• 미생물학회지
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• 제40권2호
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• pp.65-72
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• 2004

진핵생물의 특정 염기배열을 원핵생물 내에서 증폭시킬 때 불안정성이 비교적 빈번히 관찰되어진다. 특히 long inverted repeats나 AT-rich sequences그리고 Z-DNA와 같은 구조를 지닌 염기배열은 대장균 내에서 매우 불안정하다. 이러한 염기서열은 대장균 내에서 부분적으로 결실되거나 완전히 손실된다. 본 연구실에서 human SCKI 유전자에 존재하는 몇 개의 tandem repeat (TR)에 대하여 다형성을 조사하였을 때, 어떤 TR 부분은 플라스미드로부터 빈번히 결실되어 그에 대한 염기서열 결정이 어려웠다. 그 결과 이러한 부분은 클론닝 될 수 없는 염기서열로 남게 되었다. 본 연구에서는 클론닝이 어려운 두 개의 TR 영역을 저온에서 클론닝하고 nebulizer나 sonicator를 이용하여 두 개의 library를 만들어 DNA 염기서열을 결정하였다. 이러한 연구는 복잡한 고등생물의 게놈연구에서 불안정한 게놈부분의 염기서열을 결정하는데 도움을 줄 것으로 사료된다.

• 대한기계학회논문집B
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• 제23권11호
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• pp.1434-1443
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• 1999

The turbulent fluctuations of temperature and two components of velocity have been measured with hot- and cold-wires in the Thermally Stratified Wind Tunnel(TSWT). Using the fin-tube heat exchanger type heaters and the neural network control algorithm, both stable ($dT/dz=109.4^{\circ}C$) and unstable ($dT/dz=-49.1^{\circ}C$) stratifications were realized. An ambient air jet was issued normally into the cross flow($U_{\infty}=1.0 m/s$) from a round nozzle(d = 6 mm) flushed at the bottom waII of the wind tunnel with the velocity ratio of $5.8(U_{jet}/U_{\infty})$. The characteristics of turbulent dispersion in the cross flow jet are found to change drastically depending on the thermal stratification. Especially, in the unstable condition, the vertical velocity fluctuation increases very rapidly at downstream of jet. The fluctuation velocity spectra and velocity-temperature cospectra along the jet centerline were obtained and compared. In the case of stable stratification, the heat flux cospectra changes Its sign from a certain point at the far field because of the restratification phenomenon. It is inferred that the main reason in the difference between the vertical heat fluxes is caused by the different length scales of the large eddy motions. The turbulent kinetic energy and scalar dissipation rates were estimated using partially non-isotropic and isotropic turbulent approximation. In the unstable case, the turbulent energy dissipation decreases more rapidly with the downstream distance than in the stable case.

• 한국공간구조학회논문집
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• 제20권4호
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• pp.149-158
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• 2020

The governing equation for a dome-type shallow spatial truss subjected to a transverse load is expressed in the form of the Duffing equation, and it can be derived by considering geometrical non-linearity. When this model under constant load exceeds the critical level, unstable behavior is appeared. This phenomenon changes sensitively as the number of free-nodes increases or depends on the imperfection of the system. When the load is a periodic function, more complex behavior and low critical levels can be expected. Thus, the dynamic unstable behavior and the change in the critical point of the 3-free-nodes space truss system were analyzed in this work. The 4-th order Runge-Kutta method was used in the system analysis, while the change in the frequency domain was analyzed through FFT. The sinusoidal wave and the beating wave were utilized as the periodic load function. This unstable situation was observed by the case when all nodes had same load vector as well as by the case that the load vector had slight difference. The results showed the critical buckling level of the periodic load was lower than that of the constant load. The value is greatly influenced by the period of the load, while a lower critical point was observed when it was closer to the natural frequency in the case of a linear system. The beating wave, which is attributed to the interference of the two frequencies, exhibits slightly more behavior than the sinusoidal wave. And the changing of critical level could be observed even with slight changes in the load vector.

• 제어로봇시스템학회논문지
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• 제22권5호
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• pp.346-352
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• 2016

This paper proposes an adaptive robust control method for an acrobot system in the presence of input disturbance. The acrobot system is a typical example of the underactuated system with complex nonlinearity and strong dynamic coupling. Also, disturbance can cause limit cycle phenomenon which appears in the acrobot system around the desired unstable equilibrium point. To minimize the effect of the disturbance, we apply a fuzzy disturbance estimation method for the swing-up and balancing control of the acrobot system. In this paper, both disturbance observer and controller for the acrobot system are designed and verified through mathematical proof and simulations.

• 대한기계학회논문집B
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• 제39권1호
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• pp.89-95
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• 2015

터보압축기의 서지는 압축기의 불안정 운전영역으로 주로 소음과 맥동을 유발하며 수회 지속될시 압축기 시스템 전반에 걸쳐 막대한 피해를 입힌다. 압축기의 안전한 운전을 위해서는 서지현상에 대한 특성파악과 제어전략 수립이 중요하다. 서지현상의 제어는 주로 압축가스의 통과유동량 증대, 필요한 헤드 저감, 압축기 회전수 감속, 가스의 바이패스를 통하여 이루어진다. 본 연구에서는 차량용 터보차저에 서지를 유발했을 때 각 운전 구간별 발생되는 압력변동 특성을 연구하고자 한다. 서지 특성 확인을 위한 파라미터는 압축기 입구 유량과 출구 유량, 관경, 회전수 등으로 선정하였다. 입구단과 출구단 유량을 조절하여 서지 압력 변동을 조사한 결과, 출구단 보다는 입구단 유량의 급격한 변화가 서지와 압축기 내구에 더 영향이 크다는 것을 확인하였다.

