscillations remain bounded. The constant AO is a robust state constraint for the load following problem. The reactor core is simulated based on the two-point nuclear reactor model with a three delayed neutron groups. The stability analysis is given by means of the Lyapunov approach, thus the control system is guaranteed to be stable within a large range. The employed method is easy to implement in practical applications and moreover, the SMC exhibits the desired dynamic properties during the entire output-tracking process independent of perturbations. Simulation results are presented to demonstrate the effectiveness of the proposed controller in terms of performance, robustness, and stability. Results show that the proposed controller for the load following operation is so effective that the xenon oscillations are kept bounded in the given region.

The study of Dong-shan(東山) Yao(瑤) marriage culture (동산요족(東山瑤族)의 혼인형태탐구(婚姻形態探究))

  • Xing, Li
    • Korean Journal of Heritage: History & Science
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    • v.37
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    • pp.213-242
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    • 2004
  • "China", "Guang-Xi(廣西)", "Dong-Shan(東山)", a marriage of "Yao-zu(瑤族)" divide mainly into a distinguished family form, but are by a marriage of a traditional general form (a woman getting married) and "Zhaoxu-Hun(招婿婚)", a marriage of "Liangtou-che(兩頭扯)". These two marriage format that mentioned the back is a marriage format to often appear in other Chinese minority race region while being a custom peculiar to "Yao-zu(瑤族)" marriage method. "Zhaoxu-Hun(招婿婚)" provides labor force as a workman while living with a man sinks with "son-in-law" to a woman house, and doing, and it is to carry out a lot of duty as a husband too. "A Liangtou-che(兩頭扯)" marriage is more characteristic, but lives while going around a bilateral family while man and woman each stay in an own house. There are inheritance of property rights to own family, too and obeys anger of he direction family bilateral where if lays a child even if stands up and holds a memorial service. At the same time that these marriages are performed between the same "Yao-zu(瑤族)" near a track, and solution does a distribution problem between a labor problem and the families which were unstable through a mutual marriage for the purpose a compunction "Yao-zu(瑤族)" social network construction into intimacy anger. A general idea is covered he base with in order the porcelain which is a weak race prevent that it is assimilated by external aggression because "Yo-zu(瑤族)" has always received aggression of "a Han-zu(漢族)" too historically, and to keep an ethnic pure blood.

Fabrication of functionally customized fixed prosthesis in a hemimandibulectomy patient with fibular graft using double scan technique: A case report (비골이식을 받은 하악골절제술 환자에서 스캔중첩법을 이용한 기능적 고정성 보철물 제작: 증례 보고)

  • Win, Thaw Thaw;Lee, Du-Hyeong
    • The Journal of Korean Academy of Prosthodontics
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    • v.60 no.2
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    • pp.195-201
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    • 2022
  • Hemimandibulectomy and free fibular flap (FFF) in patients with squamous cell carcinoma could disturb the functional movement of the mandible. The muscular function in the sectioned side was compromised because of the incompetency of the muscle attachment to the mandible, leading to the unstable occlusal contact in the centric and eccentric occlusion. In this report, we present a case of a 63-year-old male who underwent cancer surgery and reconstruction with fibular graft, and he needed a fixed dental prosthesis to restore tooth loss in the anterior region. Occlusal contact change and instability were found according to the bite force in the centric relation and eccentric movement. This case report aimed to present the prosthetic procedure for fabricating the functionally optimized fixed prosthesis wherein the functionally generated path (FGP) technique and digital imaging method were applied to replicate mandibular movement and vertical dimension of the patient.

Research on Relay Selection Technology Based on Regular Hexagon Region Segmentation in C-V2X

  • Li, Zhigang;Yue, Xinan;Wang, Xin;Li, Baozhu;Huang, Daoying
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.16 no.9
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    • pp.3138-3151
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    • 2022
  • Traffic safety and congestion are becoming more and more serious, especially the frequent occurrence of traffic accidents, which have caused great casualties and economic losses. Cellular Vehicle to Everything (C-V2X) can assist in safe driving and improve traffic efficiency through real-time information sharing and communication between vehicles. All vehicles communicate directly with Base Stations (BS), which will increase the base station load. And when the communicating vehicles are too far apart, too fast or there are obstacles in the communication path, the communication link can be unstable or even interrupted. Therefore, choosing an effective and reliable multi-hop relay-assisted Vehicle to Vehicle (V2V) communication can not only reduce the base station load and improve the system throughput but also expand the base station coverage and improve the communication quality of edge vehicles. Therefore, a communication area division scheme based on regular hexagon segmentation technology is proposed, a relay-assisted V2V communication mechanism is designed for the divided communication areas, and an efficient communication link is constructed by selecting the best relay node. Simulation results show that the scheme can improve the throughput of the system by nearly 55% and enhance the robustness of the V2V communication link.