• 제목/요약/키워드: Unrecorded Fungi

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목본식물의 잎에서 분리된 국내 미기록 내생균: Neofusicoccum mangiferae and Thyronectria cucurbitula (Neofusicoccum mangiferae and Thyronectria cucurbitula: Unrecorded Endophytic Fungi Isolated from the Leaves of Woody Plants in Korea)

  • 이종철;박혁;엄안흠
    • 한국균학회지
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    • 제48권4호
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    • pp.415-421
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    • 2020
  • 본 연구에서는 울릉도 및 여수에 서식하는 목본식물의 잎을 채취하고 내생균을 분리하였다. 분리된 균주는 배양 특성과 분생포자 등의 형태성 및 rDNA의 internal transcribed spacer 영역, large subunit 영역, 그리고 β-tubulin 영역의 DNA 염기서열을 분석하여 동정하였다. 연구 결과 국내 미기록 내생균 2종을 확인하였으며, 확인된 종은 Neofusicoccum mangiferae과 Thyronectria cucurbitula이다. 확인된 미기록종 균주의 형태적 특성 및 분자생물학적 계통분석의 결과에 대해 서술하였다.

한국에 서식하는 침엽수의 잎과 난초과 식물의 뿌리에서 분리한 5종의 국내 미기록 내생균 (Notes on Five Unrecorded Endophytic Fungi Isolated from Coniferous Leaves and Orchid Roots in Korea)

  • 박혁;이봉형;배유라;김동여;엄안흠
    • 한국균학회지
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    • 제44권4호
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    • pp.365-370
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    • 2016
  • 본 연구에서는 잣나무, 눈측백의 침엽과 자란의 뿌리에서 식물에 공생하는 내생균을 분리하여 동정하였다. 이를 위해 specific primer인 ITS1F와 ITS4를 이용하여 internal transcribed spacer (ITS) rDNA영역과 primer LR0R과 LR 16을 이용하여 large subunit rDNA 영역을, primer Bt2a과 Bt2b를 이용하여 ${\beta}$-tubulin 영역을, 그리고 EF-1 primer를 이용하여 translation elongation factor 지역의 염기서열을 동시에 분석하였다. 연구 결과 Colletotrichum simmondsii, Fusarium sterilihyphosum, Diatrypella pulvinata, Ochroconis globalis, Sphaeria chrysosperma 등 국내 미기록 균주 5종을 분리하여 형태적, 분자생물학적 동정 결과를 서술하였다.

Diversity of Nigrospora (Xylariales, Apiosporaceae) Species Identified in Korean Macroalgae Including Five Unrecorded Species

  • Wonjun Lee;Dong-Geon Kim;Rekhani H. Perera;Ji Seon Kim;Yoonhee Cho;Jun Won Lee;Chang Wan Seo;Young Woon Lim
    • Mycobiology
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    • 제51권6호
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    • pp.401-409
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    • 2023
  • Nigrospora (Xylariales, Apiosporaceae) consists of species of terrestrial plant endophytes and pathogens. Nigrospora has also been reported in marine environments such as mangroves, sea fans, and macroalgae. However, limited research has been conducted on Nigrospora associated with macroalgae. Here, we isolated Nigrospora species from three types of algae (brown, green, and red algae) from Korean islands (Chuja, Jeju, and Ulleung) based on phylogenetic analyses of multigenetic markers: the internal transcribed spacers (ITS), beta-tubulin (BenA), and translation elongation factor 1 (TEF1-α). A total of 17 Nigrospora strains were isolated from macroalgae and identified as nine distinct species. The majority of Nigrospora species (seven) were found on brown algae, followed by red algae (three), and then green algae (two). To our understanding, this study represents the first account of N. cooperae, N. covidalis, N. guilinensis, N. lacticolonia, N. osmanthi, N. pyriformis, and N. rubi occurring in marine environments. Additionally, this study provides the first report of the occurrence of N. cooperae, N. covidalis, N. guilinensis, N. lacticolonia, and N. osmanthi in South Korea. This study will provide valuable insights for future research exploring the functions of fungi in macroalgal communities.

Taxonomical Studies on Korean Higher Fungi(I)

  • Park, Wan-Hee;Kim, Tae-Hee;Kim, Byong-Kak;Ro, Ihl-Hyeob
    • 생약학회지
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    • 제16권2호
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    • pp.61-64
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    • 1985
  • To find new species of Basidiomycetes in Korea, 15 species were collected in the medicinal plants garden of Sook Myung Women’s University during the period from May to November, 1983. Of them, 11 species were identified and classified into 10 genera and seven families. Among these species, Macrocystidia cucumis(Pers. ex Fr.) Heim and Melanoleuca verrucipes (Fr.) Singer were found to be unrecorded species in Korea. Also their two genera are newly found ones in Korea. They were named 'Bahm Saek Min $B\breve{u}s\breve{u}t$' and 'Hin Bol Lock $B\breve{u}s\breve{u}t$' respectively and their characteristics were here reported.

