The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Neofusicoccum mangiferae and Thyronectria cucurbitula: Unrecorded Endophytic Fungi Isolated from the Leaves of Woody Plants in Korea

목본식물의 잎에서 분리된 국내 미기록 내생균: Neofusicoccum mangiferae and Thyronectria cucurbitula

  • Lee, Jong-Chul (Department of Biology Education, Korea National University of Education) ;
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 이종철 (한국교원대학교 생물교육과) ;
  • 박혁 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2020.11.06
  • Accepted : 2020.12.09
  • Published : 2020.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

We isolated endophytic fungi from the leaves of woody plants inhabiting the Ulleungdo Island and Yeosu in South Korea. The fungal strains were identified based on their morphological characteristics in conjunction with phylogenetic analysis, which was performed using sequences of the internal transcribed spacer, large subunit rDNA, and β-tubulin DNA. We confirmed the presence of Neofusicoccum mangiferae and Thyronectria cucurbitula, two endophytic fungal species that had not been previously recorded in Korea. In this report, we describe the morphological characteristics of these fungal strains and results of the DNA-based phylogenetic analysis.

본 연구에서는 울릉도 및 여수에 서식하는 목본식물의 잎을 채취하고 내생균을 분리하였다. 분리된 균주는 배양 특성과 분생포자 등의 형태성 및 rDNA의 internal transcribed spacer 영역, large subunit 영역, 그리고 β-tubulin 영역의 DNA 염기서열을 분석하여 동정하였다. 연구 결과 국내 미기록 내생균 2종을 확인하였으며, 확인된 종은 Neofusicoccum mangiferae과 Thyronectria cucurbitula이다. 확인된 미기록종 균주의 형태적 특성 및 분자생물학적 계통분석의 결과에 대해 서술하였다.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the ministry of Environment (MOE) of the Republic of Korea (NIBR201902202).

References

  1. Carroll G. Fungal endophytes in stems and leaves: From latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  2. Gao FK, Dai CC, Liu XZ. Mechanisms of fungal endophytes in plant protection against pathogens. Afr J Microbiol Res 2010;4:1346-51.
  3. Clay K. Fungal endophytes of grasses: A defensive mutualism between plants and fungi. Ecology 1988;69:10-6. https://doi.org/10.2307/1943155
  4. Bouton J, Gates R, Belesky D, Owsley M. Yield and persistence of tall fescue in the southeastern coastal plain after removal of its endophyte. Agronomy 1993;85:52-5. https://doi.org/10.2134/agronj1993.00021962008500010011x
  5. Park H, Choi YJ, Eom AH. Characterization of six novel endophytic fungi isolated from leaves of plants inhabiting Jeju island. Kor J Mycol 2018;46:405-14.
  6. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547-9. https://doi.org/10.1093/molbev/msy096
  7. Sydow H. Fungi Indiae orientalis, pars V. Ann Mycol. 1916;14:177-220.
  8. Crous PW, Slippers B, Wingfield MJ, Rheeder J, Marasas WF, Philips AJ, Alves A, Burgess T, Barber P, Groenewald JZ. Phylogenetic lineages in the Botryosphaeriaceae. Stud Mycol 2006;55:235-53. https://doi.org/10.3114/sim.55.1.235
  9. Phillips AJ, Alves A, Abdollahzadeh J, Slippers B, Wingfield MJ, Groenewald JZ, Crous PW. The Botryosphaeriaceae: Genera and species known from culture. Stud Mycol 2013;76:51-167. https://doi.org/10.3114/sim0021
  10. Dissanayake AJ, Zhang W, Li X, Zhou Y, Chethana T, Chukeatirote E, Hyde KD, Yan J, Zhang G, Zhao W. First report of Neofusicoccum mangiferae associated with grapevine dieback in China. Phytopathol Mediterr 2015;54:414-9.
  11. Tode F. Fungi Mecklenburgenses selecti, Fasciculus 2. F. Lemke, Luneburg 1791.
  12. Fries E. Summa vegetabilium Scandinaviae Vol. 2. Stockholm, Leipzig: Bonnier 1849.
  13. Jaklitsch W, Voglmayr H. Persistent hamathecial threads in the Nectriaceae, Hypocreales: Thyronectria revisited and re-instated. Persoonia 2014;33:182-211. https://doi.org/10.3767/003158514X685211
  14. Mihal I, Cicak A, Bucinova K. Distribution, ecology and taxonomical notes to species of genera Hypomyces and Nectria (Hypocreales) in Slovakia. Mikologiia I Fitopatologiia 2007;41:242.
  15. Hirooka Y, Rossman AY, Samuels GJ, Lechat C, Chaverri P. A monograph of Allantonectria, Nectria, and Pleonectria (Nectriaceae, Hypocreales, Ascomycota) and their pycnidial, sporodochial, and synnematous anamorphs. Stud Mycol 2012;71:1-210. https://doi.org/10.3114/sim0001
  16. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  17. Moncalvo JM, Lutzoni FM, Rehner SA, Johnson J, Vilgalys R. Phylogenetic relationships of agaric fungi based on nuclear large subunit ribosomal DNA sequences. Syst Biol 2000;49:278-305. https://doi.org/10.1080/10635159950173852
  18. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323-30. https://doi.org/10.1128/aem.61.4.1323-1330.1995