• 한국환경과학회지
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• 제4권2호
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• pp.121-139
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• 1995

Based on the Results of Marine Meteorological and Oceanographical Observations (1966 -1987), the phenomenon of chimney is found as a candidate for the formation of the Japan Sea Proper Water (JSPW). The chimney phenomenon occurs twelve times Inuring 1966∼ 1987. The water types in the chimney denoting the deep convection are similar to those of the JSPW 0∼ 1℃ in potential temperature, 34.0∼34.1 ‰ in salinity and 68∼80 cl/t in potential thermosteric anomaly from the sea surface to the deep layer. The static stabilities in the chimney stations are unstable or neutral. This indicates that the winter time convection occurs. The JSPW sunken from the surface layer of chimney in winter spreads out under the Tsushima Warm Current area, following the isosteric surface of about 76 cl/t in Potential thermosteric anomaly. The formation of the deep water of the JSPW is mainly affected by the cooling of the sea surface than the evaporation of winds because the temperature and the salinity on the isoteric surface of about 76 cl/t in potential thermosteric anomaly ate cold and low The phenomenon of chimney occurred in here and there of the area in the north of 40" 30'N, west of 138" E. This suggests that the deep water of the JSPW is formed not in a limited area but probably in the overall region of the northern open ocean.

• 한국공간구조학회논문집
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• 제4권2호
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• pp.63-71
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• 2004

트러스형 공간 구조물은 무주의 대공간을 덮을 수 있는 장점과 구조적 성질이 동일한 등가 연속체 쉘 로 치환하여도 비교적 정확한 해를 얻을 수 있다는 장점으로 인해 21세기 첨단 구조물의 한 장인 초대형 구조물 분야에 많이 활용되고 있으며, 효율적인 부재의 이용과 대량생산의 가능성으로 인해 많은 발전을 해 왔다. 그러나 이러한 쉘 형태의 공간 구조물은 구조 거동의 특성상 주로 구조안정문제가 구조설계에서 해결해야하는 핵심적인 기술력이 되며, 이를 어떻게 해결하여야 할 것인가의 문제는 아직도 많은 연구자들에게 난제로 남아 있다. 즉, 연속체 쉘 구조의 원리에서 긴 경간을 얇게 만들면, 뜀좌굴과 분기좌굴같은 불안정 거동이 나타나게 되며, 이러한 쉘형 구조 시스템에서 구조 불안정 문제의 특징은 초기 조건에 매우 민감하게 반응한다는 것이고, 이런 문제들은 수학적으로 비선형 문제에 귀착하게 된다. 따라서, 본 논문에서는 공간 프레임형 구조물의 불안정 현상을 살펴보기 위하여, 다양한 파라메타중 초기불완전량과 rise-span 비가 트러스 구조물의 불안정 현상에 미치는 영향을 알아보고자 하며, 이를 위해 1-자유절점 공간구조물, 2-자유절점 공간구조물, 다-자유절점 공간구조물을 예제로 채택하여 불안정 거동을 살펴보고자 한다.

• 한국실내디자인학회논문집
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• 제16호
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• pp.106-115
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• 1998

Latest modernism shows general idea of phenomenal space through nature of place motility and experience sinceded from standard form and it has general idea of space. therefore this study presents the tension and the relaxation as a method looking for spatal change and value that in spatial experience. For the analysis of work the Conference Pavillon of Tadoa Ando in the furniture company in Vitra Fire Station of Zaha Hidid is analyzed in spatial experence. As the result the result the systematic straight mixture that has a superrimotion with long retangle which the space acts independently and is shown in the drawing of absolutism is formed and collapses theregularity of construction form by decomposition annexation mixing collision and ambivalence. The spatial experiance in this unstable form changes the grade of rank of space by opposite phenomenon by the change of spatial flowing the disorted and vague space that is occurred by superrimotion which has no direction and change of lirht and place and consolidates dynamic strain makes possible to construct against the gravity. And it also creates experienced space thatis reaxed with maintenance of organization of unity harmony, and stsbility.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2004년도 봄 학술발표회 논문집
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• pp.1-8
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• 2004

For the linearized differential algebraic equation of the nonlinear constrained system, exact initial values of the acceleration are needed to solve itself. It may be very troublesome to perform the inverse operation for obtaining the incremental quantities since the mass matrix contains the zero element in the diagonal. This fact makes the mass matrix impossible to be positive definite. To overcome this singularity phenomenon the mass matrix needs to be modified to allow the feasible application of predictor and corrector in the iterative computation. In this paper the proposed numerical algorithm based on the modified mass matrix combines the conventional implicit algorithm, Newton-Raphson method and Newmark method. The numerical example presents reliabilities for the proposed algorithm via comparisons of the 4th order Runge-kutta method. The proposed algorithm seems to be satisfactory even though the acceleration, Lagrange multiplier, and energy show unstable behaviour. Correspondingly, it provides one important clue to another algorithm for the enhancement of the numerical results.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.1166-1171
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• 2004

The combustion instability acts as a serious obstacle for the lean premixed combustion of gas turbine and even causes the fatal damage to the combustor and whole system. In this experiment, the pressure fluctuation is highly related to the stabilizing position of flame and fuel injection location. The fuel injection location is connected with the convection time of the fresh mixture, which is important time scale to refresh the mixtures near the flame stabilization location. The flame is extremely unstable when the alternative stabilization occurs and bulk mode frequency (${\sim}10Hz$) of pressure fluctuation is observed in this condition. It was found that the convection time scale of the fresh reactant coincided with the time scale of the bulk mode fluctuation. Hence this phenomenon results from the local equivalence ratio change caused by the pressure fluctuation induced by thermo-acoustic effects.