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Marine Athrinium spp. Isolated from Sargassum sp. (Brown Algae) in Jeju Island and Unrecorded Species in Korea

  • Jang, Seokyoon;Hong, Joo-Hyun;Heo, Young Mok;Kim, Jae-Jin
    • 한국균학회지
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    • 제44권4호
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    • pp.259-262
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    • 2016
  • Arthrinium (Ascomycota, Apiosporaceae) is a major marine fungal genus. Three Arthrinium species were reported previously, but not fully described. We isolated the two species A. arundinis and A. saccharicola from Sargassum sp. brown algae in Jeju Island, Korea. These species have not been previously reported in Korea. We described these species in detail and performed a phylogenetic analysis based on the nucleotide sequences of the EF1-${\alpha}$ and ${\beta}$-tubulin genes.

한국산(韓國産) 고등균류(高等菌類)의 분류학적(分類學的) 연구(硏究)(II) -Cordyceps속(屬)의 미기록(未記錄) 2종(種)- (Taxonomic Investigations on Korean Higher Fungi(II) -Two Unrecorded Species of the Genus Cordyceps in Korea-)

  • 임정한;김병각
    • 한국균학회지
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    • 제1권1호
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    • pp.13-16
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    • 1973
  • 맥각균과(麥角菌科)의 Cordyceps속(屬)은 오래전부터 약용(藥用)으로 쓰여졌으며 현재(現在)까지 한국(韓國)에서 Cordyceps sobolifera, C. militaris 및 C. nutans의 3종(種)만이 보고(報告)되어 있다. 저자(著者)들은 Cordyceps tricentri Yasuda 및 C. sphecocephala(Klotz) Mass의 미기록(未記錄) 2종(種)을 한국(韓國)에서 채집하여 보고(報告)한다.

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Phylogenetic Status of an Unrecorded Species of Curvularia, C. spicifera, Based on Current Classification System of Curvularia and Bipolaris Group Using Multi Loci

  • Jeon, Sun Jeong;Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • Mycobiology
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    • 제43권3호
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    • pp.210-217
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    • 2015
  • A seed-borne fungus, Curvularia sp. EML-KWD01, was isolated from an indigenous wheat seed by standard blotter method. This fungus was characterized based on the morphological characteristics and molecular phylogenetic analysis. Phylogenetic status of the fungus was determined using sequences of three loci: rDNA internal transcribed spacer, large ribosomal subunit, and glyceraldehyde 3-phosphate dehydrogenase gene. Multi loci sequencing analysis revealed that this fungus was Curvularia spicifera within Curvularia group 2 of family Pleosporaceae.

Eight Previously Unreported Species of Fungi Identified in Mt. Manggyeong, Korea

  • Lee, Jin Sung;Kim, Changmu;Lee, Hyang Bum
    • 한국균학회지
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    • 제42권4호
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    • pp.344-348
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    • 2014
  • A total of 118 fungal specimens were collected from Mt. Manggyeong, Chungnam province, Korea between July and October 2014. All specimens were identified to the species level using a combination of morphological characteristics and DNA sequencing. Out of 118 specimens, eight species, Amanita manginiana, A. pilosella, Calvatia holothurioides, Cantharellus tabernensis, Dicephalospora rufocornea, Gymnopus menehune, Marasmius brunneospermus, and Russula cerolens, have not been previously reported in Korea.

Neocosmospora rubicola, an Unrecorded Endophytic Fungus Isolated from Roots of Glycyrrhiza uralensis in Korea

  • Kim, Jin-Hee;Kim, Dong-Yeo;Park, Hyeok;Cho, Jae Hee;Eom, Ahn-Heum
    • 한국균학회지
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    • 제45권1호
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    • pp.63-67
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    • 2017
  • Through a survey of symbiotic endophytic fungi, we isolated an endophyte fungal strain from the roots of Glycyrrhiza uralensis in Korea. The isolated fungal strain was identified using its morphological characteristics and through phylogenetic analysis of the internal transcribed spacer, the large subunit rDNA region, and the translation elongation factor region. The strain was identified as Neocosmospora rubicola. This species has not been previously reported in Korea. In this study, we report its isolation from the roots of Glycyrrhiza uralensis in Korea, followed by the characterization and identification of the strain.

Isolation and Characterization of Three Unrecorded Zygomycete Fungi in Korea: Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans

  • Nguyen, Thuong T.T.;Choi, Young-Joon;Lee, Hyang Burm
    • Mycobiology
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    • 제45권4호
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    • pp.318-326
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    • 2017
  • In a survey of undiscovered taxa in Korea, three zygomycete fungal strains-EML-W31, EML-HGD1-1, and EML-RUS1-1-were isolated from freshwater, grasshopper fecal, and soil samples in Korea. On the basis of the morphological characteristics and phylogenetic analysis of internal transcribed spacer and 28S rDNA, the isolates of EML-W31, EML-HGD1-1, and EML-RUS1-1 were confirmed to be Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans, respectively. These species have not been previously described in Korea.